BuceA.00020.p

Betaine aldehyde dehydrogenase

CENTER ID: BuceA.00020.p
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00020.p.B1.GE36498 Full length( BuceA.00020.p ) 1 489
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00020.p.B1.PW37251 Full length( BuceA.00020.p ) 1 489

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.6134
RefSeq: YP_002234941.1
UniProt: B4EHJ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVYGLQRLY IGGGYVDATS GKTFDTFDPA TGELLAQVQQ ASAADVDRAV ASAQEGQREW AAMTAMQRSR ILRRAVDLLR ERNDELAAIE TRDTGKPIGE TLAVDIVTGA DVIEYYAGLA TAIEGLQVPL RAESFVYTRR EPLGVCAGIG AWNYPIQIAC WKTAPALAAG NAMVFKPSEV TPLTALKLAE IYTEAGVPAG VFNVVQGDGS VGALLTGHPD IAKVSFTGGV ETGKKVMSLA GASSLKEVTM ELGGKSPLIV FDDADLDRAA DIAVTANFFS SGQVCTNGTR VFMHRSVKDA FTQKVLERVK RIRVGKPTDA DTNFGPLVSA AQLDKVLGFI ESGKAEGAKL LAGGTRLTDG HFGSGQYVAP TVFGDCRDDM KIVREEIFGP VMSILDFESE DEVIARANDT HYGLAAGVVT ENLSRAHRTI HRLEAGICWI NTWGESPAEM PVGGYKQSGV GRENGITTLE HYTRIKSVQV ELGRYNPVF
NT Sequence
ATGTCCGTAT ACGGTTTGCA GCGCCTCTAC ATCGGCGGCG GTTACGTCGA CGCCACCAGC GGCAAGACTT TCGACACCTT CGATCCCGCC ACCGGCGAAC TGCTCGCACA GGTGCAGCAG GCGAGCGCGG CCGACGTCGA CCGCGCGGTC GCGTCCGCCC AGGAAGGCCA GCGCGAATGG GCCGCGATGA CCGCGATGCA GCGCTCGCGC ATCCTGCGCC GCGCGGTCGA CCTGCTGCGC GAACGCAACG ACGAACTCGC GGCCATCGAA ACGCGCGACA CCGGCAAGCC GATCGGCGAG ACGCTCGCGG TCGACATCGT CACCGGCGCG GACGTGATCG AGTACTACGC GGGCCTCGCG ACCGCGATCG AAGGGCTGCA GGTGCCGCTG CGCGCCGAGT CGTTCGTCTA TACGCGCCGC GAGCCGCTCG GCGTGTGCGC GGGCATCGGC GCGTGGAACT ACCCGATCCA GATCGCCTGC TGGAAGACGG CGCCCGCGCT CGCGGCCGGC AACGCGATGG TGTTCAAGCC GAGCGAAGTC ACGCCGCTGA CCGCGCTGAA GCTCGCGGAA ATCTATACGG AAGCCGGCGT GCCGGCCGGC GTGTTCAACG TCGTGCAGGG CGACGGCTCG GTCGGCGCGC TGCTCACCGG CCATCCGGAC ATCGCGAAGG TGTCGTTCAC CGGCGGCGTC GAAACCGGCA AGAAAGTGAT GTCGCTGGCC GGCGCGTCGT CGCTGAAGGA AGTGACGATG GAGCTCGGCG GCAAGTCGCC GCTGATCGTG TTCGACGATG CGGACCTCGA TCGCGCGGCG GACATCGCGG TGACCGCCAA CTTCTTCAGC TCGGGCCAGG TCTGCACGAA CGGCACGCGC GTGTTCATGC ACCGCTCGGT CAAGGACGCC TTCACGCAGA AGGTGCTCGA ACGCGTGAAA CGCATCCGCG TCGGCAAGCC GACCGATGCC GACACCAACT TCGGCCCGCT CGTGTCGGCC GCGCAGCTCG ACAAGGTGCT CGGCTTCATC GAAAGCGGCA AGGCCGAAGG CGCGAAGCTG CTCGCGGGCG GCACGCGCCT GACCGACGGC CATTTCGGCA GCGGCCAGTA CGTCGCGCCG ACCGTGTTCG GCGATTGCCG CGACGACATG AAGATCGTGC GCGAGGAAAT CTTCGGGCCG GTAATGAGCA TCCTCGATTT CGAATCGGAG GACGAAGTGA TCGCACGTGC GAACGACACG CACTACGGGC TCGCGGCCGG GGTCGTGACC GAGAACCTGT CGCGCGCGCA CCGCACGATC CATCGCCTCG AAGCCGGCAT CTGCTGGATC AACACGTGGG GCGAGTCGCC GGCCGAAATG CCGGTTGGCG GATACAAGCA ATCCGGTGTC GGGCGCGAGA ACGGCATCAC GACGCTCGAG CACTACACTC GCATCAAGTC GGTGCAGGTC GAGCTCGGCC GCTACAACCC GGTGTTT
Details for BuceA.00020.p.B1.GE36498
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/7/2013
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 20
PERCENT COVERAGE: 86
Validated AA Sequence
MDRAVRARQV LGHDPGREPV VRVVRTCDHF VLRFEIEDAH YRPEDFLAHD LHVVAAIAEH GRRDVLAAAE MAVGQARAAR EQLRAFGLAA FDEAEHLVEL RGRHERAEVG VGIGRLADAD AFHAFEHLLR EGVLDRAVHE HARAVRADLA RAEEVGGHRD VRRAIEVRIV EHDQRRLAAE LHRHFLQRRR AGQRHHFLAG FDAAGERHLR DVRMAGEQRA DRAVALHDVE HAGRHAGFRI DFRELQRGQR RDFARLEHHR VAGRERGRRL PAGDLDRVVP RADARAHAER LAARIDERLG AQRHLQPFDR GREARVVLDH VRAGDDVDRE RLADRLAGVA RFDGREFVVA FAQQVDRAAQ DARALHRGHR GPFALAFLGG RDRAVDVGRA RLLHLCEQFA GGGIEGVESL AAGGVDVTAA DVEALQTV
Validated NT Sequence
ctcgacctgc accgacttga tgcgagtgta gtgctcgagc gtcgtgatgc cgttctcgcg cccgacaccg gattgcttgt atccgccaac cggcatttcg gccggcgact cgccccacgt gttgatccag cagatgccgg cttcgaggcg atggatcgtg cggtgcgcgc gcgacaggtt ctcggtcacg accccggccg cgagcccgta gtgcgtgtcg ttcgcacgtg cgatcacttc gtcctccgat tcgaaatcga ggatgctcat taccggcccg aagatttcct cgcgcacgat cttcatgtcg tcgcggcaat cgccgaacac ggtcggcgcg acgtactggc cgctgccgaa atggccgtcg gtcaggcgcg tgccgcccgc gagcagcttc gcgccttcgg ccttgccgct ttcgatgaag ccgagcacct tgtcgagctg cgcggccgac acgagcgggc cgaagttggt gtcggcatcg gtcggcttgc cgacgcggat gcgtttcacg cgttcgagca ccttctgcgt gaaggcgtcc ttgaccgagc ggtgcatgaa cacgcgcgtg ccgttcgtgc agacctggcc cgagctgaag aagttggcgg tcaccgcgat gtccgccgcg cgatcgaggt ccgcatcgtc gaacacgatc agcggcgact tgccgccgag ctccatcgtc acttccttca gcgacgacgc gccggccagc gacatcactt tcttgccggt ttcgacgccg ccggtgaacg acaccttcgc gatgtccgga tggccggtga gcagcgcgcc gaccgagccg tcgccctgca cgacgttgaa cacgccggcc ggcacgccgg cttccgtata gatttccgcg agcttcagcg cggtcagcgg cgtgacttcg ctcggcttga acaccatcgc gttgccggcc gcgagcgcgg gcgccgtctt ccagcaggcg atctggatcg ggtagttcca cgcgccgatg cccgcgcaca cgccgagcgg ctcgcggcgc gtatagacga acgactcggc gcgcagcggc acctgcagcc cttcgatcgc ggtcgcgagg cccgcgtagt actcgatcac gtccgcgccg gtgacgatgt cgaccgcgag cgtctcgccg atcggcttgc cggtgtcgcg cgtttcgatg gccgcgagtt cgtcgttgcg ttcgcgcagc aggtcgaccg cgcggcgcag gatgcgcgag cgctgcatcg cggtcatcgc ggcccattcg cgctggcctt cctgggcgga cgcgaccgcg cggtcgacgt cggccgcgct cgcctgctgc acctgtgcga gcagttcgcc ggtggcggga tcgaaggtgt cgaaagtctt gccgctggtg gcgtcgacgt aaccgccgcc gatgtagagg cgctgcaaac cgtatac
Expected Protein Sequence
MAHHHHHHMS VYGLQRLYIG GGYVDATSGK TFDTFDPATG ELLAQVQQAS AADVDRAVAS AQEGQREWAA MTAMQRSRIL RRAVDLLRER NDELAAIETR DTGKPIGETL AVDIVTGADV IEYYAGLATA IEGLQVPLRA ESFVYTRREP LGVCAGIGAW NYPIQIACWK TAPALAAGNA MVFKPSEVTP LTALKLAEIY TEAGVPAGVF NVVQGDGSVG ALLTGHPDIA KVSFTGGVET GKKVMSLAGA SSLKEVTMEL GGKSPLIVFD DADLDRAADI AVTANFFSSG QVCTNGTRVF MHRSVKDAFT QKVLERVKRI RVGKPTDADT NFGPLVSAAQ LDKVLGFIES GKAEGAKLLA GGTRLTDGHF GSGQYVAPTV FGDCRDDMKI VREEIFGPVM SILDFESEDE VIARANDTHY GLAAGVVTEN LSRAHRTIHR LEAGICWINT WGESPAEMPV GGYKQSGVGR ENGITTLEHY TRIKSVQVEL GRYNPVF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg tatacggttt gcagcgcctc tacatcggcg gcggttacgt cgacgccacc agcggcaaga ctttcgacac cttcgatccc gccaccggcg aactgctcgc acaggtgcag caggcgagcg cggccgacgt cgaccgcgcg gtcgcgtccg cccaggaagg ccagcgcgaa tgggccgcga tgaccgcgat gcagcgctcg cgcatcctgc gccgcgcggt cgacctgctg cgcgaacgca acgacgaact cgcggccatc gaaacgcgcg acaccggcaa gccgatcggc gagacgctcg cggtcgacat cgtcaccggc gcggacgtga tcgagtacta cgcgggcctc gcgaccgcga tcgaagggct gcaggtgccg ctgcgcgccg agtcgttcgt ctatacgcgc cgcgagccgc tcggcgtgtg cgcgggcatc ggcgcgtgga actacccgat ccagatcgcc tgctggaaga cggcgcccgc gctcgcggcc ggcaacgcga tggtgttcaa gccgagcgaa gtcacgccgc tgaccgcgct gaagctcgcg gaaatctata cggaagccgg cgtgccggcc ggcgtgttca acgtcgtgca gggcgacggc tcggtcggcg cgctgctcac cggccatccg gacatcgcga aggtgtcgtt caccggcggc gtcgaaaccg gcaagaaagt gatgtcgctg gccggcgcgt cgtcgctgaa ggaagtgacg atggagctcg gcggcaagtc gccgctgatc gtgttcgacg atgcggacct cgatcgcgcg gcggacatcg cggtgaccgc caacttcttc agctcgggcc aggtctgcac gaacggcacg cgcgtgttca tgcaccgctc ggtcaaggac gccttcacgc agaaggtgct cgaacgcgtg aaacgcatcc gcgtcggcaa gccgaccgat gccgacacca acttcggccc gctcgtgtcg gccgcgcagc tcgacaaggt gctcggcttc atcgaaagcg gcaaggccga aggcgcgaag ctgctcgcgg gcggcacgcg cctgaccgac ggccatttcg gcagcggcca gtacgtcgcg ccgaccgtgt tcggcgattg ccgcgacgac atgaagatcg tgcgcgagga aatcttcggg ccggtaatga gcatcctcga tttcgaatcg gaggacgaag tgatcgcacg tgcgaacgac acgcactacg ggctcgcggc cggggtcgtg accgagaacc tgtcgcgcgc gcaccgcacg atccatcgcc tcgaagccgg catctgctgg atcaacacgt ggggcgagtc gccggccgaa atgccggttg gcggatacaa gcaatccggt gtcgggcgcg agaacggcat cacgacgctc gagcactaca ctcgcatcaa gtcggtgcag gtcgagctcg gccgctacaa cccggtgttt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuceA.00020.p.B1.PW37251
PURIFICATION DATe: 3/4/2013
CONCENTRATION: 33.1mg/ml
OBSERVED MW: 54kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 18
VIAL VOLUME: 200µl
PERCENT IDENTITY: 20
PERCENT COVERAGE: 86
Protocol Notes
notes unavailable
Validated AA Sequence
MDRAVRARQV LGHDPGREPV VRVVRTCDHF VLRFEIEDAH YRPEDFLAHD LHVVAAIAEH GRRDVLAAAE MAVGQARAAR EQLRAFGLAA FDEAEHLVEL RGRHERAEVG VGIGRLADAD AFHAFEHLLR EGVLDRAVHE HARAVRADLA RAEEVGGHRD VRRAIEVRIV EHDQRRLAAE LHRHFLQRRR AGQRHHFLAG FDAAGERHLR DVRMAGEQRA DRAVALHDVE HAGRHAGFRI DFRELQRGQR RDFARLEHHR VAGRERGRRL PAGDLDRVVP RADARAHAER LAARIDERLG AQRHLQPFDR GREARVVLDH VRAGDDVDRE RLADRLAGVA RFDGREFVVA FAQQVDRAAQ DARALHRGHR GPFALAFLGG RDRAVDVGRA RLLHLCEQFA GGGIEGVESL AAGGVDVTAA DVEALQTV
Validated NT Sequence
ctcgacctgc accgacttga tgcgagtgta gtgctcgagc gtcgtgatgc cgttctcgcg cccgacaccg gattgcttgt atccgccaac cggcatttcg gccggcgact cgccccacgt gttgatccag cagatgccgg cttcgaggcg atggatcgtg cggtgcgcgc gcgacaggtt ctcggtcacg accccggccg cgagcccgta gtgcgtgtcg ttcgcacgtg cgatcacttc gtcctccgat tcgaaatcga ggatgctcat taccggcccg aagatttcct cgcgcacgat cttcatgtcg tcgcggcaat cgccgaacac ggtcggcgcg acgtactggc cgctgccgaa atggccgtcg gtcaggcgcg tgccgcccgc gagcagcttc gcgccttcgg ccttgccgct ttcgatgaag ccgagcacct tgtcgagctg cgcggccgac acgagcgggc cgaagttggt gtcggcatcg gtcggcttgc cgacgcggat gcgtttcacg cgttcgagca ccttctgcgt gaaggcgtcc ttgaccgagc ggtgcatgaa cacgcgcgtg ccgttcgtgc agacctggcc cgagctgaag aagttggcgg tcaccgcgat gtccgccgcg cgatcgaggt ccgcatcgtc gaacacgatc agcggcgact tgccgccgag ctccatcgtc acttccttca gcgacgacgc gccggccagc gacatcactt tcttgccggt ttcgacgccg ccggtgaacg acaccttcgc gatgtccgga tggccggtga gcagcgcgcc gaccgagccg tcgccctgca cgacgttgaa cacgccggcc ggcacgccgg cttccgtata gatttccgcg agcttcagcg cggtcagcgg cgtgacttcg ctcggcttga acaccatcgc gttgccggcc gcgagcgcgg gcgccgtctt ccagcaggcg atctggatcg ggtagttcca cgcgccgatg cccgcgcaca cgccgagcgg ctcgcggcgc gtatagacga acgactcggc gcgcagcggc acctgcagcc cttcgatcgc ggtcgcgagg cccgcgtagt actcgatcac gtccgcgccg gtgacgatgt cgaccgcgag cgtctcgccg atcggcttgc cggtgtcgcg cgtttcgatg gccgcgagtt cgtcgttgcg ttcgcgcagc aggtcgaccg cgcggcgcag gatgcgcgag cgctgcatcg cggtcatcgc ggcccattcg cgctggcctt cctgggcgga cgcgaccgcg cggtcgacgt cggccgcgct cgcctgctgc acctgtgcga gcagttcgcc ggtggcggga tcgaaggtgt cgaaagtctt gccgctggtg gcgtcgacgt aaccgccgcc gatgtagagg cgctgcaaac cgtatac
Expressed Protein Sequence
MAHHHHHHMS VYGLQRLYIG GGYVDATSGK TFDTFDPATG ELLAQVQQAS AADVDRAVAS AQEGQREWAA MTAMQRSRIL RRAVDLLRER NDELAAIETR DTGKPIGETL AVDIVTGADV IEYYAGLATA IEGLQVPLRA ESFVYTRREP LGVCAGIGAW NYPIQIACWK TAPALAAGNA MVFKPSEVTP LTALKLAEIY TEAGVPAGVF NVVQGDGSVG ALLTGHPDIA KVSFTGGVET GKKVMSLAGA SSLKEVTMEL GGKSPLIVFD DADLDRAADI AVTANFFSSG QVCTNGTRVF MHRSVKDAFT QKVLERVKRI RVGKPTDADT NFGPLVSAAQ LDKVLGFIES GKAEGAKLLA GGTRLTDGHF GSGQYVAPTV FGDCRDDMKI VREEIFGPVM SILDFESEDE VIARANDTHY GLAAGVVTEN LSRAHRTIHR LEAGICWINT WGESPAEMPV GGYKQSGVGR ENGITTLEHY TRIKSVQVEL GRYNPVF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG TATACGGTTT GCAGCGCCTC TACATCGGCG GCGGTTACGT CGACGCCACC AGCGGCAAGA CTTTCGACAC CTTCGATCCC GCCACCGGCG AACTGCTCGC ACAGGTGCAG CAGGCGAGCG CGGCCGACGT CGACCGCGCG GTCGCGTCCG CCCAGGAAGG CCAGCGCGAA TGGGCCGCGA TGACCGCGAT GCAGCGCTCG CGCATCCTGC GCCGCGCGGT CGACCTGCTG CGCGAACGCA ACGACGAACT CGCGGCCATC GAAACGCGCG ACACCGGCAA GCCGATCGGC GAGACGCTCG CGGTCGACAT CGTCACCGGC GCGGACGTGA TCGAGTACTA CGCGGGCCTC GCGACCGCGA TCGAAGGGCT GCAGGTGCCG CTGCGCGCCG AGTCGTTCGT CTATACGCGC CGCGAGCCGC TCGGCGTGTG CGCGGGCATC GGCGCGTGGA ACTACCCGAT CCAGATCGCC TGCTGGAAGA CGGCGCCCGC GCTCGCGGCC GGCAACGCGA TGGTGTTCAA GCCGAGCGAA GTCACGCCGC TGACCGCGCT GAAGCTCGCG GAAATCTATA CGGAAGCCGG CGTGCCGGCC GGCGTGTTCA ACGTCGTGCA GGGCGACGGC TCGGTCGGCG CGCTGCTCAC CGGCCATCCG GACATCGCGA AGGTGTCGTT CACCGGCGGC GTCGAAACCG GCAAGAAAGT GATGTCGCTG GCCGGCGCGT CGTCGCTGAA GGAAGTGACG ATGGAGCTCG GCGGCAAGTC GCCGCTGATC GTGTTCGACG ATGCGGACCT CGATCGCGCG GCGGACATCG CGGTGACCGC CAACTTCTTC AGCTCGGGCC AGGTCTGCAC GAACGGCACG CGCGTGTTCA TGCACCGCTC GGTCAAGGAC GCCTTCACGC AGAAGGTGCT CGAACGCGTG AAACGCATCC GCGTCGGCAA GCCGACCGAT GCCGACACCA ACTTCGGCCC GCTCGTGTCG GCCGCGCAGC TCGACAAGGT GCTCGGCTTC ATCGAAAGCG GCAAGGCCGA AGGCGCGAAG CTGCTCGCGG GCGGCACGCG CCTGACCGAC GGCCATTTCG GCAGCGGCCA GTACGTCGCG CCGACCGTGT TCGGCGATTG CCGCGACGAC ATGAAGATCG TGCGCGAGGA AATCTTCGGG CCGGTAATGA GCATCCTCGA TTTCGAATCG GAGGACGAAG TGATCGCACG TGCGAACGAC ACGCACTACG GGCTCGCGGC CGGGGTCGTG ACCGAGAACC TGTCGCGCGC GCACCGCACG ATCCATCGCC TCGAAGCCGG CATCTGCTGG ATCAACACGT GGGGCGAGTC GCCGGCCGAA ATGCCGGTTG GCGGATACAA GCAATCCGGT GTCGGGCGCG AGAACGGCAT CACGACGCTC GAGCACTACA CTCGCATCAA GTCGGTGCAG GTCGAGCTCG GCCGCTACAA CCCGGTGTTT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT