BuceA.00027.b

Putative acyl-CoA dehydrogenase

CENTER ID: BuceA.00027.b
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00027.b.B1.GE36513 Full length( BuceA.00027.b ) 1 380
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.753
RefSeq: YP_002229876.1
UniProt: B4E9R8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFQHTEDRR MLADTLNRFI AEQYAFPVRD RIAQSAEGFD RAMWQRFAEL GTVGALFAEA DGGFGGAGFD IAVVFECLGR GLVVEPFLGA LLAGRALALA GGDAHRHRLA ALIDGSASAA FAHDEPGSHY ELTTVRTRAE RSGDGWVLTG AKGVVDQAAQ AAFFVVSARV SGNDDAAGIG LFVVPADAPG VSLRDYRKID GGRAAEVRFE RVALPADAAL GAPEALDGEA GAELLERVLG YGLLALSAEA LGAMDVAKEH TLDYLRTRKQ FGLPIGSFQA LQHRMADLLL EVEQARSAVI NAAAQLDAPR AVRERALAAA KYSIGRIGTL VAEESIQLHG GIGMTWELPL SHYAKRLVMI DHQLGDEDHH LARYIALSKQ
NT Sequence
ATGGATTTCC AGCACACAGA AGACCGCCGG ATGCTGGCGG ACACGTTGAA CCGCTTCATC GCCGAACAGT ACGCGTTCCC GGTGCGCGAC CGCATCGCGC AGTCGGCCGA AGGGTTCGAC CGCGCGATGT GGCAGCGCTT CGCCGAACTC GGCACGGTCG GAGCGCTGTT CGCCGAGGCC GATGGCGGCT TCGGCGGCGC GGGCTTCGAC ATCGCCGTGG TGTTCGAATG CCTCGGGCGC GGGCTCGTCG TCGAGCCGTT CCTCGGCGCG CTGCTCGCGG GCCGTGCGTT GGCGCTCGCC GGCGGCGATG CGCATCGCCA CCGGCTCGCC GCATTGATCG ACGGCAGCGC GAGCGCCGCG TTCGCGCACG ACGAACCGGG CTCGCACTAC GAACTGACGA CGGTGCGCAC GCGCGCCGAA CGGTCCGGCG ACGGCTGGGT GCTGACCGGC GCGAAAGGTG TCGTCGACCA GGCCGCGCAG GCGGCGTTCT TCGTCGTCAG CGCGCGCGTG TCCGGCAACG ACGACGCGGC CGGCATCGGC CTGTTCGTCG TGCCGGCCGA TGCGCCGGGC GTGTCGCTGC GCGACTACCG GAAGATCGAC GGCGGCCGTG CGGCCGAAGT GCGCTTCGAG CGTGTCGCGC TGCCGGCCGA CGCCGCGCTC GGCGCGCCGG AGGCGCTCGA CGGCGAAGCG GGCGCCGAGC TGCTCGAACG CGTGCTCGGC TACGGGCTGC TCGCGCTGTC GGCGGAAGCG CTCGGCGCGA TGGACGTCGC GAAGGAGCAC ACGCTCGACT ACCTGCGCAC ACGCAAGCAG TTCGGGTTGC CGATCGGCAG CTTCCAGGCG CTGCAGCACC GGATGGCCGA CCTGCTGCTC GAAGTCGAGC AGGCGCGCTC GGCCGTGATC AATGCGGCTG CGCAGCTCGA CGCGCCGCGC GCGGTGCGCG AGCGCGCGCT CGCGGCGGCG AAATACAGCA TCGGCCGGAT CGGCACGCTC GTCGCCGAGG AAAGCATCCA GCTGCACGGC GGGATCGGGA TGACGTGGGA GTTGCCGCTG TCGCACTACG CGAAGCGGCT GGTGATGATC GATCACCAGC TCGGTGACGA GGATCATCAT CTCGCGCGCT ACATCGCACT GTCGAAACAG
Details for BuceA.00027.b.B1.GE36513
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/7/2013
EXPECTED MW: 42kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 21
PERCENT COVERAGE: 94
Validated AA Sequence
MMILVTELVI DHHQPLRVVR QRQLPRHPDP AVQLDAFLGD ERADPADAVF RRRERALAHR ARRVELRSRI DHGRARLLDF EQQVGHPVLQ RLEAADRQPE LLACAQVVER VLLRDVHRAE RFRRQREQPV AEHAFEQLGA RFAVERLRRA ERGVGRQRDT LEAHFGRTAA VDLPVVAQRH ARRIGRHDEQ ADAGRVVVAG HARADDEXRR LRGLVDDTFR AGQHPAVAGX FGARAHRRQF XVRARFVVRE RGARAAVDXC GEPVAMRIAA GERQRTAREQ RAEERLDDEP APEAFEHHGD VEARAAEAAI GLGEQRSDRA EFGEALPHRA VEPFGRXRDA VAHRERVLFG DEAVQRVRQH PAVFCVLE
Validated NT Sequence
gcgagatgat gatcctcgtc accgagctgg tgatcgatca tcaccagccg cttcgcgtag tgcgacagcg gcaactccca cgtcatcccg atcccgccgt gcagctggat gctttcctcg gcgacgagcg tgccgatccg gccgatgctg tatttcgccg ccgcgagcgc gcgctcgcgc accgcgcgcg gcgcgtcgag ctgcgcagcc gcattgatca cggccgagcg cgcctgctcg acttcgagca gcaggtcggc catccggtgc tgcagcgcct ggaagctgcc gatcggcaac ccgaactgct tgcgtgtgcg caggtagtcg agcgtgtgct ccttcgcgac gtccatcgcg ccgagcgctt ccgccgacag cgcgagcagc ccgtagccga gcacgcgttc gagcagctcg gcgcccgctt cgccgtcgag cgcctccggc gcgccgagcg cggcgtcggc cggcagcgcg acacgctcga agcgcacttc ggccgcacgg ccgccgtcga tcttccggta gtcgcgcagc gacacgcccg gcgcatcggc cggcacgacg aacaggccga tgccggccgc gtcgtcgttg ccggacacgc gcgcgctgac gacgaanaac gccgcctgcg cggcctggtc gacgacacct ttcgcgccgg tcagcaccca gccgtcgccg gancgttcgg cgcgcgtgcg caccgtcgtc agttcntagt gcgagcccgg ttcgtcgtgc gcgaacgcgg cgctcgcgct gccgtcgatc nntgcggcga gccggtggcg atgcgcatcg ccgccggcga gcgccaacgc acggcccgcg agcagcgcgc cgaggaacgg ctcgacgacg agcccgcgcc cgaggcattc gaacaccacg gcgatgtnga agcccgcgcc gccgaagccg ccatcggcct cggcgaacag cgctccgacc gtgccgagtt cggcgaagcg ctgccacatc gcgcggtcga acccttcggc cgantgcgcg atgcggtcgc gcaccgggaa cgcgtactgt tcggcgatga agcggttcaa cgtgtccgcc agcatccggc ggtcttctgt gtgctggaaa t
Expected Protein Sequence
MAHHHHHHMD FQHTEDRRML ADTLNRFIAE QYAFPVRDRI AQSAEGFDRA MWQRFAELGT VGALFAEADG GFGGAGFDIA VVFECLGRGL VVEPFLGALL AGRALALAGG DAHRHRLAAL IDGSASAAFA HDEPGSHYEL TTVRTRAERS GDGWVLTGAK GVVDQAAQAA FFVVSARVSG NDDAAGIGLF VVPADAPGVS LRDYRKIDGG RAAEVRFERV ALPADAALGA PEALDGEAGA ELLERVLGYG LLALSAEALG AMDVAKEHTL DYLRTRKQFG LPIGSFQALQ HRMADLLLEV EQARSAVINA AAQLDAPRAV RERALAAAKY SIGRIGTLVA EESIQLHGGI GMTWELPLSH YAKRLVMIDH QLGDEDHHLA RYIALSKQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatt tccagcacac agaagaccgc cggatgctgg cggacacgtt gaaccgcttc atcgccgaac agtacgcgtt cccggtgcgc gaccgcatcg cgcagtcggc cgaagggttc gaccgcgcga tgtggcagcg cttcgccgaa ctcggcacgg tcggagcgct gttcgccgag gccgatggcg gcttcggcgg cgcgggcttc gacatcgccg tggtgttcga atgcctcggg cgcgggctcg tcgtcgagcc gttcctcggc gcgctgctcg cgggccgtgc gttggcgctc gccggcggcg atgcgcatcg ccaccggctc gccgcattga tcgacggcag cgcgagcgcc gcgttcgcgc acgacgaacc gggctcgcac tacgaactga cgacggtgcg cacgcgcgcc gaacggtccg gcgacggctg ggtgctgacc ggcgcgaaag gtgtcgtcga ccaggccgcg caggcggcgt tcttcgtcgt cagcgcgcgc gtgtccggca acgacgacgc ggccggcatc ggcctgttcg tcgtgccggc cgatgcgccg ggcgtgtcgc tgcgcgacta ccggaagatc gacggcggcc gtgcggccga agtgcgcttc gagcgtgtcg cgctgccggc cgacgccgcg ctcggcgcgc cggaggcgct cgacggcgaa gcgggcgccg agctgctcga acgcgtgctc ggctacgggc tgctcgcgct gtcggcggaa gcgctcggcg cgatggacgt cgcgaaggag cacacgctcg actacctgcg cacacgcaag cagttcgggt tgccgatcgg cagcttccag gcgctgcagc accggatggc cgacctgctg ctcgaagtcg agcaggcgcg ctcggccgtg atcaatgcgg ctgcgcagct cgacgcgccg cgcgcggtgc gcgagcgcgc gctcgcggcg gcgaaataca gcatcggccg gatcggcacg ctcgtcgccg aggaaagcat ccagctgcac ggcgggatcg ggatgacgtg ggagttgccg ctgtcgcact acgcgaagcg gctggtgatg atcgatcacc agctcggtga cgaggatcat catctcgcgc gctacatcgc actgtcgaaa cagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat