BuceA.00057.a

Putative branched-chain amino acid ABC transporter ATP-binding protein

CENTER ID: BuceA.00057.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00057.a.A1.GE33164 Full length( BuceA.00057.a ) 1 259
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.18
RefSeq: YP_002229179.1
UniProt: B4E572

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MILGDTILET RGLTKEFRGF TAVNGVNLRV RRGAIHALIG PNGAGKTTCF NLLTKFLTPT AGQIVFNGID ITDERPAQVA RRGIIRSFQI SAVFPHLTAL QNVRIGLQRA LGTEFHFWRS ERTLKRLDDR AMDLLTQVGL TDFAHVPTVE LAYGRKRALE IATTLAMEPE LMLLDEPTQG MGHEDVDRVT ALIKKVASGR TILMVEHNMN VIAGISDTIT VLQRGEVLAE GSYAEVSKNP LVIEAYMGSA DAALAGAHA
NT Sequence
ATGATTCTCG GCGACACGAT TCTGGAAACA CGCGGACTGA CGAAGGAATT CAGGGGCTTC ACCGCCGTGA ACGGCGTCAA CCTGCGTGTG CGGCGCGGTG CGATCCACGC ATTGATCGGG CCGAACGGCG CGGGCAAGAC CACCTGTTTC AACCTCCTCA CCAAATTTCT GACGCCGACG GCCGGCCAGA TCGTCTTCAA CGGCATCGAC ATCACCGACG AGCGGCCCGC GCAGGTCGCG CGGCGCGGCA TCATCCGCTC GTTCCAGATC TCGGCCGTGT TCCCGCACCT CACCGCGCTG CAGAACGTGC GCATCGGCCT GCAACGCGCG CTCGGCACCG AATTTCATTT CTGGCGCAGC GAGCGCACGC TGAAGCGGCT CGACGATCGC GCGATGGACC TGCTCACGCA GGTCGGCCTC ACCGATTTCG CGCACGTGCC GACCGTCGAG CTGGCCTACG GCCGCAAGCG CGCGCTCGAG ATCGCGACGA CGCTCGCAAT GGAGCCCGAA CTGATGCTGC TCGACGAGCC GACGCAGGGG ATGGGCCATG AGGACGTCGA CCGCGTGACC GCGTTGATCA AGAAGGTCGC GAGCGGGCGC ACGATCCTGA TGGTGGAACA CAACATGAAC GTGATCGCGG GGATCTCCGA CACGATCACC GTCCTGCAAC GCGGCGAGGT GCTGGCCGAA GGCTCGTATG CGGAAGTGTC GAAGAATCCG CTCGTCATCG AGGCCTACAT GGGCAGCGCC GACGCGGCGC TCGCGGGGGC GCACGCA
Details for BuceA.00057.a.A1.GE33164
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 30kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMILGDTILE TRGLTKEFRG FTAVNGVNLR VRRGAIHALI GPNGAGKTTC FNLLTKFLTP TAGQIVFNGI DITDERPAQV ARRGIIRSFQ ISAVFPHLTA LQNVRIGLQR ALGTEFHFWR SERTLKRLDD RAMDLLTQVG LTDFAHVPTV ELAYGRKRAL EIATTLAMEP ELMLLDEPTX GMGHEDVDRV TALIKKVASG RTILMVEHNM NVIAGISDTI TVLQRGEVLA EGSYAEVSKN PLVIEAYMGS ADAALAGAHA
Validated NT Sequence
tnnttaactt taagaaggag anataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgattctcg gcgacacgat tctggaaaca cgcggactga cgaaggaatt caggggcttc accgccgtga acggcgtcaa cctgcgtgtg cggcgcggtg cgatccacgc attgatcggg ccgaacggcg cgggcaagac cacctgtttc aacctcctca ccaaatttct gacgccgacg gccggccaga tcgtcttcaa cggcatcgac atcaccgacg agcggcccgc gcaggtcgcg cggcgcggca tcatccgctc gttccagatc tcggccgtgt tcccgcacct caccgcgctg cagaacgtgc gcatcggcct gcaacgcgcg ctcggcaccg aatttcattt ctggcgcagc gagcgcacgc tgaagcggct cgacgatcgc gcgatggacc tgctcacgca ggtcggcctc accgatttcg cgcacgtgcc gaccgtcgag ctggcctacg gccgcaagcg cgcgctcgag atcgcgacga cgctcgcaat ggagcccgaa ctgatgctgc tcgacgagcc gacgcanggg atgggccatg aggacgtcga ccgcgtgacc gcgttgatca agaaggtcgc gagcgggcgc acgatcctga tggtggaaca caacatgaac gtgatcgcgg ggatctccga cacgatcacc gtcctgcaac gcggcgaggt gctggccgaa ggctcgtatg cggaagtgtc gaagaatccg ctcgtcatcg aggcctacat gggcagcgcc gacgcggcgc tngcgggggc gcacgcataa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccngctgc taacaaagcc cgaaaggaag ctganttngc tgctg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMILGDTILE TRGLTKEFRG FTAVNGVNLR VRRGAIHALI GPNGAGKTTC FNLLTKFLTP TAGQIVFNGI DITDERPAQV ARRGIIRSFQ ISAVFPHLTA LQNVRIGLQR ALGTEFHFWR SERTLKRLDD RAMDLLTQVG LTDFAHVPTV ELAYGRKRAL EIATTLAMEP ELMLLDEPTQ GMGHEDVDRV TALIKKVASG RTILMVEHNM NVIAGISDTI TVLQRGEVLA EGSYAEVSKN PLVIEAYMGS ADAALAGAHA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattctcggc gacacgattc tggaaacacg cggactgacg aaggaattca ggggcttcac cgccgtgaac ggcgtcaacc tgcgtgtgcg gcgcggtgcg atccacgcat tgatcgggcc gaacggcgcg ggcaagacca cctgtttcaa cctcctcacc aaatttctga cgccgacggc cggccagatc gtcttcaacg gcatcgacat caccgacgag cggcccgcgc aggtcgcgcg gcgcggcatc atccgctcgt tccagatctc ggccgtgttc ccgcacctca ccgcgctgca gaacgtgcgc atcggcctgc aacgcgcgct cggcaccgaa tttcatttct ggcgcagcga gcgcacgctg aagcggctcg acgatcgcgc gatggacctg ctcacgcagg tcggcctcac cgatttcgcg cacgtgccga ccgtcgagct ggcctacggc cgcaagcgcg cgctcgagat cgcgacgacg ctcgcaatgg agcccgaact gatgctgctc gacgagccga cgcaggggat gggccatgag gacgtcgacc gcgtgaccgc gttgatcaag aaggtcgcga gcgggcgcac gatcctgatg gtggaacaca acatgaacgt gatcgcgggg atctccgaca cgatcaccgt cctgcaacgc ggcgaggtgc tggccgaagg ctcgtatgcg gaagtgtcga agaatccgct cgtcatcgag gcctacatgg gcagcgccga cgcggcgctc gcgggggcgc acgcataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa