BuceA.00020.w

Putative aldehyde dehydrogenase

CENTER ID:	BuceA.00020.w
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	native diffraction-data
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00020.w.B1.GE36505	Full length( BuceA.00020.w )		1	481

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00020.w.B1.PS01789	Full length( BuceA.00020.w )		1	481

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127004
PATRIC ID:	fig\|216591.5.peg.5296
RefSeq:	YP_002234153.1
UniProt:	B4EJN2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MANVTYTDTQ LLIDGEWVDA ASGKTIDVVN PATGKPIGKV AHAGIADLDR ALAAAQRGFE AWRKVPAHER AATMRKAAAL VRERADTIAQ LMTQEQGKPL TEARVEVLSA ADIIEWFADE GRRVYGRIVP PRNLGAQQTV VKEPVGPVAA FTPWNFPVNQ VVRKLSAALA TGCSFLVKAP EETPASPAAL LRAFVDAGVP AGVIGLVYGD PAEISSYLIP HPVIRKVTFT GSTPVGKQLA ALAGQHMKRA TMELGGHAPV IVAEDADVAL AVKAAGGAKF RNAGQVCISP TRFLVHNSIR DEFTRALVKH AEGLKVGNGL EEGTTLGALA NPRRLTAMAS VVDNARKVGA SIETGGERIG AEGNFFAPTV IANVPLEADV FNNEPFGPVA AIRGFDKLEE AIAEANRLPF GLAGYAFTRS FANVHLLTQR LEVGMLWINQ PATPWPEMPF GGVKDSGYGS EGGPEALEPY LVTKSVTVMA V

NT Sequence

ATGGCTAACG TGACTTATAC GGATACGCAA CTGCTGATCG ACGGCGAGTG GGTCGACGCC GCGAGCGGCA AGACGATCGA CGTCGTGAAC CCGGCGACCG GCAAGCCGAT CGGCAAGGTG GCCCACGCGG GCATCGCCGA TCTCGACCGC GCGCTCGCCG CCGCGCAGCG CGGCTTCGAG GCATGGCGCA AGGTGCCCGC GCACGAGCGC GCGGCGACGA TGCGCAAGGC GGCCGCGCTG GTGCGCGAAC GCGCGGACAC GATTGCACAG CTGATGACGC AGGAGCAGGG CAAGCCGCTC ACCGAAGCAC GCGTCGAAGT GCTGTCGGCG GCCGACATCA TCGAATGGTT CGCGGACGAA GGCCGCCGCG TGTACGGCCG GATCGTGCCG CCGCGCAACC TCGGCGCGCA GCAGACGGTC GTGAAGGAGC CGGTCGGCCC GGTCGCGGCG TTCACGCCGT GGAATTTCCC GGTCAACCAG GTCGTGCGCA AGCTGAGCGC CGCGCTCGCG ACCGGCTGTT CGTTCCTCGT GAAGGCGCCG GAAGAAACCC CGGCATCGCC GGCCGCGCTG CTGCGCGCGT TCGTCGATGC GGGCGTGCCG GCGGGCGTGA TCGGCCTCGT GTACGGCGAT CCGGCTGAAA TCTCGTCGTA CCTGATTCCG CACCCGGTGA TCCGCAAGGT CACGTTCACG GGTTCGACGC CGGTCGGCAA GCAACTGGCC GCGCTCGCGG GCCAGCACAT GAAGCGCGCG ACGATGGAAC TGGGCGGCCA TGCACCGGTG ATCGTCGCCG AGGACGCGGA CGTCGCGCTC GCGGTGAAGG CCGCCGGCGG GGCGAAGTTC CGTAACGCCG GGCAGGTGTG CATTTCGCCG ACGCGCTTCC TCGTGCACAA CAGCATCCGC GACGAATTCA CGCGCGCGCT GGTCAAGCAC GCCGAAGGGC TGAAGGTCGG CAACGGCCTC GAGGAAGGCA CGACGCTCGG CGCGCTCGCG AACCCGCGGC GCCTGACCGC GATGGCGTCG GTCGTCGACA ACGCGCGCAA GGTCGGCGCG AGCATCGAAA CCGGCGGCGA GCGGATCGGC GCGGAAGGCA ACTTCTTCGC GCCGACCGTG ATCGCGAACG TGCCGCTCGA AGCGGACGTG TTCAACAACG AGCCGTTCGG CCCCGTTGCC GCGATTCGTG GTTTCGACAA GCTGGAGGAG GCGATCGCCG AGGCGAACCG TCTGCCGTTC GGCCTGGCCG GCTATGCGTT CACGCGTTCG TTCGCGAACG TGCATCTGCT CACGCAGCGC CTCGAAGTCG GGATGCTGTG GATCAACCAG CCGGCGACGC CGTGGCCGGA AATGCCGTTC GGCGGCGTGA AGGATTCCGG CTACGGGTCG GAAGGCGGGC CGGAAGCGCT GGAGCCGTAT CTCGTCACGA AGTCGGTGAC GGTGATGGCC GTC

Details for BuceA.00020.w.B1.GE36505

HARVESTED ON:	1/9/2013
SEQUENCED ON:	1/7/2013
EXPECTED MW:	52kDa
OBSERVED MW:	53kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	21
PERCENT COVERAGE:	86

Validated AA Sequence

MHVRERTRER IAGQAERQTV RLGDRLLQLV ETTNRGNGAE RLVVEHVRFE RHVRDHGRRE EVAFRADPLA AGFDARADLA RVVDDRRHRG QAPRVRERAE RRAFLXAVAD LQPFGVLDQR AREXVADAVV HEEARRRNAH LPGVTELRPA GGLHRERDVR VLGDDHRCMA AQFHRRALHV LARERGQLLA DRRRTRERDL ADHRVRNQVR RDFSRIAVHE ADHARRHARI DERAQQRGRR CRGFFRRLHE ERTAGRERGA QLAHDLVDXE IPRRERRDRA DRLLHDRLLR AEVARRHDPA VHAAAFVREP FDDVGRRQHF DACFGERLAL LLRHQLCNRV RAFAHQRGRL AHRRRALVRG HXAPCLEAAL RGGERAVEIG DARVGHLADR LAGRRVHDVD RLAARGVDPL AVDQQLRIRI SHV

Validated NT Sequence

cgtgacgaga tacggctcca gcgcttccgg cccgccttcc gacccgtagc cggaatcctt cacgccgccg aacggcattt ccggccacgg cgtcgccggc tggttgatcc acagcatccc gacttcgagg cgctgcgtga gcagatgcac gttcgcgaac gaacgcgtga acgcatagcc ggccaggccg aacggcagac ggttcgcctc ggcgatcgcc tcctccagct tgtcgaaacc acgaatcgcg gcaacggggc cgaacggctc gttgttgaac acgtccgctt cgagcggcac gttcgcgatc acggtcggcg cgaagaagtt gccttccgcg ccgatccgct cgccgccggt ttcgatgctc gcgccgacct tgcgcgcgtt gtcgacgacc gacgccatcg cggtcaggcg ccgcgggttc gcgagcgcgc cgagcgtcgt gccttcctcn aggccgttgc cgaccttcag cccttcggcg tgcttgacca gcgcgcgcgt gaattngtcg cggatgctgt tgtgcacgag gaagcgcgtc ggcgaaatgc acacctgccc ggcgttacgg aacttcgccc cgccggcggc cttcaccgcg agcgcgacgt ccgcgtcctc ggcgacgatc accggtgcat ggccgcccag ttccatcgtc gcgcgcttca tgtgctggcc cgcgagcgcg gccagttgct tgccgaccgg cgtcgaaccc gtgaacgtga ccttgcggat caccgggtgc ggaatcaggt acgacgagat ttcagccgga tcgccgtaca cgaggccgat cacgcccgcc ggcacgcccg catcgacgaa cgcgcgcagc agcgcggccg gcgatgccgg ggtttcttcc ggcgccttca cgaggaacga acagccggtc gcgagcgcgg cgctcagctt gcgcacgacc tggttgaccn ggaaattcca cggcgtgaac gccgcgaccg ggccgaccgg ctccttcacg accgtctgct gcgcgccgag gttgcgcggc ggcacgatcc ggccgtacac gcggcggcct tcgtccgcga accattcgat gatgtcggcc gccgacagca cttcgacgcg tgcttcggtg agcggcttgc cctgctcctg cgtcatcagc tgtgcaatcg tgtccgcgcg ttcgcgcacc agcgcggccg ccttgcgcat cgtcgccgcg cgctcgtgcg cgggcaccnt gcgccatgcc tcgaagccgc gctgcgcggc ggcgagcgcg cggtcgagat cggcgatgcc cgcgtgggcc accttgccga tcggcttgcc ggtcgccggg ttcacgacgt cgatcgtctt gccgctcgcg gcgtcgaccc actcgccgtc gatcagcagt tgcgtatccg tataagtcac gttag

Expected Protein Sequence

MAHHHHHHMA NVTYTDTQLL IDGEWVDAAS GKTIDVVNPA TGKPIGKVAH AGIADLDRAL AAAQRGFEAW RKVPAHERAA TMRKAAALVR ERADTIAQLM TQEQGKPLTE ARVEVLSAAD IIEWFADEGR RVYGRIVPPR NLGAQQTVVK EPVGPVAAFT PWNFPVNQVV RKLSAALATG CSFLVKAPEE TPASPAALLR AFVDAGVPAG VIGLVYGDPA EISSYLIPHP VIRKVTFTGS TPVGKQLAAL AGQHMKRATM ELGGHAPVIV AEDADVALAV KAAGGAKFRN AGQVCISPTR FLVHNSIRDE FTRALVKHAE GLKVGNGLEE GTTLGALANP RRLTAMASVV DNARKVGASI ETGGERIGAE GNFFAPTVIA NVPLEADVFN NEPFGPVAAI RGFDKLEEAI AEANRLPFGL AGYAFTRSFA NVHLLTQRLE VGMLWINQPA TPWPEMPFGG VKDSGYGSEG GPEALEPYLV TKSVTVMAV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta acgtgactta tacggatacg caactgctga tcgacggcga gtgggtcgac gccgcgagcg gcaagacgat cgacgtcgtg aacccggcga ccggcaagcc gatcggcaag gtggcccacg cgggcatcgc cgatctcgac cgcgcgctcg ccgccgcgca gcgcggcttc gaggcatggc gcaaggtgcc cgcgcacgag cgcgcggcga cgatgcgcaa ggcggccgcg ctggtgcgcg aacgcgcgga cacgattgca cagctgatga cgcaggagca gggcaagccg ctcaccgaag cacgcgtcga agtgctgtcg gcggccgaca tcatcgaatg gttcgcggac gaaggccgcc gcgtgtacgg ccggatcgtg ccgccgcgca acctcggcgc gcagcagacg gtcgtgaagg agccggtcgg cccggtcgcg gcgttcacgc cgtggaattt cccggtcaac caggtcgtgc gcaagctgag cgccgcgctc gcgaccggct gttcgttcct cgtgaaggcg ccggaagaaa ccccggcatc gccggccgcg ctgctgcgcg cgttcgtcga tgcgggcgtg ccggcgggcg tgatcggcct cgtgtacggc gatccggctg aaatctcgtc gtacctgatt ccgcacccgg tgatccgcaa ggtcacgttc acgggttcga cgccggtcgg caagcaactg gccgcgctcg cgggccagca catgaagcgc gcgacgatgg aactgggcgg ccatgcaccg gtgatcgtcg ccgaggacgc ggacgtcgcg ctcgcggtga aggccgccgg cggggcgaag ttccgtaacg ccgggcaggt gtgcatttcg ccgacgcgct tcctcgtgca caacagcatc cgcgacgaat tcacgcgcgc gctggtcaag cacgccgaag ggctgaaggt cggcaacggc ctcgaggaag gcacgacgct cggcgcgctc gcgaacccgc ggcgcctgac cgcgatggcg tcggtcgtcg acaacgcgcg caaggtcggc gcgagcatcg aaaccggcgg cgagcggatc ggcgcggaag gcaacttctt cgcgccgacc gtgatcgcga acgtgccgct cgaagcggac gtgttcaaca acgagccgtt cggccccgtt gccgcgattc gtggtttcga caagctggag gaggcgatcg ccgaggcgaa ccgtctgccg ttcggcctgg ccggctatgc gttcacgcgt tcgttcgcga acgtgcatct gctcacgcag cgcctcgaag tcgggatgct gtggatcaac cagccggcga cgccgtggcc ggaaatgccg ttcggcggcg tgaaggattc cggctacggg tcggaaggcg ggccggaagc gctggagccg tatctcgtca cgaagtcggt gacggtgatg gccgtctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for BuceA.00020.w.B1.PS01789

PURIFICATION DATe:	3/20/2013
CONCENTRATION:	62.1mg/ml
OBSERVED MW:	55kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	21
PERCENT COVERAGE:	86

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTA ACGTGACTTA TACGGATACG CAACTGCTGA TCGACGGCGA GTGGGTCGAC GCCGCGAGCG GCAAGACGAT CGACGTCGTG AACCCGGCGA CCGGCAAGCC GATCGGCAAG GTGGCCCACG CGGGCATCGC CGATCTCGAC CGCGCGCTCG CCGCCGCGCA GCGCGGCTTC GAGGCATGGC GCAAGGTGCC CGCGCACGAG CGCGCGGCGA CGATGCGCAA GGCGGCCGCG CTGGTGCGCG AACGCGCGGA CACGATTGCA CAGCTGATGA CGCAGGAGCA GGGCAAGCCG CTCACCGAAG CACGCGTCGA AGTGCTGTCG GCGGCCGACA TCATCGAATG GTTCGCGGAC GAAGGCCGCC GCGTGTACGG CCGGATCGTG CCGCCGCGCA ACCTCGGCGC GCAGCAGACG GTCGTGAAGG AGCCGGTCGG CCCGGTCGCG GCGTTCACGC CGTGGAATTT CCCGGTCAAC CAGGTCGTGC GCAAGCTGAG CGCCGCGCTC GCGACCGGCT GTTCGTTCCT CGTGAAGGCG CCGGAAGAAA CCCCGGCATC GCCGGCCGCG CTGCTGCGCG CGTTCGTCGA TGCGGGCGTG CCGGCGGGCG TGATCGGCCT CGTGTACGGC GATCCGGCTG AAATCTCGTC GTACCTGATT CCGCACCCGG TGATCCGCAA GGTCACGTTC ACGGGTTCGA CGCCGGTCGG CAAGCAACTG GCCGCGCTCG CGGGCCAGCA CATGAAGCGC GCGACGATGG AACTGGGCGG CCATGCACCG GTGATCGTCG CCGAGGACGC GGACGTCGCG CTCGCGGTGA AGGCCGCCGG CGGGGCGAAG TTCCGTAACG CCGGGCAGGT GTGCATTTCG CCGACGCGCT TCCTCGTGCA CAACAGCATC CGCGACGAAT TCACGCGCGC GCTGGTCAAG CACGCCGAAG GGCTGAAGGT CGGCAACGGC CTCGAGGAAG GCACGACGCT CGGCGCGCTC GCGAACCCGC GGCGCCTGAC CGCGATGGCG TCGGTCGTCG ACAACGCGCG CAAGGTCGGC GCGAGCATCG AAACCGGCGG CGAGCGGATC GGCGCGGAAG GCAACTTCTT CGCGCCGACC GTGATCGCGA ACGTGCCGCT CGAAGCGGAC GTGTTCAACA ACGAGCCGTT CGGCCCCGTT GCCGCGATTC GTGGTTTCGA CAAGCTGGAG GAGGCGATCG CCGAGGCGAA CCGTCTGCCG TTCGGCCTGG CCGGCTATGC GTTCACGCGT TCGTTCGCGA ACGTGCATCT GCTCACGCAG CGCCTCGAAG TCGGGATGCT GTGGATCAAC CAGCCGGCGA CGCCGTGGCC GGAAATGCCG TTCGGCGGCG TGAAGGATTC CGGCTACGGG TCGGAAGGCG GGCCGGAAGC GCTGGAGCCG TATCTCGTCA CGAAGTCGGT GACGGTGATG GCCGTCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T