BuceA.00043.a
Dihydroorotase
| CENTER ID: | BuceA.00043.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00043.a.B1.GE36637 | Full length( BuceA.00043.a ) | 1 | 364 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00043.a.B1.PS01797 | Full length( BuceA.00043.a ) | 1 | 364 |
Structures
4LFY
DEPOSITED: 6/27/2013
DETERMINATION: XRay
CLONE: BuceA.00043.a.B1.GE36637
PROTEIN: BuceA.00043.a.B1.PS01797
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130129 |
| PATRIC ID: | fig|216591.5.peg.3476 |
| RefSeq: | YP_002232455.1 |
| UniProt: | B4EEU0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARPFLTTPP MTASNASSPA TLSLARPDDW HLHLRDGDML AAVLPHTARQ FGRAIVMPNL KPPVTTTAQA QAYRERILAA LPAGMTFEPL MTLYLTDNTP PDEIRRARES GFVHGVKLYP AGATTNSDHG VTDLAKCAKT LEAMQETGMP LLVHGEVTDA SIDLFDREKV FIDRVMTPLR RDFPGLKVVF EHITTKDAAD YVRDADAAPG LLGATITAHH LLYNRNALFV GGIRPHYYCL PVLKRETHRV ALVEAATSGN PRFFLGTDSA PHARDAKETA CGCAGCYTAL HALELYAEAF DTAGALDKLE GFASFFGADF YGLPRSAETV TLRREPWELP REIFAGETPV VPLRGGETIG WKLA
NT Sequence
ATGGCACGCC CGTTCCTCAC GACGCCTCCT ATGACTGCCT CGAACGCTTC TTCCCCGGCG ACGCTTTCGC TCGCCCGTCC CGACGACTGG CACCTGCACC TGCGAGACGG CGACATGCTC GCCGCCGTGC TGCCGCACAC CGCCCGCCAG TTCGGCCGCG CCATCGTCAT GCCGAACCTG AAGCCGCCGG TCACGACCAC CGCGCAGGCG CAGGCGTATC GCGAGCGCAT CCTCGCCGCG CTGCCGGCCG GGATGACGTT CGAGCCGCTG ATGACGCTGT ACCTGACCGA CAACACGCCG CCCGACGAAA TCCGCCGCGC GCGCGAAAGC GGCTTCGTGC ACGGCGTGAA GCTGTATCCG GCAGGCGCCA CGACGAATTC CGACCATGGC GTCACCGATC TCGCGAAATG TGCGAAGACG CTCGAGGCGA TGCAGGAAAC CGGCATGCCG CTGCTCGTGC ACGGCGAGGT GACCGATGCG TCGATCGACC TGTTCGACCG CGAGAAGGTG TTCATCGACC GCGTGATGAC GCCGCTGCGC CGCGATTTCC CGGGCCTGAA GGTCGTGTTC GAACACATCA CGACGAAGGA CGCGGCCGAC TACGTCCGCG ATGCCGACGC GGCGCCCGGC CTGCTCGGCG CGACGATCAC CGCGCATCAC CTGCTGTACA ACCGCAACGC GCTGTTCGTC GGCGGGATCC GCCCGCATTA CTACTGCCTG CCGGTGCTGA AGCGCGAGAC GCATCGCGTG GCGCTGGTCG AGGCCGCGAC GTCGGGCAAC CCGCGCTTCT TCCTCGGCAC CGACAGCGCG CCGCACGCGC GCGACGCGAA GGAAACCGCG TGCGGTTGCG CGGGCTGCTA CACGGCGCTG CACGCGCTCG AGCTGTACGC GGAAGCGTTC GACACGGCCG GCGCGCTCGA CAAGCTGGAA GGGTTCGCGA GCTTCTTCGG CGCCGATTTC TACGGGCTGC CGCGCAGCGC CGAGACGGTC ACGCTGCGCC GCGAGCCGTG GGAACTGCCG CGCGAGATCT TCGCGGGCGA GACGCCGGTC GTGCCGCTGC GCGGCGGCGA GACGATCGGC TGGAAACTGG CG
Details for BuceA.00043.a.B1.GE36637
| HARVESTED ON: | 2/8/2013 |
| SEQUENCED ON: | 2/14/2013 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MTASNASSPA TLSLARPDDW HLHLRDGDML AAVLPHTARQ FGRAIVMPNL KPPVTTTAQA QAYRERILAA LPAGMTFEPL MTLYLTDNTP PDEIRRARES GFVHGVKLYP AGATTNSDHG VTDLAKCAKT LEAMQETGMP LLVHGEVTDA SIDLFDREKV FIDRVMTPLR RDFPGLKVVF EHITTKDAAD YVRDADAAPG LLGATITAHH LLYNRNALFV GGIRPHYYCL PVLKRETHRV ALVEAATSGN PRFFLGTDSA PHARDAKETA CGCAGCYTAL HALELYAEAF DTAGALDKLE GFASFFGADF YGLPRSAETV TLRREPWELP REIFAGETPV VPLRGGETIG WKLA
Validated NT Sequence
tactcactta cgccagtttc cagccgatcg tctcgccgcc gcgcagcggc acgaccggcg tctcgcccgc gaagatctcg cgcggcagtt cccacggctc gcggcgcagc gtgaccgtct cggcgctgcg cggcagcccg tagaaatcgg cgccgaagaa gctcgcgaac ccttccagct tgtcgagcgc gccggccgtg tcgaacgctt ccgcgtacag ctcgagcgcg tgcagcgccg tgtagcagcc cgcgcaaccg cacgcggttt ccttcgcgtc gcgcgcgtgc ggcgcgctgt cggtgccgag gaagaagcgc gggttgcccg acgtcgcggc ctcgaccagc gccacgcgat gcgtctcgcg cttcagcacc ggcaggcagt agtaatgcgg gcggatcccg ccgacgaaca gcgcgttgcg gttgtacagc aggtgatgcg cggtgatcgt cgcgccgagc aggccgggcg ccgcgtcggc atcgcggacg tagtcggccg cgtccttcgt cgtgatgtgt tcgaacacga ccttcaggcc cgggaaatcg cggcgcagcg gcgtcatcac gcggtcgatg aacaccttct cgcggtcgaa caggtcgatc gacgcatcgg tcacctcgcc gtgcacgagc agcggcatgc cggtttcctg catcgcctcg agcgtcttcg cacatttcgc gagatcggtg acgccatggt cggaattcgt cgtggcgcct gccggataca gcttcacgcc gtgcacgaag ccgctttcgc gcgcgcggcg gatttcgtcg ggcggcgtgt tgtcggtcag gtacagcgtc atcagcggct cgaacgtcat cccggccggc agcgcggcga ggatgcgctc gcgatacgcc tgcgcctgcg cggtggtcgt gaccggcggc ttcaggttcg gcatgacgat ggcgcggccg aactggcggg cggtgtgcgg cagcacggcg gcgagcatgt cgccgtctcg caggtgcagg tgccagtcgt cgggacgggc gagcgaaagc gtcgccgggg aagaagcgtt cgaggcagtc ataggaggcg tcgtgaggaa cgggcg
Expected Protein Sequence
MAHHHHHHMA RPFLTTPPMT ASNASSPATL SLARPDDWHL HLRDGDMLAA VLPHTARQFG RAIVMPNLKP PVTTTAQAQA YRERILAALP AGMTFEPLMT LYLTDNTPPD EIRRARESGF VHGVKLYPAG ATTNSDHGVT DLAKCAKTLE AMQETGMPLL VHGEVTDASI DLFDREKVFI DRVMTPLRRD FPGLKVVFEH ITTKDAADYV RDADAAPGLL GATITAHHLL YNRNALFVGG IRPHYYCLPV LKRETHRVAL VEAATSGNPR FFLGTDSAPH ARDAKETACG CAGCYTALHA LELYAEAFDT AGALDKLEGF ASFFGADFYG LPRSAETVTL RREPWELPRE IFAGETPVVP LRGGETIGWK LA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac gcccgttcct cacgacgcct cctatgactg cctcgaacgc ttcttccccg gcgacgcttt cgctcgcccg tcccgacgac tggcacctgc acctgcgaga cggcgacatg ctcgccgccg tgctgccgca caccgcccgc cagttcggcc gcgccatcgt catgccgaac ctgaagccgc cggtcacgac caccgcgcag gcgcaggcgt atcgcgagcg catcctcgcc gcgctgccgg ccgggatgac gttcgagccg ctgatgacgc tgtacctgac cgacaacacg ccgcccgacg aaatccgccg cgcgcgcgaa agcggcttcg tgcacggcgt gaagctgtat ccggcaggcg ccacgacgaa ttccgaccat ggcgtcaccg atctcgcgaa atgtgcgaag acgctcgagg cgatgcagga aaccggcatg ccgctgctcg tgcacggcga ggtgaccgat gcgtcgatcg acctgttcga ccgcgagaag gtgttcatcg accgcgtgat gacgccgctg cgccgcgatt tcccgggcct gaaggtcgtg ttcgaacaca tcacgacgaa ggacgcggcc gactacgtcc gcgatgccga cgcggcgccc ggcctgctcg gcgcgacgat caccgcgcat cacctgctgt acaaccgcaa cgcgctgttc gtcggcggga tccgcccgca ttactactgc ctgccggtgc tgaagcgcga gacgcatcgc gtggcgctgg tcgaggccgc gacgtcgggc aacccgcgct tcttcctcgg caccgacagc gcgccgcacg cgcgcgacgc gaaggaaacc gcgtgcggtt gcgcgggctg ctacacggcg ctgcacgcgc tcgagctgta cgcggaagcg ttcgacacgg ccggcgcgct cgacaagctg gaagggttcg cgagcttctt cggcgccgat ttctacgggc tgccgcgcag cgccgagacg gtcacgctgc gccgcgagcc gtgggaactg ccgcgcgaga tcttcgcggg cgagacgccg gtcgtgccgc tgcgcggcgg cgagacgatc ggctggaaac tggcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BuceA.00043.a.B1.PS01797
| PURIFICATION DATe: | 4/10/2013 |
| CONCENTRATION: | 75mg/ml |
| OBSERVED MW: | 39kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTASNASSPA TLSLARPDDW HLHLRDGDML AAVLPHTARQ FGRAIVMPNL KPPVTTTAQA QAYRERILAA LPAGMTFEPL MTLYLTDNTP PDEIRRARES GFVHGVKLYP AGATTNSDHG VTDLAKCAKT LEAMQETGMP LLVHGEVTDA SIDLFDREKV FIDRVMTPLR RDFPGLKVVF EHITTKDAAD YVRDADAAPG LLGATITAHH LLYNRNALFV GGIRPHYYCL PVLKRETHRV ALVEAATSGN PRFFLGTDSA PHARDAKETA CGCAGCYTAL HALELYAEAF DTAGALDKLE GFASFFGADF YGLPRSAETV TLRREPWELP REIFAGETPV VPLRGGETIG WKLA
Validated NT Sequence
tactcactta cgccagtttc cagccgatcg tctcgccgcc gcgcagcggc acgaccggcg tctcgcccgc gaagatctcg cgcggcagtt cccacggctc gcggcgcagc gtgaccgtct cggcgctgcg cggcagcccg tagaaatcgg cgccgaagaa gctcgcgaac ccttccagct tgtcgagcgc gccggccgtg tcgaacgctt ccgcgtacag ctcgagcgcg tgcagcgccg tgtagcagcc cgcgcaaccg cacgcggttt ccttcgcgtc gcgcgcgtgc ggcgcgctgt cggtgccgag gaagaagcgc gggttgcccg acgtcgcggc ctcgaccagc gccacgcgat gcgtctcgcg cttcagcacc ggcaggcagt agtaatgcgg gcggatcccg ccgacgaaca gcgcgttgcg gttgtacagc aggtgatgcg cggtgatcgt cgcgccgagc aggccgggcg ccgcgtcggc atcgcggacg tagtcggccg cgtccttcgt cgtgatgtgt tcgaacacga ccttcaggcc cgggaaatcg cggcgcagcg gcgtcatcac gcggtcgatg aacaccttct cgcggtcgaa caggtcgatc gacgcatcgg tcacctcgcc gtgcacgagc agcggcatgc cggtttcctg catcgcctcg agcgtcttcg cacatttcgc gagatcggtg acgccatggt cggaattcgt cgtggcgcct gccggataca gcttcacgcc gtgcacgaag ccgctttcgc gcgcgcggcg gatttcgtcg ggcggcgtgt tgtcggtcag gtacagcgtc atcagcggct cgaacgtcat cccggccggc agcgcggcga ggatgcgctc gcgatacgcc tgcgcctgcg cggtggtcgt gaccggcggc ttcaggttcg gcatgacgat ggcgcggccg aactggcggg cggtgtgcgg cagcacggcg gcgagcatgt cgccgtctcg caggtgcagg tgccagtcgt cgggacgggc gagcgaaagc gtcgccgggg aagaagcgtt cgaggcagtc ataggaggcg tcgtgaggaa cgggcg
Expressed Protein Sequence
MAHHHHHHMA RPFLTTPPMT ASNASSPATL SLARPDDWHL HLRDGDMLAA VLPHTARQFG RAIVMPNLKP PVTTTAQAQA YRERILAALP AGMTFEPLMT LYLTDNTPPD EIRRARESGF VHGVKLYPAG ATTNSDHGVT DLAKCAKTLE AMQETGMPLL VHGEVTDASI DLFDREKVFI DRVMTPLRRD FPGLKVVFEH ITTKDAADYV RDADAAPGLL GATITAHHLL YNRNALFVGG IRPHYYCLPV LKRETHRVAL VEAATSGNPR FFLGTDSAPH ARDAKETACG CAGCYTALHA LELYAEAFDT AGALDKLEGF ASFFGADFYG LPRSAETVTL RREPWELPRE IFAGETPVVP LRGGETIGWK LA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAC GCCCGTTCCT CACGACGCCT CCTATGACTG CCTCGAACGC TTCTTCCCCG GCGACGCTTT CGCTCGCCCG TCCCGACGAC TGGCACCTGC ACCTGCGAGA CGGCGACATG CTCGCCGCCG TGCTGCCGCA CACCGCCCGC CAGTTCGGCC GCGCCATCGT CATGCCGAAC CTGAAGCCGC CGGTCACGAC CACCGCGCAG GCGCAGGCGT ATCGCGAGCG CATCCTCGCC GCGCTGCCGG CCGGGATGAC GTTCGAGCCG CTGATGACGC TGTACCTGAC CGACAACACG CCGCCCGACG AAATCCGCCG CGCGCGCGAA AGCGGCTTCG TGCACGGCGT GAAGCTGTAT CCGGCAGGCG CCACGACGAA TTCCGACCAT GGCGTCACCG ATCTCGCGAA ATGTGCGAAG ACGCTCGAGG CGATGCAGGA AACCGGCATG CCGCTGCTCG TGCACGGCGA GGTGACCGAT GCGTCGATCG ACCTGTTCGA CCGCGAGAAG GTGTTCATCG ACCGCGTGAT GACGCCGCTG CGCCGCGATT TCCCGGGCCT GAAGGTCGTG TTCGAACACA TCACGACGAA GGACGCGGCC GACTACGTCC GCGATGCCGA CGCGGCGCCC GGCCTGCTCG GCGCGACGAT CACCGCGCAT CACCTGCTGT ACAACCGCAA CGCGCTGTTC GTCGGCGGGA TCCGCCCGCA TTACTACTGC CTGCCGGTGC TGAAGCGCGA GACGCATCGC GTGGCGCTGG TCGAGGCCGC GACGTCGGGC AACCCGCGCT TCTTCCTCGG CACCGACAGC GCGCCGCACG CGCGCGACGC GAAGGAAACC GCGTGCGGTT GCGCGGGCTG CTACACGGCG CTGCACGCGC TCGAGCTGTA CGCGGAAGCG TTCGACACGG CCGGCGCGCT CGACAAGCTG GAAGGGTTCG CGAGCTTCTT CGGCGCCGAT TTCTACGGGC TGCCGCGCAG CGCCGAGACG GTCACGCTGC GCCGCGAGCC GTGGGAACTG CCGCGCGAGA TCTTCGCGGG CGAGACGCCG GTCGTGCCGC TGCGCGGCGG CGAGACGATC GGCTGGAAAC TGGCGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT