BuceA.00038.a

NADH-quinone oxidoreductase subunit D (EC 1.6.99.5) (NADH dehydrogenase I subunit D) (NDH-1 subunit D)

CENTER ID: BuceA.00038.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00038.a.B3.GE35010 C-term domain 150 417
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2441
RefSeq: YP_002231465.1
UniProt: B4E5L9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEIKNYTLN FGPQHPAAHG VLRLVLELDG EVIQRADPHI GLLHRATEKL AESKTFIQSV PYMDRLDYVS MMVNEHGYVL AIEKLLGIEV PERAQYIRVL FDEITRVLNH LMWIGAHALD VGAMAVFLYA FREREDLMDV YEAVSGARMH AAYYRPGGVY RDLPDAMPQY KASKIRNEKA LAKMNEARSG SVLDFIDDFF TRFPKCVDEY ETLLTDNRIW KQRLVGIGVV SPERALQMGL TGPMLRGSGI AWDLRKKQPY EVYDRMDFDV PVGVNGDCYD RYLVRVEEMR QSIRIAKQCI EWLRKNPGPV MTDNHKVAPP SRVGMKTNME DLIHHFKLFT EGFHVPEGEA YAAVEHPKGE FGIYLVSDGA NKPYRLKIRA PGFAHLASLD EMARGHMIAD AVTIIGTQDI VFGEIDR
NT Sequence
atggcagaaa tcaagaacta cacgctcaac ttcggcccgc agcacccggc agcgcacggc gtgctgcgcc tcgtgctcga gctcgacggc gaagtcatcc agcgtgccga tccgcacatc ggcctgctgc accgcgcgac ggaaaagctc gcggaatcca agaccttcat ccagtccgtg ccgtacatgg accgtctcga ctacgtgtcg atgatggtca acgagcacgg ctacgtgctc gcgatcgaaa agctgctcgg catcgaagtg ccggagcgcg cgcagtacat ccgcgtgctg ttcgacgaaa tcacgcgcgt gctgaaccac ctgatgtgga tcggcgcgca cgcactcgac gtcggcgcga tggccgtgtt cctgtacgcg ttccgcgagc gcgaagacct gatggacgtg tacgaagcgg tgtccggcgc ccggatgcac gcggcctact atcgtccggg cggcgtctat cgcgatctgc ctgacgcaat gccgcaatac aaggcgtcga agattcgcaa cgagaaggcg ctcgcgaaga tgaacgaagc gcgcagtggt tcggtgctcg acttcatcga cgatttcttc acgcgcttcc cgaagtgcgt cgacgaatat gaaacgctgc tgaccgacaa ccggatctgg aaacagcgtc tggtcgggat cggcgtggtg agcccggaac gtgcgctgca gatgggcctg acgggcccga tgctgcgcgg ctcgggcatc gcgtgggacc tgcgcaagaa gcagccctac gaagtgtacg atcggatgga tttcgacgtg ccggtcggcg tgaacggcga ttgctacgac cgctatctgg tgcgcgtcga agaaatgcgc cagtcgatcc gcatcgcgaa acagtgtatt gagtggctcc gcaagaatcc gggcccggtg atgacggaca atcacaaggt tgccccgccg tcgcgcgtcg gcatgaagac caacatggaa gacttgattc accacttcaa gctcttcacc gaaggtttcc atgtgccgga aggcgaagcg tacgcggcgg tcgagcatcc gaagggtgaa ttcggcatct acctcgtgtc ggacggcgcc aacaagccgt atcgcctcaa gattcgcgca ccgggtttcg cgcacctggc gtcgctcgac gaaatggcgc gcggtcacat gatcgccgac gccgtcacga tcatcggtac gcaggacatc gtgttcggcg aaatcgatcg ctaa
Details for BuceA.00038.a.B3.GE35010
HARVESTED ON: 3/19/2012
SEQUENCED ON: 3/22/2012
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 97
Validated AA Sequence
MHAAYYRPGG VYRDLPDAMP QYKASKIRNE KALAKMNEAR SGSVLDFIDD FFTRFPKCVD EYETLLTDNR IWKQRLVGIG VVSPERALQM GLTGPMLRGS GIAWDLRKKQ PYEVYDRMDF DVPVGVNGDC YDRYLVRVEE MRQSIRIAKQ CIEWLRKNPG PVMTDNHKVA PPSRVGMKTN MEDLIHHFKL FTEGFHVPEG EAYAAVEHPK GEFGIYLVSD GAXKPYRLKI RAPGFAHLAS LDEMARGHMI ADAVTIIGTX XIVFGEIDR
Validated NT Sequence
anattttgtn nnntttaaga nggagatata ccntggctca ccaccaccac caccatatgc acgcggccta ctatcgtccg ggcggcgtct atcgcgatct gcctgacgca atgccgcaat acaaggcgtc gaagattcgc aacgagaagg cgctcgcgaa gatgaacgaa gcgcgcagtg gttcggtgct cgacttcatc gacgatttct tcacgcgctt cccgaagtgc gtcgacgaat atgaaacgct gctgaccgac aaccggatct ggaaacagcg tctggtcggg atcggcgtgg tgagcccgga acgtgcgctg cagatgggcc tgacgggccc gatgctgcgc ggctcgggca tcgcgtggga cctgcgcaag aagcagccct acgaagtgta cgatcggatg gatttcgacg tgccggtcgg cgtgaacggc gattgctacg accgctatct ggtgcgcgtc gaagaaatgc gccagtcgat ccgcatcgcg aaacagtgta ttgagtggct ccgcaagaat ccgggcccgg tgatgacgga caatcacaag gttgccccgc cgtcgcgcgt cggcatgaag accaacatgg aagacttgat tcaccacttc aagctcttca ccgaaggttt ccatgtgccg gaaggcgaag cgtacgcggc ggtcgagcat ccgaagggtg aattcggcat ctacctcgtg tcggacggcg ccnacaagcc gtatcgcctc aagattcgcg caccgggttt cgcgcacctg gcgtcgctcg acgaaatggc gcgcggtcac atgatcgccg acgccgtcac gatcatcggt acgcnnnaca tcgtgttcgg cgaaatcgat cgctaagtga gtaanatagg atccnnctgn nnnnnccccc nnaannnnnn nntngcnnnn natgcnnnga cnnnnnnnga ngngngngng ntcnnnnngn nnnnnnnnnn nntnnnnnnn nnctgnnnna nnnnnnnnna nnnnnncngn ncancatnnc cnnngccnnn nggcnncnnn ntnnnnnncn gnnntaagta aa
Expected Protein Sequence
MAHHHHHHMH AAYYRPGGVY RDLPDAMPQY KASKIRNEKA LAKMNEARSG SVLDFIDDFF TRFPKCVDEY ETLLTDNRIW KQRLVGIGVV SPERALQMGL TGPMLRGSGI AWDLRKKQPY EVYDRMDFDV PVGVNGDCYD RYLVRVEEMR QSIRIAKQCI EWLRKNPGPV MTDNHKVAPP SRVGMKTNME DLIHHFKLFT EGFHVPEGEA YAAVEHPKGE FGIYLVSDGA NKPYRLKIRA PGFAHLASLD EMARGHMIAD AVTIIGTQDI VFGEIDR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcacg cggcctacta tcgtccgggc ggcgtctatc gcgatctgcc tgacgcaatg ccgcaataca aggcgtcgaa gattcgcaac gagaaggcgc tcgcgaagat gaacgaagcg cgcagtggtt cggtgctcga cttcatcgac gatttcttca cgcgcttccc gaagtgcgtc gacgaatatg aaacgctgct gaccgacaac cggatctgga aacagcgtct ggtcgggatc ggcgtggtga gcccggaacg tgcgctgcag atgggcctga cgggcccgat gctgcgcggc tcgggcatcg cgtgggacct gcgcaagaag cagccctacg aagtgtacga tcggatggat ttcgacgtgc cggtcggcgt gaacggcgat tgctacgacc gctatctggt gcgcgtcgaa gaaatgcgcc agtcgatccg catcgcgaaa cagtgtattg agtggctccg caagaatccg ggcccggtga tgacggacaa tcacaaggtt gccccgccgt cgcgcgtcgg catgaagacc aacatggaag acttgattca ccacttcaag ctcttcaccg aaggtttcca tgtgccggaa ggcgaagcgt acgcggcggt cgagcatccg aagggtgaat tcggcatcta cctcgtgtcg gacggcgcca acaagccgta tcgcctcaag attcgcgcac cgggtttcgc gcacctggcg tcgctcgacg aaatggcgcg cggtcacatg atcgccgacg ccgtcacgat catcggtacg caggacatcg tgttcggcga aatcgatcgc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat