BuceA.00020.r
Putative aldehyde dehydrogenase family protein
| CENTER ID: | BuceA.00020.r |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00020.r.B1.GE36500 | Full length( BuceA.00020.r ) | 1 | 503 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00020.r.B1.PW37259 | Full length( BuceA.00020.r ) | 1 | 503 |
Structures
6DBB
DEPOSITED: 5/2/2018
DETERMINATION: XRay
CLONE: BuceA.00020.r.B1.GE36500
PROTEIN: BuceA.00020.r.B1.PW37259
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130350 |
| PATRIC ID: | fig|216591.5.peg.6277 |
| RefSeq: | YP_002235071.1 |
| UniProt: | B4EIN4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQFNDILAAL DIDLAQWKGN ALTARSPLDG ATLATLAVDT PADAERKIDA AHDAFLKWRT VPAPVRGELV RVFGNVLREH KAELGRLVTL EAGKITSEGL GEVQEMIDIC DFAVGLSRQL YGLTIASERP GHRMMETWHP IGVCGVISAF NFPVAVWAWN AALAFVCGDS VVWKPSEKTP LTAIACHVLL QKALREFEKT HPGVAPAELG QLVLGMRDVG EVLTASKKVP VVSATGSVRM GQEVAKVLSQ RLARGILELG GNNGMIVAPS ADLDLVVRAV TFAAVGTAGQ RCTTLRRLIV HRSLVEQLLP RIEKAYASVK VGNPLEEGTL VGPLVDRASF DAMQKALADA REQGGEVKGG ERVDVGHADA YYVRPAIVRM PKQSAVVERE TFAPILYVMV YDNFDDAIDV HNAVPQGLSS AIFTNDMREA EQFMSAAGSD CGIVNVNIGT SGAEIGGAFG GEKETGGGRE SGSDAWKAYM RRATNTINYS RQLPLAQGVK FDV
NT Sequence
ATGCAATTCA ACGACATTCT TGCCGCGCTC GACATCGATC TCGCCCAGTG GAAAGGCAAC GCGCTGACCG CGCGTTCGCC GCTCGACGGC GCGACGCTCG CGACGCTGGC CGTCGACACG CCCGCCGACG CCGAGCGCAA GATCGACGCC GCGCACGACG CGTTCCTCAA GTGGCGCACG GTGCCGGCGC CGGTGCGCGG CGAACTCGTG CGCGTGTTCG GCAACGTGCT GCGCGAGCAC AAGGCCGAGC TGGGCCGCCT CGTCACGCTC GAAGCCGGCA AGATCACGTC GGAAGGCCTC GGCGAAGTGC AGGAAATGAT CGACATCTGC GATTTCGCGG TCGGCCTGTC GCGCCAGCTT TACGGCCTCA CGATCGCGTC CGAGCGCCCG GGCCACCGGA TGATGGAAAC GTGGCACCCG ATCGGCGTGT GCGGCGTGAT TTCGGCGTTC AACTTCCCGG TCGCGGTGTG GGCGTGGAAT GCGGCGCTCG CGTTCGTGTG CGGCGATTCG GTCGTGTGGA AGCCGTCCGA GAAGACGCCG CTCACCGCGA TCGCGTGCCA CGTGCTGCTC CAGAAGGCGC TGCGCGAGTT CGAGAAGACG CATCCGGGCG TCGCGCCGGC GGAACTGGGT CAACTGGTGC TCGGCATGCG CGACGTCGGC GAGGTGCTGA CGGCGTCGAA GAAGGTGCCG GTCGTCAGCG CGACGGGCAG CGTGCGGATG GGCCAGGAAG TCGCGAAGGT GCTGAGCCAG CGCCTCGCGC GCGGCATCCT CGAACTCGGC GGCAACAACG GGATGATCGT CGCGCCGAGC GCGGATCTCG ACCTCGTCGT GCGCGCGGTC ACGTTCGCGG CGGTCGGCAC GGCCGGCCAG CGCTGCACGA CGCTGCGCCG CCTGATCGTG CATCGCAGCC TCGTCGAGCA ACTGCTGCCG CGCATCGAGA AGGCCTATGC GTCGGTGAAG GTCGGCAATC CGCTCGAGGA AGGCACGCTG GTCGGCCCGC TGGTCGATCG TGCGTCGTTC GACGCGATGC AAAAGGCGCT GGCCGATGCG CGCGAGCAGG GCGGCGAAGT GAAGGGCGGC GAGCGCGTCG ACGTCGGCCA CGCGGATGCG TACTACGTGC GTCCGGCCAT CGTGCGGATG CCGAAGCAAT CGGCGGTGGT CGAGCGCGAG ACGTTCGCGC CGATTCTGTA CGTGATGGTC TACGACAACT TCGACGACGC GATCGACGTG CACAACGCGG TCCCGCAAGG CCTGTCGTCG GCGATCTTCA CGAACGACAT GCGTGAAGCC GAGCAGTTCA TGTCGGCGGC CGGCAGCGAT TGCGGGATCG TCAACGTGAA CATCGGCACG AGCGGCGCGG AAATCGGCGG CGCGTTCGGC GGCGAGAAGG AAACGGGCGG CGGCCGCGAG TCGGGTTCGG ATGCATGGAA GGCGTATATG CGCCGGGCGA CGAACACGAT CAACTACAGC CGCCAGCTGC CGCTGGCGCA GGGGGTGAAG TTCGACGTA
Details for BuceA.00020.r.B1.GE36500
| HARVESTED ON: | 1/9/2013 |
| SEQUENCED ON: | 1/7/2013 |
| EXPECTED MW: | 55kDa |
| OBSERVED MW: | 55kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 76 |
Validated AA Sequence
MIDICDFAVG LSRQLYGLTI ASERPGHRMM ETWHPIGVCG VISAFNFPVA VWAWNAALAF VCGDSVVWKP SEKTPLTAIA CHVLLQKALR EFEKTHPGVA PAELGQLVLG MRDVGEVLTA SKKVPVVSAT GSVRMGQEVA KVLSQRLARG ILELGGNNGM IVAPSADLDL VVRAVTFAAV GTAGQRCTTL RRLIVHRSLV EQLLPRIEKA YASVKVGNPL EEGTLVGPLV DRASFDAMQK ALADAREQGG EVKGGERVDV GHADAYYVRP AIVRMPKQSA VVERETFAPI LYVMVYDNFD DAIDVHNAVP QGLSSAIFTN DMREAEQFMS AAGSDCGIVN VNIGTSGAEI GGAFGGEKET GGGRESGSDA WKAYMRRATN TINYSRQLP
Validated NT Sequence
agcggcagct ggcggctgta gttgatcgtg ttcgtcgccc ggcgcatata cgccttccat gcatccgaac ccgactcgcg gccgccgccc gtttccttct cgccgccgaa cgcgccgccg atttccgcgc cgctcgtgcc gatgttcacg ttgacgatcc cgcaatcgct gccggccgcc gacatgaact gctcggcttc acgcatgtcg ttcgtgaaga tcgccgacga caggccttgc gggaccgcgt tgtgcacgtc gatcgcgtcg tcgaagttgt cgtagaccat cacgtacaga atcggcgcga acgtctcgcg ctcgaccacc gccgattgct tcggcatccg cacgatggcc ggacgcacgt agtacgcatc cgcgtggccg acgtcgacgc gctcgccgcc cttcacttcg ccgccctgct cgcgcgcatc ggccagcgcc ttttgcatcg cgtcgaacga cgcacgatcg accagcgggc cgaccagcgt gccttcctcg agcggattgc cgaccttcac cgacgcatag gccttctcga tgcgcggcag cagttgctcg acgaggctgc gatgcacgat caggcggcgc agcgtcgtgc agcgctggcc ggccgtgccg accgccgcga acgtgaccgc gcgcacgacg aggtcgagat ccgcgctcgg cgcgacgatc atcccgttgt tgccgccgag ttcgaggatg ccgcgcgcga ggcgctggct cagcaccttc gcgacttcct ggcccatccg cacgctgccc gtcgcgctga cgaccggcac cttcttcgac gccgtcagca cctcgccgac gtcgcgcatg ccgagcacca gttgacccag ttccgccggc gcgacgcccg gatgcgtctt ctcgaactcg cgcagcgcct tctggagcag cacgtggcac gcgatcgcgg tgagcggcgt cttctcggac ggcttccaca cgaccgaatc gccgcacacg aacgcgagcg ccgcattcca cgcccacacc gcgaccggga agttgaacgc cgaaatcacg ccgcacacgc cgatcgggtg ccacgtttcc atcatccggt ggcccgggcg ctcggacgcg atcgtgaggc cgtaaagctg gcgcgacagg ccgaccgcga aatcgcagat gtcgatcatt tcctgcactt cgccgaggcc ttccgacgtg atcttgccgg cttcgagcgt gacgaggcgg cccagctcgg ccttgtgctc gcgcagcacg ttgccgaaca cgcgcacgag ttcgccgcgc accggcgccg gcaccgtgcg ccacttgagg aacgcgtcgt gcgcggcgtc gatcttgcgc tcggcgtcgg cgggcgtgtc gacggccagc gtcgcgagcg tcgcgccgtc gagcggcgaa cgcgcggtca gcgcgttgcc tttccactgg gcgagatcga tgtcgagcgc ggcaagaatg tcgttgaat
Expected Protein Sequence
MAHHHHHHMQ FNDILAALDI DLAQWKGNAL TARSPLDGAT LATLAVDTPA DAERKIDAAH DAFLKWRTVP APVRGELVRV FGNVLREHKA ELGRLVTLEA GKITSEGLGE VQEMIDICDF AVGLSRQLYG LTIASERPGH RMMETWHPIG VCGVISAFNF PVAVWAWNAA LAFVCGDSVV WKPSEKTPLT AIACHVLLQK ALREFEKTHP GVAPAELGQL VLGMRDVGEV LTASKKVPVV SATGSVRMGQ EVAKVLSQRL ARGILELGGN NGMIVAPSAD LDLVVRAVTF AAVGTAGQRC TTLRRLIVHR SLVEQLLPRI EKAYASVKVG NPLEEGTLVG PLVDRASFDA MQKALADARE QGGEVKGGER VDVGHADAYY VRPAIVRMPK QSAVVERETF APILYVMVYD NFDDAIDVHN AVPQGLSSAI FTNDMREAEQ FMSAAGSDCG IVNVNIGTSG AEIGGAFGGE KETGGGRESG SDAWKAYMRR ATNTINYSRQ LPLAQGVKFD V
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaat tcaacgacat tcttgccgcg ctcgacatcg atctcgccca gtggaaaggc aacgcgctga ccgcgcgttc gccgctcgac ggcgcgacgc tcgcgacgct ggccgtcgac acgcccgccg acgccgagcg caagatcgac gccgcgcacg acgcgttcct caagtggcgc acggtgccgg cgccggtgcg cggcgaactc gtgcgcgtgt tcggcaacgt gctgcgcgag cacaaggccg agctgggccg cctcgtcacg ctcgaagccg gcaagatcac gtcggaaggc ctcggcgaag tgcaggaaat gatcgacatc tgcgatttcg cggtcggcct gtcgcgccag ctttacggcc tcacgatcgc gtccgagcgc ccgggccacc ggatgatgga aacgtggcac ccgatcggcg tgtgcggcgt gatttcggcg ttcaacttcc cggtcgcggt gtgggcgtgg aatgcggcgc tcgcgttcgt gtgcggcgat tcggtcgtgt ggaagccgtc cgagaagacg ccgctcaccg cgatcgcgtg ccacgtgctg ctccagaagg cgctgcgcga gttcgagaag acgcatccgg gcgtcgcgcc ggcggaactg ggtcaactgg tgctcggcat gcgcgacgtc ggcgaggtgc tgacggcgtc gaagaaggtg ccggtcgtca gcgcgacggg cagcgtgcgg atgggccagg aagtcgcgaa ggtgctgagc cagcgcctcg cgcgcggcat cctcgaactc ggcggcaaca acgggatgat cgtcgcgccg agcgcggatc tcgacctcgt cgtgcgcgcg gtcacgttcg cggcggtcgg cacggccggc cagcgctgca cgacgctgcg ccgcctgatc gtgcatcgca gcctcgtcga gcaactgctg ccgcgcatcg agaaggccta tgcgtcggtg aaggtcggca atccgctcga ggaaggcacg ctggtcggcc cgctggtcga tcgtgcgtcg ttcgacgcga tgcaaaaggc gctggccgat gcgcgcgagc agggcggcga agtgaagggc ggcgagcgcg tcgacgtcgg ccacgcggat gcgtactacg tgcgtccggc catcgtgcgg atgccgaagc aatcggcggt ggtcgagcgc gagacgttcg cgccgattct gtacgtgatg gtctacgaca acttcgacga cgcgatcgac gtgcacaacg cggtcccgca aggcctgtcg tcggcgatct tcacgaacga catgcgtgaa gccgagcagt tcatgtcggc ggccggcagc gattgcggga tcgtcaacgt gaacatcggc acgagcggcg cggaaatcgg cggcgcgttc ggcggcgaga aggaaacggg cggcggccgc gagtcgggtt cggatgcatg gaaggcgtat atgcgccggg cgacgaacac gatcaactac agccgccagc tgccgctggc gcagggggtg aagttcgacg tatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BuceA.00020.r.B1.PW37259
| PURIFICATION DATe: | 3/18/2013 |
| CONCENTRATION: | 50mg/ml |
| OBSERVED MW: | 54kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 13 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 76 |
Protocol Notes
notes unavailable
Validated AA Sequence
MIDICDFAVG LSRQLYGLTI ASERPGHRMM ETWHPIGVCG VISAFNFPVA VWAWNAALAF VCGDSVVWKP SEKTPLTAIA CHVLLQKALR EFEKTHPGVA PAELGQLVLG MRDVGEVLTA SKKVPVVSAT GSVRMGQEVA KVLSQRLARG ILELGGNNGM IVAPSADLDL VVRAVTFAAV GTAGQRCTTL RRLIVHRSLV EQLLPRIEKA YASVKVGNPL EEGTLVGPLV DRASFDAMQK ALADAREQGG EVKGGERVDV GHADAYYVRP AIVRMPKQSA VVERETFAPI LYVMVYDNFD DAIDVHNAVP QGLSSAIFTN DMREAEQFMS AAGSDCGIVN VNIGTSGAEI GGAFGGEKET GGGRESGSDA WKAYMRRATN TINYSRQLP
Validated NT Sequence
agcggcagct ggcggctgta gttgatcgtg ttcgtcgccc ggcgcatata cgccttccat gcatccgaac ccgactcgcg gccgccgccc gtttccttct cgccgccgaa cgcgccgccg atttccgcgc cgctcgtgcc gatgttcacg ttgacgatcc cgcaatcgct gccggccgcc gacatgaact gctcggcttc acgcatgtcg ttcgtgaaga tcgccgacga caggccttgc gggaccgcgt tgtgcacgtc gatcgcgtcg tcgaagttgt cgtagaccat cacgtacaga atcggcgcga acgtctcgcg ctcgaccacc gccgattgct tcggcatccg cacgatggcc ggacgcacgt agtacgcatc cgcgtggccg acgtcgacgc gctcgccgcc cttcacttcg ccgccctgct cgcgcgcatc ggccagcgcc ttttgcatcg cgtcgaacga cgcacgatcg accagcgggc cgaccagcgt gccttcctcg agcggattgc cgaccttcac cgacgcatag gccttctcga tgcgcggcag cagttgctcg acgaggctgc gatgcacgat caggcggcgc agcgtcgtgc agcgctggcc ggccgtgccg accgccgcga acgtgaccgc gcgcacgacg aggtcgagat ccgcgctcgg cgcgacgatc atcccgttgt tgccgccgag ttcgaggatg ccgcgcgcga ggcgctggct cagcaccttc gcgacttcct ggcccatccg cacgctgccc gtcgcgctga cgaccggcac cttcttcgac gccgtcagca cctcgccgac gtcgcgcatg ccgagcacca gttgacccag ttccgccggc gcgacgcccg gatgcgtctt ctcgaactcg cgcagcgcct tctggagcag cacgtggcac gcgatcgcgg tgagcggcgt cttctcggac ggcttccaca cgaccgaatc gccgcacacg aacgcgagcg ccgcattcca cgcccacacc gcgaccggga agttgaacgc cgaaatcacg ccgcacacgc cgatcgggtg ccacgtttcc atcatccggt ggcccgggcg ctcggacgcg atcgtgaggc cgtaaagctg gcgcgacagg ccgaccgcga aatcgcagat gtcgatcatt tcctgcactt cgccgaggcc ttccgacgtg atcttgccgg cttcgagcgt gacgaggcgg cccagctcgg ccttgtgctc gcgcagcacg ttgccgaaca cgcgcacgag ttcgccgcgc accggcgccg gcaccgtgcg ccacttgagg aacgcgtcgt gcgcggcgtc gatcttgcgc tcggcgtcgg cgggcgtgtc gacggccagc gtcgcgagcg tcgcgccgtc gagcggcgaa cgcgcggtca gcgcgttgcc tttccactgg gcgagatcga tgtcgagcgc ggcaagaatg tcgttgaat
Expressed Protein Sequence
MAHHHHHHMQ FNDILAALDI DLAQWKGNAL TARSPLDGAT LATLAVDTPA DAERKIDAAH DAFLKWRTVP APVRGELVRV FGNVLREHKA ELGRLVTLEA GKITSEGLGE VQEMIDICDF AVGLSRQLYG LTIASERPGH RMMETWHPIG VCGVISAFNF PVAVWAWNAA LAFVCGDSVV WKPSEKTPLT AIACHVLLQK ALREFEKTHP GVAPAELGQL VLGMRDVGEV LTASKKVPVV SATGSVRMGQ EVAKVLSQRL ARGILELGGN NGMIVAPSAD LDLVVRAVTF AAVGTAGQRC TTLRRLIVHR SLVEQLLPRI EKAYASVKVG NPLEEGTLVG PLVDRASFDA MQKALADARE QGGEVKGGER VDVGHADAYY VRPAIVRMPK QSAVVERETF APILYVMVYD NFDDAIDVHN AVPQGLSSAI FTNDMREAEQ FMSAAGSDCG IVNVNIGTSG AEIGGAFGGE KETGGGRESG SDAWKAYMRR ATNTINYSRQ LPLAQGVKFD V
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAT TCAACGACAT TCTTGCCGCG CTCGACATCG ATCTCGCCCA GTGGAAAGGC AACGCGCTGA CCGCGCGTTC GCCGCTCGAC GGCGCGACGC TCGCGACGCT GGCCGTCGAC ACGCCCGCCG ACGCCGAGCG CAAGATCGAC GCCGCGCACG ACGCGTTCCT CAAGTGGCGC ACGGTGCCGG CGCCGGTGCG CGGCGAACTC GTGCGCGTGT TCGGCAACGT GCTGCGCGAG CACAAGGCCG AGCTGGGCCG CCTCGTCACG CTCGAAGCCG GCAAGATCAC GTCGGAAGGC CTCGGCGAAG TGCAGGAAAT GATCGACATC TGCGATTTCG CGGTCGGCCT GTCGCGCCAG CTTTACGGCC TCACGATCGC GTCCGAGCGC CCGGGCCACC GGATGATGGA AACGTGGCAC CCGATCGGCG TGTGCGGCGT GATTTCGGCG TTCAACTTCC CGGTCGCGGT GTGGGCGTGG AATGCGGCGC TCGCGTTCGT GTGCGGCGAT TCGGTCGTGT GGAAGCCGTC CGAGAAGACG CCGCTCACCG CGATCGCGTG CCACGTGCTG CTCCAGAAGG CGCTGCGCGA GTTCGAGAAG ACGCATCCGG GCGTCGCGCC GGCGGAACTG GGTCAACTGG TGCTCGGCAT GCGCGACGTC GGCGAGGTGC TGACGGCGTC GAAGAAGGTG CCGGTCGTCA GCGCGACGGG CAGCGTGCGG ATGGGCCAGG AAGTCGCGAA GGTGCTGAGC CAGCGCCTCG CGCGCGGCAT CCTCGAACTC GGCGGCAACA ACGGGATGAT CGTCGCGCCG AGCGCGGATC TCGACCTCGT CGTGCGCGCG GTCACGTTCG CGGCGGTCGG CACGGCCGGC CAGCGCTGCA CGACGCTGCG CCGCCTGATC GTGCATCGCA GCCTCGTCGA GCAACTGCTG CCGCGCATCG AGAAGGCCTA TGCGTCGGTG AAGGTCGGCA ATCCGCTCGA GGAAGGCACG CTGGTCGGCC CGCTGGTCGA TCGTGCGTCG TTCGACGCGA TGCAAAAGGC GCTGGCCGAT GCGCGCGAGC AGGGCGGCGA AGTGAAGGGC GGCGAGCGCG TCGACGTCGG CCACGCGGAT GCGTACTACG TGCGTCCGGC CATCGTGCGG ATGCCGAAGC AATCGGCGGT GGTCGAGCGC GAGACGTTCG CGCCGATTCT GTACGTGATG GTCTACGACA ACTTCGACGA CGCGATCGAC GTGCACAACG CGGTCCCGCA AGGCCTGTCG TCGGCGATCT TCACGAACGA CATGCGTGAA GCCGAGCAGT TCATGTCGGC GGCCGGCAGC GATTGCGGGA TCGTCAACGT GAACATCGGC ACGAGCGGCG CGGAAATCGG CGGCGCGTTC GGCGGCGAGA AGGAAACGGG CGGCGGCCGC GAGTCGGGTT CGGATGCATG GAAGGCGTAT ATGCGCCGGG CGACGAACAC GATCAACTAC AGCCGCCAGC TGCCGCTGGC GCAGGGGGTG AAGTTCGACG TATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT