BuceA.00020.z

Putative aldehyde dehydrogenase

CENTER ID: BuceA.00020.z
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00020.z.B1.GE36507 Full length( BuceA.00020.z ) 1 542
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.4181
RefSeq: YP_002233122.1
UniProt: B4EK01

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPDSASLQHL FPALQDIPAE FRLASPIHQR VSLVDGELRP WDGATKTVLS PVCVRQPDGG VEQVEIGSYP VMGETESDAA LDAAVRAYDA GRGEWPTMKV EQRIACMQDF IKRMVAQRER VVNLIMWEIG KSLADSQKEF DRTVTYMTQT IDALKELDNA NSRFVIAEGT IGQIRRTPLG VVLCMGPYNY PLNETFATLI PALLMGNTVV FKPPQYGTLL FEPLLEAFRD AFPKGVINTI YAPGAVVVPH MLASGKINVL ALIGSSKVAD HLKKQHPKSH RLRAILGLDA KNAAIVLPDA DLDLTVKECL LGALSFNGQR CTALKMLLVH RSIVDEFLKR FTAALEQLKI GMPWEKGVSI TPLPGMHRTA YMTDAIDDAR AKGAQVVNHQ GGEFSKTLFY PAVVYPVSEG MKLYREEQFG PIIPVAPFDD VETALDYVTT SEHGQQVSIF GSDPAQIGAL VDPLVNQVCR VNINCQCQRG PDVFPFAGRK DSAEGTLSVS DALRAFSIRS MVAAKQTDGS KQLLDSIVSD HHSKFINTGF IF
NT Sequence
ATGCCCGATT CCGCCTCCCT GCAGCACCTC TTTCCCGCCT TACAGGACAT CCCCGCCGAA TTCCGGCTGG CTTCGCCGAT TCACCAGCGC GTGTCGCTCG TCGACGGCGA ATTGCGACCG TGGGACGGCG CGACCAAGAC CGTGCTGTCG CCCGTGTGCG TGCGGCAGCC GGACGGCGGC GTCGAGCAGG TCGAGATCGG CAGCTATCCG GTGATGGGCG AAACGGAAAG CGACGCGGCG CTCGACGCCG CGGTACGCGC GTACGACGCC GGCCGCGGCG AGTGGCCGAC GATGAAGGTC GAGCAGCGCA TCGCGTGCAT GCAGGATTTC ATCAAGCGGA TGGTCGCGCA GCGCGAACGC GTCGTGAACC TGATCATGTG GGAGATCGGC AAGAGCCTCG CCGATTCGCA GAAGGAATTC GACCGCACCG TCACGTACAT GACGCAGACG ATCGACGCGC TGAAGGAGCT GGACAACGCG AACTCGCGCT TCGTGATCGC GGAGGGCACG ATCGGCCAGA TCCGCCGCAC GCCGCTCGGC GTCGTGCTGT GCATGGGCCC GTACAACTAT CCGCTGAACG AAACCTTCGC GACGCTGATC CCCGCGCTGC TGATGGGCAA CACCGTGGTG TTCAAGCCGC CGCAGTACGG CACGCTGCTG TTCGAGCCGC TGCTGGAAGC GTTCCGCGAC GCGTTCCCGA AGGGCGTGAT CAACACGATC TACGCGCCGG GCGCGGTGGT CGTGCCGCAC ATGCTCGCAT CGGGCAAGAT CAACGTGCTC GCGCTGATCG GCTCGAGCAA GGTCGCCGAT CACCTGAAGA AGCAGCACCC GAAGTCGCAC CGGCTGCGCG CGATCCTCGG TCTCGATGCG AAGAACGCGG CGATCGTGCT GCCCGACGCC GATCTCGACC TCACCGTGAA GGAGTGCCTG CTCGGCGCGC TGTCGTTCAA CGGCCAGCGC TGTACCGCGC TGAAGATGCT GCTCGTGCAT CGCTCGATCG TCGACGAATT CCTGAAGCGC TTCACCGCCG CGCTCGAACA GCTGAAGATC GGCATGCCGT GGGAAAAAGG CGTCAGCATC ACCCCGCTGC CCGGCATGCA TCGCACCGCG TACATGACGG ACGCGATCGA CGACGCGAGG GCGAAGGGCG CGCAGGTCGT CAACCATCAG GGCGGCGAAT TCAGCAAGAC GCTGTTCTAC CCGGCCGTCG TGTATCCGGT GTCCGAAGGA ATGAAGCTGT ACCGCGAGGA GCAGTTCGGG CCGATCATTC CGGTCGCGCC GTTCGACGAC GTGGAAACCG CGCTCGACTA CGTGACGACG TCGGAGCACG GCCAGCAGGT CAGCATCTTC GGTTCCGATC CGGCGCAGAT CGGCGCGCTC GTCGATCCGC TCGTGAACCA GGTGTGCCGC GTGAACATCA ACTGTCAGTG CCAGCGCGGG CCCGACGTGT TCCCGTTCGC CGGACGCAAG GATTCGGCCG AGGGCACGCT GTCGGTGAGC GACGCGCTGC GCGCGTTCTC GATCCGCTCG ATGGTCGCGG CGAAGCAGAC CGACGGCAGC AAGCAGCTGC TCGACTCGAT CGTGTCGGAT CACCACTCGA AGTTCATCAA TACGGGCTTC ATTTTC
Details for BuceA.00020.z.B1.GE36507
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/7/2013
EXPECTED MW: 60kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 84
Validated AA Sequence
MGETESDAAL DAAVRAYDAG RGEWPTMKVE QRIACMQDFI KRMVAQRERV VNLIMWEIGK SLADSQKEFD RTVTYMTQTI DALKELDNAN SRFVIAEGTI GQIRRTPLGV VLCMGPYNYP LNETFATLIP ALLMGNTVVF KPPQYGTLLF EPLLEAFRDA FPKGVINTIY APGAVVVPHM LASGKINVLA LIGSSKVADH LKKQHPKSHR LRAILGLDAK NAAIVLPDAD LDXTVKECLL GALSFNGQRC TALKMLLVHR SIVDEFXKRF TAALEQLKIG MPWEKGVSIT PLPGMHRTAY MTDAIDDARA KGAQVVNHQG GEFSKTLFYP AVVYPVSEGM KLYREEQFGP IIPVAPFDDV ETALDYVTTS EHGQQVSIFG SDPAQIGALV DPLVNQVCRV NINCQCQRGP DVFPFAGRKD SAEGTLSVSD ALRAFSIRSM VAAKQTDGSK QLLDSIVSDH HS
Validated NT Sequence
cttcgagtgg tgatccgaca cgatcgagtc gagcagctgc ttgctgccgt cggtctgctt cgccgcgacc atcgagcgga tcgagaacgc gcgcagcgcg tcgctcaccg acagcgtgcc ctcggccgaa tccttgcgtc cggcgaacgg gaacacgtcg ggcccgcgct ggcactgaca gttgatgttc acgcggcaca cctggttcac gagcggatcg acgagcgcgc cgatctgcgc cggatcggaa ccgaagatgc tgacctgctg gccgtgctcc gacgtcgtca cgtagtcgag cgcggtttcc acgtcgtcga acggcgcgac cggaatgatc ggcccgaact gctcctcgcg gtacagcttc attccttcgg acaccggata cacgacggcc gggtagaaca gcgtcttgct gaattcgccg ccctgatggt tgacgacctg cgcgcccttc gccctcgcgt cgtcgatcgc gtccgtcatg tacgcggtgc gatgcatgcc gggcagcggg gtgatgctga cgcctttttc ccacggcatg ccgatcttca gctgttcgag cgcggcggtg aagcgcttca ngaattcgtc gacgatcgag cgatgcacga gcagcatctt cagcgcggta cagcgctggc cgttgaacga cagcgcgccg agcaggcact ccttcacggt gangtcgaga tcggcgtcgg gcagcacgat cgccgcgttc ttcgcatcga gaccgaggat cgcgcgcagc cggtgcgact tcgggtgctg cttcttcagg tgatcggcga ccttgctcga gccgatcagc gcgagcacgt tgatcttgcc cgatgcgagc atgtgcggca cgaccaccgc gcccggcgcg tagatcgtgt tgatcacgcc cttcgggaac gcgtcgcgga acgcttccag cagcggctcg aacagcagcg tgccgtactg cggcggcttg aacaccacgg tgttgcccat cagcagcgcg gggatcagcg tcgcgaaggt ttcgttcagc ggatagttgt acgggcccat gcacagcacg acgccgagcg gcgtgcggcg gatctggccg atcgtgccct ccgcgatcac gaagcgcgag ttcgcgttgt ccagctcctt cagcgcgtcg atcgtctgcg tcatgtacgt gacggtgcgg tcgaattcct tctgcgaatc ggcgaggctc ttgccgatct cccacatgat caggttcacg acgcgttcgc gctgcgcgac catccgcttg atgaaatcct gcatgcacgc gatgcgctgc tcgaccttca tcgtcggcca ctcgccgcgg ccggcgtcgt acgcgcgtac cgcggcgtcg agcgccgcgt cgctttccgt ttcgcccatc accggatagc tgccgatctc gacctgctcg acgccgccgt ccggctgccg cacgcacacg ggcgacagca cggtcttggt cgcgccgtcc cacggtcgca attcgccgtc gacgagcgac acgcgctggt gaatcggcga agccagccgg aattcggcgg ggatgtcctg taaggcggga aagaggtgct gcagggaggc ggaatcgg
Expected Protein Sequence
MAHHHHHHMP DSASLQHLFP ALQDIPAEFR LASPIHQRVS LVDGELRPWD GATKTVLSPV CVRQPDGGVE QVEIGSYPVM GETESDAALD AAVRAYDAGR GEWPTMKVEQ RIACMQDFIK RMVAQRERVV NLIMWEIGKS LADSQKEFDR TVTYMTQTID ALKELDNANS RFVIAEGTIG QIRRTPLGVV LCMGPYNYPL NETFATLIPA LLMGNTVVFK PPQYGTLLFE PLLEAFRDAF PKGVINTIYA PGAVVVPHML ASGKINVLAL IGSSKVADHL KKQHPKSHRL RAILGLDAKN AAIVLPDADL DLTVKECLLG ALSFNGQRCT ALKMLLVHRS IVDEFLKRFT AALEQLKIGM PWEKGVSITP LPGMHRTAYM TDAIDDARAK GAQVVNHQGG EFSKTLFYPA VVYPVSEGMK LYREEQFGPI IPVAPFDDVE TALDYVTTSE HGQQVSIFGS DPAQIGALVD PLVNQVCRVN INCQCQRGPD VFPFAGRKDS AEGTLSVSDA LRAFSIRSMV AAKQTDGSKQ LLDSIVSDHH SKFINTGFIF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg attccgcctc cctgcagcac ctctttcccg ccttacagga catccccgcc gaattccggc tggcttcgcc gattcaccag cgcgtgtcgc tcgtcgacgg cgaattgcga ccgtgggacg gcgcgaccaa gaccgtgctg tcgcccgtgt gcgtgcggca gccggacggc ggcgtcgagc aggtcgagat cggcagctat ccggtgatgg gcgaaacgga aagcgacgcg gcgctcgacg ccgcggtacg cgcgtacgac gccggccgcg gcgagtggcc gacgatgaag gtcgagcagc gcatcgcgtg catgcaggat ttcatcaagc ggatggtcgc gcagcgcgaa cgcgtcgtga acctgatcat gtgggagatc ggcaagagcc tcgccgattc gcagaaggaa ttcgaccgca ccgtcacgta catgacgcag acgatcgacg cgctgaagga gctggacaac gcgaactcgc gcttcgtgat cgcggagggc acgatcggcc agatccgccg cacgccgctc ggcgtcgtgc tgtgcatggg cccgtacaac tatccgctga acgaaacctt cgcgacgctg atccccgcgc tgctgatggg caacaccgtg gtgttcaagc cgccgcagta cggcacgctg ctgttcgagc cgctgctgga agcgttccgc gacgcgttcc cgaagggcgt gatcaacacg atctacgcgc cgggcgcggt ggtcgtgccg cacatgctcg catcgggcaa gatcaacgtg ctcgcgctga tcggctcgag caaggtcgcc gatcacctga agaagcagca cccgaagtcg caccggctgc gcgcgatcct cggtctcgat gcgaagaacg cggcgatcgt gctgcccgac gccgatctcg acctcaccgt gaaggagtgc ctgctcggcg cgctgtcgtt caacggccag cgctgtaccg cgctgaagat gctgctcgtg catcgctcga tcgtcgacga attcctgaag cgcttcaccg ccgcgctcga acagctgaag atcggcatgc cgtgggaaaa aggcgtcagc atcaccccgc tgcccggcat gcatcgcacc gcgtacatga cggacgcgat cgacgacgcg agggcgaagg gcgcgcaggt cgtcaaccat cagggcggcg aattcagcaa gacgctgttc tacccggccg tcgtgtatcc ggtgtccgaa ggaatgaagc tgtaccgcga ggagcagttc gggccgatca ttccggtcgc gccgttcgac gacgtggaaa ccgcgctcga ctacgtgacg acgtcggagc acggccagca ggtcagcatc ttcggttccg atccggcgca gatcggcgcg ctcgtcgatc cgctcgtgaa ccaggtgtgc cgcgtgaaca tcaactgtca gtgccagcgc gggcccgacg tgttcccgtt cgccggacgc aaggattcgg ccgagggcac gctgtcggtg agcgacgcgc tgcgcgcgtt ctcgatccgc tcgatggtcg cggcgaagca gaccgacggc agcaagcagc tgctcgactc gatcgtgtcg gatcaccact cgaagttcat caatacgggc ttcattttct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat