BuceA.00050.a
Putative epimerase
| CENTER ID: | BuceA.00050.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00050.a.B1.GE36642 | Full length( BuceA.00050.a ) | 1 | 321 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00050.a.B1.PS01829 | Full length( BuceA.00050.a ) | 1 | 321 | ||
| BuceA.00050.a.B1.PS01830 | Full length( BuceA.00050.a ) | 1 | 321 |
Structures
4LW8
DEPOSITED: 7/26/2013
DETERMINATION: XRay
CLONE: BuceA.00050.a.B1.GE36642
PROTEIN: BuceA.00050.a.B1.PS01829
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_209364 |
| PATRIC ID: | fig|216591.5.peg.4727 |
| RefSeq: | YP_002233621.1 |
| UniProt: | B4EFL1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRSETGRPS RRAFVTGLTG FTGRYMAERL QAAGYDVWGT VAPGTPRPAD PAFAQCTLLP VDLLDAEAMR AAAADARPDA VVHLAARAHV AQDEPSQTYA VNIVGTRNLL AALSGLDRRP SAVLLASSAN IYGNSTAGVL DETVAPAPAN DYAVSKLAME YAAKLWADRL PIVIARPFNY TGVGQSDAYL LPKLVAHYAR NAPRISLGNL DVSRDFSDVR DVTAAYLKLI EAAPAGETFN VCSERAYSLK EVLAMLSRIA GYVIDVTIDP RFVRHNEVKS LSGSRDKLRR AVGELPVTPL DETLRWMVDA MRAAPPGHAA G
NT Sequence
ATGACACGTT CTGAAACCGG GCGTCCGTCG CGCCGTGCAT TCGTCACGGG CCTGACGGGC TTCACCGGTC GCTACATGGC CGAACGCCTG CAGGCGGCCG GCTATGACGT CTGGGGCACG GTCGCGCCCG GCACGCCCCG CCCCGCCGAT CCGGCGTTCG CGCAGTGCAC GCTGCTGCCC GTCGACCTGC TCGACGCCGA GGCCATGCGC GCGGCTGCGG CCGATGCACG GCCCGACGCA GTCGTGCATC TCGCCGCGCG TGCGCATGTC GCACAGGACG AGCCGTCGCA GACCTACGCG GTCAACATCG TCGGCACGCG CAACCTGCTG GCTGCCCTCT CCGGCCTCGA CCGCCGTCCG TCGGCCGTGT TGCTGGCCAG CAGCGCGAAC ATCTACGGCA ACTCGACCGC CGGCGTGCTC GACGAAACCG TCGCGCCTGC GCCGGCGAAC GACTACGCGG TCAGCAAACT CGCGATGGAA TATGCGGCGA AGCTATGGGC CGACCGGCTG CCGATCGTGA TCGCCCGGCC GTTCAACTAC ACGGGCGTCG GCCAGAGCGA TGCCTATCTG CTGCCGAAGC TCGTCGCGCA CTATGCGCGC AACGCACCGC GGATCTCGCT CGGCAATCTC GACGTGAGCC GCGATTTCTC CGACGTGCGC GACGTGACGG CCGCCTATCT GAAGCTCATC GAAGCCGCAC CGGCAGGCGA GACGTTCAAC GTCTGCTCGG AGCGCGCGTA TTCACTGAAG GAAGTGCTGG CGATGCTGTC GCGCATCGCC GGTTACGTGA TCGACGTGAC GATCGATCCA CGCTTCGTGC GACACAACGA AGTGAAAAGC CTGAGCGGGT CGCGCGACAA GCTGCGGCGT GCGGTCGGCG AATTGCCGGT CACGCCGCTC GACGAAACGC TGCGATGGAT GGTGGACGCG ATGCGTGCTG CACCGCCCGG TCACGCCGCT GGC
Details for BuceA.00050.a.B1.GE36642
| HARVESTED ON: | 2/8/2013 |
| SEQUENCED ON: | 2/14/2013 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MTRSETGRPS RRAFVTGLTG FTGRYMAERL QAAGYDVWGT VAPGTPRPAD PAFAQCTLLP VDLLDAEAMR AAAADARPDA VVHLAARAHV AQDEPSQTYA VNIVGTRNLL AALSGLDRRP SAVLLASSAN IYGNSTAGVL DETVAPAPAN DYAVSKLAME YAAKLWADRL PIVIARPFNY TGVGQSDAYL LPKLVAHYAR NAPRISLGNL DVSRDFSDVR DVTAAYLKLX EAAPAGETFN VCSERAYSLK EVXXMLSRIA GYVIDVTIDP RFVRHNEXKS XXGSRDKLXR AVGEXPXTPL DETXRWMVDA MR
Validated NT Sequence
gcacgcatcg cgtccaccat ccatcgcanc gtttcgtcga gcggcgtgnc cggnaattcg ccgaccgcac gccncagctt gtcgcgcgac ccgntcangc ttttcncttc gttgtgtcgc acgaagcgtg gatcgatcgt cacgtcgatc acgtaaccgg cgatgcgcga cagcatcncc ngcacttcct tcagtgaata cgcgcgctcc gagcagacgt tgaacgtctc gcctgccggt gcggcttcna tgagcttcag ataggcggcc gtcacgtcnc gcacgtcnga gaaatcgcgg ctcacgtcga gattgccgag cgagatccgc ggtgcgttgc gcgcatagtg cgcgacgagc ttcggcagca gataggcatc gctctggccg acgcccgtgt agttgaacgg ccgggcgatc acgatcggca gccggtcggc ccatagcttc gccgcatatt ccatcgcgag tttgctgacc gcgtagtcgt tcgccggcgc aggcgcgacg gtttcgtcga gcacgccggc ggtcgagttg ccgtagatgt tcgcgctgct ggccagcaac acggccgacg gacggcggtc gaggccggag agggcagcca gcaggttgcg cgtgccgacg atgttgaccg cgtaggtctg cgacggctcg tcctgtgcga catgcgcacg cgcggcgaga tgcacgactg cgtcgggccg tgcatcggcc gcagccgcgc gcatggcctc ggcgtcgagc aggtcgacgg gcagcagcgt gcactgcgcg aacgccggat cggcggggcg gggcgtgccg ggcgcgaccg tgccccagac gtcatagccg gccgcctgca ggcgttcggc catgtagcga ccggtgaagc ccgtcaggcc cgtgacgaat gcacggcgcg acggacgccc ggtttcagaa cgtgtcatat ggtggtggtg gtggtgagc
Expected Protein Sequence
MAHHHHHHMT RSETGRPSRR AFVTGLTGFT GRYMAERLQA AGYDVWGTVA PGTPRPADPA FAQCTLLPVD LLDAEAMRAA AADARPDAVV HLAARAHVAQ DEPSQTYAVN IVGTRNLLAA LSGLDRRPSA VLLASSANIY GNSTAGVLDE TVAPAPANDY AVSKLAMEYA AKLWADRLPI VIARPFNYTG VGQSDAYLLP KLVAHYARNA PRISLGNLDV SRDFSDVRDV TAAYLKLIEA APAGETFNVC SERAYSLKEV LAMLSRIAGY VIDVTIDPRF VRHNEVKSLS GSRDKLRRAV GELPVTPLDE TLRWMVDAMR AAPPGHAAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac gttctgaaac cgggcgtccg tcgcgccgtg cattcgtcac gggcctgacg ggcttcaccg gtcgctacat ggccgaacgc ctgcaggcgg ccggctatga cgtctggggc acggtcgcgc ccggcacgcc ccgccccgcc gatccggcgt tcgcgcagtg cacgctgctg cccgtcgacc tgctcgacgc cgaggccatg cgcgcggctg cggccgatgc acggcccgac gcagtcgtgc atctcgccgc gcgtgcgcat gtcgcacagg acgagccgtc gcagacctac gcggtcaaca tcgtcggcac gcgcaacctg ctggctgccc tctccggcct cgaccgccgt ccgtcggccg tgttgctggc cagcagcgcg aacatctacg gcaactcgac cgccggcgtg ctcgacgaaa ccgtcgcgcc tgcgccggcg aacgactacg cggtcagcaa actcgcgatg gaatatgcgg cgaagctatg ggccgaccgg ctgccgatcg tgatcgcccg gccgttcaac tacacgggcg tcggccagag cgatgcctat ctgctgccga agctcgtcgc gcactatgcg cgcaacgcac cgcggatctc gctcggcaat ctcgacgtga gccgcgattt ctccgacgtg cgcgacgtga cggccgccta tctgaagctc atcgaagccg caccggcagg cgagacgttc aacgtctgct cggagcgcgc gtattcactg aaggaagtgc tggcgatgct gtcgcgcatc gccggttacg tgatcgacgt gacgatcgat ccacgcttcg tgcgacacaa cgaagtgaaa agcctgagcg ggtcgcgcga caagctgcgg cgtgcggtcg gcgaattgcc ggtcacgccg ctcgacgaaa cgctgcgatg gatggtggac gcgatgcgtg ctgcaccgcc cggtcacgcc gctggctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BuceA.00050.a.B1.PS01829
| PURIFICATION DATe: | 5/21/2013 |
| CONCENTRATION: | 69.3mg/ml |
| OBSERVED MW: | 34kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTRSETGRPS RRAFVTGLTG FTGRYMAERL QAAGYDVWGT VAPGTPRPAD PAFAQCTLLP VDLLDAEAMR AAAADARPDA VVHLAARAHV AQDEPSQTYA VNIVGTRNLL AALSGLDRRP SAVLLASSAN IYGNSTAGVL DETVAPAPAN DYAVSKLAME YAAKLWADRL PIVIARPFNY TGVGQSDAYL LPKLVAHYAR NAPRISLGNL DVSRDFSDVR DVTAAYLKLX EAAPAGETFN VCSERAYSLK EVXXMLSRIA GYVIDVTIDP RFVRHNEXKS XXGSRDKLXR AVGEXPXTPL DETXRWMVDA MR
Validated NT Sequence
gcacgcatcg cgtccaccat ccatcgcanc gtttcgtcga gcggcgtgnc cggnaattcg ccgaccgcac gccncagctt gtcgcgcgac ccgntcangc ttttcncttc gttgtgtcgc acgaagcgtg gatcgatcgt cacgtcgatc acgtaaccgg cgatgcgcga cagcatcncc ngcacttcct tcagtgaata cgcgcgctcc gagcagacgt tgaacgtctc gcctgccggt gcggcttcna tgagcttcag ataggcggcc gtcacgtcnc gcacgtcnga gaaatcgcgg ctcacgtcga gattgccgag cgagatccgc ggtgcgttgc gcgcatagtg cgcgacgagc ttcggcagca gataggcatc gctctggccg acgcccgtgt agttgaacgg ccgggcgatc acgatcggca gccggtcggc ccatagcttc gccgcatatt ccatcgcgag tttgctgacc gcgtagtcgt tcgccggcgc aggcgcgacg gtttcgtcga gcacgccggc ggtcgagttg ccgtagatgt tcgcgctgct ggccagcaac acggccgacg gacggcggtc gaggccggag agggcagcca gcaggttgcg cgtgccgacg atgttgaccg cgtaggtctg cgacggctcg tcctgtgcga catgcgcacg cgcggcgaga tgcacgactg cgtcgggccg tgcatcggcc gcagccgcgc gcatggcctc ggcgtcgagc aggtcgacgg gcagcagcgt gcactgcgcg aacgccggat cggcggggcg gggcgtgccg ggcgcgaccg tgccccagac gtcatagccg gccgcctgca ggcgttcggc catgtagcga ccggtgaagc ccgtcaggcc cgtgacgaat gcacggcgcg acggacgccc ggtttcagaa cgtgtcatat ggtggtggtg gtggtgagc
Expressed Protein Sequence
MAHHHHHHMT RSETGRPSRR AFVTGLTGFT GRYMAERLQA AGYDVWGTVA PGTPRPADPA FAQCTLLPVD LLDAEAMRAA AADARPDAVV HLAARAHVAQ DEPSQTYAVN IVGTRNLLAA LSGLDRRPSA VLLASSANIY GNSTAGVLDE TVAPAPANDY AVSKLAMEYA AKLWADRLPI VIARPFNYTG VGQSDAYLLP KLVAHYARNA PRISLGNLDV SRDFSDVRDV TAAYLKLIEA APAGETFNVC SERAYSLKEV LAMLSRIAGY VIDVTIDPRF VRHNEVKSLS GSRDKLRRAV GELPVTPLDE TLRWMVDAMR AAPPGHAAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAC GTTCTGAAAC CGGGCGTCCG TCGCGCCGTG CATTCGTCAC GGGCCTGACG GGCTTCACCG GTCGCTACAT GGCCGAACGC CTGCAGGCGG CCGGCTATGA CGTCTGGGGC ACGGTCGCGC CCGGCACGCC CCGCCCCGCC GATCCGGCGT TCGCGCAGTG CACGCTGCTG CCCGTCGACC TGCTCGACGC CGAGGCCATG CGCGCGGCTG CGGCCGATGC ACGGCCCGAC GCAGTCGTGC ATCTCGCCGC GCGTGCGCAT GTCGCACAGG ACGAGCCGTC GCAGACCTAC GCGGTCAACA TCGTCGGCAC GCGCAACCTG CTGGCTGCCC TCTCCGGCCT CGACCGCCGT CCGTCGGCCG TGTTGCTGGC CAGCAGCGCG AACATCTACG GCAACTCGAC CGCCGGCGTG CTCGACGAAA CCGTCGCGCC TGCGCCGGCG AACGACTACG CGGTCAGCAA ACTCGCGATG GAATATGCGG CGAAGCTATG GGCCGACCGG CTGCCGATCG TGATCGCCCG GCCGTTCAAC TACACGGGCG TCGGCCAGAG CGATGCCTAT CTGCTGCCGA AGCTCGTCGC GCACTATGCG CGCAACGCAC CGCGGATCTC GCTCGGCAAT CTCGACGTGA GCCGCGATTT CTCCGACGTG CGCGACGTGA CGGCCGCCTA TCTGAAGCTC ATCGAAGCCG CACCGGCAGG CGAGACGTTC AACGTCTGCT CGGAGCGCGC GTATTCACTG AAGGAAGTGC TGGCGATGCT GTCGCGCATC GCCGGTTACG TGATCGACGT GACGATCGAT CCACGCTTCG TGCGACACAA CGAAGTGAAA AGCCTGAGCG GGTCGCGCGA CAAGCTGCGG CGTGCGGTCG GCGAATTGCC GGTCACGCCG CTCGACGAAA CGCTGCGATG GATGGTGGAC GCGATGCGTG CTGCACCGCC CGGTCACGCC GCTGGCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for BuceA.00050.a.B1.PS01830
| PURIFICATION DATe: | 5/21/2013 |
| CONCENTRATION: | 71.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTRSETGRPS RRAFVTGLTG FTGRYMAERL QAAGYDVWGT VAPGTPRPAD PAFAQCTLLP VDLLDAEAMR AAAADARPDA VVHLAARAHV AQDEPSQTYA VNIVGTRNLL AALSGLDRRP SAVLLASSAN IYGNSTAGVL DETVAPAPAN DYAVSKLAME YAAKLWADRL PIVIARPFNY TGVGQSDAYL LPKLVAHYAR NAPRISLGNL DVSRDFSDVR DVTAAYLKLX EAAPAGETFN VCSERAYSLK EVXXMLSRIA GYVIDVTIDP RFVRHNEXKS XXGSRDKLXR AVGEXPXTPL DETXRWMVDA MR
Validated NT Sequence
gcacgcatcg cgtccaccat ccatcgcanc gtttcgtcga gcggcgtgnc cggnaattcg ccgaccgcac gccncagctt gtcgcgcgac ccgntcangc ttttcncttc gttgtgtcgc acgaagcgtg gatcgatcgt cacgtcgatc acgtaaccgg cgatgcgcga cagcatcncc ngcacttcct tcagtgaata cgcgcgctcc gagcagacgt tgaacgtctc gcctgccggt gcggcttcna tgagcttcag ataggcggcc gtcacgtcnc gcacgtcnga gaaatcgcgg ctcacgtcga gattgccgag cgagatccgc ggtgcgttgc gcgcatagtg cgcgacgagc ttcggcagca gataggcatc gctctggccg acgcccgtgt agttgaacgg ccgggcgatc acgatcggca gccggtcggc ccatagcttc gccgcatatt ccatcgcgag tttgctgacc gcgtagtcgt tcgccggcgc aggcgcgacg gtttcgtcga gcacgccggc ggtcgagttg ccgtagatgt tcgcgctgct ggccagcaac acggccgacg gacggcggtc gaggccggag agggcagcca gcaggttgcg cgtgccgacg atgttgaccg cgtaggtctg cgacggctcg tcctgtgcga catgcgcacg cgcggcgaga tgcacgactg cgtcgggccg tgcatcggcc gcagccgcgc gcatggcctc ggcgtcgagc aggtcgacgg gcagcagcgt gcactgcgcg aacgccggat cggcggggcg gggcgtgccg ggcgcgaccg tgccccagac gtcatagccg gccgcctgca ggcgttcggc catgtagcga ccggtgaagc ccgtcaggcc cgtgacgaat gcacggcgcg acggacgccc ggtttcagaa cgtgtcatat ggtggtggtg gtggtgagc
Expressed Protein Sequence
MAHHHHHHMT RSETGRPSRR AFVTGLTGFT GRYMAERLQA AGYDVWGTVA PGTPRPADPA FAQCTLLPVD LLDAEAMRAA AADARPDAVV HLAARAHVAQ DEPSQTYAVN IVGTRNLLAA LSGLDRRPSA VLLASSANIY GNSTAGVLDE TVAPAPANDY AVSKLAMEYA AKLWADRLPI VIARPFNYTG VGQSDAYLLP KLVAHYARNA PRISLGNLDV SRDFSDVRDV TAAYLKLIEA APAGETFNVC SERAYSLKEV LAMLSRIAGY VIDVTIDPRF VRHNEVKSLS GSRDKLRRAV GELPVTPLDE TLRWMVDAMR AAPPGHAAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAC GTTCTGAAAC CGGGCGTCCG TCGCGCCGTG CATTCGTCAC GGGCCTGACG GGCTTCACCG GTCGCTACAT GGCCGAACGC CTGCAGGCGG CCGGCTATGA CGTCTGGGGC ACGGTCGCGC CCGGCACGCC CCGCCCCGCC GATCCGGCGT TCGCGCAGTG CACGCTGCTG CCCGTCGACC TGCTCGACGC CGAGGCCATG CGCGCGGCTG CGGCCGATGC ACGGCCCGAC GCAGTCGTGC ATCTCGCCGC GCGTGCGCAT GTCGCACAGG ACGAGCCGTC GCAGACCTAC GCGGTCAACA TCGTCGGCAC GCGCAACCTG CTGGCTGCCC TCTCCGGCCT CGACCGCCGT CCGTCGGCCG TGTTGCTGGC CAGCAGCGCG AACATCTACG GCAACTCGAC CGCCGGCGTG CTCGACGAAA CCGTCGCGCC TGCGCCGGCG AACGACTACG CGGTCAGCAA ACTCGCGATG GAATATGCGG CGAAGCTATG GGCCGACCGG CTGCCGATCG TGATCGCCCG GCCGTTCAAC TACACGGGCG TCGGCCAGAG CGATGCCTAT CTGCTGCCGA AGCTCGTCGC GCACTATGCG CGCAACGCAC CGCGGATCTC GCTCGGCAAT CTCGACGTGA GCCGCGATTT CTCCGACGTG CGCGACGTGA CGGCCGCCTA TCTGAAGCTC ATCGAAGCCG CACCGGCAGG CGAGACGTTC AACGTCTGCT CGGAGCGCGC GTATTCACTG AAGGAAGTGC TGGCGATGCT GTCGCGCATC GCCGGTTACG TGATCGACGT GACGATCGAT CCACGCTTCG TGCGACACAA CGAAGTGAAA AGCCTGAGCG GGTCGCGCGA CAAGCTGCGG CGTGCGGTCG GCGAATTGCC GGTCACGCCG CTCGACGAAA CGCTGCGATG GATGGTGGAC GCGATGCGTG CTGCACCGCC CGGTCACGCC GCTGGCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T