BuceA.00046.b

Alpha,alpha-trehalose-phosphate synthase

CENTER ID: BuceA.00046.b
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00046.b.B1.GE36639 Full length( BuceA.00046.b ) 1 461
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1084
RefSeq: YP_002230184.1
UniProt: B4ECJ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRLIIVSNR VAPISEGEPA AGGLAIGVYD ALKETGGMWF GWSGEVVASG APQPQIRIEE RGPVTFATVG LSRRDYDQYY RGFSNATLWP AFHYRADLIQ YDRHEFDGYR RVNVWLAQQL VPLLQDDDVI WVHDYHLIPF ARALRDAGVK NRIGFFLHIP FPAAQVLVNV PPHRELVESL CAFDLLGFQT EPDLRAFCDY VEFEADGDVE RDGHTVHVRA FGQTLRAAAY PIGVYPDEIA SLAQAGEHGK AVRTLATSLR GRQLIMSVDR LDYSKGLVER FRAFEKLLEH QASIRNRVSF LQIAPSTRAD LRAYQDIRLQ LEAESGRING RYAELDWAPI LYIHRQYDRQ VLAALYRLAR VGFVTPLRDG MNLVAKEYVS AQNPDDPGVL VLSRFAGAAR ELTGALIVNP IDIDGMADAL SQALTMPLAE RRARYADMIA QLRENNVSVW RDNFLRDLQH V
NT Sequence
ATGAGCCGGC TCATCATCGT TTCAAACCGC GTCGCGCCGA TTTCGGAAGG CGAACCGGCG GCGGGCGGCC TCGCGATCGG CGTCTATGAC GCGCTGAAGG AAACCGGCGG CATGTGGTTC GGCTGGAGCG GCGAAGTGGT CGCGTCCGGC GCGCCGCAGC CGCAGATCCG CATCGAGGAG CGCGGGCCCG TGACGTTCGC GACCGTCGGG CTGTCGCGTC GCGATTACGA CCAGTATTAC CGCGGGTTCT CGAACGCGAC GCTGTGGCCC GCGTTCCATT ACCGCGCGGA CCTGATCCAG TACGATCGCC ACGAGTTCGA CGGTTATCGC CGCGTCAACG TCTGGCTCGC GCAGCAGCTC GTGCCGCTGC TGCAGGACGA CGACGTGATC TGGGTGCACG ACTATCACCT GATTCCGTTC GCGCGCGCGC TGCGCGACGC CGGTGTGAAG AACCGCATCG GCTTCTTCCT GCACATTCCG TTTCCGGCCG CGCAGGTGCT CGTCAACGTA CCGCCGCACC GCGAACTCGT CGAGTCGCTG TGCGCGTTCG ATCTGCTCGG TTTCCAGACC GAGCCCGATC TGCGCGCGTT CTGCGACTAC GTCGAGTTCG AGGCGGACGG CGACGTCGAG CGCGACGGGC ACACGGTGCA CGTCCGGGCG TTCGGCCAGA CGCTGCGCGC GGCCGCCTAT CCGATCGGCG TATATCCGGA CGAGATTGCG TCGCTCGCGC AGGCGGGCGA ACACGGCAAG GCGGTGCGCA CGCTCGCGAC GTCGCTGCGC GGGCGGCAAC TGATCATGAG CGTCGACCGG CTCGATTATT CGAAGGGGCT CGTCGAGCGC TTCCGTGCCT TCGAGAAGCT GCTCGAACAC CAGGCGTCGA TCCGCAACCG CGTGTCGTTC CTGCAGATCG CGCCGTCGAC GCGCGCGGAT TTGCGTGCGT ACCAGGACAT CCGCCTGCAG CTCGAAGCGG AGTCCGGGCG CATCAACGGG CGCTACGCGG AGCTCGACTG GGCGCCGATC CTCTACATTC ACCGTCAGTA CGACCGCCAG GTGCTCGCCG CGCTGTACCG CCTTGCGCGT GTGGGCTTCG TGACGCCGCT GCGCGACGGG ATGAACCTCG TCGCGAAGGA GTACGTGTCC GCGCAGAATC CGGACGATCC GGGCGTGCTG GTGCTGTCGC GCTTCGCGGG TGCCGCGCGC GAGCTCACGG GCGCGCTGAT CGTCAATCCG ATCGATATCG ACGGGATGGC CGATGCGCTG TCGCAGGCGT TGACGATGCC GCTCGCCGAG CGGCGGGCGC GTTACGCGGA CATGATCGCG CAGCTTCGCG AGAACAACGT GTCGGTGTGG CGCGACAATT TCCTGCGCGA TCTGCAGCAC GTC
Details for BuceA.00046.b.B1.GE36639
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 53kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 88
Validated AA Sequence
MWFGWSGEVV ASGAPQPQIR IEERGPVTFA TVGLSRRDYD QYYRGFSNAT LWPAFHYRAD LIQYDRHEFD GYRRVNVWLA QQLVPLLQDD DVIWVHDYHL IPFARALRDA GVKNRIGFFL HIPFPAAQVL VNVPPHRELV ESLCAFDLLG FQTEPDLRAF CDYVEFEADG DVERDGHTVH VRAFGQTLRA AAYPIGVYPD EIASLAQAGE HGKAVRTLAT SLRGRQLIMS VDRLDYSKGL VERFRAFEKL LEHQASIRNR VSFLQIAPST RADLRAYQDI RLQLEAESGR INGRYAELDW APILYIHRQY DRQVLAALYR LARVGFVTPL RDGMNLVAKE YVSAQNPDDP GVLVLSRFAG AARELTGALI VNPIDIDGMA DALSQALTMP LAERRARYAD MIAQLRENNV SVWRD
Validated NT Sequence
attgtcgcgc cacaccgaca cgttgttctc gcgaagctgc gcgatcatgt ccgcgtaacg cgcccgccgc tcggcgagcg gcatcgtcaa cgcctgcgac agcgcatcgg ccatcccgtc gatatcgatc ggattgacga tcagcgcgcc cgtgagctcg cgcgcggcac ccgcgaagcg cgacagcacc agcacgcccg gatcgtccgg attctgcgcg gacacgtact ccttcgcgac gaggttcatc ccgtcgcgca gcggcgtcac gaagcccaca cgcgcaaggc ggtacagcgc ggcgagcacc tggcggtcgt actgacggtg aatgtagagg atcggcgccc agtcgagctc cgcgtagcgc ccgttgatgc gcccggactc cgcttcgagc tgcaggcgga tgtcctggta cgcacgcaaa tccgcgcgcg tcgacggcgc gatctgcagg aacgacacgc ggttgcggat cgacgcctgg tgttcgagca gcttctcgaa ggcacggaag cgctcgacga gccccttcga ataatcgagc cggtcgacgc tcatgatcag ttgccgcccg cgcagcgacg tcgcgagcgt gcgcaccgcc ttgccgtgtt cgcccgcctg cgcgagcgac gcaatctcgt ccggatatac gccgatcgga taggcggccg cgcgcagcgt ctggccgaac gcccggacgt gcaccgtgtg cccgtcgcgc tcgacgtcgc cgtccgcctc gaactcgacg tagtcgcaga acgcgcgcag atcgggctcg gtctggaaac cgagcagatc gaacgcgcac agcgactcga cgagttcgcg gtgcggcggt acgttgacga gcacctgcgc ggccggaaac ggaatgtgca ggaagaagcc gatgcggttc ttcacaccgg cgtcgcgcag cgcgcgcgcg aacggaatca ggtgatagtc gtgcacccag atcacgtcgt cgtcctgcag cagcggcacg agctgctgcg cgagccagac gttgacgcgg cgataaccgt cgaactcgtg gcgatcgtac tggatcaggt ccgcgcggta atggaacgcg ggccacagcg tcgcgttcga gaacccgcgg taatactggt cgtaatcgcg acgcgacagc ccgacggtcg cgaacgtcac gggcccgcgc tcctcgatgc ggatctgcgg ctgcggcgcg ccggacgcga ccacttcgcc gctccagccg aaccacatgc cgccggtttc cttcagcgcg tcatagacgc cgatcgcgag gccgcccgcc gccggttcgc cttccgaaat cggcgcgacg cggtttgaaa cgatgatga
Expected Protein Sequence
MAHHHHHHMS RLIIVSNRVA PISEGEPAAG GLAIGVYDAL KETGGMWFGW SGEVVASGAP QPQIRIEERG PVTFATVGLS RRDYDQYYRG FSNATLWPAF HYRADLIQYD RHEFDGYRRV NVWLAQQLVP LLQDDDVIWV HDYHLIPFAR ALRDAGVKNR IGFFLHIPFP AAQVLVNVPP HRELVESLCA FDLLGFQTEP DLRAFCDYVE FEADGDVERD GHTVHVRAFG QTLRAAAYPI GVYPDEIASL AQAGEHGKAV RTLATSLRGR QLIMSVDRLD YSKGLVERFR AFEKLLEHQA SIRNRVSFLQ IAPSTRADLR AYQDIRLQLE AESGRINGRY AELDWAPILY IHRQYDRQVL AALYRLARVG FVTPLRDGMN LVAKEYVSAQ NPDDPGVLVL SRFAGAAREL TGALIVNPID IDGMADALSQ ALTMPLAERR ARYADMIAQL RENNVSVWRD NFLRDLQHV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc ggctcatcat cgtttcaaac cgcgtcgcgc cgatttcgga aggcgaaccg gcggcgggcg gcctcgcgat cggcgtctat gacgcgctga aggaaaccgg cggcatgtgg ttcggctgga gcggcgaagt ggtcgcgtcc ggcgcgccgc agccgcagat ccgcatcgag gagcgcgggc ccgtgacgtt cgcgaccgtc gggctgtcgc gtcgcgatta cgaccagtat taccgcgggt tctcgaacgc gacgctgtgg cccgcgttcc attaccgcgc ggacctgatc cagtacgatc gccacgagtt cgacggttat cgccgcgtca acgtctggct cgcgcagcag ctcgtgccgc tgctgcagga cgacgacgtg atctgggtgc acgactatca cctgattccg ttcgcgcgcg cgctgcgcga cgccggtgtg aagaaccgca tcggcttctt cctgcacatt ccgtttccgg ccgcgcaggt gctcgtcaac gtaccgccgc accgcgaact cgtcgagtcg ctgtgcgcgt tcgatctgct cggtttccag accgagcccg atctgcgcgc gttctgcgac tacgtcgagt tcgaggcgga cggcgacgtc gagcgcgacg ggcacacggt gcacgtccgg gcgttcggcc agacgctgcg cgcggccgcc tatccgatcg gcgtatatcc ggacgagatt gcgtcgctcg cgcaggcggg cgaacacggc aaggcggtgc gcacgctcgc gacgtcgctg cgcgggcggc aactgatcat gagcgtcgac cggctcgatt attcgaaggg gctcgtcgag cgcttccgtg ccttcgagaa gctgctcgaa caccaggcgt cgatccgcaa ccgcgtgtcg ttcctgcaga tcgcgccgtc gacgcgcgcg gatttgcgtg cgtaccagga catccgcctg cagctcgaag cggagtccgg gcgcatcaac gggcgctacg cggagctcga ctgggcgccg atcctctaca ttcaccgtca gtacgaccgc caggtgctcg ccgcgctgta ccgccttgcg cgtgtgggct tcgtgacgcc gctgcgcgac gggatgaacc tcgtcgcgaa ggagtacgtg tccgcgcaga atccggacga tccgggcgtg ctggtgctgt cgcgcttcgc gggtgccgcg cgcgagctca cgggcgcgct gatcgtcaat ccgatcgata tcgacgggat ggccgatgcg ctgtcgcagg cgttgacgat gccgctcgcc gagcggcggg cgcgttacgc ggacatgatc gcgcagcttc gcgagaacaa cgtgtcggtg tggcgcgaca atttcctgcg cgatctgcag cacgtctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t