BuceA.00046.c

Alpha,alpha-trehalose-phosphate synthase

CENTER ID: BuceA.00046.c
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00046.c.B1.GE36640 Full length( BuceA.00046.c ) 1 468
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.5335
RefSeq: YP_002234185.1
UniProt: B4EK36

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRLVVVSNR IADPRKAAAG GLAVAVKDSL QETGGVWFGW SGRLRGGDDQ AARGDDVQIQ NVGGIQLATI DLDPQDYDAY YLGYSNNVLW PVFHYRLDLA QFDRRFADGY RRVNQLFARK LSTLLRPDDV IWVHDYQLIP LAAELRAMGC TNPIGFFLHI PMPPPPIMAA IPEHEWLMRS LFAYDLVGFQ TESDLLHFEH YVEAEAGAAR LPDGRLRAFG RTLSAGAFPI GINVDEFESL ARDRDGLDMF ERMRDEYSRR QLLVGVDRLD YTKGLPQRVH AFRQLLEQYP ENRDRATLIQ IAAPSREDLG AYDDLRREMD SLCGAINGDY GELEWMPMRY IHRTVARKRL PGLYRASRVA LVTPLRDGMN LVAKEFLAAQ DAADPGVLVL SRFAGAAEQL KSALLVNPYD TQGTAQAIQR ALTMPLEERR QRHAALMAIM RKTDVHWWRT RFLEALTEAA EVAAAAES
NT Sequence
GTGAGCCGAC TCGTCGTCGT ATCCAATCGC ATCGCAGATC CCCGTAAAGC CGCGGCCGGC GGCCTCGCCG TCGCCGTGAA GGACAGCCTG CAGGAAACCG GCGGCGTCTG GTTCGGCTGG AGCGGCAGGC TACGCGGCGG CGACGATCAG GCCGCGCGGG GCGACGACGT GCAGATCCAG AACGTCGGCG GCATCCAGCT CGCGACGATC GATCTCGATC CGCAGGATTA CGACGCTTAC TACCTCGGCT ACTCGAACAA CGTGCTGTGG CCGGTGTTTC ACTATCGGCT CGACCTCGCG CAGTTCGATC GACGCTTCGC CGACGGTTAC CGGCGCGTGA ACCAGCTGTT CGCGCGCAAG CTGAGCACGC TGCTGCGGCC CGACGACGTC ATCTGGGTGC ACGACTACCA GCTGATTCCG CTCGCCGCCG AGCTGCGCGC GATGGGGTGC ACGAATCCGA TCGGCTTCTT CCTGCACATC CCGATGCCGC CGCCGCCGAT CATGGCCGCG ATCCCGGAAC ACGAATGGCT GATGCGCTCG CTGTTCGCGT ATGACCTCGT CGGCTTCCAG ACCGAATCGG ATCTGCTGCA CTTCGAGCAT TACGTCGAGG CGGAAGCCGG CGCGGCGCGG TTGCCGGACG GCCGGCTGCG CGCGTTCGGC CGCACGCTGT CGGCCGGCGC GTTCCCGATC GGCATCAACG TCGACGAGTT CGAGTCGCTC GCGCGCGATC GCGACGGCCT CGACATGTTC GAGCGGATGC GCGACGAATA TTCGCGCCGC CAGTTGCTGG TCGGCGTCGA CCGGCTCGAT TACACGAAAG GGCTGCCGCA GCGCGTGCAT GCGTTCCGCC AGCTGCTCGA ACAGTATCCG GAGAACCGCG ACCGCGCGAC GCTGATCCAG ATCGCCGCGC CGAGCCGCGA GGATCTCGGC GCGTACGACG ACCTGCGGCG CGAGATGGAC AGCCTGTGCG GCGCGATCAA CGGCGACTAC GGCGAGCTCG AATGGATGCC GATGCGCTAC ATCCACCGCA CCGTCGCGCG CAAGCGCCTG CCGGGCCTCT ACCGCGCAAG CCGGGTCGCG CTCGTCACGC CGCTGCGCGA CGGGATGAAC CTCGTCGCGA AAGAGTTCCT CGCCGCGCAG GACGCGGCCG ACCCCGGCGT GCTCGTGCTG TCGCGTTTCG CGGGCGCGGC CGAGCAACTG AAGTCGGCGC TGCTCGTCAA TCCGTACGAC ACGCAGGGCA CCGCGCAGGC GATCCAGCGC GCGCTCACGA TGCCGCTCGA AGAGCGCCGC CAGCGTCACG CGGCGCTGAT GGCGATCATG CGCAAGACCG ACGTGCACTG GTGGCGCACG CGCTTTCTCG AAGCGCTCAC CGAAGCGGCC GAAGTGGCGG CCGCGGCCGA ATCC
Details for BuceA.00046.c.B1.GE36640
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 25
PERCENT COVERAGE: 49
Validated AA Sequence
MHALRQPFRV IEPVDADQQL AARIFVAHPL EHVEAVAIAR ERLELVDVDA DRERAGRQRA AERAQPAVRQ PRRAGFRLDV MLEVQQIRFG LEADEVIREQ RAHQPFVFRD RGHDRRRRHR DVQEEADRIR APHRAQLGGE RNQLVVVHPD DVVGPQQRAQ LAREQLVHAP VTVGEASIEL REVEPIVKHR PQHVVRVAEV VSVVILRIEI DRRELDAADV LDLHVVAPRG LIVAAA
Validated NT Sequence
ctgcgcggcg aggaactctt tcgcgacgag gttcatcccg tcgcgcagcg gcgtgacgag cgcgacccgg cttgcgcggt agaggcccgg caggcgcttg cgcgcgacgg tgcggtggat gtagcgcatc ggcatccatt cgagctcgcc gtagtcgccg ttgatcgcgc cgcacaggct gtccatctcg cgccgcaggt cgtcgtacgc gccgagatcc tcgcggctcg gcgcggcgat ctggatcagc gtcgcgcggt cgcggttctc cggatactgt tcgagcagct ggcggaacgc atgcacgcgc tgcggcagcc ctttcgtgta atcgagccgg tcgacgccga ccagcaactg gcggcgcgaa tattcgtcgc gcatccgctc gaacatgtcg aggccgtcgc gatcgcgcgc gagcgactcg aactcgtcga cgttgatgcc gatcgggaac gcgccggccg acagcgtgcg gccgaacgcg cgcagccggc cgtccggcaa ccgcgccgcg ccggcttccg cctcgacgta atgctcgaag tgcagcagat ccgattcggt ctggaagccg acgaggtcat acgcgaacag cgagcgcatc agccattcgt gttccgggat cgcggccatg atcggcggcg gcggcatcgg gatgtgcagg aagaagccga tcggattcgt gcaccccatc gcgcgcagct cggcggcgag cggaatcagc tggtagtcgt gcacccagat gacgtcgtcg ggccgcagca gcgtgctcag cttgcgcgcg aacagctggt tcacgcgccg gtaaccgtcg gcgaagcgtc gatcgaactg cgcgaggtcg agccgatagt gaaacaccgg ccacagcacg ttgttcgagt agccgaggta gtaagcgtcg taatcctgcg gatcgagatc gatcgtcgcg agctggatgc cgccgacgtt ctggatctgc acgtcgtcgc cccgcgcggc ctgatcgtcg ccgccgcgta gcctgccgct ccagccgaac cagacgccgc cggtttcctg caggctgtcc ttcacggcga cggcgaggcc gccggccgcg gctttacggg gatctgcgat gcgattggat acgacgacga gtcg
Expected Protein Sequence
MAHHHHHHMV SRLVVVSNRI ADPRKAAAGG LAVAVKDSLQ ETGGVWFGWS GRLRGGDDQA ARGDDVQIQN VGGIQLATID LDPQDYDAYY LGYSNNVLWP VFHYRLDLAQ FDRRFADGYR RVNQLFARKL STLLRPDDVI WVHDYQLIPL AAELRAMGCT NPIGFFLHIP MPPPPIMAAI PEHEWLMRSL FAYDLVGFQT ESDLLHFEHY VEAEAGAARL PDGRLRAFGR TLSAGAFPIG INVDEFESLA RDRDGLDMFE RMRDEYSRRQ LLVGVDRLDY TKGLPQRVHA FRQLLEQYPE NRDRATLIQI AAPSREDLGA YDDLRREMDS LCGAINGDYG ELEWMPMRYI HRTVARKRLP GLYRASRVAL VTPLRDGMNL VAKEFLAAQD AADPGVLVLS RFAGAAEQLK SALLVNPYDT QGTAQAIQRA LTMPLEERRQ RHAALMAIMR KTDVHWWRTR FLEALTEAAE VAAAAES
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga gccgactcgt cgtcgtatcc aatcgcatcg cagatccccg taaagccgcg gccggcggcc tcgccgtcgc cgtgaaggac agcctgcagg aaaccggcgg cgtctggttc ggctggagcg gcaggctacg cggcggcgac gatcaggccg cgcggggcga cgacgtgcag atccagaacg tcggcggcat ccagctcgcg acgatcgatc tcgatccgca ggattacgac gcttactacc tcggctactc gaacaacgtg ctgtggccgg tgtttcacta tcggctcgac ctcgcgcagt tcgatcgacg cttcgccgac ggttaccggc gcgtgaacca gctgttcgcg cgcaagctga gcacgctgct gcggcccgac gacgtcatct gggtgcacga ctaccagctg attccgctcg ccgccgagct gcgcgcgatg gggtgcacga atccgatcgg cttcttcctg cacatcccga tgccgccgcc gccgatcatg gccgcgatcc cggaacacga atggctgatg cgctcgctgt tcgcgtatga cctcgtcggc ttccagaccg aatcggatct gctgcacttc gagcattacg tcgaggcgga agccggcgcg gcgcggttgc cggacggccg gctgcgcgcg ttcggccgca cgctgtcggc cggcgcgttc ccgatcggca tcaacgtcga cgagttcgag tcgctcgcgc gcgatcgcga cggcctcgac atgttcgagc ggatgcgcga cgaatattcg cgccgccagt tgctggtcgg cgtcgaccgg ctcgattaca cgaaagggct gccgcagcgc gtgcatgcgt tccgccagct gctcgaacag tatccggaga accgcgaccg cgcgacgctg atccagatcg ccgcgccgag ccgcgaggat ctcggcgcgt acgacgacct gcggcgcgag atggacagcc tgtgcggcgc gatcaacggc gactacggcg agctcgaatg gatgccgatg cgctacatcc accgcaccgt cgcgcgcaag cgcctgccgg gcctctaccg cgcaagccgg gtcgcgctcg tcacgccgct gcgcgacggg atgaacctcg tcgcgaaaga gttcctcgcc gcgcaggacg cggccgaccc cggcgtgctc gtgctgtcgc gtttcgcggg cgcggccgag caactgaagt cggcgctgct cgtcaatccg tacgacacgc agggcaccgc gcaggcgatc cagcgcgcgc tcacgatgcc gctcgaagag cgccgccagc gtcacgcggc gctgatggcg atcatgcgca agaccgacgt gcactggtgg cgcacgcgct ttctcgaagc gctcaccgaa gcggccgaag tggcggccgc ggccgaatcc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat