BuceA.00020.y
Putative aldehyde dehydrogenase
| CENTER ID: | BuceA.00020.y |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00020.y.B1.GE36506 | Full length( BuceA.00020.y ) | 1 | 488 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00020.y.B1.PS01792 | Full length( BuceA.00020.y ) | 1 | 488 |
Structures
4O6R
DEPOSITED: 12/23/2013
DETERMINATION: XRay
CLONE: BuceA.00020.y.B1.GE36506
PROTEIN: BuceA.00020.y.B1.PS01792
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126638 |
| PATRIC ID: | fig|216591.5.peg.4151 |
| RefSeq: | YP_002233092.1 |
| UniProt: | B4EJX1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQTQLFIDGR FVDAVDRGTI DVLNPHDGSV ITKIAAATAA DVDLAVDAAT RAFPAWSAMP AAERGRLLLR LADAIEANTE ALAQLESLDT GHPIRDSRAL DVPRTAACFR YFGGMADKLQ GSVIPVDTGF LNYVQRAPIG VVGQIVPWNF PLMFTSWKMG PALAAGNTVV LKPSEITPLS TLRIVELMAE VGFPAGVVNI VPGYGHTAGQ RLAEHPGVGK IAFTGSTATG RRIVEASQGN LKRVQLELGG KGANIVFDDA NLDAAINGAA WAIFHNQGQA CIAGSRLVLH ERIADAFLER FVALASSIRI GNPLDPNTEM GPLTSKQHLD RVLSYVDVAR EQGGRVLTGG SAPQDPALAN GYYVRPTIVE AKHATDRVAQ EEVFGPFVTV LRFGSDDEAL AIANATEYGL GSGLWTNDLS RAHRMAARID AGMCWINCYK RVNPGSPFGG VGKSGYGREM GFEAMHDYTE ARSVWVNVDG NVPPHFKR
NT Sequence
ATGCAAACCC AACTCTTCAT CGACGGGCGC TTCGTTGACG CCGTCGATCG CGGCACGATC GACGTGCTGA ACCCGCACGA TGGCTCCGTC ATCACGAAGA TCGCGGCTGC CACCGCGGCG GACGTCGATC TGGCCGTCGA CGCCGCGACG CGCGCCTTCC CGGCCTGGTC GGCGATGCCG GCCGCCGAGC GCGGCCGCCT GCTGCTGCGC CTGGCCGATG CGATCGAAGC GAACACGGAA GCGCTGGCGC AGCTCGAATC GCTGGACACC GGCCACCCGA TTCGCGATTC GCGGGCGCTC GACGTGCCGC GCACGGCGGC CTGCTTCCGC TATTTCGGCG GCATGGCCGA CAAGCTGCAA GGCTCGGTGA TACCGGTCGA CACCGGCTTC CTGAACTACG TGCAGCGCGC GCCGATCGGC GTGGTCGGCC AGATCGTGCC GTGGAATTTT CCGCTGATGT TCACGAGCTG GAAGATGGGC CCGGCGCTGG CGGCCGGCAA CACGGTGGTG CTCAAGCCGT CGGAAATCAC GCCGCTGTCG ACGTTGCGCA TCGTCGAGCT GATGGCCGAG GTCGGATTTC CTGCCGGCGT CGTCAACATC GTGCCCGGCT ACGGCCATAC GGCCGGCCAG CGGCTCGCCG AACATCCGGG CGTCGGCAAG ATCGCGTTCA CCGGCTCGAC GGCGACCGGC CGGCGAATCG TCGAAGCATC GCAGGGCAAC CTGAAGCGCG TGCAACTGGA ACTGGGCGGC AAGGGCGCCA ACATCGTGTT CGACGATGCC AACCTCGACG CGGCCATCAA CGGCGCCGCG TGGGCGATCT TCCACAACCA GGGGCAGGCG TGTATCGCGG GGTCGCGTCT GGTGCTGCAC GAACGCATCG CGGACGCGTT TCTCGAACGC TTCGTCGCGC TCGCGTCGTC GATCCGGATC GGCAATCCGC TCGACCCGAA TACCGAAATG GGGCCGCTCA CGTCGAAGCA GCACCTCGAT CGCGTGCTGT CGTATGTCGA CGTCGCGCGC GAGCAGGGCG GCCGCGTGCT GACGGGCGGC AGCGCGCCGC AGGACCCGGC GCTCGCCAAT GGCTATTACG TGCGCCCGAC GATCGTCGAG GCGAAGCATG CGACCGACCG CGTCGCGCAG GAAGAAGTGT TCGGCCCGTT CGTCACCGTG CTGCGCTTCG GCAGCGACGA CGAAGCGCTG GCCATCGCCA ACGCGACGGA ATACGGGCTC GGCAGCGGCC TGTGGACCAA CGACCTGTCG CGCGCCCACC GGATGGCGGC GCGGATCGAC GCGGGCATGT GCTGGATCAA CTGCTACAAG CGCGTGAATC CGGGCAGCCC GTTCGGCGGC GTCGGCAAGT CGGGCTACGG CCGCGAGATG GGCTTCGAGG CGATGCACGA CTACACGGAA GCGCGCTCGG TGTGGGTCAA CGTCGACGGC AACGTGCCGC CGCATTTCAA GCGC
Details for BuceA.00020.y.B1.GE36506
| HARVESTED ON: | 1/9/2013 |
| SEQUENCED ON: | 1/7/2013 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 51kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 84 |
Validated AA Sequence
MPAAERGRLL LRLADAIEAN TEALAQLESL DTGHPIRDSR ALDVPRTAAC FRYFGGMADK LQGSVIPVDT GFLNYVQRAP IGVVGQIVPW NFPLMFTSWK MGPALAAGNT VVLKPSEITP LSTLRIVELM AEVGFPAGVV NIVPGYGHTA GQRLAEHPGV GKIAFTGSTA TGRRIVEASQ GNLKRVQLEL GGKGANIVFD DANLDAAING AAWAIFHNQG QACIAGSRLV LHERIADAFL ERFVALASSI RIGNPLDPNT EMGPLTXKQH LDRVLXYVDV AREQGGRVLT GGSAPQDPAL ANGYYVRPTI VEAKHATDRV AQEEVFGPFV TVLRFGSDDE ALAIANATEY GLGSGLWTND LSRAHRMAAR IDAGMCWINC YKRVNPGSPF GGVGKSGYGR EMGFEAMHDY TEARSVWVNV D
Validated NT Sequence
gccgtcgacg ttgacccaca ccgagcgcgc ttccgtgtag tcgtgcatcg cctcgaagcc catctcgcgg ccgtagcccg acttgccgac gccgccgaac gggctgcccg gattcacgcg cttgtagcag ttgatccagc acatgcccgc gtcgatccgc gccgccatcc ggtgggcgcg cgacaggtcg ttggtccaca ggccgctgcc gagcccgtat tccgtcgcgt tggcgatggc cagcgcttcg tcgtcgctgc cgaagcgcag cacggtgacg aacgggccga acacttcttc ctgcgcgacg cggtcggtcg catgcttcgc ctcgacgatc gtcgggcgca cgtaatancc attggcgagc gccgggtcct gcggcgcgct gccgcccgtc agcacgcggc cgccctgctc gcgcgcgacg tcgacatacn acagcacgcg atcgaggtgc tgcttcnacg tgagcggccc catttcggta ttcgggtcga gcggattgcc gatccggatc gacgacgcga gcgcgacgaa gcgttcgaga aacgcgtccg cgatgcgttc gtgcagcacc agacgcgacc ccgcgataca cgcctgcccc tggttgtgga agatcgccca cgcggcgccg ttgatggccg cgtcgaggtt ggcatcgtcg aacacgatgt tggcgccctt gccgcccagt tccagttgca cgcgcttcag gttgccctgc gatgcttcga cgattcgccg gccggtcgcc gtcgagccgg tgaacgcgat cttgccgacg cccggatgtt cggcgagccg ctggccggcc gtatggccgt agccgggcac gatgttgacg acgccggcag gaaatccgac ctcggccatc agctcgacga tgcgcaacgt cgacagcggc gtgatttccg acggcttgag caccaccgtg ttgccggccg ccagcgccgg gcccatcttc cagctcgtga acatcagcgg aaaattccac ggcacgatct ggccgaccac gccgatcggc gcgcgctgca cgtagttcag gaagccggtg tcgaccggta tcaccgagcc ttgcagcttg tcggccatgc cgccgaaata gcggaagcag gccgccgtgc gcggcacgtc gagcgcccgc gaatcgcgaa tcgggtggcc ggtgtccagc gattcgagct gcgccagcgc ttccgtgttc gcttcgatcg catcggccag gcgcagcagc aggcggccgc gctcggcggc cggcatcgcc gaccaggccg ggaaggcgcg cgtcgcggcg tcgacggcca gatcgacgtc cgccgcggtg gcagccgcga tcttcgtgat gacggagcca tcgtgcgggt tcagcacgtc gatcgtgccg cgatcgacgg cgtcaacgaa gcgcccgtcg atgaagagtt gggttt
Expected Protein Sequence
MAHHHHHHMQ TQLFIDGRFV DAVDRGTIDV LNPHDGSVIT KIAAATAADV DLAVDAATRA FPAWSAMPAA ERGRLLLRLA DAIEANTEAL AQLESLDTGH PIRDSRALDV PRTAACFRYF GGMADKLQGS VIPVDTGFLN YVQRAPIGVV GQIVPWNFPL MFTSWKMGPA LAAGNTVVLK PSEITPLSTL RIVELMAEVG FPAGVVNIVP GYGHTAGQRL AEHPGVGKIA FTGSTATGRR IVEASQGNLK RVQLELGGKG ANIVFDDANL DAAINGAAWA IFHNQGQACI AGSRLVLHER IADAFLERFV ALASSIRIGN PLDPNTEMGP LTSKQHLDRV LSYVDVAREQ GGRVLTGGSA PQDPALANGY YVRPTIVEAK HATDRVAQEE VFGPFVTVLR FGSDDEALAI ANATEYGLGS GLWTNDLSRA HRMAARIDAG MCWINCYKRV NPGSPFGGVG KSGYGREMGF EAMHDYTEAR SVWVNVDGNV PPHFKR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa cccaactctt catcgacggg cgcttcgttg acgccgtcga tcgcggcacg atcgacgtgc tgaacccgca cgatggctcc gtcatcacga agatcgcggc tgccaccgcg gcggacgtcg atctggccgt cgacgccgcg acgcgcgcct tcccggcctg gtcggcgatg ccggccgccg agcgcggccg cctgctgctg cgcctggccg atgcgatcga agcgaacacg gaagcgctgg cgcagctcga atcgctggac accggccacc cgattcgcga ttcgcgggcg ctcgacgtgc cgcgcacggc ggcctgcttc cgctatttcg gcggcatggc cgacaagctg caaggctcgg tgataccggt cgacaccggc ttcctgaact acgtgcagcg cgcgccgatc ggcgtggtcg gccagatcgt gccgtggaat tttccgctga tgttcacgag ctggaagatg ggcccggcgc tggcggccgg caacacggtg gtgctcaagc cgtcggaaat cacgccgctg tcgacgttgc gcatcgtcga gctgatggcc gaggtcggat ttcctgccgg cgtcgtcaac atcgtgcccg gctacggcca tacggccggc cagcggctcg ccgaacatcc gggcgtcggc aagatcgcgt tcaccggctc gacggcgacc ggccggcgaa tcgtcgaagc atcgcagggc aacctgaagc gcgtgcaact ggaactgggc ggcaagggcg ccaacatcgt gttcgacgat gccaacctcg acgcggccat caacggcgcc gcgtgggcga tcttccacaa ccaggggcag gcgtgtatcg cggggtcgcg tctggtgctg cacgaacgca tcgcggacgc gtttctcgaa cgcttcgtcg cgctcgcgtc gtcgatccgg atcggcaatc cgctcgaccc gaataccgaa atggggccgc tcacgtcgaa gcagcacctc gatcgcgtgc tgtcgtatgt cgacgtcgcg cgcgagcagg gcggccgcgt gctgacgggc ggcagcgcgc cgcaggaccc ggcgctcgcc aatggctatt acgtgcgccc gacgatcgtc gaggcgaagc atgcgaccga ccgcgtcgcg caggaagaag tgttcggccc gttcgtcacc gtgctgcgct tcggcagcga cgacgaagcg ctggccatcg ccaacgcgac ggaatacggg ctcggcagcg gcctgtggac caacgacctg tcgcgcgccc accggatggc ggcgcggatc gacgcgggca tgtgctggat caactgctac aagcgcgtga atccgggcag cccgttcggc ggcgtcggca agtcgggcta cggccgcgag atgggcttcg aggcgatgca cgactacacg gaagcgcgct cggtgtgggt caacgtcgac ggcaacgtgc cgccgcattt caagcgctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BuceA.00020.y.B1.PS01792
| PURIFICATION DATe: | 4/3/2013 |
| CONCENTRATION: | 48mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 84 |
Protocol Notes
notes unavailable
Validated AA Sequence
MPAAERGRLL LRLADAIEAN TEALAQLESL DTGHPIRDSR ALDVPRTAAC FRYFGGMADK LQGSVIPVDT GFLNYVQRAP IGVVGQIVPW NFPLMFTSWK MGPALAAGNT VVLKPSEITP LSTLRIVELM AEVGFPAGVV NIVPGYGHTA GQRLAEHPGV GKIAFTGSTA TGRRIVEASQ GNLKRVQLEL GGKGANIVFD DANLDAAING AAWAIFHNQG QACIAGSRLV LHERIADAFL ERFVALASSI RIGNPLDPNT EMGPLTXKQH LDRVLXYVDV AREQGGRVLT GGSAPQDPAL ANGYYVRPTI VEAKHATDRV AQEEVFGPFV TVLRFGSDDE ALAIANATEY GLGSGLWTND LSRAHRMAAR IDAGMCWINC YKRVNPGSPF GGVGKSGYGR EMGFEAMHDY TEARSVWVNV D
Validated NT Sequence
gccgtcgacg ttgacccaca ccgagcgcgc ttccgtgtag tcgtgcatcg cctcgaagcc catctcgcgg ccgtagcccg acttgccgac gccgccgaac gggctgcccg gattcacgcg cttgtagcag ttgatccagc acatgcccgc gtcgatccgc gccgccatcc ggtgggcgcg cgacaggtcg ttggtccaca ggccgctgcc gagcccgtat tccgtcgcgt tggcgatggc cagcgcttcg tcgtcgctgc cgaagcgcag cacggtgacg aacgggccga acacttcttc ctgcgcgacg cggtcggtcg catgcttcgc ctcgacgatc gtcgggcgca cgtaatancc attggcgagc gccgggtcct gcggcgcgct gccgcccgtc agcacgcggc cgccctgctc gcgcgcgacg tcgacatacn acagcacgcg atcgaggtgc tgcttcnacg tgagcggccc catttcggta ttcgggtcga gcggattgcc gatccggatc gacgacgcga gcgcgacgaa gcgttcgaga aacgcgtccg cgatgcgttc gtgcagcacc agacgcgacc ccgcgataca cgcctgcccc tggttgtgga agatcgccca cgcggcgccg ttgatggccg cgtcgaggtt ggcatcgtcg aacacgatgt tggcgccctt gccgcccagt tccagttgca cgcgcttcag gttgccctgc gatgcttcga cgattcgccg gccggtcgcc gtcgagccgg tgaacgcgat cttgccgacg cccggatgtt cggcgagccg ctggccggcc gtatggccgt agccgggcac gatgttgacg acgccggcag gaaatccgac ctcggccatc agctcgacga tgcgcaacgt cgacagcggc gtgatttccg acggcttgag caccaccgtg ttgccggccg ccagcgccgg gcccatcttc cagctcgtga acatcagcgg aaaattccac ggcacgatct ggccgaccac gccgatcggc gcgcgctgca cgtagttcag gaagccggtg tcgaccggta tcaccgagcc ttgcagcttg tcggccatgc cgccgaaata gcggaagcag gccgccgtgc gcggcacgtc gagcgcccgc gaatcgcgaa tcgggtggcc ggtgtccagc gattcgagct gcgccagcgc ttccgtgttc gcttcgatcg catcggccag gcgcagcagc aggcggccgc gctcggcggc cggcatcgcc gaccaggccg ggaaggcgcg cgtcgcggcg tcgacggcca gatcgacgtc cgccgcggtg gcagccgcga tcttcgtgat gacggagcca tcgtgcgggt tcagcacgtc gatcgtgccg cgatcgacgg cgtcaacgaa gcgcccgtcg atgaagagtt gggttt
Expressed Protein Sequence
MAHHHHHHMQ TQLFIDGRFV DAVDRGTIDV LNPHDGSVIT KIAAATAADV DLAVDAATRA FPAWSAMPAA ERGRLLLRLA DAIEANTEAL AQLESLDTGH PIRDSRALDV PRTAACFRYF GGMADKLQGS VIPVDTGFLN YVQRAPIGVV GQIVPWNFPL MFTSWKMGPA LAAGNTVVLK PSEITPLSTL RIVELMAEVG FPAGVVNIVP GYGHTAGQRL AEHPGVGKIA FTGSTATGRR IVEASQGNLK RVQLELGGKG ANIVFDDANL DAAINGAAWA IFHNQGQACI AGSRLVLHER IADAFLERFV ALASSIRIGN PLDPNTEMGP LTSKQHLDRV LSYVDVAREQ GGRVLTGGSA PQDPALANGY YVRPTIVEAK HATDRVAQEE VFGPFVTVLR FGSDDEALAI ANATEYGLGS GLWTNDLSRA HRMAARIDAG MCWINCYKRV NPGSPFGGVG KSGYGREMGF EAMHDYTEAR SVWVNVDGNV PPHFKR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAA CCCAACTCTT CATCGACGGG CGCTTCGTTG ACGCCGTCGA TCGCGGCACG ATCGACGTGC TGAACCCGCA CGATGGCTCC GTCATCACGA AGATCGCGGC TGCCACCGCG GCGGACGTCG ATCTGGCCGT CGACGCCGCG ACGCGCGCCT TCCCGGCCTG GTCGGCGATG CCGGCCGCCG AGCGCGGCCG CCTGCTGCTG CGCCTGGCCG ATGCGATCGA AGCGAACACG GAAGCGCTGG CGCAGCTCGA ATCGCTGGAC ACCGGCCACC CGATTCGCGA TTCGCGGGCG CTCGACGTGC CGCGCACGGC GGCCTGCTTC CGCTATTTCG GCGGCATGGC CGACAAGCTG CAAGGCTCGG TGATACCGGT CGACACCGGC TTCCTGAACT ACGTGCAGCG CGCGCCGATC GGCGTGGTCG GCCAGATCGT GCCGTGGAAT TTTCCGCTGA TGTTCACGAG CTGGAAGATG GGCCCGGCGC TGGCGGCCGG CAACACGGTG GTGCTCAAGC CGTCGGAAAT CACGCCGCTG TCGACGTTGC GCATCGTCGA GCTGATGGCC GAGGTCGGAT TTCCTGCCGG CGTCGTCAAC ATCGTGCCCG GCTACGGCCA TACGGCCGGC CAGCGGCTCG CCGAACATCC GGGCGTCGGC AAGATCGCGT TCACCGGCTC GACGGCGACC GGCCGGCGAA TCGTCGAAGC ATCGCAGGGC AACCTGAAGC GCGTGCAACT GGAACTGGGC GGCAAGGGCG CCAACATCGT GTTCGACGAT GCCAACCTCG ACGCGGCCAT CAACGGCGCC GCGTGGGCGA TCTTCCACAA CCAGGGGCAG GCGTGTATCG CGGGGTCGCG TCTGGTGCTG CACGAACGCA TCGCGGACGC GTTTCTCGAA CGCTTCGTCG CGCTCGCGTC GTCGATCCGG ATCGGCAATC CGCTCGACCC GAATACCGAA ATGGGGCCGC TCACGTCGAA GCAGCACCTC GATCGCGTGC TGTCGTATGT CGACGTCGCG CGCGAGCAGG GCGGCCGCGT GCTGACGGGC GGCAGCGCGC CGCAGGACCC GGCGCTCGCC AATGGCTATT ACGTGCGCCC GACGATCGTC GAGGCGAAGC ATGCGACCGA CCGCGTCGCG CAGGAAGAAG TGTTCGGCCC GTTCGTCACC GTGCTGCGCT TCGGCAGCGA CGACGAAGCG CTGGCCATCG CCAACGCGAC GGAATACGGG CTCGGCAGCG GCCTGTGGAC CAACGACCTG TCGCGCGCCC ACCGGATGGC GGCGCGGATC GACGCGGGCA TGTGCTGGAT CAACTGCTAC AAGCGCGTGA ATCCGGGCAG CCCGTTCGGC GGCGTCGGCA AGTCGGGCTA CGGCCGCGAG ATGGGCTTCG AGGCGATGCA CGACTACACG GAAGCGCGCT CGGTGTGGGT CAACGTCGAC GGCAACGTGC CGCCGCATTT CAAGCGCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT