SSGCID: Target Page

BuceA.00027.f

Putative acyl-CoA dehydrogenase

CENTER ID:	BuceA.00027.f
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00027.f.B1.GE36517	Full length( BuceA.00027.f )		1	384

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00027.f.B1.PS01791	Full length( BuceA.00027.f )		1	384

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.6521
RefSeq:	YP_002235299.1
UniProt:	B4EKW7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNFELELEHR MIRDLVARFV KDELIPLEAA VMAREADGQR FGLLVDERAH LDERSRQLGL WGLDAPLDVG GSDLPAVAMV GVNEAIGTTV TPYILPPDSP NLRMLCDAAD DAQRARYLDP YARGATRAAM AISEPGAGSD PGALSTTAVH DGDGWVLNGR KIWISHAADA DWTIVMALTD RSKGKRGGMS AFIVDRDTPG FIIERRIPMI GGLSTYEIVL EDCRIPAAQL LGPEGAGFTP MQARLANRRL EMAAWCIGRA ERAVALLCDH AKQRTTFGVP LAERQAVQWW VADALTQIHA CRLMTYEAAA RVDAGQDART QISMVKVFAT EMAWTVIDHA MQTLGAMGMT KEMPLQQMAS EARLMRIFEG PTEVHRWVVA RSVL

NT Sequence

ATGAATTTCG AACTCGAACT CGAACACCGG ATGATCCGGG ATCTCGTCGC GCGTTTCGTG AAGGACGAAC TGATTCCGCT CGAAGCGGCG GTGATGGCGC GCGAGGCCGA CGGCCAGCGC TTCGGGCTGC TCGTCGACGA ACGTGCGCAC CTCGACGAGC GCTCCCGGCA ACTCGGCCTG TGGGGACTCG ACGCGCCGCT CGACGTGGGC GGCTCGGATC TGCCGGCCGT CGCGATGGTC GGCGTGAACG AGGCGATCGG CACGACCGTC ACCCCGTACA TCCTGCCGCC CGACTCGCCG AACCTGCGGA TGCTGTGCGA TGCGGCCGAC GATGCGCAGC GCGCGCGCTA TCTCGACCCG TACGCACGCG GCGCCACGCG TGCCGCGATG GCGATCTCCG AGCCGGGCGC CGGCTCCGAC CCGGGCGCGC TGTCGACGAC CGCCGTGCAC GACGGCGACG GCTGGGTGCT GAACGGCCGG AAGATCTGGA TCAGCCACGC GGCCGATGCC GACTGGACGA TCGTGATGGC GCTGACCGAT CGCAGCAAGG GCAAGCGCGG CGGCATGTCG GCGTTCATCG TCGATCGCGA CACGCCGGGT TTCATCATCG AACGCCGGAT TCCGATGATC GGCGGGCTGT CGACCTACGA AATCGTGCTG GAAGACTGCC GCATTCCCGC CGCGCAACTG CTTGGCCCGG AAGGCGCCGG CTTCACGCCG ATGCAGGCCC GGCTCGCGAA CCGGCGGCTG GAGATGGCCG CGTGGTGCAT CGGCCGCGCC GAGCGCGCGG TCGCGCTGCT GTGCGACCAT GCGAAACAGC GCACGACCTT CGGCGTGCCG CTCGCGGAGC GTCAGGCGGT CCAGTGGTGG GTCGCCGACG CGCTCACGCA GATCCACGCA TGCCGCCTGA TGACCTACGA AGCGGCCGCG CGCGTGGATG CCGGGCAGGA TGCCCGCACG CAGATCTCGA TGGTCAAGGT GTTCGCGACC GAAATGGCGT GGACCGTGAT CGATCATGCG ATGCAGACGC TCGGCGCGAT GGGCATGACG AAGGAAATGC CGTTGCAGCA GATGGCCAGC GAAGCGCGGC TGATGCGCAT CTTCGAAGGC CCGACCGAGG TGCACCGTTG GGTCGTCGCG CGCAGCGTGC TC

Details for BuceA.00027.f.B1.GE36517

HARVESTED ON:	1/9/2013
SEQUENCED ON:	1/7/2013
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MIRDLVARFV KDELIPLEAA VMAREADGQR FGLLVDERAH LDERSRQLGL WGLDAPLDVG GSDLPAVAMV GVNEAIGTTV TPYILPPDSP NLRMLCDAAD DAQRARYLDP YARGATRAAM AISEPGAGSD PGALSTTAVH DGDGWVLNGR KIWISHAADA DWTIVMALTD RSKGKRGGMS AFIVDRDTPG FIIERRIPMI GGLSTYEIVL EDCRIPAAQL LGPEGAGFTP MQARLANRRL EMAAWCIGRA ERAVALLCDH AKQRTTFGVP LAERQAVQWW VADALTQIHA CRLMTYEAAA RVDAGQDART QISMVKVFAT EMAWTVIDHA MQTLGAMGMT KEMPLQQMAS EARLMRIFEG PTEVH

Validated NT Sequence

acggtgcacc tcggtcgggc cttcgaagat gcgcatcagc cgcgcttcgc tggccatctg ctgcaacggc atttccttcg tcatgcccat cgcgccgagc gtctgcatcg catgatcgat cacggtccac gccatttcgg tcgcgaacac cttgaccatc gagatctgcg tgcgggcatc ctgcccggca tccacgcgcg cggccgcttc gtaggtcatc aggcggcatg cgtggatctg cgtgagcgcg tcggcgaccc accactggac cgcctgacgc tccgcgagcg gcacgccgaa ggtcgtgcgc tgtttcgcat ggtcgcacag cagcgcgacc gcgcgctcgg cgcggccgat gcaccacgcg gccatctcca gccgccggtt cgcgagccgg gcctgcatcg gcgtgaagcc ggcgccttcc gggccaagca gttgcgcggc gggaatgcgg cagtcttcca gcacgatttc gtaggtcgac agcccgccga tcatcggaat ccggcgttcg atgatgaaac ccggcgtgtc gcgatcgacg atgaacgccg acatgccgcc gcgcttgccc ttgctgcgat cggtcagcgc catcacgatc gtccagtcgg catcggccgc gtggctgatc cagatcttcc ggccgttcag cacccagccg tcgccgtcgt gcacggcggt cgtcgacagc gcgcccgggt cggagccggc gcccggctcg gagatcgcca tcgcggcacg cgtggcgccg cgtgcgtacg ggtcgagata gcgcgcgcgc tgcgcatcgt cggccgcatc gcacagcatc cgcaggttcg gcgagtcggg cggcaggatg tacggggtga cggtcgtgcc gatcgcctcg ttcacgccga ccatcgcgac ggccggcaga tccgagccgc ccacgtcgag cggcgcgtcg agtccccaca ggccgagttg ccgggagcgc tcgtcgaggt gcgcacgttc gtcgacgagc agcccgaagc gctggccgtc ggcctcgcgc gccatcaccg ccgcttcgag cggaatcagt tcgtccttca cgaaacgcgc gacgagatcc cggatcatcc ggtgttcgag ttcgagttcg aaa

Expected Protein Sequence

MAHHHHHHMN FELELEHRMI RDLVARFVKD ELIPLEAAVM AREADGQRFG LLVDERAHLD ERSRQLGLWG LDAPLDVGGS DLPAVAMVGV NEAIGTTVTP YILPPDSPNL RMLCDAADDA QRARYLDPYA RGATRAAMAI SEPGAGSDPG ALSTTAVHDG DGWVLNGRKI WISHAADADW TIVMALTDRS KGKRGGMSAF IVDRDTPGFI IERRIPMIGG LSTYEIVLED CRIPAAQLLG PEGAGFTPMQ ARLANRRLEM AAWCIGRAER AVALLCDHAK QRTTFGVPLA ERQAVQWWVA DALTQIHACR LMTYEAAARV DAGQDARTQI SMVKVFATEM AWTVIDHAMQ TLGAMGMTKE MPLQQMASEA RLMRIFEGPT EVHRWVVARS VL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt tcgaactcga actcgaacac cggatgatcc gggatctcgt cgcgcgtttc gtgaaggacg aactgattcc gctcgaagcg gcggtgatgg cgcgcgaggc cgacggccag cgcttcgggc tgctcgtcga cgaacgtgcg cacctcgacg agcgctcccg gcaactcggc ctgtggggac tcgacgcgcc gctcgacgtg ggcggctcgg atctgccggc cgtcgcgatg gtcggcgtga acgaggcgat cggcacgacc gtcaccccgt acatcctgcc gcccgactcg ccgaacctgc ggatgctgtg cgatgcggcc gacgatgcgc agcgcgcgcg ctatctcgac ccgtacgcac gcggcgccac gcgtgccgcg atggcgatct ccgagccggg cgccggctcc gacccgggcg cgctgtcgac gaccgccgtg cacgacggcg acggctgggt gctgaacggc cggaagatct ggatcagcca cgcggccgat gccgactgga cgatcgtgat ggcgctgacc gatcgcagca agggcaagcg cggcggcatg tcggcgttca tcgtcgatcg cgacacgccg ggtttcatca tcgaacgccg gattccgatg atcggcgggc tgtcgaccta cgaaatcgtg ctggaagact gccgcattcc cgccgcgcaa ctgcttggcc cggaaggcgc cggcttcacg ccgatgcagg cccggctcgc gaaccggcgg ctggagatgg ccgcgtggtg catcggccgc gccgagcgcg cggtcgcgct gctgtgcgac catgcgaaac agcgcacgac cttcggcgtg ccgctcgcgg agcgtcaggc ggtccagtgg tgggtcgccg acgcgctcac gcagatccac gcatgccgcc tgatgaccta cgaagcggcc gcgcgcgtgg atgccgggca ggatgcccgc acgcagatct cgatggtcaa ggtgttcgcg accgaaatgg cgtggaccgt gatcgatcat gcgatgcaga cgctcggcgc gatgggcatg acgaaggaaa tgccgttgca gcagatggcc agcgaagcgc ggctgatgcg catcttcgaa ggcccgaccg aggtgcaccg ttgggtcgtc gcgcgcagcg tgctctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for BuceA.00027.f.B1.PS01791

PURIFICATION DATe:	4/2/2013
CONCENTRATION:	10mg/ml
OBSERVED MW:	41kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATT TCGAACTCGA ACTCGAACAC CGGATGATCC GGGATCTCGT CGCGCGTTTC GTGAAGGACG AACTGATTCC GCTCGAAGCG GCGGTGATGG CGCGCGAGGC CGACGGCCAG CGCTTCGGGC TGCTCGTCGA CGAACGTGCG CACCTCGACG AGCGCTCCCG GCAACTCGGC CTGTGGGGAC TCGACGCGCC GCTCGACGTG GGCGGCTCGG ATCTGCCGGC CGTCGCGATG GTCGGCGTGA ACGAGGCGAT CGGCACGACC GTCACCCCGT ACATCCTGCC GCCCGACTCG CCGAACCTGC GGATGCTGTG CGATGCGGCC GACGATGCGC AGCGCGCGCG CTATCTCGAC CCGTACGCAC GCGGCGCCAC GCGTGCCGCG ATGGCGATCT CCGAGCCGGG CGCCGGCTCC GACCCGGGCG CGCTGTCGAC GACCGCCGTG CACGACGGCG ACGGCTGGGT GCTGAACGGC CGGAAGATCT GGATCAGCCA CGCGGCCGAT GCCGACTGGA CGATCGTGAT GGCGCTGACC GATCGCAGCA AGGGCAAGCG CGGCGGCATG TCGGCGTTCA TCGTCGATCG CGACACGCCG GGTTTCATCA TCGAACGCCG GATTCCGATG ATCGGCGGGC TGTCGACCTA CGAAATCGTG CTGGAAGACT GCCGCATTCC CGCCGCGCAA CTGCTTGGCC CGGAAGGCGC CGGCTTCACG CCGATGCAGG CCCGGCTCGC GAACCGGCGG CTGGAGATGG CCGCGTGGTG CATCGGCCGC GCCGAGCGCG CGGTCGCGCT GCTGTGCGAC CATGCGAAAC AGCGCACGAC CTTCGGCGTG CCGCTCGCGG AGCGTCAGGC GGTCCAGTGG TGGGTCGCCG ACGCGCTCAC GCAGATCCAC GCATGCCGCC TGATGACCTA CGAAGCGGCC GCGCGCGTGG ATGCCGGGCA GGATGCCCGC ACGCAGATCT CGATGGTCAA GGTGTTCGCG ACCGAAATGG CGTGGACCGT GATCGATCAT GCGATGCAGA CGCTCGGCGC GATGGGCATG ACGAAGGAAA TGCCGTTGCA GCAGATGGCC AGCGAAGCGC GGCTGATGCG CATCTTCGAA GGCCCGACCG AGGTGCACCG TTGGGTCGTC GCGCGCAGCG TGCTCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT