SSGCID: Target Page

BuceA.00025.a

NH(3)-dependent NAD(+) synthetase

CENTER ID:	BuceA.00025.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00025.a.B1.GE36717	Full length( BuceA.00025.a )		1	282

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.6285
RefSeq:	YP_002235079.1
UniProt:	B4EIP2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSADYASRQ RAIIAELNVA PHFDAEAEID RRIDFLAQYL RSTGLRTYVL GISGGVDSST AGRLAQLSVE RLRADGYDAR FIAMRLPNGV QNDEEDAQRA LAFVRADEVL TVDVKPAADA MLRSLVASGH AFDTPAQQDF VHGNIKARER MIAQYAVAGA RRGIVIGTDH AAESLMGFFT KFGDGGADIL PLAGLNKRRV RGVARALGGE ELIVMKVPTA DLEELRPLRP DEHAYGVTYD EIDDFLEGKP VADRVYETVL RFYDGSRHKR ALPYTMFDWP AA

NT Sequence

ATGACATCCG CCGATTACGC CAGCCGCCAA CGCGCGATCA TTGCCGAACT GAACGTCGCC CCGCACTTCG ACGCCGAAGC CGAAATCGAC CGCCGCATCG ATTTCCTCGC GCAGTACCTT CGTTCGACCG GCCTGCGGAC CTACGTGCTC GGCATCAGCG GCGGCGTCGA TTCGTCGACG GCCGGCCGGC TCGCGCAACT GTCGGTCGAG CGCCTGCGCG CCGACGGCTA CGATGCGCGC TTCATCGCGA TGCGCTTGCC GAACGGCGTG CAGAACGACG AGGAGGATGC ACAGCGCGCG CTGGCGTTCG TGCGCGCGGA CGAGGTATTG ACGGTCGACG TGAAGCCGGC CGCCGATGCG ATGCTCCGTT CGCTGGTCGC CTCCGGCCAC GCGTTCGACA CCCCGGCGCA ACAGGATTTC GTGCACGGCA ACATCAAGGC GCGCGAGCGC ATGATCGCGC AGTACGCGGT GGCCGGCGCG CGGCGCGGCA TCGTGATCGG CACCGATCAC GCGGCCGAAT CGCTGATGGG GTTCTTCACG AAGTTCGGCG ACGGCGGCGC GGACATCCTG CCGCTCGCGG GCCTGAACAA GCGCCGCGTG CGCGGGGTGG CGCGCGCGCT CGGCGGCGAG GAGCTGATCG TGATGAAGGT GCCGACGGCC GACCTCGAGG AACTGCGCCC GCTGCGCCCG GACGAGCACG CGTATGGCGT GACCTACGAC GAGATCGACG ATTTCCTCGA AGGCAAGCCG GTCGCCGACC GCGTCTACGA AACGGTGCTG CGCTTCTACG ACGGCTCGCG CCACAAGCGC GCGCTGCCGT ACACGATGTT CGACTGGCCG GCCGCG

Details for BuceA.00025.a.B1.GE36717

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMT SADYASRQRA IIAELNVAPH FDAEAEIDRR IDFLAQYLRS TGLRTYVLGI SGGVDSSTAG RLAQLSVERL RADGYDARFI AMRLPNGVQN DEEDAQRALA FVRADEVLTV DVKPAADAML RSLVASGHAF DTPAQQDFVH GNIKARERMI AQYAVAGARR GIVIGTDHAA ESLMGFFTKF GDGGADILPL AGLNKRRVRG VARALGGEEL IVMKVPTADL EELRPLRPDE HAYGVXYDEI DDFLEGKPVA DRVYXTVLRF YDGSRHKRAL PYTMFDWPAA

Validated NT Sequence

ctnnnnnnnt tnnttacttt aagaaggaga nataccatgg ctcaccacca ccaccaccat atgacatccg ccgattacgc cagccgccaa cgcgcgatca ttgccgaact gaacgtcgcc ccgcacttcg acgccgaagc cgaaatcgac cgccgcatcg atttcctcgc gcagtacctt cgttcgaccg gcctgcggac ctacgtgctc ggcatcagcg gcggcgtcga ttcgtcgacg gccggccggc tcgcgcaact gtcggtcgag cgcctgcgcg ccgacggcta cgatgcgcgc ttcatcgcga tgcgcttgcc gaacggcgtg cagaacgacg aggaggatgc acagcgcgcg ctggcgttcg tgcgcgcgga cgaggtattg acggtcgacg tgaagccggc cgccgatgcg atgctccgtt cgctggtcgc ctccggccac gcgttcgaca ccccggcgca acaggatttc gtgcacggca acatcaaggc gcgcgagcgc atgatcgcgc agtacgcggt ggccggcgcg cggcgcggca tcgtgatcgg caccgatcac gcggccgaat cgctgatggg gttcttcacg aagttcggcg acggcggcgc ggacatcctg ccgctcgcgg gcctgaacaa gcgccgcgtg cgcggggtgg cgcgcgcgct cggcggcgag gagctgatcg tgatgaaggt gccgacggcc gacctcgagg aactgcgccc gctgcgcccg gacgagcacg cgtatggcgt ganctacgac gagatcgacg atttcctcga aggcaagccg gtcgccgacc gcgtctacna aacggtgctg cgcttctacg acggctcgcg ccacaagcgc gcgctgccgt acacgatgtt cgactggccg gccgcgtaag tgagtaanan agnnncnnnc c

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacat ccgccgatta cgccagccgc caacgcgcga tcattgccga actgaacgtc gccccgcact tcgacgccga agccgaaatc gaccgccgca tcgatttcct cgcgcagtac cttcgttcga ccggcctgcg gacctacgtg ctcggcatca gcggcggcgt cgattcgtcg acggccggcc ggctcgcgca actgtcggtc gagcgcctgc gcgccgacgg ctacgatgcg cgcttcatcg cgatgcgctt gccgaacggc gtgcagaacg acgaggagga tgcacagcgc gcgctggcgt tcgtgcgcgc ggacgaggta ttgacggtcg acgtgaagcc ggccgccgat gcgatgctcc gttcgctggt cgcctccggc cacgcgttcg acaccccggc gcaacaggat ttcgtgcacg gcaacatcaa ggcgcgcgag cgcatgatcg cgcagtacgc ggtggccggc gcgcggcgcg gcatcgtgat cggcaccgat cacgcggccg aatcgctgat ggggttcttc acgaagttcg gcgacggcgg cgcggacatc ctgccgctcg cgggcctgaa caagcgccgc gtgcgcgggg tggcgcgcgc gctcggcggc gaggagctga tcgtgatgaa ggtgccgacg gccgacctcg aggaactgcg cccgctgcgc ccggacgagc acgcgtatgg cgtgacctac gacgagatcg acgatttcct cgaaggcaag ccggtcgccg accgcgtcta cgaaacggtg ctgcgcttct acgacggctc gcgccacaag cgcgcgctgc cgtacacgat gttcgactgg ccggccgcgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat