SSGCID: Target Page

BuceA.00057.b

Putative branched-chain amino acid ABC transporter ATP-binding protein

CENTER ID:	BuceA.00057.b
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00057.b.A1.GE33165	Full length( BuceA.00057.b )		1	251

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.19
RefSeq:	YP_002229180.1
UniProt:	B4E573

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKHSEREERE LSGVESGTPA LEITGLEAWY GESHILHGVD LTVHRGEVVT LLGRNGAGRT TTLRAIMGLT GRRSGSIKVA GHETIGLATH RIAHYGIGYC PEERGIFSSL SCEENLMLPP LIGSREHAMS LDEIYAMFPN LASRRQSQGT RLSGGEQQML AVARILRTGA NLLLLDEISE GLAPVIVQAL ARMIVALKAR GYTIVMVEQN FRFAAPLADR FYVMEHGSIV EHFRASELEG KMPTLHDLLG V

NT Sequence

ATGAAGCACA GCGAACGGGA GGAACGCGAA TTGAGCGGCG TCGAGAGCGG TACGCCTGCG CTGGAGATCA CGGGCCTCGA GGCCTGGTAC GGGGAATCCC ACATCTTGCA CGGTGTCGAC CTGACGGTGC ATCGCGGCGA GGTCGTCACG CTGCTCGGCC GCAACGGCGC GGGCCGCACG ACGACGCTGC GCGCGATCAT GGGCCTCACG GGCCGCCGCA GCGGGTCGAT CAAGGTCGCC GGCCACGAGA CGATCGGCCT CGCGACGCAC CGCATCGCGC ATTACGGCAT CGGTTACTGC CCGGAGGAGC GCGGGATCTT CTCGAGCCTG TCGTGCGAGG AGAACCTGAT GCTGCCGCCG CTGATCGGGT CGCGCGAACA CGCGATGTCG CTCGACGAGA TCTACGCGAT GTTCCCGAAC CTCGCGTCGC GCCGGCAGAG CCAGGGCACG CGGCTGTCCG GTGGCGAGCA GCAGATGCTC GCGGTCGCGC GGATCTTGCG CACCGGCGCG AACCTGCTGC TGCTCGACGA GATTTCCGAG GGCCTCGCGC CGGTGATCGT GCAGGCGCTC GCGCGGATGA TCGTCGCGCT GAAGGCGCGC GGCTACACGA TCGTGATGGT CGAACAGAAT TTCCGCTTCG CCGCGCCGCT CGCCGACCGC TTCTACGTGA TGGAGCACGG CAGCATCGTC GAGCATTTCC GCGCGTCCGA ACTGGAAGGC AAGATGCCGA CGCTGCACGA CCTGCTCGGG GTG

Details for BuceA.00057.b.A1.GE33165

HARVESTED ON:	8/30/2011
SEQUENCED ON:	9/2/2011
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMKHSEREER ELSGVESGTP ALEITGLEAW YGESHILHGV DLTVHRGEVV TLLGRNGAGR TTTLRAIMGL TGRRSGSIKV AGHETIGLAT HRIAHYGIGY CPEERGIFSS LSCEENLMLP PLIGSREHAM SLDEIYAMFP NLASRRQSQG TRLSGGEQQM LAVARILRTG ANLLLLDEIS EGLAPVIVQA LARMIVALKA RGYTIVMVEQ NFRFAAPLAD RFYVMEHGSI VEHFRASELE XKMPTLHDLL GV

Validated NT Sequence

aattttnntt nnctttaagn aaggagatat accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatga agcacagcga acgggaggaa cgcgaattga gcggcgtcga gagcggtacg cctgcgctgg agatcacggg cctcgaggcc tggtacgggg aatcccacat cttgcacggt gtcgacctga cggtgcatcg cggcgaggtc gtcacgctgc tcggccgcaa cggcgcgggc cgcacgacga cgctgcgcgc gatcatgggc ctcacgggcc gccgcagcgg gtcgatcaag gtcgccggcc acgagacgat cggcctcgcg acgcaccgca tcgcgcatta cggcatcggt tactgcccgg aggagcgcgg gatcttctcg agcctgtcgt gcgaggagaa cctgatgctg ccgccgctga tcgggtcgcg cgaacacgcg atgtcgctcg acgagatcta cgcgatgttc ccgaacctcg cgtcgcgccg gcagagccag ggcacgcggc tgtccggtgg cgagcagcag atgctcgcgg tcgcgcggat cttgcgcacc ggcgcgaacc tgctgctgct cgacgagatt tccgagggcc tcgcgccggt gatcgtgcag gcgctcgcgc ggatgatcgt cgcgctgaag gcgcgcggct acacgatcgt gatggtcgaa cagaatttcc gcttcgccgc gccgctcgcc gaccgcttct acgtgatgga gcacggcagc atcgtcgagc atttccgcgc gtccgaactg gaangcaaga tgccgacgct gcacgacctg ctcggggtgt aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctganttg gctnctgnnc nnnnnnnnnn naaaaaaaaa aaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagcacagc gaacgggagg aacgcgaatt gagcggcgtc gagagcggta cgcctgcgct ggagatcacg ggcctcgagg cctggtacgg ggaatcccac atcttgcacg gtgtcgacct gacggtgcat cgcggcgagg tcgtcacgct gctcggccgc aacggcgcgg gccgcacgac gacgctgcgc gcgatcatgg gcctcacggg ccgccgcagc gggtcgatca aggtcgccgg ccacgagacg atcggcctcg cgacgcaccg catcgcgcat tacggcatcg gttactgccc ggaggagcgc gggatcttct cgagcctgtc gtgcgaggag aacctgatgc tgccgccgct gatcgggtcg cgcgaacacg cgatgtcgct cgacgagatc tacgcgatgt tcccgaacct cgcgtcgcgc cggcagagcc agggcacgcg gctgtccggt ggcgagcagc agatgctcgc ggtcgcgcgg atcttgcgca ccggcgcgaa cctgctgctg ctcgacgaga tttccgaggg cctcgcgccg gtgatcgtgc aggcgctcgc gcggatgatc gtcgcgctga aggcgcgcgg ctacacgatc gtgatggtcg aacagaattt ccgcttcgcc gcgccgctcg ccgaccgctt ctacgtgatg gagcacggca gcatcgtcga gcatttccgc gcgtccgaac tggaaggcaa gatgccgacg ctgcacgacc tgctcggggt gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa