BuceA.00057.f

Putative oligosaccharide ABC transporter ATP-binding protein

CENTER ID: BuceA.00057.f
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00057.f.A1.GE33169 Full length( BuceA.00057.f ) 1 304
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1703
RefSeq: YP_002230775.1
UniProt: B4E8S0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVAPIDFRN VEKRYGDKLV VNGLSFNVKA GECYGLLGPN GAGKTTTLKM LLGLAHPDAG TISLCGEPVP SRARHARQRV GVVPQFDNLD PDFTVRENLL VFSRYFGMSA HAARTLVPPL LEFAKLENKA DAKVGELSGG MKRRLTLARA LVNDPDVLVL DEPTTGLDPQ ARHLMWERLR SLLARGKTIL ITTHFMEEAE RLCDRLCVIE EGRKIAEGAP HALIESEIGC DVIEIYGPDP AALRDELSAF AKHTEISGET LFCYVSDPEP LSARLKGRAG LRYLHRPANL EDVFLRLTGR EMQD
NT Sequence
ATGTCCGTCG CACCGATCGA TTTCCGCAAC GTCGAAAAGC GCTATGGCGA CAAGCTGGTC GTCAACGGTC TGTCCTTCAA CGTGAAGGCC GGCGAATGCT ATGGCCTGCT CGGGCCGAAC GGCGCCGGCA AGACCACGAC GCTGAAGATG CTGCTCGGCC TCGCGCACCC CGATGCCGGC ACCATTTCGC TATGCGGCGA ACCGGTTCCA TCGCGGGCGC GACATGCACG CCAGCGGGTC GGCGTCGTCC CGCAGTTCGA CAATCTCGAC CCCGACTTCA CCGTACGCGA GAACCTGCTC GTCTTCAGCC GCTATTTCGG GATGTCGGCG CACGCCGCAC GCACGCTCGT GCCGCCGCTG CTCGAATTCG CGAAGCTGGA AAACAAGGCC GACGCGAAGG TCGGCGAGCT GTCGGGCGGC ATGAAGCGCC GCCTCACGCT CGCCCGTGCG CTCGTCAACG ATCCCGACGT GCTGGTGCTC GACGAGCCGA CGACGGGCCT CGATCCGCAG GCGCGGCACC TGATGTGGGA GCGGCTGCGT TCGCTGCTTG CGCGCGGCAA GACGATCCTG ATCACCACGC ACTTCATGGA AGAAGCCGAG CGGCTGTGCG ATCGGCTGTG CGTGATCGAG GAAGGCCGCA AGATCGCCGA AGGCGCGCCG CATGCGCTGA TCGAATCGGA GATCGGCTGC GACGTGATCG AGATCTACGG GCCCGATCCG GCCGCGCTGC GCGACGAGTT GTCGGCGTTC GCGAAGCACA CGGAGATCAG CGGCGAGACG CTGTTCTGCT ATGTGAGCGA CCCCGAACCG CTCAGCGCGC GCCTGAAGGG CCGCGCGGGG TTGCGCTATC TGCATCGGCC GGCCAATCTG GAAGATGTGT TCCTGCGGCT CACGGGCCGC GAAATGCAGG AC
Details for BuceA.00057.f.A1.GE33169
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMSVAPID FRNVEKRYGD KLVVNGLSFN VKAGECYGLL GPNGAGKTTT LKMLLGLAHP DAGTISLCGE PVPSRARHAR QRVGVVPQFD NLDPDFTVRE NLLVFSRYFG MSAHAARTLV PPLLEFAKLE NKADAKVGEL SGGMKRRLTL ARALVNDPDV LVLDEPTTGL DPQARHLMWE RLRSLLARGK TILITTHFME EAERLCDRLC VIEEGRKIAE XAPHALIESE IGCDVIEIYG PDPAALRDEL SAFAKHTEIS GETLFCYVSD PEPLSARLKG RAGLRYLHRP ANLEXVFLXL TGXXMQX
Validated NT Sequence
tnnnannnat accntggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgt ccgtcgcacc gatcgatttc cgcaacgtcg aaaagcgcta tggcgacaag ctggtcgtca acggtctgtc cttcaacgtg aaggccggcg aatgctatgg cctgctcggg ccgaacggcg ccggcaagac cacgacgctg aagatgctgc tcggcctcgc gcaccccgat gccggcacca tttcgctatg cggcgaaccg gttccatcgc gggcgcgaca tgcacgccag cgggtcggcg tcgtcccgca gttcgacaat ctcgaccccg acttcaccgt acgcgagaac ctgctcgtct tcagccgcta tttcgggatg tcggcgcacg ccgcacgcac gctcgtgccg ccgctgctcg aattcgcgaa gctggaaaac aaggccgacg cgaaggtcgg cgagctgtcg ggcggcatga agcgccgcct cacgctcgcc cgtgcgctcg tcaacgatcc cgacgtgctg gtgctcgacg agccgacgac gggcctcgat ccgcaggcgc ggcacctgat gtgggagcgg ctgcgttcgc tgcttgcgcg cggcaagacg atcctgatca ccacgcactt catggaagaa gccgagcggc tgtgcgatcg gctgtgcgtg atcgaggaag gccgcaagat cgccgaangc gcgccgcatg cgctgatcga atcggagatc ggctgcgacg tgatcgagat ctacgggccc gatccggccg cgctgcgcga cgagttgtcg gcgttcgcga agcacacgga gatcagcggc gagacgctgt tctgctatgt gagcgacccc gaaccgctca gcgcgcgcct gaagggccgc gcggggttgc gctatctgca tcggccggcc aatctggaag angtgttcct gcngctcacg ggcnnnnaaa tgcagnacta ataaacagca cgaacaagtt ctgcagccnn ctnctcgagg atccggctgc tancaaagcc cnnaangnag cngnnntnng ctgctg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVAPIDFR NVEKRYGDKL VVNGLSFNVK AGECYGLLGP NGAGKTTTLK MLLGLAHPDA GTISLCGEPV PSRARHARQR VGVVPQFDNL DPDFTVRENL LVFSRYFGMS AHAARTLVPP LLEFAKLENK ADAKVGELSG GMKRRLTLAR ALVNDPDVLV LDEPTTGLDP QARHLMWERL RSLLARGKTI LITTHFMEEA ERLCDRLCVI EEGRKIAEGA PHALIESEIG CDVIEIYGPD PAALRDELSA FAKHTEISGE TLFCYVSDPE PLSARLKGRA GLRYLHRPAN LEDVFLRLTG REMQD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgtcgca ccgatcgatt tccgcaacgt cgaaaagcgc tatggcgaca agctggtcgt caacggtctg tccttcaacg tgaaggccgg cgaatgctat ggcctgctcg ggccgaacgg cgccggcaag accacgacgc tgaagatgct gctcggcctc gcgcaccccg atgccggcac catttcgcta tgcggcgaac cggttccatc gcgggcgcga catgcacgcc agcgggtcgg cgtcgtcccg cagttcgaca atctcgaccc cgacttcacc gtacgcgaga acctgctcgt cttcagccgc tatttcggga tgtcggcgca cgccgcacgc acgctcgtgc cgccgctgct cgaattcgcg aagctggaaa acaaggccga cgcgaaggtc ggcgagctgt cgggcggcat gaagcgccgc ctcacgctcg cccgtgcgct cgtcaacgat cccgacgtgc tggtgctcga cgagccgacg acgggcctcg atccgcaggc gcggcacctg atgtgggagc ggctgcgttc gctgcttgcg cgcggcaaga cgatcctgat caccacgcac ttcatggaag aagccgagcg gctgtgcgat cggctgtgcg tgatcgagga aggccgcaag atcgccgaag gcgcgccgca tgcgctgatc gaatcggaga tcggctgcga cgtgatcgag atctacgggc ccgatccggc cgcgctgcgc gacgagttgt cggcgttcgc gaagcacacg gagatcagcg gcgagacgct gttctgctat gtgagcgacc ccgaaccgct cagcgcgcgc ctgaagggcc gcgcggggtt gcgctatctg catcggccgg ccaatctgga agatgtgttc ctgcggctca cgggccgcga aatgcaggac taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa