SSGCID: Target Page

BuceA.00045.a

DNA polymerase III, epsilon chain (EC 2.7.7.7)

CENTER ID:	BuceA.00045.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00045.a.B1.GE35006	full length		1	244

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.1299
RefSeq:	YP_002230392.1
UniProt:	B4EEJ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRQIILDTET TGLNARTGDR LIEIGCVELL NRRLTGNNLH FYVNPERDSD PGALAVHGLT TEFLSDKPKF AEVAHQILDF VKDAELIIHN APFDLGFLDA EFARLGLPPF TEHCGGVIDT LVQAKQMFPG KRNSLDALCD RFGISNAHRT LHGALLDSEL LAEVYLAMTR GQDSLVIDML DDASADGGAA NGKRVSLAAL DLPVVVASDD ELAAHQAQLD ELDKSVKGTC VWRQPADTDT AEAA

NT Sequence

atgcgccaga tcattctcga taccgaaacc accggcctga acgcccgcac gggcgaccgc ctcatcgaaa tcggctgcgt cgagctgctg aaccggcggc tcaccggcaa caacctgcac ttctacgtga accccgagcg cgacagcgat ccgggcgcac tggcggtgca cggcctcacg accgaattct tgagcgacaa gccgaaattc gcggaagtcg cccaccagat cctcgacttc gtgaaggacg ccgagctgat catccacaac gcgccgttcg acctgggctt cctcgacgcc gaattcgcgc ggctcggcct gccgccgttc accgagcatt gcggcggcgt gatcgacacg ctggtgcagg ccaagcagat gttccccggc aagcgcaact cgctcgacgc gctgtgcgac cgcttcggca tcagcaacgc gcaccgcacg ctgcacggcg cgctgctcga ctcggagctg ctcgccgagg tgtatctcgc gatgacgcgc ggccaggaca gcctcgtgat cgacatgctc gacgacgcga gcgccgacgg cggcgccgcg aacggcaagc gcgtgtcgct cgccgcgctc gacctgcctg tagtggtcgc aagcgacgac gagttggccg cgcaccaggc tcagctcgac gagctcgaca agtcggtcaa gggcacctgc gtgtggcgcc agcccgccga cacggatacc gccgaagcgg cctga

Details for BuceA.00045.a.B1.GE35006

HARVESTED ON:	3/19/2012
SEQUENCED ON:	3/22/2012
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

Validated NT Sequence

ttactcactt aggccgcttc ggcggtatcc gtgtcggcgg gctggcgcca cacgcaggtg cccttgaccg acttgtcgag ctcgtcgagc tgagcctggt gcgcggccaa ctcgtcgtcg cttgcgacca ctacaggcag gtcgagcgcg gcgagcgaca cgcgcttgcc gttcgcggcg ccgccgtcgg cgctcgcgtc gtcgagcatg tcgatcacga ggctgtcctg gccgcgcgtc atcgcgagat acacctcggc gagcagctcc gagtcgagca gcgcgccgtg cagcgtgcgg tgcgcgttgc tgatgccgaa gcggtcgcac agcgcgtcga gcgagttgcg cttgccgggg aacatctgct tggcctgcac cagcgtgtcg atcacgccgc cgcaatgctc ggtgaacggc ggcaggccga gccgcgcgaa ttcggcgtcg aggaagccca ggtcgaacgg cgcgttgtgg atgatcagct cggcgtcctt cacgaagtcg aggatctggt gggcgacttc cgcgaatttc ggcttgtcgc tcaagaattc ggtcgtgagg ccgtgcaccg ccagtgcgcc cggatcgctg tcgcgctcgg ggttcacgta gaagtgcagg ttgttgccgg tgagccgccg gttcagcagc tcgacgcagc cgatttcgat gaggcggtcg cccgtgcggg cgttcaggcc ggtggtttcg gtatcgagaa tgatctggcg catatggtgg tggtgg

Expected Protein Sequence

MAHHHHHHMR QIILDTETTG LNARTGDRLI EIGCVELLNR RLTGNNLHFY VNPERDSDPG ALAVHGLTTE FLSDKPKFAE VAHQILDFVK DAELIIHNAP FDLGFLDAEF ARLGLPPFTE HCGGVIDTLV QAKQMFPGKR NSLDALCDRF GISNAHRTLH GALLDSELLA EVYLAMTRGQ DSLVIDMLDD ASADGGAANG KRVSLAALDL PVVVASDDEL AAHQAQLDEL DKSVKGTCVW RQPADTDTAE AA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcc agatcattct cgataccgaa accaccggcc tgaacgcccg cacgggcgac cgcctcatcg aaatcggctg cgtcgagctg ctgaaccggc ggctcaccgg caacaacctg cacttctacg tgaaccccga gcgcgacagc gatccgggcg cactggcggt gcacggcctc acgaccgaat tcttgagcga caagccgaaa ttcgcggaag tcgcccacca gatcctcgac ttcgtgaagg acgccgagct gatcatccac aacgcgccgt tcgacctggg cttcctcgac gccgaattcg cgcggctcgg cctgccgccg ttcaccgagc attgcggcgg cgtgatcgac acgctggtgc aggccaagca gatgttcccc ggcaagcgca actcgctcga cgcgctgtgc gaccgcttcg gcatcagcaa cgcgcaccgc acgctgcacg gcgcgctgct cgactcggag ctgctcgccg aggtgtatct cgcgatgacg cgcggccagg acagcctcgt gatcgacatg ctcgacgacg cgagcgccga cggcggcgcc gcgaacggca agcgcgtgtc gctcgccgcg ctcgacctgc ctgtagtggt cgcaagcgac gacgagttgg ccgcgcacca ggctcagctc gacgagctcg acaagtcggt caagggcacc tgcgtgtggc gccagcccgc cgacacggat accgccgaag cggcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat