BuceA.00020.t

Putative NADP-dependent glyceraldehyde-3-phosphate dehydrogenase

CENTER ID: BuceA.00020.t
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00020.t.B1.GE36502 Full length( BuceA.00020.t ) 1 483
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00020.t.B1.PS01810 Full length( BuceA.00020.t ) 1 483

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.6302
RefSeq: YP_002235096.1
UniProt: B4EJ46

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAHPRQDHPS FRAEALRWCG TRAMRERTLD VTDPYTGTRV GTAPLASVDD VRAAFDYAMA YRPTLTRYER SQILERAAAL LRERTEEASD LITLESGLSK QDSRYEIGRV ADVLKFAAVE ALRDDAQSFS CDLTPHGKAR RVFSQRQPLD GVIVAITPFN HPMNQVAHKI APAIATNNRV IVKPSEKVPL SAFYLADLLY EAGLPEPMLQ VLTGDPAEIA DELVTHPHAS LITFTGGVAI GKAIAARAGY RRIVLELGGN DPLIVLADAD PDRAASLAVL GSYRNSGQRC TAVKRMLVQR SIVPAFTELL VEKTRAWKYG DPFDPSNEMG TVIDEAAARL FEARVNEAVA QGARLLTGNV RRGALYAPTV LDNVDPSMTL VREETFGPVS PVIAFDTIDD AIRLSNGTAF GLSSGVCTDS TAAVVRFVNE LNVGTVNVWE VPGYRIELSP FGGIKDSGLG YKEGVQEAMK SFTNLKTFSL PWE
NT Sequence
ATGGCTCATC CGCGGCAAGA TCATCCGTCG TTTCGTGCCG AAGCGTTGCG TTGGTGCGGC ACGCGCGCGA TGCGCGAGCG CACGCTCGAC GTCACGGACC CGTACACGGG CACGCGCGTC GGCACCGCGC CGCTCGCCAG CGTCGACGAC GTGCGTGCGG CGTTCGATTA CGCGATGGCC TACCGGCCGA CGCTGACGCG CTACGAACGC TCGCAGATTC TCGAACGTGC GGCCGCGCTG CTGCGCGAGC GCACCGAGGA GGCGTCCGAC CTGATCACGC TCGAGTCGGG GTTGTCGAAA CAGGATTCGC GCTACGAGAT CGGCCGGGTG GCCGACGTGC TGAAGTTCGC GGCGGTCGAG GCGCTGCGTG ACGATGCGCA GAGTTTCTCT TGCGACCTGA CGCCCCACGG CAAGGCGCGG CGCGTGTTCT CGCAGCGCCA GCCGCTCGAC GGCGTGATCG TCGCGATTAC GCCGTTCAAT CATCCGATGA ACCAGGTCGC GCACAAGATC GCGCCGGCGA TCGCGACCAA CAACCGCGTG ATCGTGAAGC CGTCGGAGAA GGTGCCGCTG TCGGCGTTCT ATCTGGCCGA CCTGCTGTAT GAAGCCGGGC TGCCGGAACC GATGCTGCAG GTGTTGACCG GCGACCCGGC CGAGATCGCG GACGAGCTCG TCACGCATCC GCATGCGTCG CTGATCACGT TCACGGGCGG CGTCGCGATC GGCAAGGCGA TTGCGGCGCG GGCCGGCTAC CGGCGCATCG TGCTGGAGCT GGGCGGCAAC GATCCGCTGA TCGTCCTCGC CGATGCCGAT CCCGACCGCG CGGCGTCGCT CGCGGTGTTG GGCTCGTACC GGAATTCGGG CCAGCGCTGC ACGGCCGTCA AGCGGATGCT GGTGCAGCGC TCCATCGTGC CGGCGTTCAC CGAATTGCTC GTCGAGAAGA CGCGCGCCTG GAAATATGGC GATCCATTCG ATCCCTCGAA CGAAATGGGC ACGGTGATCG ACGAAGCCGC GGCGCGGCTG TTCGAGGCGC GCGTGAACGA GGCGGTCGCG CAGGGCGCGC GCCTGCTGAC GGGCAATGTG CGGCGCGGCG CGTTGTACGC GCCGACCGTG CTCGACAACG TCGATCCGTC GATGACGCTG GTGCGCGAGG AGACCTTCGG GCCCGTGTCG CCCGTCATCG CATTCGATAC GATCGACGAC GCGATCCGGC TCAGCAACGG CACGGCGTTC GGGCTGTCGT CGGGTGTCTG TACGGACAGC ACGGCCGCCG TCGTACGGTT CGTGAACGAG TTGAACGTCG GCACGGTGAA CGTATGGGAA GTGCCCGGAT ACCGGATCGA ACTGTCGCCG TTCGGCGGGA TCAAGGATTC GGGGCTCGGT TACAAGGAAG GCGTCCAGGA GGCGATGAAG AGTTTCACGA ACCTGAAGAC GTTTTCGCTG CCTTGGGAG
Details for BuceA.00020.t.B1.GE36502
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/7/2013
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 91
Validated AA Sequence
MRERTLDVTD PYTGTRVGTA PLASVDDVRA AFDYAMAYRP TLTRYERSQI LERAAALLRE RTEEASDLIT LESGLSKQDS RYEIGRVADV LKFAAVEALR DDAQSFSCDL TPHGKARRVF SQRQPLDGVI VAITPFNHPM NQVAHKIAPA IATNNRVIVK PSEKVPLSAF YLADLLYEAG LPEPMLQVLT GDPAEIADEL VTHPHASLIT FTGGVAIGKA IAARAGYRRI VLELGGNDPL IVLADADPDR AASLAVLGSY RNSGQRCTAV KRMLVQRSIV PAFTELLVEK TRAWKYGDPF DPSNEMGTVI XEAAARLFEA RVNEAVAQGA RLLTGNVRRG ALYAPTVLDN VDPSMTLVRE ETFGPVSPVI AFDTIDDAIR LSNGTAFGLS SGVCTDSTAA VVRFVNELNV GTVNVWEVPG YRIELSPFGG IKDSGLGYKE GVQEAMKSFT N
Validated NT Sequence
aggttcgtga aactcttcat cgcctcctgg acgccttcct tgtaaccgag ccccgaatcc ttgatcccgc cgaacggcga cagttcgatc cggtatccgg gcacttccca tacgttcacc gtgccgacgt tcaactcgtt cacgaaccgt acgacggcgg ccgtgctgtc cgtacagaca cccgacgaca gcccgaacgc cgtgccgttg ctgagccgga tcgcgtcgtc gatcgtatcg aatgcgatga cgggcgacac gggcccgaag gtctcctcgc gcaccagcgt catcgacgga tcgacgttgt cgagcacggt cggcgcgtac aacgcgccgc gccgcacatt gcccgtcagc aggcgcgcgc cctgcgcgac cgcctcgttc acgcgcgcct cgaacagccg cgccgcggct tcntcgatca ccgtgcccat ttcgttcgag ggatcgaatg gatcgccata tttccaggcg cgcgtcttct cgacgagcaa ttcggtgaac gccggcacga tggagcgctg caccagcatc cgcttgacgg ccgtgcagcg ctggcccgaa ttccggtacg agcccaacac cgcgagcgac gccgcgcggt cgggatcggc atcggcgagg acgatcagcg gatcgttgcc gcccagctcc agcacgatgc gccggtagcc ggcccgcgcc gcaatcgcct tgccgatcgc gacgccgccc gtgaacgtga tcagcgacgc atgcggatgc gtgacgagct cgtccgcgat ctcggccggg tcgccggtca acacctgcag catcggttcc ggcagcccgg cttcatacag caggtcggcc agatagaacg ccgacagcgg caccttctcc gacggcttca cgatcacgcg gttgttggtc gcgatcgccg gcgcgatctt gtgcgcgacc tggttcatcg gatgattgaa cggcgtaatc gcgacgatca cgccgtcgag cggctggcgc tgcgagaaca cgcgccgcgc cttgccgtgg ggcgtcaggt cgcaagagaa actctgcgca tcgtcacgca gcgcctcgac cgccgcgaac ttcagcacgt cggccacccg gccgatctcg tagcgcgaat cctgtttcga caaccccgac tcgagcgtga tcaggtcgga cgcctcctcg gtgcgctcgc gcagcagcgc ggccgcacgt tcgagaatct gcgagcgttc gtagcgcgtc agcgtcggcc ggtaggccat cgcgtaatcg aacgccgcac gcacgtcgtc gacgctggcg agcggcgcgg tgccgacgcg cgtgcccgtg tacgggtccg tgacgtcgag cgtgcgctcg cgcatcgcgc gcgtgccgca ccaacgcaac gcttcggcac gaaacgacgg atgatcttgc cgcggatgag
Expected Protein Sequence
MAHHHHHHMA HPRQDHPSFR AEALRWCGTR AMRERTLDVT DPYTGTRVGT APLASVDDVR AAFDYAMAYR PTLTRYERSQ ILERAAALLR ERTEEASDLI TLESGLSKQD SRYEIGRVAD VLKFAAVEAL RDDAQSFSCD LTPHGKARRV FSQRQPLDGV IVAITPFNHP MNQVAHKIAP AIATNNRVIV KPSEKVPLSA FYLADLLYEA GLPEPMLQVL TGDPAEIADE LVTHPHASLI TFTGGVAIGK AIAARAGYRR IVLELGGNDP LIVLADADPD RAASLAVLGS YRNSGQRCTA VKRMLVQRSI VPAFTELLVE KTRAWKYGDP FDPSNEMGTV IDEAAARLFE ARVNEAVAQG ARLLTGNVRR GALYAPTVLD NVDPSMTLVR EETFGPVSPV IAFDTIDDAI RLSNGTAFGL SSGVCTDSTA AVVRFVNELN VGTVNVWEVP GYRIELSPFG GIKDSGLGYK EGVQEAMKSF TNLKTFSLPW E
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctc atccgcggca agatcatccg tcgtttcgtg ccgaagcgtt gcgttggtgc ggcacgcgcg cgatgcgcga gcgcacgctc gacgtcacgg acccgtacac gggcacgcgc gtcggcaccg cgccgctcgc cagcgtcgac gacgtgcgtg cggcgttcga ttacgcgatg gcctaccggc cgacgctgac gcgctacgaa cgctcgcaga ttctcgaacg tgcggccgcg ctgctgcgcg agcgcaccga ggaggcgtcc gacctgatca cgctcgagtc ggggttgtcg aaacaggatt cgcgctacga gatcggccgg gtggccgacg tgctgaagtt cgcggcggtc gaggcgctgc gtgacgatgc gcagagtttc tcttgcgacc tgacgcccca cggcaaggcg cggcgcgtgt tctcgcagcg ccagccgctc gacggcgtga tcgtcgcgat tacgccgttc aatcatccga tgaaccaggt cgcgcacaag atcgcgccgg cgatcgcgac caacaaccgc gtgatcgtga agccgtcgga gaaggtgccg ctgtcggcgt tctatctggc cgacctgctg tatgaagccg ggctgccgga accgatgctg caggtgttga ccggcgaccc ggccgagatc gcggacgagc tcgtcacgca tccgcatgcg tcgctgatca cgttcacggg cggcgtcgcg atcggcaagg cgattgcggc gcgggccggc taccggcgca tcgtgctgga gctgggcggc aacgatccgc tgatcgtcct cgccgatgcc gatcccgacc gcgcggcgtc gctcgcggtg ttgggctcgt accggaattc gggccagcgc tgcacggccg tcaagcggat gctggtgcag cgctccatcg tgccggcgtt caccgaattg ctcgtcgaga agacgcgcgc ctggaaatat ggcgatccat tcgatccctc gaacgaaatg ggcacggtga tcgacgaagc cgcggcgcgg ctgttcgagg cgcgcgtgaa cgaggcggtc gcgcagggcg cgcgcctgct gacgggcaat gtgcggcgcg gcgcgttgta cgcgccgacc gtgctcgaca acgtcgatcc gtcgatgacg ctggtgcgcg aggagacctt cgggcccgtg tcgcccgtca tcgcattcga tacgatcgac gacgcgatcc ggctcagcaa cggcacggcg ttcgggctgt cgtcgggtgt ctgtacggac agcacggccg ccgtcgtacg gttcgtgaac gagttgaacg tcggcacggt gaacgtatgg gaagtgcccg gataccggat cgaactgtcg ccgttcggcg ggatcaagga ttcggggctc ggttacaagg aaggcgtcca ggaggcgatg aagagtttca cgaacctgaa gacgttttcg ctgccttggg agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BuceA.00020.t.B1.PS01810
PURIFICATION DATe: 5/2/2013
CONCENTRATION: 18mg/ml
OBSERVED MW: 54kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 91
Protocol Notes
notes unavailable
Validated AA Sequence
MRERTLDVTD PYTGTRVGTA PLASVDDVRA AFDYAMAYRP TLTRYERSQI LERAAALLRE RTEEASDLIT LESGLSKQDS RYEIGRVADV LKFAAVEALR DDAQSFSCDL TPHGKARRVF SQRQPLDGVI VAITPFNHPM NQVAHKIAPA IATNNRVIVK PSEKVPLSAF YLADLLYEAG LPEPMLQVLT GDPAEIADEL VTHPHASLIT FTGGVAIGKA IAARAGYRRI VLELGGNDPL IVLADADPDR AASLAVLGSY RNSGQRCTAV KRMLVQRSIV PAFTELLVEK TRAWKYGDPF DPSNEMGTVI XEAAARLFEA RVNEAVAQGA RLLTGNVRRG ALYAPTVLDN VDPSMTLVRE ETFGPVSPVI AFDTIDDAIR LSNGTAFGLS SGVCTDSTAA VVRFVNELNV GTVNVWEVPG YRIELSPFGG IKDSGLGYKE GVQEAMKSFT N
Validated NT Sequence
aggttcgtga aactcttcat cgcctcctgg acgccttcct tgtaaccgag ccccgaatcc ttgatcccgc cgaacggcga cagttcgatc cggtatccgg gcacttccca tacgttcacc gtgccgacgt tcaactcgtt cacgaaccgt acgacggcgg ccgtgctgtc cgtacagaca cccgacgaca gcccgaacgc cgtgccgttg ctgagccgga tcgcgtcgtc gatcgtatcg aatgcgatga cgggcgacac gggcccgaag gtctcctcgc gcaccagcgt catcgacgga tcgacgttgt cgagcacggt cggcgcgtac aacgcgccgc gccgcacatt gcccgtcagc aggcgcgcgc cctgcgcgac cgcctcgttc acgcgcgcct cgaacagccg cgccgcggct tcntcgatca ccgtgcccat ttcgttcgag ggatcgaatg gatcgccata tttccaggcg cgcgtcttct cgacgagcaa ttcggtgaac gccggcacga tggagcgctg caccagcatc cgcttgacgg ccgtgcagcg ctggcccgaa ttccggtacg agcccaacac cgcgagcgac gccgcgcggt cgggatcggc atcggcgagg acgatcagcg gatcgttgcc gcccagctcc agcacgatgc gccggtagcc ggcccgcgcc gcaatcgcct tgccgatcgc gacgccgccc gtgaacgtga tcagcgacgc atgcggatgc gtgacgagct cgtccgcgat ctcggccggg tcgccggtca acacctgcag catcggttcc ggcagcccgg cttcatacag caggtcggcc agatagaacg ccgacagcgg caccttctcc gacggcttca cgatcacgcg gttgttggtc gcgatcgccg gcgcgatctt gtgcgcgacc tggttcatcg gatgattgaa cggcgtaatc gcgacgatca cgccgtcgag cggctggcgc tgcgagaaca cgcgccgcgc cttgccgtgg ggcgtcaggt cgcaagagaa actctgcgca tcgtcacgca gcgcctcgac cgccgcgaac ttcagcacgt cggccacccg gccgatctcg tagcgcgaat cctgtttcga caaccccgac tcgagcgtga tcaggtcgga cgcctcctcg gtgcgctcgc gcagcagcgc ggccgcacgt tcgagaatct gcgagcgttc gtagcgcgtc agcgtcggcc ggtaggccat cgcgtaatcg aacgccgcac gcacgtcgtc gacgctggcg agcggcgcgg tgccgacgcg cgtgcccgtg tacgggtccg tgacgtcgag cgtgcgctcg cgcatcgcgc gcgtgccgca ccaacgcaac gcttcggcac gaaacgacgg atgatcttgc cgcggatgag
Expressed Protein Sequence
MAHHHHHHMA HPRQDHPSFR AEALRWCGTR AMRERTLDVT DPYTGTRVGT APLASVDDVR AAFDYAMAYR PTLTRYERSQ ILERAAALLR ERTEEASDLI TLESGLSKQD SRYEIGRVAD VLKFAAVEAL RDDAQSFSCD LTPHGKARRV FSQRQPLDGV IVAITPFNHP MNQVAHKIAP AIATNNRVIV KPSEKVPLSA FYLADLLYEA GLPEPMLQVL TGDPAEIADE LVTHPHASLI TFTGGVAIGK AIAARAGYRR IVLELGGNDP LIVLADADPD RAASLAVLGS YRNSGQRCTA VKRMLVQRSI VPAFTELLVE KTRAWKYGDP FDPSNEMGTV IDEAAARLFE ARVNEAVAQG ARLLTGNVRR GALYAPTVLD NVDPSMTLVR EETFGPVSPV IAFDTIDDAI RLSNGTAFGL SSGVCTDSTA AVVRFVNELN VGTVNVWEVP GYRIELSPFG GIKDSGLGYK EGVQEAMKSF TNLKTFSLPW E
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTC ATCCGCGGCA AGATCATCCG TCGTTTCGTG CCGAAGCGTT GCGTTGGTGC GGCACGCGCG CGATGCGCGA GCGCACGCTC GACGTCACGG ACCCGTACAC GGGCACGCGC GTCGGCACCG CGCCGCTCGC CAGCGTCGAC GACGTGCGTG CGGCGTTCGA TTACGCGATG GCCTACCGGC CGACGCTGAC GCGCTACGAA CGCTCGCAGA TTCTCGAACG TGCGGCCGCG CTGCTGCGCG AGCGCACCGA GGAGGCGTCC GACCTGATCA CGCTCGAGTC GGGGTTGTCG AAACAGGATT CGCGCTACGA GATCGGCCGG GTGGCCGACG TGCTGAAGTT CGCGGCGGTC GAGGCGCTGC GTGACGATGC GCAGAGTTTC TCTTGCGACC TGACGCCCCA CGGCAAGGCG CGGCGCGTGT TCTCGCAGCG CCAGCCGCTC GACGGCGTGA TCGTCGCGAT TACGCCGTTC AATCATCCGA TGAACCAGGT CGCGCACAAG ATCGCGCCGG CGATCGCGAC CAACAACCGC GTGATCGTGA AGCCGTCGGA GAAGGTGCCG CTGTCGGCGT TCTATCTGGC CGACCTGCTG TATGAAGCCG GGCTGCCGGA ACCGATGCTG CAGGTGTTGA CCGGCGACCC GGCCGAGATC GCGGACGAGC TCGTCACGCA TCCGCATGCG TCGCTGATCA CGTTCACGGG CGGCGTCGCG ATCGGCAAGG CGATTGCGGC GCGGGCCGGC TACCGGCGCA TCGTGCTGGA GCTGGGCGGC AACGATCCGC TGATCGTCCT CGCCGATGCC GATCCCGACC GCGCGGCGTC GCTCGCGGTG TTGGGCTCGT ACCGGAATTC GGGCCAGCGC TGCACGGCCG TCAAGCGGAT GCTGGTGCAG CGCTCCATCG TGCCGGCGTT CACCGAATTG CTCGTCGAGA AGACGCGCGC CTGGAAATAT GGCGATCCAT TCGATCCCTC GAACGAAATG GGCACGGTGA TCGACGAAGC CGCGGCGCGG CTGTTCGAGG CGCGCGTGAA CGAGGCGGTC GCGCAGGGCG CGCGCCTGCT GACGGGCAAT GTGCGGCGCG GCGCGTTGTA CGCGCCGACC GTGCTCGACA ACGTCGATCC GTCGATGACG CTGGTGCGCG AGGAGACCTT CGGGCCCGTG TCGCCCGTCA TCGCATTCGA TACGATCGAC GACGCGATCC GGCTCAGCAA CGGCACGGCG TTCGGGCTGT CGTCGGGTGT CTGTACGGAC AGCACGGCCG CCGTCGTACG GTTCGTGAAC GAGTTGAACG TCGGCACGGT GAACGTATGG GAAGTGCCCG GATACCGGAT CGAACTGTCG CCGTTCGGCG GGATCAAGGA TTCGGGGCTC GGTTACAAGG AAGGCGTCCA GGAGGCGATG AAGAGTTTCA CGAACCTGAA GACGTTTTCG CTGCCTTGGG AGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT