SSGCID: Target Page

BuceA.00039.b

Methionine aminopeptidase

CENTER ID:	BuceA.00039.b
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00039.b.B1.GE36636	Full length( BuceA.00039.b )		1	268

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2949
RefSeq:	YP_002230175.1
UniProt:	B4ECI8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKREIPIRG AAEIAKSREA AKLASQVLTM ITEYVKPGVT TDELDARCRE YIIDELGAIP ANIGYHGYPK TLCTSVNHVV CHGIPTSRPM RDGDIVNLDI AVIKDGWFGD TSRMYFVGEP NELARRLVAA TYEAMHAGIR AVRPGATLGD VGYAIQQVAH REGFSVVREY CGHGIGDVYH DEPQVLHYGR PGTGLPLRPG MIFTIEPMLN AGKRDTHVLA DGWTVVTKDH SLSAQWEHMV VVTETGFEVL TEDAKPQAFS ALPGTHAA

NT Sequence

ATGGCGAAAC GTGAAATTCC GATCCGCGGT GCCGCGGAAA TCGCCAAGTC GCGCGAAGCC GCGAAGCTCG CGTCGCAGGT GCTGACGATG ATCACCGAGT ACGTGAAGCC GGGCGTCACC ACCGATGAGC TGGACGCACG CTGCCGCGAA TACATCATCG ACGAATTGGG CGCGATTCCC GCGAACATCG GCTACCACGG CTATCCGAAG ACGCTGTGCA CGTCGGTCAA CCACGTGGTC TGTCACGGCA TTCCGACGTC GCGGCCGATG CGCGACGGCG ACATCGTGAA CCTCGACATC GCGGTGATCA AGGACGGCTG GTTCGGCGAT ACGAGCCGCA TGTACTTCGT CGGCGAACCG AACGAACTCG CGCGGCGGCT CGTCGCGGCG ACCTACGAGG CAATGCACGC CGGCATTCGC GCGGTGCGTC CCGGCGCGAC GCTCGGCGAC GTCGGTTATG CGATCCAGCA GGTCGCCCAT CGTGAAGGGT TCAGCGTGGT GCGCGAGTAT TGCGGGCACG GCATCGGCGA CGTCTATCAC GACGAGCCGC AGGTGCTGCA TTACGGTCGC CCGGGCACCG GCCTGCCGCT GCGGCCGGGG ATGATCTTCA CGATCGAGCC GATGCTCAAC GCGGGCAAGC GCGACACCCA CGTGCTGGCC GACGGCTGGA CGGTCGTCAC GAAGGACCAT TCGCTGTCCG CGCAATGGGA GCACATGGTC GTCGTGACGG AAACGGGTTT CGAGGTGCTC ACCGAGGACG CGAAGCCCCA GGCGTTCTCG GCGCTTCCGG GCACGCACGC TGCC

Details for BuceA.00039.b.B1.GE36636

HARVESTED ON:	data unavailable
SEQUENCED ON:	2/14/2013
EXPECTED MW:	30kDa
OBSERVED MW:	data unavailable
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA KREIPIRGAA EIAKSREAAK LASQVLTMIT EYVKPGVTTD ELDARCREYI IDELGAIPAN IGYHGYPKTL CTSVNHVVCH GIPTSRPMRD GDIVNLDIAV IKDGWFGDTS RMYFVGEPNE LARRLVAATY EAMHAGIRAV RPGATLGDVG YAIQQVAHRE GFSVVREYCG HGIGDVYHDE PQVLHYGRPG TGLPLRPGMI FTIEPMLNAG KRDTHVLADG WTVVTKDHSL SAQWEHMVVV TETGFEVLTE DAKPQAFSAL PGTHAA

Validated NT Sequence

ttgtttaact ttaagaagga gatataccat ggctcaccac caccaccacc atatggcgaa acgtgaaatt ccgatccgcg gtgccgcgga aatcgccaag tcgcgcgaag ccgcgaagct cgcgtcgcag gtgctgacga tgatcaccga gtacgtgaag ccgggcgtca ccaccgatga gctggacgca cgctgccgcg aatacatcat cgacgaattg ggcgcgattc ccgcgaacat cggctaccac ggctatccga agacgctgtg cacgtcggtc aaccacgtgg tctgtcacgg cattccgacg tcgcggccga tgcgcgacgg cgacatcgtg aacctcgaca tcgcggtgat caaggacggc tggttcggcg atacgagccg catgtacttc gtcggcgaac cgaacgaact cgcgcggcgg ctcgtcgcgg cgacctacga ggcaatgcac gccggcattc gcgcggtgcg tcccggcgcg acgctcggcg acgtcggtta tgcgatccag caggtcgccc atcgtgaagg gttcagcgtg gtgcgcgagt attgcgggca cggcatcggc gacgtctatc acgacgagcc gcaggtgctg cattacggtc gcccgggcac cggcctgccg ctgcggccgg ggatgatctt cacgatcgag ccgatgctca acgcgggcaa gcgcgacacc cacgtgctgg ccgacggctg gacggtcgtc acgaaggacc attcgctgtc cgcgcaatgg gagcacatgg tcgtcgtgac ggaaacgggt ttcgaggtgc tcaccgagga cgcgaagccc caggcgttct cggcgcttcc gggcacgcac gctgcctaag tgagtaanat aggatccnnn nnnnnnnncc ccnnnnnnan nnnnnnnnnn nnnnnnnnnn nannntgtgn nncnnnnngg gnnnnnngnn nntnnnnnnn nnnnncnnnn nnnnnnnnnn nnntgnnnnc ntnnnnnnnn nnnnnnnnnn nnncgnnnnn nnnnnnnnng gnngtnngnn ncnngggnnn ncnnnngtnn nnnnnnnnnn nngncccnnn nnnnnnnncn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cnnnnnnnnn nnnnnnnnnn nnnnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga aacgtgaaat tccgatccgc ggtgccgcgg aaatcgccaa gtcgcgcgaa gccgcgaagc tcgcgtcgca ggtgctgacg atgatcaccg agtacgtgaa gccgggcgtc accaccgatg agctggacgc acgctgccgc gaatacatca tcgacgaatt gggcgcgatt cccgcgaaca tcggctacca cggctatccg aagacgctgt gcacgtcggt caaccacgtg gtctgtcacg gcattccgac gtcgcggccg atgcgcgacg gcgacatcgt gaacctcgac atcgcggtga tcaaggacgg ctggttcggc gatacgagcc gcatgtactt cgtcggcgaa ccgaacgaac tcgcgcggcg gctcgtcgcg gcgacctacg aggcaatgca cgccggcatt cgcgcggtgc gtcccggcgc gacgctcggc gacgtcggtt atgcgatcca gcaggtcgcc catcgtgaag ggttcagcgt ggtgcgcgag tattgcgggc acggcatcgg cgacgtctat cacgacgagc cgcaggtgct gcattacggt cgcccgggca ccggcctgcc gctgcggccg gggatgatct tcacgatcga gccgatgctc aacgcgggca agcgcgacac ccacgtgctg gccgacggct ggacggtcgt cacgaaggac cattcgctgt ccgcgcaatg ggagcacatg gtcgtcgtga cggaaacggg tttcgaggtg ctcaccgagg acgcgaagcc ccaggcgttc tcggcgcttc cgggcacgca cgctgcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at