SSGCID: Target Page

BuceA.00024.c

Putative taurine dioxygenase

CENTER ID:	BuceA.00024.c
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00024.c.B1.GE36510	Full length( BuceA.00024.c )		1	317

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.4837
RefSeq:	YP_002233739.1
UniProt:	B4EGA2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSEVQHAAPS FSPASHAAPR PAAVPLQLRQ VAGRIGAEIA DVRLSATLDD ATFDAIQAAL LRHKVLFFRG QHHLDDTAQE AFARRFGDTV AHPTVPSVDG SAALLELDSA HGARANSWHT DVTFVDAYPK ISILRAVVIP PFGGDTVWAN TAAAYAHLPD PLRALADTLW ALHTNAYDYA STHVHADDAQ LKRYREVFTS TVYETEHPVV RVHPETGERT LVLGHFVQRL KGLSAQDSAH LLQVFHEHVT RLENTVRWNW REGDVAIWDN RATQHYAIND YGDARRVVRR ATVHGDVPVG IDGRRSVLLK GPGATLQ

NT Sequence

ATGTCCGAAG TCCAGCACGC CGCTCCGTCG TTTTCGCCCG CTTCGCATGC CGCGCCGCGC CCGGCCGCCG TCCCGCTCCA GCTTCGCCAG GTCGCCGGCC GGATCGGCGC CGAAATCGCC GACGTGCGGC TGTCGGCCAC GCTCGACGAT GCGACGTTCG ACGCGATCCA GGCCGCGCTG CTGCGCCACA AGGTGCTGTT CTTCCGTGGC CAGCACCATC TCGACGACAC CGCGCAGGAA GCATTCGCCC GCCGCTTCGG CGACACGGTC GCGCACCCGA CCGTGCCGTC CGTCGACGGC AGCGCGGCCC TGCTCGAACT CGATTCCGCG CACGGCGCAC GCGCCAATTC GTGGCACACC GACGTGACCT TCGTCGACGC GTACCCGAAG ATCTCGATCC TGCGCGCGGT CGTGATTCCG CCATTCGGCG GCGATACCGT CTGGGCCAAC ACGGCCGCCG CGTACGCGCA TCTGCCCGAC CCGCTGCGTG CGCTCGCCGA CACGCTGTGG GCGCTGCATA CGAACGCCTA CGACTATGCG TCGACACACG TGCATGCCGA CGATGCGCAA CTGAAGCGCT ACCGCGAAGT GTTCACGTCG ACCGTCTACG AAACCGAGCA CCCGGTCGTG CGCGTGCATC CGGAAACCGG CGAGCGCACG CTCGTGCTCG GCCACTTCGT GCAGCGACTC AAGGGGCTGT CGGCGCAGGA TTCCGCGCAT CTGCTGCAGG TGTTCCACGA GCACGTGACG CGCCTCGAAA ACACCGTGCG CTGGAACTGG CGGGAAGGCG ACGTCGCGAT CTGGGACAAC CGCGCAACCC AGCACTACGC GATCAACGAC TACGGTGATG CGCGCCGCGT CGTGCGCCGC GCGACCGTTC ACGGCGACGT GCCGGTCGGC ATCGACGGCC GCCGGAGCGT CCTGCTGAAG GGGCCGGGTG CCACGCTGCA A

Details for BuceA.00024.c.B1.GE36510

HARVESTED ON:	1/9/2013
SEQUENCED ON:	1/7/2013
EXPECTED MW:	36kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

tcttactcac ttattgcagc gtggcacccg gccccttcag caggacgctc cggcggccgt cgatgccgac cggcacgtcg ccgtgaacgg tcgcgcggcg cacgacgcgg cgcgcatcac cgtagtcgtt gatcgcgtag tgctgggttg cgcggttgtc ccagatcgcg acgtcgcctt cccgccagtt ccagcgcacg gtgttttcga ggcgcgtcac gtgctcgtgg aacacctgca gcagatgcgc ggaatcctgc gccgacagcc ccttgagtcg ctgcacgaag tggccgagca cgagcgtgcg ctcgccggtt tccggatgca cgcgcacgac cgggtgctcg gtttcgtaga cggtcgacgt gaacacttcg cggtagcgct tcagttgcgc atcgtcggca tgcacgtgtg tcgacgcata gtcgtaggcg ttcgtatgca gcgcccacag cgtgtcggcg agcgcacgca gcgggtcggg cagatgcgcg tacgcggcgg ccgtgttggc ccagacggta tcgccgccga atggcggaat cacgaccgcg cgcaggatcg agatcttcgg gtacgcgtcg acgaaggtca cgtcggtgtg ccacgaattg gcgcgtgcgc cgtgcgcgga atcgagttcg agcagggccg cgctgccgtc gacggacggc acggtcgggt gcgcgaccgt gtcgccgaag cggcgggcga atgcttcctg cgcggtgtcg tcgagatggt gctggccacg gaagaacagc accttgtggc gcagcagcgc ggcctggatc gcgtcgaacg tcgcatcgtc gagcgtggcc gacagccgca cgtcggcgat ttcggcgccg atccggccgg cgacctggcg aagctggagc gggacggcgg ccgggcgcgg cgcggcatgc gaagcgggcg aaaacgacgg agcggcgtgc tggacttcgg acatatggtg gtg

Expected Protein Sequence

MAHHHHHHMS EVQHAAPSFS PASHAAPRPA AVPLQLRQVA GRIGAEIADV RLSATLDDAT FDAIQAALLR HKVLFFRGQH HLDDTAQEAF ARRFGDTVAH PTVPSVDGSA ALLELDSAHG ARANSWHTDV TFVDAYPKIS ILRAVVIPPF GGDTVWANTA AAYAHLPDPL RALADTLWAL HTNAYDYAST HVHADDAQLK RYREVFTSTV YETEHPVVRV HPETGERTLV LGHFVQRLKG LSAQDSAHLL QVFHEHVTRL ENTVRWNWRE GDVAIWDNRA TQHYAINDYG DARRVVRRAT VHGDVPVGID GRRSVLLKGP GATLQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg aagtccagca cgccgctccg tcgttttcgc ccgcttcgca tgccgcgccg cgcccggccg ccgtcccgct ccagcttcgc caggtcgccg gccggatcgg cgccgaaatc gccgacgtgc ggctgtcggc cacgctcgac gatgcgacgt tcgacgcgat ccaggccgcg ctgctgcgcc acaaggtgct gttcttccgt ggccagcacc atctcgacga caccgcgcag gaagcattcg cccgccgctt cggcgacacg gtcgcgcacc cgaccgtgcc gtccgtcgac ggcagcgcgg ccctgctcga actcgattcc gcgcacggcg cacgcgccaa ttcgtggcac accgacgtga ccttcgtcga cgcgtacccg aagatctcga tcctgcgcgc ggtcgtgatt ccgccattcg gcggcgatac cgtctgggcc aacacggccg ccgcgtacgc gcatctgccc gacccgctgc gtgcgctcgc cgacacgctg tgggcgctgc atacgaacgc ctacgactat gcgtcgacac acgtgcatgc cgacgatgcg caactgaagc gctaccgcga agtgttcacg tcgaccgtct acgaaaccga gcacccggtc gtgcgcgtgc atccggaaac cggcgagcgc acgctcgtgc tcggccactt cgtgcagcga ctcaaggggc tgtcggcgca ggattccgcg catctgctgc aggtgttcca cgagcacgtg acgcgcctcg aaaacaccgt gcgctggaac tggcgggaag gcgacgtcgc gatctgggac aaccgcgcaa cccagcacta cgcgatcaac gactacggtg atgcgcgccg cgtcgtgcgc cgcgcgaccg ttcacggcga cgtgccggtc ggcatcgacg gccgccggag cgtcctgctg aaggggccgg gtgccacgct gcaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat