BuceA.00047.a

Aspartate ammonia-lyase

CENTER ID: BuceA.00047.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00047.a.B1.GE36641 Full length( BuceA.00047.a ) 1 468
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.4918
RefSeq: YP_002233806.1
UniProt: B4EGU5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAENGFRVEA DLLGKRSIPD AAYYGVHTLR AKENFDISGR TIASLPYLVM ALAAVKEAAA DANCELGLLP RPYRDAIAAA CVEIREGRLH DQFVVDVIQG GAGTSTNMNA NEVICNRALE IMGHARGQYE YLHPNEHVNL AQSTNDVYPT AIRIATCFAV EHLLEAMARL RDAFAERADA FAGLLKLGRT QLQDAVPMTL GQEFSTYAVM LTEDIARLQE AGWLIREINL GATAIGTGIT AHPEYAEKAL AALRRITGLD LSTAPNLIEA TQDCGAFVQV SGVLKRIAVK LSKICNDLRL LSSGPRAGFG EINLPPVQAG SSIMPGKVNP VIPEVVNQVA FEVFGNDLTI TFAAEAGQLQ LNAFEPVIAS ALFRSFSHLT AACTTLAQRC VSGITANPER LRETMERSVA LATALNPYIG YKRATAVAAE AHATGKSIRE VVLARELMTA AQLDEALQPE ALIRPRAR
NT Sequence
ATGGCAGAAA ACGGCTTCCG CGTCGAAGCG GATCTTTTAG GGAAACGAAG TATTCCGGAT GCCGCCTACT ACGGCGTCCA CACGCTGCGC GCGAAAGAGA ATTTCGACAT TTCGGGCCGC ACCATTGCGT CGCTGCCGTA CCTCGTGATG GCGCTCGCGG CCGTGAAGGA AGCGGCGGCC GACGCCAACT GCGAACTCGG GCTGCTGCCG CGGCCGTACC GCGATGCGAT CGCCGCCGCG TGCGTCGAGA TCCGCGAAGG GCGGCTGCAC GACCAGTTCG TCGTCGACGT GATCCAGGGC GGCGCCGGCA CGTCGACCAA CATGAACGCG AACGAGGTGA TCTGCAACCG CGCGCTCGAA ATCATGGGAC ACGCGCGCGG GCAGTACGAA TACCTGCATC CGAACGAACA CGTCAATCTC GCGCAGAGCA CCAACGACGT CTATCCGACC GCGATCCGGA TCGCGACGTG CTTCGCGGTC GAGCATCTGC TCGAAGCGAT GGCGCGGCTG CGCGATGCGT TCGCGGAGCG CGCCGACGCG TTCGCCGGCT TGCTGAAGCT CGGCCGCACG CAGTTGCAGG ACGCGGTGCC GATGACGCTC GGCCAGGAGT TCTCGACCTA CGCGGTGATG CTGACGGAGG ACATCGCGCG GCTGCAGGAG GCCGGCTGGC TGATTCGCGA GATCAACCTC GGCGCGACCG CGATCGGCAC GGGCATCACC GCGCATCCGG AGTACGCGGA GAAGGCGCTG GCCGCGCTGC GGCGCATCAC CGGGCTCGAC CTGAGCACCG CGCCGAACCT GATCGAGGCG ACGCAGGATT GCGGCGCGTT CGTCCAGGTG TCGGGCGTGC TCAAGCGGAT CGCCGTCAAG CTGTCGAAGA TCTGCAACGA CCTGCGACTG CTGTCGAGCG GCCCGCGCGC GGGCTTCGGC GAGATCAACC TGCCGCCGGT GCAGGCCGGT TCGTCGATCA TGCCCGGCAA GGTGAATCCG GTGATCCCGG AAGTCGTCAA CCAGGTCGCG TTCGAGGTGT TCGGCAACGA CCTGACCATC ACCTTCGCGG CCGAAGCCGG CCAGCTTCAG CTCAACGCGT TCGAACCGGT GATCGCGAGC GCGCTGTTCC GCAGCTTCAG CCACCTGACG GCCGCCTGCA CGACGCTCGC GCAGCGCTGC GTGAGCGGCA TCACCGCGAA CCCCGAGCGG CTGCGCGAAA CGATGGAGCG CTCGGTCGCG CTCGCGACCG CGCTGAATCC GTACATCGGC TACAAGCGCG CGACCGCCGT GGCCGCCGAA GCGCATGCGA CCGGCAAATC GATCCGCGAG GTCGTGCTGG CCCGCGAGCT GATGACCGCT GCGCAACTCG ACGAAGCGCT GCAACCGGAA GCGCTGATCC GGCCTCGGGC GCGC
Details for BuceA.00047.a.B1.GE36641
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 22
PERCENT COVERAGE: 88
Validated AA Sequence
MRFGGHGGRA LVADVRIQRG RERDRALHRF AQPLGVRGDA AHAALRERRA GGRQVAEAAE QRARDHRFER VELKLAGFGR EGDGQVVAEH LXRDLVDDFR DHRIHLAGHD RRTGLHRRQV DLAEARARAA RQQSQVVADL RQLDGDPLEH ARHLDERAAI LRRLDQVXRG AQVEPGDAPQ RGQRLLRVLR MRGDARADRG RAEVDLANQP AGLLQPRDVL RQHHRVGREL LAERHRHRVL QLRAAELQQA GERVGALRER IAQPRHRFEQ MLDREARRDP DRGRIDVVGA LREIDVFVRM QVFVLPARVS HDFERAVADH LVRVHVGRRA GAALDHVDDE LVVQPPFADL DARGGDRIAV RPRQQPEFAV GVGRRFLHGR ERHHEVRQRR NGAARNVEIL FRAQRVDAVV GGIRNTSFP
Validated NT Sequence
ttccggttgc agcgcttcgt cgagttgcgc agcggtcatc agctcgcggg ccagcacgac ctcgcggatc gatttgccgg tcgcatgcgc ttcggcggcc acggcggtcg cgcgcttgta gccgatgtac ggattcagcg cggtcgcgag cgcgaccgag cgctccatcg tttcgcgcag ccgctcgggg ttcgcggtga tgccgctcac gcagcgctgc gcgagcgtcg tgcaggcggc cgtcaggtgg ctgaagctgc ggaacagcgc gctcgcgatc accggttcga acgcgttgag ctgaagctgg ccggcttcgg ccgcgaaggt gatggtcagg tcgttgccga acacctcnaa cgcgacctgg ttgacgactt ccgggatcac cggattcacc ttgccgggca tgatcgacga accggcctgc accggcggca ggttgatctc gccgaagccc gcgcgcgggc cgctcgacag cagtcgcagg tcgttgcaga tcttcgacag cttgacggcg atccgcttga gcacgcccga cacctggacg aacgcgccgc aatcctgcgt cgcctcgatc aggttnggcg cggtgctcag gtcgagcccg gtgatgcgcc gcagcgcggc cagcgccttc tccgcgtact ccggatgcgc ggtgatgccc gtgccgatcg cggtcgcgcc gaggttgatc tcgcgaatca gccagccggc ctcctgcagc cgcgcgatgt cctccgtcag catcaccgcg taggtcgaga actcctggcc gagcgtcatc ggcaccgcgt cctgcaactg cgtgcggccg agcttcagca agccggcgaa cgcgtcggcg cgctccgcga acgcatcgcg cagccgcgcc atcgcttcga gcagatgctc gaccgcgaag cacgtcgcga tccggatcgc ggtcggatag acgtcgttgg tgctctgcgc gagattgacg tgttcgttcg gatgcaggta ttcgtactgc ccgcgcgcgt gtcccatgat ttcgagcgcg cggttgcaga tcacctcgtt cgcgttcatg ttggtcgacg tgccggcgcc gccctggatc acgtcgacga cgaactggtc gtgcagccgc ccttcgcgga tctcgacgca cgcggcggcg atcgcatcgc ggtacggccg cggcagcagc ccgagttcgc agttggcgtc ggccgccgct tccttcacgg ccgcgagcgc catcacgagg tacggcagcg acgcaatggt gcggcccgaa atgtcgaaat tctctttcgc gcgcagcgtg tggacgccgt agtaggcggc atccggaata cttcgtttcc ctaaaagatc cgcttcgacg cggaagccgt t
Expected Protein Sequence
MAHHHHHHMA ENGFRVEADL LGKRSIPDAA YYGVHTLRAK ENFDISGRTI ASLPYLVMAL AAVKEAAADA NCELGLLPRP YRDAIAAACV EIREGRLHDQ FVVDVIQGGA GTSTNMNANE VICNRALEIM GHARGQYEYL HPNEHVNLAQ STNDVYPTAI RIATCFAVEH LLEAMARLRD AFAERADAFA GLLKLGRTQL QDAVPMTLGQ EFSTYAVMLT EDIARLQEAG WLIREINLGA TAIGTGITAH PEYAEKALAA LRRITGLDLS TAPNLIEATQ DCGAFVQVSG VLKRIAVKLS KICNDLRLLS SGPRAGFGEI NLPPVQAGSS IMPGKVNPVI PEVVNQVAFE VFGNDLTITF AAEAGQLQLN AFEPVIASAL FRSFSHLTAA CTTLAQRCVS GITANPERLR ETMERSVALA TALNPYIGYK RATAVAAEAH ATGKSIREVV LARELMTAAQ LDEALQPEAL IRPRAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag aaaacggctt ccgcgtcgaa gcggatcttt tagggaaacg aagtattccg gatgccgcct actacggcgt ccacacgctg cgcgcgaaag agaatttcga catttcgggc cgcaccattg cgtcgctgcc gtacctcgtg atggcgctcg cggccgtgaa ggaagcggcg gccgacgcca actgcgaact cgggctgctg ccgcggccgt accgcgatgc gatcgccgcc gcgtgcgtcg agatccgcga agggcggctg cacgaccagt tcgtcgtcga cgtgatccag ggcggcgccg gcacgtcgac caacatgaac gcgaacgagg tgatctgcaa ccgcgcgctc gaaatcatgg gacacgcgcg cgggcagtac gaatacctgc atccgaacga acacgtcaat ctcgcgcaga gcaccaacga cgtctatccg accgcgatcc ggatcgcgac gtgcttcgcg gtcgagcatc tgctcgaagc gatggcgcgg ctgcgcgatg cgttcgcgga gcgcgccgac gcgttcgccg gcttgctgaa gctcggccgc acgcagttgc aggacgcggt gccgatgacg ctcggccagg agttctcgac ctacgcggtg atgctgacgg aggacatcgc gcggctgcag gaggccggct ggctgattcg cgagatcaac ctcggcgcga ccgcgatcgg cacgggcatc accgcgcatc cggagtacgc ggagaaggcg ctggccgcgc tgcggcgcat caccgggctc gacctgagca ccgcgccgaa cctgatcgag gcgacgcagg attgcggcgc gttcgtccag gtgtcgggcg tgctcaagcg gatcgccgtc aagctgtcga agatctgcaa cgacctgcga ctgctgtcga gcggcccgcg cgcgggcttc ggcgagatca acctgccgcc ggtgcaggcc ggttcgtcga tcatgcccgg caaggtgaat ccggtgatcc cggaagtcgt caaccaggtc gcgttcgagg tgttcggcaa cgacctgacc atcaccttcg cggccgaagc cggccagctt cagctcaacg cgttcgaacc ggtgatcgcg agcgcgctgt tccgcagctt cagccacctg acggccgcct gcacgacgct cgcgcagcgc tgcgtgagcg gcatcaccgc gaaccccgag cggctgcgcg aaacgatgga gcgctcggtc gcgctcgcga ccgcgctgaa tccgtacatc ggctacaagc gcgcgaccgc cgtggccgcc gaagcgcatg cgaccggcaa atcgatccgc gaggtcgtgc tggcccgcga gctgatgacc gctgcgcaac tcgacgaagc gctgcaaccg gaagcgctga tccggcctcg ggcgcgctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at