SSGCID: Target Page

BuceA.00020.x

Putative succinate-semialdehyde dehydrogenase [NADP+]

CENTER ID:	BuceA.00020.x
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00020.x.B1.GE36716	Full length( BuceA.00020.x )		1	489

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.6375
RefSeq:	YP_002235165.1
UniProt:	B4EJR1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTVQETLAL KDPSLFRQQA YVNGEWQGAT NGETFDVRNP ATGGLLGTVP AMGAAETRHA IDAANAAWPA WRKKTAKERA VILRKWHDLM MENADDLALI LTTEQGKSLA EAKGEIGYAA SFLEWFAEEG KRVYGDTIPT PASDKRIVVT KEAIGVCAAI TPWNFPAAMI TRKVGPALAA GCPIVVKPAE ATPFSALAMA VLAERAGVPA GVFSVVTGDP KAIGGELTSN PIVRKLSFTG STPVGRLLMA QCAATVKKVS LELGGNAPFI VFDDADLDAA VQGAIASKYR NSGQTCVCTN RFYVHEAVYD QFAQKLAAAV GQLKVGRGTE PGVTQGPLIN EAAVLKVEAH IEDALAKGAT VVTGGKRHAL GHGFFEPTVL TGVTPAMKVA KEETFGPLAP LFKFGSDDEV IRLANDTEFG LAAYFYSRDI GRVWKVAEAL EYGMVGVNTG LISNEVAPFG GVKQSGLGRE GSHYGIDDYV VIKYLCLAV

NT Sequence

ATGAGCACTG TTCAGGAAAC CCTGGCACTG AAAGATCCGT CGCTGTTCCG CCAGCAGGCG TACGTCAACG GCGAATGGCA AGGCGCGACG AACGGCGAGA CGTTCGACGT CCGCAACCCG GCCACCGGCG GCCTGCTCGG CACCGTGCCG GCGATGGGCG CGGCCGAGAC ACGTCACGCG ATCGACGCCG CGAACGCCGC CTGGCCGGCA TGGCGCAAGA AGACCGCGAA GGAGCGTGCG GTCATCCTGC GCAAGTGGCA CGACCTGATG ATGGAAAACG CCGACGACCT CGCGCTGATC CTGACGACCG AGCAAGGCAA GTCGCTGGCC GAAGCGAAGG GCGAGATCGG CTACGCGGCG TCGTTCCTCG AATGGTTCGC CGAGGAAGGC AAGCGCGTGT ACGGCGACAC GATCCCGACG CCGGCGAGCG ACAAGCGCAT CGTCGTGACG AAGGAAGCGA TCGGCGTATG CGCGGCGATC ACGCCGTGGA ACTTCCCGGC GGCGATGATC ACGCGCAAGG TCGGCCCGGC GCTCGCGGCC GGCTGCCCGA TCGTCGTGAA GCCGGCCGAG GCCACGCCGT TCTCCGCGCT CGCGATGGCG GTGCTCGCCG AGCGCGCGGG CGTGCCGGCC GGCGTGTTCA GCGTCGTCAC GGGCGACCCG AAGGCGATCG GCGGCGAGCT GACGTCGAAC CCGATCGTGC GCAAGCTGTC GTTCACGGGC TCGACGCCGG TCGGCCGCCT GCTGATGGCG CAATGCGCGG CGACGGTCAA GAAGGTGTCG CTGGAGCTCG GCGGCAACGC GCCGTTCATC GTGTTCGACG ACGCCGATCT CGATGCGGCC GTGCAGGGCG CGATCGCGTC GAAGTACCGC AACAGCGGCC AGACGTGCGT GTGCACGAAC CGCTTCTACG TGCACGAAGC CGTATACGAC CAGTTCGCGC AGAAGCTCGC GGCGGCCGTC GGCCAGCTGA AGGTCGGCCG CGGCACGGAG CCGGGCGTCA CGCAGGGCCC GCTGATCAAC GAAGCGGCCG TGCTGAAGGT CGAGGCGCAC ATCGAGGACG CGCTCGCGAA GGGCGCGACC GTCGTGACGG GCGGCAAGCG CCATGCGCTC GGCCACGGCT TCTTCGAGCC GACCGTGCTG ACGGGCGTCA CGCCGGCGAT GAAGGTCGCG AAGGAAGAAA CGTTCGGGCC GCTCGCGCCG CTGTTCAAGT TCGGCAGCGA CGACGAAGTG ATCCGCCTCG CGAACGACAC CGAGTTCGGC CTCGCCGCGT ACTTCTACAG CCGCGACATC GGTCGCGTGT GGAAGGTGGC CGAAGCGCTC GAATACGGGA TGGTCGGCGT GAACACGGGC CTGATCTCGA ACGAAGTCGC GCCGTTCGGC GGCGTCAAGC AGTCGGGCCT CGGCCGCGAA GGCTCGCACT ACGGCATCGA CGACTACGTC GTGATCAAGT ACCTCTGCCT GGCCGTC

Details for BuceA.00020.x.B1.GE36716

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	54kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	80
PERCENT COVERAGE:	67

Validated AA Sequence

Validated NT Sequence

tannnnnnnt tnntnnnttt aagaaggaga nataccatgg ctcaccacca ccaccaccat atgagcactg ttcaggaaac cctggcactg aaagatccgt cgctgttccg ccagcaggcg tacgtcaacg gcgaatggca aggcgcgacg aacggcgaga cgttcgacgt ccgcaacccg gccaccggcg gcctgctcgg caccgtgccg gcgatgggcg cggccgagac acgtcacgcg atcgacgccg cgaacgccgc ctggccggca tggcgcaaga agaccgcgaa ggagcgtgcg gtcatcctgc gcaagtggca cgacctgatg atggaaaacg ccgacgacct cgcgctgatc ctgacgaccg agcaaggcaa gtcgctggcc gaagcgaagg gcgagatcgg ctacgcggcg tcgttcctcg aatggttcgc cgaggaaggc aagcgcgtgt acggcgacac gatcccgacg ccggcgagcg acaagcgcat cgtcgtgacg aaggaagcga tcggcgtatg cgcggcgatc acgccgtgga acttcccggc ggcgatgatc acgcgcaagg tcggcccggc gctcgcggcc ggctgcccga tcgtcgtgaa gccggccgag gccacgccgt tctccgcgct cgcgatggcg gtgctcgccg agcgcgcggg cgtgccggcc ggcgtgttca gcgtcgtcac gggcgacccg aaggcgatcg gcggcgagct gacgtcgaac ccgatcgtgc gcaagctgtc gttcacgggc tcgacgccng tcggccgcct gctgatggcg caatgcgcgg cgacngtcaa gagtcgctgg agctcggcgg caacgcgccg ttcatcgtgt tcgacgacgc catctcgatg cggccgtgca gggcgcgatc gcntcgaagt accgcaacag cggccagacg tgcgtgtgca cgaaccgntt ctacgtgcac gaagccgtat acnaccagtt cgngcanaan ctcgcggcnn nngncggcca gctnnnntcg cnnnngnnng nancnngnnn nnnnnnnng

Expected Protein Sequence

MAHHHHHHMS TVQETLALKD PSLFRQQAYV NGEWQGATNG ETFDVRNPAT GGLLGTVPAM GAAETRHAID AANAAWPAWR KKTAKERAVI LRKWHDLMME NADDLALILT TEQGKSLAEA KGEIGYAASF LEWFAEEGKR VYGDTIPTPA SDKRIVVTKE AIGVCAAITP WNFPAAMITR KVGPALAAGC PIVVKPAEAT PFSALAMAVL AERAGVPAGV FSVVTGDPKA IGGELTSNPI VRKLSFTGST PVGRLLMAQC AATVKKVSLE LGGNAPFIVF DDADLDAAVQ GAIASKYRNS GQTCVCTNRF YVHEAVYDQF AQKLAAAVGQ LKVGRGTEPG VTQGPLINEA AVLKVEAHIE DALAKGATVV TGGKRHALGH GFFEPTVLTG VTPAMKVAKE ETFGPLAPLF KFGSDDEVIR LANDTEFGLA AYFYSRDIGR VWKVAEALEY GMVGVNTGLI SNEVAPFGGV KQSGLGREGS HYGIDDYVVI KYLCLAV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca ctgttcagga aaccctggca ctgaaagatc cgtcgctgtt ccgccagcag gcgtacgtca acggcgaatg gcaaggcgcg acgaacggcg agacgttcga cgtccgcaac ccggccaccg gcggcctgct cggcaccgtg ccggcgatgg gcgcggccga gacacgtcac gcgatcgacg ccgcgaacgc cgcctggccg gcatggcgca agaagaccgc gaaggagcgt gcggtcatcc tgcgcaagtg gcacgacctg atgatggaaa acgccgacga cctcgcgctg atcctgacga ccgagcaagg caagtcgctg gccgaagcga agggcgagat cggctacgcg gcgtcgttcc tcgaatggtt cgccgaggaa ggcaagcgcg tgtacggcga cacgatcccg acgccggcga gcgacaagcg catcgtcgtg acgaaggaag cgatcggcgt atgcgcggcg atcacgccgt ggaacttccc ggcggcgatg atcacgcgca aggtcggccc ggcgctcgcg gccggctgcc cgatcgtcgt gaagccggcc gaggccacgc cgttctccgc gctcgcgatg gcggtgctcg ccgagcgcgc gggcgtgccg gccggcgtgt tcagcgtcgt cacgggcgac ccgaaggcga tcggcggcga gctgacgtcg aacccgatcg tgcgcaagct gtcgttcacg ggctcgacgc cggtcggccg cctgctgatg gcgcaatgcg cggcgacggt caagaaggtg tcgctggagc tcggcggcaa cgcgccgttc atcgtgttcg acgacgccga tctcgatgcg gccgtgcagg gcgcgatcgc gtcgaagtac cgcaacagcg gccagacgtg cgtgtgcacg aaccgcttct acgtgcacga agccgtatac gaccagttcg cgcagaagct cgcggcggcc gtcggccagc tgaaggtcgg ccgcggcacg gagccgggcg tcacgcaggg cccgctgatc aacgaagcgg ccgtgctgaa ggtcgaggcg cacatcgagg acgcgctcgc gaagggcgcg accgtcgtga cgggcggcaa gcgccatgcg ctcggccacg gcttcttcga gccgaccgtg ctgacgggcg tcacgccggc gatgaaggtc gcgaaggaag aaacgttcgg gccgctcgcg ccgctgttca agttcggcag cgacgacgaa gtgatccgcc tcgcgaacga caccgagttc ggcctcgccg cgtacttcta cagccgcgac atcggtcgcg tgtggaaggt ggccgaagcg ctcgaatacg ggatggtcgg cgtgaacacg ggcctgatct cgaacgaagt cgcgccgttc ggcggcgtca agcagtcggg cctcggccgc gaaggctcgc actacggcat cgacgactac gtcgtgatca agtacctctg cctggccgtc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat