BuceA.00027.d
Putative acyl-CoA dehydrogenase
| CENTER ID: | BuceA.00027.d |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00027.d.B1.GE36515 | Full length( BuceA.00027.d ) | 1 | 377 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00027.d.B1.PS01772 | Full length( BuceA.00027.d ) | 1 | 377 |
Structures
4N5F
DEPOSITED: 10/9/2013
DETERMINATION: XRay
CLONE: BuceA.00027.d.B1.GE36515
PROTEIN: BuceA.00027.d.B1.PS01772
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127618 |
| PATRIC ID: | fig|216591.5.peg.5976 |
| RefSeq: | YP_002234796.1 |
| UniProt: | B4EGC8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDELYTEDQR MIRDAARAFA TEMLAPNAAQ WDHDAHLPDA IVAQLGELGL LGMIVPQELG GSYTDYVAYA LAMEEVAAGD AACATMMSVH NSVGCGPILG FGTPAQKDRW LADMAAGRVI GAFCLTEPHA GSEANNLRTR AELRDGQWVL NGAKQFVTNG QRAGVAIVFA MTDPEAGKRG ISAFLVPTDT PGFIVGKPEK KMGIRASDTC PITFENCAIP EDNLLGNRGE GLKIALSNLE GGRIGIAAQA LGIARAAFDK ARRYAGERVQ FGKPIAEHQA IQQKLADMAV QINAARLLVH HAAKLRTAGL PCLSEASQAK LFASEMAERV CSDAIQIHGG YGYLVDYEVE RHYRDARITQ IYEGTSEVQR MVIARQL
NT Sequence
ATGGACGAGC TTTACACCGA AGACCAGCGG ATGATCCGCG ATGCGGCGCG CGCTTTTGCC ACCGAGATGC TGGCGCCGAA CGCCGCGCAG TGGGACCACG ACGCGCACCT GCCCGACGCG ATCGTCGCGC AGCTCGGCGA GCTCGGCCTG CTCGGGATGA TCGTGCCGCA GGAGCTGGGC GGCTCGTATA CGGACTACGT GGCGTATGCG CTGGCGATGG AGGAAGTGGC GGCCGGCGAC GCCGCGTGCG CGACGATGAT GAGCGTGCAC AACTCGGTCG GCTGCGGGCC GATCCTCGGC TTCGGCACGC CCGCGCAGAA GGACCGCTGG CTGGCCGACA TGGCGGCCGG TCGCGTGATC GGCGCGTTCT GCCTGACCGA GCCGCATGCC GGTTCCGAAG CGAACAACCT GCGCACGCGG GCCGAGCTGC GCGACGGCCA ATGGGTGCTG AACGGCGCGA AGCAGTTCGT GACCAACGGC CAGCGCGCCG GCGTCGCGAT CGTTTTTGCC ATGACCGACC CGGAAGCCGG CAAGCGCGGC ATTTCCGCGT TCCTGGTGCC GACCGACACG CCGGGCTTCA TCGTCGGCAA GCCCGAGAAG AAGATGGGCA TCCGCGCGTC GGATACGTGC CCGATCACGT TCGAGAACTG CGCGATTCCG GAAGACAACC TGCTCGGCAA TCGCGGCGAA GGGCTGAAGA TCGCGCTGTC GAACCTCGAA GGCGGCCGCA TCGGCATCGC CGCGCAGGCG CTCGGCATCG CGCGCGCCGC GTTCGACAAG GCGCGCCGCT ATGCGGGCGA GCGCGTGCAG TTCGGCAAGC CGATCGCCGA GCATCAGGCG ATCCAGCAGA AGCTCGCCGA CATGGCCGTG CAGATCAACG CCGCGCGCCT GCTCGTGCAT CACGCGGCGA AGCTGCGCAC GGCCGGGCTG CCGTGCCTGT CGGAAGCGTC GCAGGCGAAG CTGTTCGCGT CCGAGATGGC CGAGCGCGTG TGTTCGGATG CGATCCAGAT TCACGGCGGC TACGGCTACC TGGTCGATTA CGAAGTCGAG CGTCACTATC GCGACGCGCG CATCACGCAG ATTTACGAAG GCACCAGCGA AGTGCAACGG ATGGTGATCG CGCGGCAGCT T
Details for BuceA.00027.d.B1.GE36515
| HARVESTED ON: | 1/9/2013 |
| SEQUENCED ON: | 1/7/2013 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 20 |
| PERCENT COVERAGE: | 60 |
Validated AA Sequence
MGAERREAVR DQRPARRRRD RFCHDRPGSR QARHFRVPGA DRHAGLHRRQ AREEDGHPRV GYVPDHVREL RDSGRQPARQ SRRRAEDRAV EPRRRPHRHR RAGARHRARR VRQGAPLCGR ARAVRQADRR ASGDPAEARR HGRADQRRAP ARASRGEAAH GRAAVPVGSV AGEAVRVRDG RARVFGCDPD SRRLRLPGRL RSRASLSRRA HHADLRRHQR SATDGDRAAA LSE
Validated NT Sequence
atcttactca cttaaagctg ccgcgcgatc accatccgtt gcacttcgct ggtgccttcg taaatctgcg tgatgcgcgc gtcgcgatag tgacgctcga cttcgtaatc gaccaggtag ccgtagccgc cgtgaatctg gatcgcatcc gaacacacgc gctcggccat ctcggacgcg aacagcttcg cctgcgacgc ttccgacagg cacggcagcc cggccgtgcg cagcttcgcc gcgtgatgca cgagcaggcg cgcggcgttg atctgcacgg ccatgtcggc gagcttctgc tggatcgcct gatgctcggc gatcggcttg ccgaactgca cgcgctcgcc cgcatagcgg cgcgccttgt cgaacgcggc gcgcgcgatg ccgagcgcct gcgcggcgat gccgatgcgg ccgccttcga ggttcgacag cgcgatcttc agcccttcgc cgcgattgcc gagcaggttg tcttccggaa tcgcgcagtt ctcgaacgtg atcgggcacg tatccgacgc gcggatgccc atcttcttct cgggcttgcc gacgatgaag cccggcgtgt cggtcggcac caggaacgcg gaaatgccgc gcttgccggc ttccgggtcg gtcatggcaa aaacgatcgc gacgccggcg cgctggccgt tggtcacgaa ctgcttcgcg ccgttcagca cccattggcc gtcgcgcagc tcggcccgcg tgcgcangtt gttcgcttcg gaaccggcat gcggctcggt caggcanaac gcgccgatca cgcgaccggn cgcnntgtcg gncanncngc ggtccntctg cgcgggcgtg ccgaancc
Expected Protein Sequence
MAHHHHHHMD ELYTEDQRMI RDAARAFATE MLAPNAAQWD HDAHLPDAIV AQLGELGLLG MIVPQELGGS YTDYVAYALA MEEVAAGDAA CATMMSVHNS VGCGPILGFG TPAQKDRWLA DMAAGRVIGA FCLTEPHAGS EANNLRTRAE LRDGQWVLNG AKQFVTNGQR AGVAIVFAMT DPEAGKRGIS AFLVPTDTPG FIVGKPEKKM GIRASDTCPI TFENCAIPED NLLGNRGEGL KIALSNLEGG RIGIAAQALG IARAAFDKAR RYAGERVQFG KPIAEHQAIQ QKLADMAVQI NAARLLVHHA AKLRTAGLPC LSEASQAKLF ASEMAERVCS DAIQIHGGYG YLVDYEVERH YRDARITQIY EGTSEVQRMV IARQL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacg agctttacac cgaagaccag cggatgatcc gcgatgcggc gcgcgctttt gccaccgaga tgctggcgcc gaacgccgcg cagtgggacc acgacgcgca cctgcccgac gcgatcgtcg cgcagctcgg cgagctcggc ctgctcggga tgatcgtgcc gcaggagctg ggcggctcgt atacggacta cgtggcgtat gcgctggcga tggaggaagt ggcggccggc gacgccgcgt gcgcgacgat gatgagcgtg cacaactcgg tcggctgcgg gccgatcctc ggcttcggca cgcccgcgca gaaggaccgc tggctggccg acatggcggc cggtcgcgtg atcggcgcgt tctgcctgac cgagccgcat gccggttccg aagcgaacaa cctgcgcacg cgggccgagc tgcgcgacgg ccaatgggtg ctgaacggcg cgaagcagtt cgtgaccaac ggccagcgcg ccggcgtcgc gatcgttttt gccatgaccg acccggaagc cggcaagcgc ggcatttccg cgttcctggt gccgaccgac acgccgggct tcatcgtcgg caagcccgag aagaagatgg gcatccgcgc gtcggatacg tgcccgatca cgttcgagaa ctgcgcgatt ccggaagaca acctgctcgg caatcgcggc gaagggctga agatcgcgct gtcgaacctc gaaggcggcc gcatcggcat cgccgcgcag gcgctcggca tcgcgcgcgc cgcgttcgac aaggcgcgcc gctatgcggg cgagcgcgtg cagttcggca agccgatcgc cgagcatcag gcgatccagc agaagctcgc cgacatggcc gtgcagatca acgccgcgcg cctgctcgtg catcacgcgg cgaagctgcg cacggccggg ctgccgtgcc tgtcggaagc gtcgcaggcg aagctgttcg cgtccgagat ggccgagcgc gtgtgttcgg atgcgatcca gattcacggc ggctacggct acctggtcga ttacgaagtc gagcgtcact atcgcgacgc gcgcatcacg cagatttacg aaggcaccag cgaagtgcaa cggatggtga tcgcgcggca gctttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BuceA.00027.d.B1.PS01772
| PURIFICATION DATe: | 3/21/2013 |
| CONCENTRATION: | 39mg/ml |
| OBSERVED MW: | 39kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 20 |
| PERCENT COVERAGE: | 60 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGAERREAVR DQRPARRRRD RFCHDRPGSR QARHFRVPGA DRHAGLHRRQ AREEDGHPRV GYVPDHVREL RDSGRQPARQ SRRRAEDRAV EPRRRPHRHR RAGARHRARR VRQGAPLCGR ARAVRQADRR ASGDPAEARR HGRADQRRAP ARASRGEAAH GRAAVPVGSV AGEAVRVRDG RARVFGCDPD SRRLRLPGRL RSRASLSRRA HHADLRRHQR SATDGDRAAA LSE
Validated NT Sequence
atcttactca cttaaagctg ccgcgcgatc accatccgtt gcacttcgct ggtgccttcg taaatctgcg tgatgcgcgc gtcgcgatag tgacgctcga cttcgtaatc gaccaggtag ccgtagccgc cgtgaatctg gatcgcatcc gaacacacgc gctcggccat ctcggacgcg aacagcttcg cctgcgacgc ttccgacagg cacggcagcc cggccgtgcg cagcttcgcc gcgtgatgca cgagcaggcg cgcggcgttg atctgcacgg ccatgtcggc gagcttctgc tggatcgcct gatgctcggc gatcggcttg ccgaactgca cgcgctcgcc cgcatagcgg cgcgccttgt cgaacgcggc gcgcgcgatg ccgagcgcct gcgcggcgat gccgatgcgg ccgccttcga ggttcgacag cgcgatcttc agcccttcgc cgcgattgcc gagcaggttg tcttccggaa tcgcgcagtt ctcgaacgtg atcgggcacg tatccgacgc gcggatgccc atcttcttct cgggcttgcc gacgatgaag cccggcgtgt cggtcggcac caggaacgcg gaaatgccgc gcttgccggc ttccgggtcg gtcatggcaa aaacgatcgc gacgccggcg cgctggccgt tggtcacgaa ctgcttcgcg ccgttcagca cccattggcc gtcgcgcagc tcggcccgcg tgcgcangtt gttcgcttcg gaaccggcat gcggctcggt caggcanaac gcgccgatca cgcgaccggn cgcnntgtcg gncanncngc ggtccntctg cgcgggcgtg ccgaancc
Expressed Protein Sequence
MAHHHHHHMD ELYTEDQRMI RDAARAFATE MLAPNAAQWD HDAHLPDAIV AQLGELGLLG MIVPQELGGS YTDYVAYALA MEEVAAGDAA CATMMSVHNS VGCGPILGFG TPAQKDRWLA DMAAGRVIGA FCLTEPHAGS EANNLRTRAE LRDGQWVLNG AKQFVTNGQR AGVAIVFAMT DPEAGKRGIS AFLVPTDTPG FIVGKPEKKM GIRASDTCPI TFENCAIPED NLLGNRGEGL KIALSNLEGG RIGIAAQALG IARAAFDKAR RYAGERVQFG KPIAEHQAIQ QKLADMAVQI NAARLLVHHA AKLRTAGLPC LSEASQAKLF ASEMAERVCS DAIQIHGGYG YLVDYEVERH YRDARITQIY EGTSEVQRMV IARQL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGACG AGCTTTACAC CGAAGACCAG CGGATGATCC GCGATGCGGC GCGCGCTTTT GCCACCGAGA TGCTGGCGCC GAACGCCGCG CAGTGGGACC ACGACGCGCA CCTGCCCGAC GCGATCGTCG CGCAGCTCGG CGAGCTCGGC CTGCTCGGGA TGATCGTGCC GCAGGAGCTG GGCGGCTCGT ATACGGACTA CGTGGCGTAT GCGCTGGCGA TGGAGGAAGT GGCGGCCGGC GACGCCGCGT GCGCGACGAT GATGAGCGTG CACAACTCGG TCGGCTGCGG GCCGATCCTC GGCTTCGGCA CGCCCGCGCA GAAGGACCGC TGGCTGGCCG ACATGGCGGC CGGTCGCGTG ATCGGCGCGT TCTGCCTGAC CGAGCCGCAT GCCGGTTCCG AAGCGAACAA CCTGCGCACG CGGGCCGAGC TGCGCGACGG CCAATGGGTG CTGAACGGCG CGAAGCAGTT CGTGACCAAC GGCCAGCGCG CCGGCGTCGC GATCGTTTTT GCCATGACCG ACCCGGAAGC CGGCAAGCGC GGCATTTCCG CGTTCCTGGT GCCGACCGAC ACGCCGGGCT TCATCGTCGG CAAGCCCGAG AAGAAGATGG GCATCCGCGC GTCGGATACG TGCCCGATCA CGTTCGAGAA CTGCGCGATT CCGGAAGACA ACCTGCTCGG CAATCGCGGC GAAGGGCTGA AGATCGCGCT GTCGAACCTC GAAGGCGGCC GCATCGGCAT CGCCGCGCAG GCGCTCGGCA TCGCGCGCGC CGCGTTCGAC AAGGCGCGCC GCTATGCGGG CGAGCGCGTG CAGTTCGGCA AGCCGATCGC CGAGCATCAG GCGATCCAGC AGAAGCTCGC CGACATGGCC GTGCAGATCA ACGCCGCGCG CCTGCTCGTG CATCACGCGG CGAAGCTGCG CACGGCCGGG CTGCCGTGCC TGTCGGAAGC GTCGCAGGCG AAGCTGTTCG CGTCCGAGAT GGCCGAGCGC GTGTGTTCGG ATGCGATCCA GATTCACGGC GGCTACGGCT ACCTGGTCGA TTACGAAGTC GAGCGTCACT ATCGCGACGC GCGCATCACG CAGATTTACG AAGGCACCAG CGAAGTGCAA CGGATGGTGA TCGCGCGGCA GCTTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT