SSGCID: Target Page

BuceA.00046.a

Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

CENTER ID:	BuceA.00046.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00046.a.B1.GE36638	Full length( BuceA.00046.a )		1	474

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00046.a.B1.PS01808	Full length( BuceA.00046.a )		1	474

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2587
RefSeq:	YP_002231596.1
UniProt:	B4E738

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRLIVVSNR VAAGEDTRPS AGGLAVGVMD ALRETGGVWF GWNGEIVGAP DAEPAIQRDG NVTYATVGLT RRDYDQYYRG FSNATLWPVF HYRGDLARFD RQEYAGYLRV NAMLAKQLAA LLRPDDLIWV HDYHLLPFAH CLRELGVKNP IGFFLHIPFP SPDMLRLVPP HEELVKFMCA YDVAGFQTEA DKQAFTDYIE RRGIGAASDD DGMLHAHGRV VKVAAYPIGV HPDAIAQAAV QYGTRKPVKM LREALGDRKL VMSVDRLDYS KGLVERFQSF ERMLANAPGW QGRVSLLQIA PPTRSDVQTY QHIRETLEGE AGRINGRFSQ LDWTPIQYLN RKYERNLLMA FFRMSQVGYV TPLRDGMNLV AKEYVASQDP ADPGVLVLSE FAGAAAELTG ALLVNPYDLS QMAEALERAL SMPLAERQAR HEENLARLRE NDLSVWRDTF VADLRSVAAA ASVTRRAGRR VAHA

NT Sequence

ATGAGCAGAT TGATCGTGGT ATCGAATCGT GTCGCCGCCG GCGAGGATAC GCGCCCGAGC GCGGGCGGCC TGGCCGTCGG CGTGATGGAC GCGCTGCGGG AAACGGGCGG CGTCTGGTTC GGCTGGAACG GCGAGATCGT CGGCGCGCCC GATGCCGAGC CCGCGATCCA GCGGGACGGC AACGTGACGT ATGCGACGGT CGGGTTGACC CGGCGCGACT ACGACCAGTA CTACCGCGGC TTCTCGAACG CGACGCTGTG GCCGGTGTTC CACTACCGCG GCGATCTCGC GCGCTTCGAT CGGCAGGAGT ACGCCGGTTA TCTGCGCGTG AACGCGATGC TCGCGAAGCA GCTCGCCGCG CTGCTGCGTC CGGACGACCT GATCTGGGTG CACGACTATC ACCTGCTGCC GTTCGCGCAC TGCCTGCGCG AGCTCGGCGT GAAGAACCCG ATCGGCTTCT TCCTGCACAT CCCGTTTCCG TCGCCCGACA TGCTGCGCCT CGTGCCGCCG CACGAGGAAC TCGTGAAGTT CATGTGCGCG TACGACGTCG CGGGCTTCCA GACCGAAGCC GACAAGCAGG CCTTCACCGA CTACATCGAG CGGCGCGGCA TCGGCGCGGC GAGCGACGAC GACGGGATGC TGCATGCGCA TGGCCGTGTC GTGAAGGTCG CCGCCTATCC GATCGGCGTG CATCCCGACG CGATCGCGCA GGCGGCCGTT CAGTACGGCA CGCGCAAGCC GGTGAAGATG CTGCGCGAGG CGCTGGGCGA CCGCAAGCTG GTGATGAGCG TCGATCGCCT CGATTACTCG AAGGGGCTCG TCGAACGCTT CCAGTCGTTC GAGCGGATGC TCGCGAATGC GCCGGGCTGG CAGGGACGCG TGTCGCTCCT GCAGATCGCG CCGCCGACAC GTTCCGACGT GCAGACCTAT CAGCACATCC GCGAAACGCT CGAAGGCGAG GCCGGCCGCA TCAACGGCCG CTTCTCGCAA CTCGATTGGA CGCCGATCCA GTACCTGAAC CGCAAGTACG AGCGCAACCT GCTGATGGCG TTCTTCCGGA TGTCGCAGGT CGGCTACGTG ACGCCGCTGC GCGACGGGAT GAATCTCGTC GCGAAGGAAT ACGTCGCGTC GCAGGATCCG GCCGATCCGG GCGTGCTCGT GCTGTCGGAG TTCGCGGGCG CGGCGGCCGA GCTGACCGGC GCGCTGCTCG TCAATCCGTA CGACCTGTCG CAGATGGCCG AGGCGCTCGA ACGCGCGCTG TCGATGCCGC TCGCGGAGCG GCAGGCGCGT CACGAGGAGA ACCTTGCGCG GCTGCGCGAG AACGACCTGT CGGTCTGGCG CGACACGTTC GTCGCCGATT TGCGCAGTGT CGCGGCGGCG GCGTCGGTCA CGCGGCGCGC GGGCCGGCGG GTGGCGCATG CG

Details for BuceA.00046.a.B1.GE36638

HARVESTED ON:	2/8/2013
SEQUENCED ON:	2/14/2013
EXPECTED MW:	54kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	90

Validated AA Sequence

MDALRETGGV WFGWNGEIVG APDAEPAIQR DGNVTYATVG LTRRDYDQYY RGFSNATLWP VFHYRGDLAR FDRQEYAGYL RVNAMLAKQL AALLRPDDLI WVHDYHLLPF AHCLRELGVK NPIGFFLHIP FPSPDMLRLV PPHEELVKFM CAYDVAGFQT EADKQAFTDY IERRGIGAAS DDDGMLHAHG RVVKVAAYPI GVHPDAIAQA AVQYGTRKPV KMLREALGDR KLVMSVDRLD YSKGLVERFQ SFERMLANAP GWQGRVSLLQ IAPPTRSDVQ TYQHIRETLE GEAGRINGRF SQLDWTPIQY LNRKYERNLL MAFFRMSQVG YVTPLRDGMN LVAKEYVASQ DPADPGVLVL SEFAGAAAEL TGALLVNPYD LSQMAEALER ALSMPLAERQ ARHEENLARL RENDLSVWRD TFVADLRSVA AAASVTRR

Validated NT Sequence

cgcgccgcgt gaccgacgcc gccgccgcga cactgcgcaa atcggcgacg aacgtgtcgc gccagaccga caggtcgttc tcgcgcagcc gcgcaaggtt ctcctcgtga cgcgcctgcc gctccgcgag cggcatcgac agcgcgcgtt cgagcgcctc ggccatctgc gacaggtcgt acggattgac gagcagcgcg ccggtcagct cggccgccgc gcccgcgaac tccgacagca cgagcacgcc cggatcggcc ggatcctgcg acgcgacgta ttccttcgcg acgagattca tcccgtcgcg cagcggcgtc acgtagccga cctgcgacat ccggaagaac gccatcagca ggttgcgctc gtacttgcgg ttcaggtact ggatcggcgt ccaatcgagt tgcgagaagc ggccgttgat gcggccggcc tcgccttcga gcgtttcgcg gatgtgctga taggtctgca cgtcggaacg tgtcggcggc gcgatctgca ggagcgacac gcgtccctgc cagcccggcg cattcgcgag catccgctcg aacgactgga agcgttcgac gagccccttc gagtaatcga ggcgatcgac gctcatcacc agcttgcggt cgcccagcgc ctcgcgcagc atcttcaccg gcttgcgcgt gccgtactga acggccgcct gcgcgatcgc gtcgggatgc acgccgatcg gataggcggc gaccttcacg acacggccat gcgcatgcag catcccgtcg tcgtcgctcg ccgcgccgat gccgcgccgc tcgatgtagt cggtgaaggc ctgcttgtcg gcttcggtct ggaagcccgc gacgtcgtac gcgcacatga acttcacgag ttcctcgtgc ggcggcacga ggcgcagcat gtcgggcgac ggaaacggga tgtgcaggaa gaagccgatc gggttcttca cgccgagctc gcgcaggcag tgcgcgaacg gcagcaggtg atagtcgtgc acccagatca ggtcgtccgg acgcagcagc gcggcgagct gcttcgcgag catcgcgttc acgcgcagat aaccggcgta ctcctgccga tcgaagcgcg cgagatcgcc gcggtagtgg aacaccggcc acagcgtcgc gttcgagaag ccgcggtagt actggtcgta gtcgcgccgg gtcaacccga ccgtcgcata cgtcacgttg ccgtcccgct ggatcgcggg ctcggcatcg ggcgcgccga cgatctcgcc gttccagccg aaccagacgc cgcccgtttc ccgcagcgcg tccatcacgc cgacggccag gccgcccgcg ctcgggcgcg tatcctcgcc ggcggcgaca cgattcgata ccacgatcaa t

Expected Protein Sequence

MAHHHHHHMS RLIVVSNRVA AGEDTRPSAG GLAVGVMDAL RETGGVWFGW NGEIVGAPDA EPAIQRDGNV TYATVGLTRR DYDQYYRGFS NATLWPVFHY RGDLARFDRQ EYAGYLRVNA MLAKQLAALL RPDDLIWVHD YHLLPFAHCL RELGVKNPIG FFLHIPFPSP DMLRLVPPHE ELVKFMCAYD VAGFQTEADK QAFTDYIERR GIGAASDDDG MLHAHGRVVK VAAYPIGVHP DAIAQAAVQY GTRKPVKMLR EALGDRKLVM SVDRLDYSKG LVERFQSFER MLANAPGWQG RVSLLQIAPP TRSDVQTYQH IRETLEGEAG RINGRFSQLD WTPIQYLNRK YERNLLMAFF RMSQVGYVTP LRDGMNLVAK EYVASQDPAD PGVLVLSEFA GAAAELTGAL LVNPYDLSQM AEALERALSM PLAERQARHE ENLARLREND LSVWRDTFVA DLRSVAAAAS VTRRAGRRVA HA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca gattgatcgt ggtatcgaat cgtgtcgccg ccggcgagga tacgcgcccg agcgcgggcg gcctggccgt cggcgtgatg gacgcgctgc gggaaacggg cggcgtctgg ttcggctgga acggcgagat cgtcggcgcg cccgatgccg agcccgcgat ccagcgggac ggcaacgtga cgtatgcgac ggtcgggttg acccggcgcg actacgacca gtactaccgc ggcttctcga acgcgacgct gtggccggtg ttccactacc gcggcgatct cgcgcgcttc gatcggcagg agtacgccgg ttatctgcgc gtgaacgcga tgctcgcgaa gcagctcgcc gcgctgctgc gtccggacga cctgatctgg gtgcacgact atcacctgct gccgttcgcg cactgcctgc gcgagctcgg cgtgaagaac ccgatcggct tcttcctgca catcccgttt ccgtcgcccg acatgctgcg cctcgtgccg ccgcacgagg aactcgtgaa gttcatgtgc gcgtacgacg tcgcgggctt ccagaccgaa gccgacaagc aggccttcac cgactacatc gagcggcgcg gcatcggcgc ggcgagcgac gacgacggga tgctgcatgc gcatggccgt gtcgtgaagg tcgccgccta tccgatcggc gtgcatcccg acgcgatcgc gcaggcggcc gttcagtacg gcacgcgcaa gccggtgaag atgctgcgcg aggcgctggg cgaccgcaag ctggtgatga gcgtcgatcg cctcgattac tcgaaggggc tcgtcgaacg cttccagtcg ttcgagcgga tgctcgcgaa tgcgccgggc tggcagggac gcgtgtcgct cctgcagatc gcgccgccga cacgttccga cgtgcagacc tatcagcaca tccgcgaaac gctcgaaggc gaggccggcc gcatcaacgg ccgcttctcg caactcgatt ggacgccgat ccagtacctg aaccgcaagt acgagcgcaa cctgctgatg gcgttcttcc ggatgtcgca ggtcggctac gtgacgccgc tgcgcgacgg gatgaatctc gtcgcgaagg aatacgtcgc gtcgcaggat ccggccgatc cgggcgtgct cgtgctgtcg gagttcgcgg gcgcggcggc cgagctgacc ggcgcgctgc tcgtcaatcc gtacgacctg tcgcagatgg ccgaggcgct cgaacgcgcg ctgtcgatgc cgctcgcgga gcggcaggcg cgtcacgagg agaaccttgc gcggctgcgc gagaacgacc tgtcggtctg gcgcgacacg ttcgtcgccg atttgcgcag tgtcgcggcg gcggcgtcgg tcacgcggcg cgcgggccgg cgggtggcgc atgcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for BuceA.00046.a.B1.PS01808

PURIFICATION DATe:	4/24/2013
CONCENTRATION:	14.6mg/ml
OBSERVED MW:	51kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	90

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA GATTGATCGT GGTATCGAAT CGTGTCGCCG CCGGCGAGGA TACGCGCCCG AGCGCGGGCG GCCTGGCCGT CGGCGTGATG GACGCGCTGC GGGAAACGGG CGGCGTCTGG TTCGGCTGGA ACGGCGAGAT CGTCGGCGCG CCCGATGCCG AGCCCGCGAT CCAGCGGGAC GGCAACGTGA CGTATGCGAC GGTCGGGTTG ACCCGGCGCG ACTACGACCA GTACTACCGC GGCTTCTCGA ACGCGACGCT GTGGCCGGTG TTCCACTACC GCGGCGATCT CGCGCGCTTC GATCGGCAGG AGTACGCCGG TTATCTGCGC GTGAACGCGA TGCTCGCGAA GCAGCTCGCC GCGCTGCTGC GTCCGGACGA CCTGATCTGG GTGCACGACT ATCACCTGCT GCCGTTCGCG CACTGCCTGC GCGAGCTCGG CGTGAAGAAC CCGATCGGCT TCTTCCTGCA CATCCCGTTT CCGTCGCCCG ACATGCTGCG CCTCGTGCCG CCGCACGAGG AACTCGTGAA GTTCATGTGC GCGTACGACG TCGCGGGCTT CCAGACCGAA GCCGACAAGC AGGCCTTCAC CGACTACATC GAGCGGCGCG GCATCGGCGC GGCGAGCGAC GACGACGGGA TGCTGCATGC GCATGGCCGT GTCGTGAAGG TCGCCGCCTA TCCGATCGGC GTGCATCCCG ACGCGATCGC GCAGGCGGCC GTTCAGTACG GCACGCGCAA GCCGGTGAAG ATGCTGCGCG AGGCGCTGGG CGACCGCAAG CTGGTGATGA GCGTCGATCG CCTCGATTAC TCGAAGGGGC TCGTCGAACG CTTCCAGTCG TTCGAGCGGA TGCTCGCGAA TGCGCCGGGC TGGCAGGGAC GCGTGTCGCT CCTGCAGATC GCGCCGCCGA CACGTTCCGA CGTGCAGACC TATCAGCACA TCCGCGAAAC GCTCGAAGGC GAGGCCGGCC GCATCAACGG CCGCTTCTCG CAACTCGATT GGACGCCGAT CCAGTACCTG AACCGCAAGT ACGAGCGCAA CCTGCTGATG GCGTTCTTCC GGATGTCGCA GGTCGGCTAC GTGACGCCGC TGCGCGACGG GATGAATCTC GTCGCGAAGG AATACGTCGC GTCGCAGGAT CCGGCCGATC CGGGCGTGCT CGTGCTGTCG GAGTTCGCGG GCGCGGCGGC CGAGCTGACC GGCGCGCTGC TCGTCAATCC GTACGACCTG TCGCAGATGG CCGAGGCGCT CGAACGCGCG CTGTCGATGC CGCTCGCGGA GCGGCAGGCG CGTCACGAGG AGAACCTTGC GCGGCTGCGC GAGAACGACC TGTCGGTCTG GCGCGACACG TTCGTCGCCG ATTTGCGCAG TGTCGCGGCG GCGGCGTCGG TCACGCGGCG CGCGGGCCGG CGGGTGGCGC ATGCGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT