BuceA.00036.a

Phosphoribosylaminoimidazole carboxylase ATPase subunit

CENTER ID: BuceA.00036.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00036.a.A1.GE33163 Full length( BuceA.00036.a ) 1 398
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00036.a.A1.PW33773 Full length( BuceA.00036.a ) 1 398

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2935
RefSeq: YP_002231938.1
UniProt: B4EA20

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTATPDSVSP ILPGAWLGMV GGGQLGRMFC FAAQSMGYRV AVLDPDPTSP AGAVADRHLR AAYDDEAALA ELAGLCEAVS TEFENVPAAS LDFLARTTFV APAGRCVAVA QDRIAEKRFI AASGVPVAPH VVIESAAALA ALDDAALDAV LPGILKTARL GYDGKGQVRV ATAQEARDAH AALGGVPCVL EKRLPLKYEV SALIARGADG RSAAFPLAQN VHHNGILALT VVPAPAADAA RVEEAQQAAI RIADTLGYVG VLCVEFFVLE DGSFVANEMA PRPHNSGHYT VDACATSQFE QQVRAMTRMP LGNPRQHSPA AMLNILGDVW FPNGAAADAV TPPWDTVAAM PAAHLHLYGK EEARVGRKMG HVNFTAETRD EAVAAATACA QLLRVPLD
NT Sequence
ATGACCGCAA CTCCTGATTC CGTTTCCCCG ATCCTGCCCG GCGCGTGGCT GGGCATGGTC GGCGGTGGCC AGCTCGGCCG CATGTTCTGC TTTGCCGCCC AGTCGATGGG CTATCGCGTC GCCGTGCTCG ATCCCGATCC GACGAGCCCC GCCGGCGCGG TCGCCGATCG CCACCTGCGC GCGGCGTACG ACGACGAAGC TGCGCTCGCC GAACTGGCCG GCCTGTGCGA GGCCGTATCG ACGGAGTTCG AGAACGTGCC GGCCGCGAGC CTCGATTTCC TCGCGCGCAC GACGTTCGTC GCGCCGGCCG GCCGCTGCGT CGCGGTCGCG CAGGACCGGA TCGCGGAGAA GCGTTTCATC GCGGCATCGG GCGTGCCGGT CGCGCCGCAC GTCGTGATCG AATCGGCGGC GGCGCTCGCC GCGCTTGACG ATGCGGCGCT CGACGCGGTG CTGCCGGGCA TCCTGAAGAC GGCCCGCCTC GGGTACGACG GCAAGGGCCA GGTGCGGGTC GCCACCGCGC AGGAAGCGCG CGACGCGCAT GCCGCGCTCG GCGGCGTGCC GTGCGTGCTG GAGAAGCGCC TGCCGCTGAA ATACGAAGTG TCGGCGTTGA TCGCGCGCGG CGCGGACGGC CGTTCGGCCG CCTTCCCGCT CGCGCAGAAC GTGCATCACA ACGGCATCCT CGCGCTGACG GTCGTGCCGG CGCCGGCCGC CGATGCGGCC CGCGTCGAGG AAGCGCAGCA GGCGGCGATC CGGATCGCCG ATACGCTCGG CTACGTCGGC GTGCTGTGCG TCGAATTCTT CGTGCTGGAG GACGGTTCGT TCGTCGCGAA CGAAATGGCG CCGCGCCCGC ACAACTCCGG CCACTATACG GTCGATGCGT GCGCAACGAG CCAGTTCGAG CAGCAGGTGC GCGCGATGAC GCGCATGCCG CTCGGCAACC CGCGCCAGCA TTCGCCGGCC GCGATGCTGA ACATTCTCGG CGACGTGTGG TTCCCCAACG GTGCGGCGGC CGATGCCGTC ACGCCGCCGT GGGACACGGT CGCCGCGATG CCGGCCGCCC ACCTGCACCT GTACGGCAAG GAAGAAGCAC GTGTCGGCCG CAAGATGGGC CACGTGAACT TCACGGCCGA GACGCGCGAC GAAGCCGTCG CCGCTGCCAC CGCGTGCGCG CAACTGCTGC GCGTGCCGCT CGAC
Details for BuceA.00036.a.A1.GE33163
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MTATPDSVSP ILPGAWLGMV GGGQLGRMFC FAAQSMGYRV AVLDPDPTSP AGAVADRHLR AAYDDEAALA ELAGLCEAVS TEFENVPAAS LDFLARTTFV APAGRCVAVA QDRIAEKRFI AASGVPVAPH VVIESAAALA ALDDAALDAV LPGILKTARL GYDGKGQVRV ATAQEARDAH AALGGVPCVL EKRLPLKYEV SALIARGADG RSAAFPLAQN VHHNGILALT VVPAPAADAA RVEEAQQAAI RIADTLGYVG VLCVEFFVLE DGSFVANEMA PRPHNSGHYT VDACATSQFE QQVRAMTRMP LGNPRQHSPA AMLNILGDVW FPNGAAADAV TPPWDTVAAM PAAHLHLYGK EEARVGRKMG HVNFTAETRD EAVAAATACA QLLRVPL
Validated NT Sequence
tcgagcggca cgcgcagcag ttgcgcgcac gcggtggcag cggcgacggc ttcgtcgcgc gtctcggccg tgaagttcac gtggcccatc ttgcggccga cacgtgcttc ttccttgccg tacaggtgca ggtgggcggc cggcatcgcg gcgaccgtgt cccacggcgg cgtgacggca tcggccgccg caccgttggg gaaccacacg tcgccgagaa tgttcagcat cgcggccggc gaatgctggc gcgggttgcc gagcggcatg cgcgtcatcg cgcgcacctg ctgctcgaac tggctcgttg cgcacgcatc gaccgtatag tggccggagt tgtgcgggcg cggcgccatt tcgttcgcga cgaacgaacc gtcctccagc acgaagaatt cgacgcacag cacgccgacg tagccgagcg tatcggcgat ccggatcgcc gcctgctgcg cttcctcgac gcgggccgca tcggcggccg gcgccggcac gaccgtcagc gcgaggatgc cgttgtgatg cacgttctgc gcgagcggga aggcggccga acggccgtcc gcgccgcgcg cgatcaacgc cgacacttcg tatttcagcg gcaggcgctt ctccagcacg cacggcacgc cgccgagcgc ggcatgcgcg tcgcgcgctt cctgcgcggt ggcgacccgc acctggccct tgccgtcgta cccgaggcgg gccgtcttca ggatgcccgg cagcaccgcg tcgagcgccg catcgtcaag cgcggcgagc gccgccgccg attcgatcac gacgtgcggc gcgaccggca cgcccgatgc cgcgatgaaa cgcttctccg cgatccggtc ctgcgcgacc gcgacgcagc ggccggccgg cgcgacgaac gtcgtgcgcg cgaggaaatc gaggctcgcg gccggcacgt tctcgaactc cgtcgatacg gcctcgcaca ggccggccag ttcggcgagc gcagcttcgt cgtcgtacgc cgcgcgcagg tggcgatcgg cgaccgcgcc ggcggggctc gtcggatcgg gatcgagcac ggcgacgcga tagcccatcg actgggcggc aaagcagaac atgcggccga gctggccacc gccgaccatg cccagccacg cgccgggcag gatcggggaa acggaatcag gagttgcggt catcgaacca ggaccctggg tctgagcttc cagggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTATPDSVS PILPGAWLGM VGGGQLGRMF CFAAQSMGYR VAVLDPDPTS PAGAVADRHL RAAYDDEAAL AELAGLCEAV STEFENVPAA SLDFLARTTF VAPAGRCVAV AQDRIAEKRF IAASGVPVAP HVVIESAAAL AALDDAALDA VLPGILKTAR LGYDGKGQVR VATAQEARDA HAALGGVPCV LEKRLPLKYE VSALIARGAD GRSAAFPLAQ NVHHNGILAL TVVPAPAADA ARVEEAQQAA IRIADTLGYV GVLCVEFFVL EDGSFVANEM APRPHNSGHY TVDACATSQF EQQVRAMTRM PLGNPRQHSP AAMLNILGDV WFPNGAAADA VTPPWDTVAA MPAAHLHLYG KEEARVGRKM GHVNFTAETR DEAVAAATAC AQLLRVPLD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgcaact cctgattccg tttccccgat cctgcccggc gcgtggctgg gcatggtcgg cggtggccag ctcggccgca tgttctgctt tgccgcccag tcgatgggct atcgcgtcgc cgtgctcgat cccgatccga cgagccccgc cggcgcggtc gccgatcgcc acctgcgcgc ggcgtacgac gacgaagctg cgctcgccga actggccggc ctgtgcgagg ccgtatcgac ggagttcgag aacgtgccgg ccgcgagcct cgatttcctc gcgcgcacga cgttcgtcgc gccggccggc cgctgcgtcg cggtcgcgca ggaccggatc gcggagaagc gtttcatcgc ggcatcgggc gtgccggtcg cgccgcacgt cgtgatcgaa tcggcggcgg cgctcgccgc gcttgacgat gcggcgctcg acgcggtgct gccgggcatc ctgaagacgg cccgcctcgg gtacgacggc aagggccagg tgcgggtcgc caccgcgcag gaagcgcgcg acgcgcatgc cgcgctcggc ggcgtgccgt gcgtgctgga gaagcgcctg ccgctgaaat acgaagtgtc ggcgttgatc gcgcgcggcg cggacggccg ttcggccgcc ttcccgctcg cgcagaacgt gcatcacaac ggcatcctcg cgctgacggt cgtgccggcg ccggccgccg atgcggcccg cgtcgaggaa gcgcagcagg cggcgatccg gatcgccgat acgctcggct acgtcggcgt gctgtgcgtc gaattcttcg tgctggagga cggttcgttc gtcgcgaacg aaatggcgcc gcgcccgcac aactccggcc actatacggt cgatgcgtgc gcaacgagcc agttcgagca gcaggtgcgc gcgatgacgc gcatgccgct cggcaacccg cgccagcatt cgccggccgc gatgctgaac attctcggcg acgtgtggtt ccccaacggt gcggcggccg atgccgtcac gccgccgtgg gacacggtcg ccgcgatgcc ggccgcccac ctgcacctgt acggcaagga agaagcacgt gtcggccgca agatgggcca cgtgaacttc acggccgaga cgcgcgacga agccgtcgcc gctgccaccg cgtgcgcgca actgctgcgc gtgccgctcg actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BuceA.00036.a.A1.PW33773
PURIFICATION DATe: 11/19/2011
CONCENTRATION: 33.57mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 16
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MTATPDSVSP ILPGAWLGMV GGGQLGRMFC FAAQSMGYRV AVLDPDPTSP AGAVADRHLR AAYDDEAALA ELAGLCEAVS TEFENVPAAS LDFLARTTFV APAGRCVAVA QDRIAEKRFI AASGVPVAPH VVIESAAALA ALDDAALDAV LPGILKTARL GYDGKGQVRV ATAQEARDAH AALGGVPCVL EKRLPLKYEV SALIARGADG RSAAFPLAQN VHHNGILALT VVPAPAADAA RVEEAQQAAI RIADTLGYVG VLCVEFFVLE DGSFVANEMA PRPHNSGHYT VDACATSQFE QQVRAMTRMP LGNPRQHSPA AMLNILGDVW FPNGAAADAV TPPWDTVAAM PAAHLHLYGK EEARVGRKMG HVNFTAETRD EAVAAATACA QLLRVPL
Validated NT Sequence
tcgagcggca cgcgcagcag ttgcgcgcac gcggtggcag cggcgacggc ttcgtcgcgc gtctcggccg tgaagttcac gtggcccatc ttgcggccga cacgtgcttc ttccttgccg tacaggtgca ggtgggcggc cggcatcgcg gcgaccgtgt cccacggcgg cgtgacggca tcggccgccg caccgttggg gaaccacacg tcgccgagaa tgttcagcat cgcggccggc gaatgctggc gcgggttgcc gagcggcatg cgcgtcatcg cgcgcacctg ctgctcgaac tggctcgttg cgcacgcatc gaccgtatag tggccggagt tgtgcgggcg cggcgccatt tcgttcgcga cgaacgaacc gtcctccagc acgaagaatt cgacgcacag cacgccgacg tagccgagcg tatcggcgat ccggatcgcc gcctgctgcg cttcctcgac gcgggccgca tcggcggccg gcgccggcac gaccgtcagc gcgaggatgc cgttgtgatg cacgttctgc gcgagcggga aggcggccga acggccgtcc gcgccgcgcg cgatcaacgc cgacacttcg tatttcagcg gcaggcgctt ctccagcacg cacggcacgc cgccgagcgc ggcatgcgcg tcgcgcgctt cctgcgcggt ggcgacccgc acctggccct tgccgtcgta cccgaggcgg gccgtcttca ggatgcccgg cagcaccgcg tcgagcgccg catcgtcaag cgcggcgagc gccgccgccg attcgatcac gacgtgcggc gcgaccggca cgcccgatgc cgcgatgaaa cgcttctccg cgatccggtc ctgcgcgacc gcgacgcagc ggccggccgg cgcgacgaac gtcgtgcgcg cgaggaaatc gaggctcgcg gccggcacgt tctcgaactc cgtcgatacg gcctcgcaca ggccggccag ttcggcgagc gcagcttcgt cgtcgtacgc cgcgcgcagg tggcgatcgg cgaccgcgcc ggcggggctc gtcggatcgg gatcgagcac ggcgacgcga tagcccatcg actgggcggc aaagcagaac atgcggccga gctggccacc gccgaccatg cccagccacg cgccgggcag gatcggggaa acggaatcag gagttgcggt catcgaacca ggaccctggg tctgagcttc cagggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTATPDSVS PILPGAWLGM VGGGQLGRMF CFAAQSMGYR VAVLDPDPTS PAGAVADRHL RAAYDDEAAL AELAGLCEAV STEFENVPAA SLDFLARTTF VAPAGRCVAV AQDRIAEKRF IAASGVPVAP HVVIESAAAL AALDDAALDA VLPGILKTAR LGYDGKGQVR VATAQEARDA HAALGGVPCV LEKRLPLKYE VSALIARGAD GRSAAFPLAQ NVHHNGILAL TVVPAPAADA ARVEEAQQAA IRIADTLGYV GVLCVEFFVL EDGSFVANEM APRPHNSGHY TVDACATSQF EQQVRAMTRM PLGNPRQHSP AAMLNILGDV WFPNGAAADA VTPPWDTVAA MPAAHLHLYG KEEARVGRKM GHVNFTAETR DEAVAAATAC AQLLRVPLD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGCAACT CCTGATTCCG TTTCCCCGAT CCTGCCCGGC GCGTGGCTGG GCATGGTCGG CGGTGGCCAG CTCGGCCGCA TGTTCTGCTT TGCCGCCCAG TCGATGGGCT ATCGCGTCGC CGTGCTCGAT CCCGATCCGA CGAGCCCCGC CGGCGCGGTC GCCGATCGCC ACCTGCGCGC GGCGTACGAC GACGAAGCTG CGCTCGCCGA ACTGGCCGGC CTGTGCGAGG CCGTATCGAC GGAGTTCGAG AACGTGCCGG CCGCGAGCCT CGATTTCCTC GCGCGCACGA CGTTCGTCGC GCCGGCCGGC CGCTGCGTCG CGGTCGCGCA GGACCGGATC GCGGAGAAGC GTTTCATCGC GGCATCGGGC GTGCCGGTCG CGCCGCACGT CGTGATCGAA TCGGCGGCGG CGCTCGCCGC GCTTGACGAT GCGGCGCTCG ACGCGGTGCT GCCGGGCATC CTGAAGACGG CCCGCCTCGG GTACGACGGC AAGGGCCAGG TGCGGGTCGC CACCGCGCAG GAAGCGCGCG ACGCGCATGC CGCGCTCGGC GGCGTGCCGT GCGTGCTGGA GAAGCGCCTG CCGCTGAAAT ACGAAGTGTC GGCGTTGATC GCGCGCGGCG CGGACGGCCG TTCGGCCGCC TTCCCGCTCG CGCAGAACGT GCATCACAAC GGCATCCTCG CGCTGACGGT CGTGCCGGCG CCGGCCGCCG ATGCGGCCCG CGTCGAGGAA GCGCAGCAGG CGGCGATCCG GATCGCCGAT ACGCTCGGCT ACGTCGGCGT GCTGTGCGTC GAATTCTTCG TGCTGGAGGA CGGTTCGTTC GTCGCGAACG AAATGGCGCC GCGCCCGCAC AACTCCGGCC ACTATACGGT CGATGCGTGC GCAACGAGCC AGTTCGAGCA GCAGGTGCGC GCGATGACGC GCATGCCGCT CGGCAACCCG CGCCAGCATT CGCCGGCCGC GATGCTGAAC ATTCTCGGCG ACGTGTGGTT CCCCAACGGT GCGGCGGCCG ATGCCGTCAC GCCGCCGTGG GACACGGTCG CCGCGATGCC GGCCGCCCAC CTGCACCTGT ACGGCAAGGA AGAAGCACGT GTCGGCCGCA AGATGGGCCA CGTGAACTTC ACGGCCGAGA CGCGCGACGA AGCCGTCGCC GCTGCCACCG CGTGCGCGCA ACTGCTGCGC GTGCCGCTCG ACTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA