BuceA.00020.q

5-carboxy-2-hydroxymuconate semialdehyde dehydrogenase

CENTER ID: BuceA.00020.q
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00020.q.B1.GE36499 Full length( BuceA.00020.q ) 1 487
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.5110
RefSeq: YP_002233983.1
UniProt: B4EIE4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIKHWIGGR EVESRETFTT LNPATGEVIT DVASGGETEV DAAVRAAKEA FPKWANTPAK ERAKLMRKLG ELIEKNVPML AALETQDTGL PIAQTSKQLI PRASENFNFF AEVCVQMNGR TYPVDDQMLN YTLYQPVGVC ALVSPWNVPF MTATWKTAPC LALGNTAVLK MSELSPLTAD QLGRLALEAG IPPGVLNVVQ GYGATAGDAL VRHPDVRAVS FTGGTVTGKR IMERAGLKKY SMELGGKSPV LIFDDADFDR ALDASLFTIF SINGERCTAG SRIFVQRTIY DRFVQEFARR ANNLVVGDPA DPGTNLGAMI TRQHWEKVTG YIRIGEQEGA RVVAGGADKP AGLPDHLRNG NFVRPTVFAD VDNRMRIAQE EIFGPVACII PFDDEDDGLR LANDTAYGLA SYIWTQDVGK VHRLARGIEA GMVFVNSQNV RDLRQPFGGV KESGTGREGG EYSFEVFAEI KNVCISMGSH HVPRWGV
NT Sequence
ATGACCATCA AGCACTGGAT CGGCGGCCGC GAAGTGGAGA GCCGCGAAAC CTTCACGACG CTGAACCCGG CGACGGGTGA AGTCATCACC GACGTCGCGT CGGGCGGCGA GACCGAGGTC GACGCGGCCG TGCGCGCCGC GAAGGAAGCG TTCCCGAAAT GGGCGAACAC ACCGGCCAAG GAGCGCGCAA AGCTGATGCG CAAGCTCGGC GAGCTGATCG AGAAGAACGT GCCGATGCTC GCCGCGCTGG AGACGCAGGA CACCGGCCTG CCGATCGCGC AGACGAGCAA GCAGCTGATT CCGCGCGCGT CCGAGAACTT CAACTTCTTC GCGGAAGTGT GCGTGCAGAT GAACGGGCGC ACCTATCCGG TCGACGACCA GATGCTCAAC TACACGCTGT ACCAGCCGGT CGGCGTATGT GCGCTGGTGT CGCCGTGGAA CGTGCCGTTC ATGACGGCCA CGTGGAAGAC GGCGCCGTGC CTCGCGCTCG GCAACACGGC CGTGCTGAAG ATGTCGGAGC TGTCGCCGCT GACGGCCGAC CAGCTCGGCC GGCTCGCACT CGAAGCCGGC ATCCCGCCCG GCGTGCTGAA CGTTGTGCAG GGTTACGGCG CGACGGCCGG CGATGCGCTC GTGCGGCATC CGGACGTGCG CGCGGTGTCG TTTACCGGCG GTACGGTCAC CGGCAAGCGG ATCATGGAAC GCGCGGGCCT CAAGAAGTAT TCGATGGAGC TCGGCGGCAA GTCGCCGGTG CTCATTTTCG ACGACGCCGA TTTCGATCGC GCGCTCGACG CGTCGCTGTT CACGATCTTC TCGATCAACG GCGAACGCTG CACCGCCGGC TCGCGGATCT TCGTGCAACG CACGATCTAC GACCGCTTCG TGCAGGAATT CGCGCGCCGC GCGAACAACC TGGTGGTCGG CGATCCGGCC GATCCGGGCA CGAATCTCGG CGCGATGATC ACGCGCCAGC ACTGGGAAAA GGTGACGGGC TACATCCGCA TCGGCGAGCA GGAAGGCGCG CGCGTGGTTG CCGGCGGCGC GGACAAGCCG GCCGGGCTGC CCGACCATCT GCGCAACGGC AACTTCGTGC GGCCGACCGT GTTCGCCGAC GTCGACAACC GGATGCGCAT CGCGCAGGAG GAGATCTTCG GGCCGGTCGC GTGCATCATC CCGTTCGACG ACGAAGACGA CGGGCTGCGG CTTGCGAACG ACACGGCGTA CGGCCTCGCG TCGTACATCT GGACGCAGGA CGTCGGCAAG GTGCATCGCC TCGCGCGCGG CATCGAGGCC GGGATGGTGT TCGTCAACAG CCAGAACGTG CGCGACCTGC GCCAGCCGTT CGGCGGCGTG AAGGAATCGG GTACCGGGCG CGAGGGCGGC GAGTACAGTT TCGAGGTGTT CGCCGAGATC AAGAACGTGT GCATTTCGAT GGGTTCGCAT CACGTCCCGC GCTGGGGCGT G
Details for BuceA.00020.q.B1.GE36499
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/7/2013
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 21
PERCENT COVERAGE: 86
Validated AA Sequence
MGEHTGQGAR KADAQARRAD REERADARRA GDAGHRPADR ADEQAADSAR VRELQLLRGS VRADERAHLS GRRPDAQLHA VPAGRRMCAG VAVERAVHDG HVEDGAVPRA RQHGRAEDVG AVAADGRPAR PARTRSRHPA RRAERCAGLR RDGRRCARAA SGRARGVVYR RYGHRQADHG TRGPQEVFDG ARRQVAGAHF RRRRFRSRAR RVAVHDLLDQ RRTLHRRLAD LRATHDLRPL RAGIRAPREQ PGGRRSGRSG HESRRDDHAP ALGKGDGLHP HRRAGRRARG CRRRGQAGRA ARPSAQRQLR AADRVRRRRQ PDAHRAGGDL RAGRVHHPVR RRRRRAAACE RHGVRPRVVH LDAGRRQGAS PRARHRGRDG VRQQPERARP APAVRRREGI GYRARGRRVQ FRGVRRDQER VHFDGF
Validated NT Sequence
cgaacccatc gaaatgcaca cgttcttgat ctcggcgaac acctcgaaac tgtactcgcc gccctcgcgc ccggtacccg attccttcac gccgccgaac ggctggcgca ggtcgcgcac gttctggctg ttgacgaaca ccatcccggc ctcgatgccg cgcgcgaggc gatgcacctt gccgacgtcc tgcgtccaga tgtacgacgc gaggccgtac gccgtgtcgt tcgcaagccg cagcccgtcg tcttcgtcgt cgaacgggat gatgcacgcg accggcccga agatctcctc ctgcgcgatg cgcatccggt tgtcgacgtc ggcgaacacg gtcggccgca cgaagttgcc gttgcgcaga tggtcgggca gcccggccgg cttgtccgcg ccgccggcaa ccacgcgcgc gccttcctgc tcgccgatgc ggatgtagcc cgtcaccttt tcccagtgct ggcgcgtgat catcgcgccg agattcgtgc ccggatcggc cggatcgccg accaccaggt tgttcgcgcg gcgcgcgaat tcctgcacga agcggtcgta gatcgtgcgt tgcacgaaga tccgcgagcc ggcggtgcag cgttcgccgt tgatcgagaa gatcgtgaac agcgacgcgt cgagcgcgcg atcgaaatcg gcgtcgtcga aaatgagcac cggcgacttg ccgccgagct ccatcgaata cttcttgagg cccgcgcgtt ccatgatccg cttgccggtg accgtaccgc cggtaaacga caccgcgcgc acgtccggat gccgcacgag cgcatcgccg gccgtcgcgc cgtaaccctg cacaacgttc agcacgccgg gcgggatgcc ggcttcgagt gcgagccggc cgagctggtc ggccgtcagc ggcgacagct ccgacatctt cagcacggcc gtgttgccga gcgcgaggca cggcgccgtc ttccacgtgg ccgtcatgaa cggcacgttc cacggcgaca ccagcgcaca tacgccgacc ggctggtaca gcgtgtagtt gagcatctgg tcgtcgaccg gataggtgcg cccgttcatc tgcacgcaca cttccgcgaa gaagttgaag ttctcggacg cgcgcggaat cagctgcttg ctcgtctgcg cgatcggcag gccggtgtcc tgcgtctcca gcgcggcgag catcggcacg ttcttctcga tcagctcgcc gagcttgcgc atcagctttg cgcgctcctt ggccggtgtg ttcgcccatt tcgggaacgc ttccttcgcg gcgcgcacgg ccgcgtcgac ctcggtctcg ccgcccgacg cgacgtcggt gatgacttca cccgtcgccg ggttcagcgt cgtgaaggtt tcgcggctct ccacttcgcg gccgccgatc cagtgcttga t
Expected Protein Sequence
MAHHHHHHMT IKHWIGGREV ESRETFTTLN PATGEVITDV ASGGETEVDA AVRAAKEAFP KWANTPAKER AKLMRKLGEL IEKNVPMLAA LETQDTGLPI AQTSKQLIPR ASENFNFFAE VCVQMNGRTY PVDDQMLNYT LYQPVGVCAL VSPWNVPFMT ATWKTAPCLA LGNTAVLKMS ELSPLTADQL GRLALEAGIP PGVLNVVQGY GATAGDALVR HPDVRAVSFT GGTVTGKRIM ERAGLKKYSM ELGGKSPVLI FDDADFDRAL DASLFTIFSI NGERCTAGSR IFVQRTIYDR FVQEFARRAN NLVVGDPADP GTNLGAMITR QHWEKVTGYI RIGEQEGARV VAGGADKPAG LPDHLRNGNF VRPTVFADVD NRMRIAQEEI FGPVACIIPF DDEDDGLRLA NDTAYGLASY IWTQDVGKVH RLARGIEAGM VFVNSQNVRD LRQPFGGVKE SGTGREGGEY SFEVFAEIKN VCISMGSHHV PRWGV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacca tcaagcactg gatcggcggc cgcgaagtgg agagccgcga aaccttcacg acgctgaacc cggcgacggg tgaagtcatc accgacgtcg cgtcgggcgg cgagaccgag gtcgacgcgg ccgtgcgcgc cgcgaaggaa gcgttcccga aatgggcgaa cacaccggcc aaggagcgcg caaagctgat gcgcaagctc ggcgagctga tcgagaagaa cgtgccgatg ctcgccgcgc tggagacgca ggacaccggc ctgccgatcg cgcagacgag caagcagctg attccgcgcg cgtccgagaa cttcaacttc ttcgcggaag tgtgcgtgca gatgaacggg cgcacctatc cggtcgacga ccagatgctc aactacacgc tgtaccagcc ggtcggcgta tgtgcgctgg tgtcgccgtg gaacgtgccg ttcatgacgg ccacgtggaa gacggcgccg tgcctcgcgc tcggcaacac ggccgtgctg aagatgtcgg agctgtcgcc gctgacggcc gaccagctcg gccggctcgc actcgaagcc ggcatcccgc ccggcgtgct gaacgttgtg cagggttacg gcgcgacggc cggcgatgcg ctcgtgcggc atccggacgt gcgcgcggtg tcgtttaccg gcggtacggt caccggcaag cggatcatgg aacgcgcggg cctcaagaag tattcgatgg agctcggcgg caagtcgccg gtgctcattt tcgacgacgc cgatttcgat cgcgcgctcg acgcgtcgct gttcacgatc ttctcgatca acggcgaacg ctgcaccgcc ggctcgcgga tcttcgtgca acgcacgatc tacgaccgct tcgtgcagga attcgcgcgc cgcgcgaaca acctggtggt cggcgatccg gccgatccgg gcacgaatct cggcgcgatg atcacgcgcc agcactggga aaaggtgacg ggctacatcc gcatcggcga gcaggaaggc gcgcgcgtgg ttgccggcgg cgcggacaag ccggccgggc tgcccgacca tctgcgcaac ggcaacttcg tgcggccgac cgtgttcgcc gacgtcgaca accggatgcg catcgcgcag gaggagatct tcgggccggt cgcgtgcatc atcccgttcg acgacgaaga cgacgggctg cggcttgcga acgacacggc gtacggcctc gcgtcgtaca tctggacgca ggacgtcggc aaggtgcatc gcctcgcgcg cggcatcgag gccgggatgg tgttcgtcaa cagccagaac gtgcgcgacc tgcgccagcc gttcggcggc gtgaaggaat cgggtaccgg gcgcgagggc ggcgagtaca gtttcgaggt gttcgccgag atcaagaacg tgtgcatttc gatgggttcg catcacgtcc cgcgctgggg cgtgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat