BuceA.00020.o
Putative phenylacetaldehyde dehydrogenase
| CENTER ID: | BuceA.00020.o |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00020.o.B1.GE36497 | Full length( BuceA.00020.o ) | 1 | 503 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00020.o.B1.PS01777 | Full length( BuceA.00020.o ) | 1 | 503 |
Structures
4O5H
DEPOSITED: 12/19/2013
DETERMINATION: XRay
CLONE: BuceA.00020.o.B1.GE36497
PROTEIN: BuceA.00020.o.B1.PS01777
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|216591.5.peg.6088 |
| RefSeq: | YP_002234897.1 |
| UniProt: | B4EH14 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNMETNETFA LLDATRAFLA KPKQMLIGAE WSDAASGRQL DVVNPADGTV IARVPEADER DVQQAVAAAR RAFDAGPWRT AKTTDRERLM LVLADLIEAN ARELAEIESL DNGKPVMVAQ GLDVAMAAQC FRYMAGWATK IEGSVIDAGM PYLPDSEIFA YTRKEPVGVV GAIIPWNFPL LMAAWKIAPA LATGCTVVLK PAEDTPLSAL RLGELIQAAG FPDGVVNIVT GYGHTAGAAL SRDPRIDKIA FTGSTQTGKT IGHAALDNMT RMSLELGGKS PVIVLPDVDL DKAAQGVANA IFFNQGQVCT AGSRAYIHSK VFDGVIERVA KIAASLKIGP GMDPATQIGP LVSAKQRERV CGYIDSGFGE GARAAAGGRA IDGPGFFVEP TVLVDTTQAM RVVREEIFGP VLVAMPFDDV DTAVQLANDT PYGLGASIWS NDLSAIHKLV PRIAAGTVWV NCHSLLDNAL PFGGMKQSGF GRELGRAVID QYTESKSVMM NYA
NT Sequence
GTGAACATGG AGACGAACGA AACCTTTGCG CTGCTCGACG CGACGCGCGC GTTTCTCGCG AAGCCGAAGC AGATGCTGAT CGGCGCCGAA TGGAGCGATG CCGCGTCGGG CCGCCAGCTC GATGTGGTGA ACCCGGCCGA CGGCACCGTG ATTGCGCGCG TGCCCGAAGC GGACGAGCGC GACGTGCAGC AGGCGGTCGC CGCCGCGCGC CGCGCCTTCG ATGCGGGCCC GTGGCGCACC GCGAAGACCA CCGACCGCGA GCGCCTGATG CTCGTGCTGG CCGACCTGAT CGAAGCCAAC GCGCGCGAGC TGGCCGAGAT CGAATCGCTC GACAACGGCA AGCCGGTGAT GGTCGCGCAG GGGCTCGACG TCGCGATGGC CGCGCAGTGC TTCCGTTACA TGGCCGGCTG GGCGACGAAG ATCGAAGGCA GCGTGATCGA TGCCGGCATG CCGTACCTGC CGGACAGCGA GATCTTCGCC TATACGAGAA AGGAACCCGT GGGCGTGGTC GGCGCGATCA TCCCGTGGAA TTTCCCGCTG CTGATGGCCG CGTGGAAAAT CGCGCCGGCG CTCGCGACCG GCTGCACCGT CGTGCTGAAG CCTGCCGAGG ATACGCCGTT GAGCGCGCTG CGGCTGGGCG AGCTGATCCA GGCCGCCGGG TTTCCGGACG GTGTCGTCAA TATCGTCACC GGCTACGGTC ACACGGCGGG CGCCGCGTTG TCGCGCGATC CGCGCATCGA CAAGATCGCG TTCACCGGAT CGACGCAGAC GGGCAAGACG ATCGGCCACG CGGCGCTCGA CAACATGACG CGGATGTCGC TGGAACTCGG CGGCAAGTCA CCGGTGATCG TGCTGCCCGA CGTCGATCTC GACAAGGCCG CCCAGGGCGT CGCGAACGCG ATCTTCTTCA ACCAGGGGCA GGTGTGCACG GCCGGCTCGC GCGCCTACAT CCATTCGAAG GTGTTCGACG GCGTGATCGA GCGCGTCGCG AAGATCGCCG CGAGCCTGAA GATCGGCCCG GGGATGGACC CGGCAACGCA GATCGGCCCG CTCGTCTCGG CGAAGCAGCG CGAGCGCGTG TGCGGTTACA TCGACTCGGG GTTCGGCGAG GGCGCGCGTG CCGCTGCGGG CGGCCGCGCG ATCGACGGCC CCGGCTTCTT CGTCGAACCG ACGGTGCTGG TGGATACGAC GCAGGCGATG CGCGTGGTGC GCGAGGAGAT CTTCGGGCCG GTGCTCGTCG CGATGCCGTT CGACGACGTC GATACGGCCG TGCAGCTCGC GAACGACACG CCGTACGGCC TCGGCGCGAG CATCTGGTCG AACGATCTGT CCGCGATCCA CAAGCTGGTC CCGCGTATTG CCGCGGGCAC CGTGTGGGTC AACTGCCACT CGCTGCTCGA CAACGCGCTG CCGTTCGGCG GGATGAAGCA GTCGGGCTTC GGCCGCGAAC TCGGTCGCGC GGTGATCGAC CAGTACACGG AAAGCAAGTC GGTGATGATG AACTACGCG
Details for BuceA.00020.o.B1.GE36497
| HARVESTED ON: | 1/9/2013 |
| SEQUENCED ON: | 1/7/2013 |
| EXPECTED MW: | 55kDa |
| OBSERVED MW: | 54kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
METNETFALL DATRAFLAKP KQMLIGAEWS DAASGRQLDV VNPADGTVIA RVPEADERDV QQAVAAARRA FDAGPWRTAK TTDRERLMLV LADLIEANAR ELAEIESLDN GKPVMVAQGL DVAMAAQCFR YMAGWATKIE GSVIDAGMPY LPDSEIFAYT RKEPVGVVGA IIPWNFPLLM AAWKIAPALA TGCTVVLKPA EDTPLSALRL GELIQAAGFP DGVVNIVTGY GHTAGAALSR DPRIDKIAFT GSTQTGKTIG HAALDNMTRM SLELGGKSPV IVLPDVDLDK AAQGVANAIF FNQGQVCTAG SRAYIHSKVF DGVIERVAKI AASLKIGPGM DPATQIGPLV SAKQRERVCG YIDSGFGEGA RAAAGGRAID GPGFFVEPTV LVDTTQAMRV VREEIFGPVL VAMPFDDVDT AVQLANDTPY GLGASIWSND LSAIHKLVPR IAAGTVWVNC HSLLDNALPF GGMKQSGFGR ELGRAVIDQY TE
Validated NT Sequence
ctttccgtgt actggtcgat caccgcgcga ccgagttcgc ggccgaagcc cgactgcttc atcccgccga acggcagcgc gttgtcgagc agcgagtggc agttgaccca cacggtgccc gcggcaatac gcgggaccag cttgtggatc gcggacagat cgttcgacca gatgctcgcg ccgaggccgt acggcgtgtc gttcgcgagc tgcacggccg tatcgacgtc gtcgaacggc atcgcgacga gcaccggccc gaagatctcc tcgcgcacca cgcgcatcgc ctgcgtcgta tccaccagca ccgtcggttc gacgaagaag ccggggccgt cgatcgcgcg gccgcccgca gcggcacgcg cgccctcgcc gaaccccgag tcgatgtaac cgcacacgcg ctcgcgctgc ttcgccgaga cgagcgggcc gatctgcgtt gccgggtcca tccccgggcc gatcttcagg ctcgcggcga tcttcgcgac gcgctcgatc acgccgtcga acaccttcga atggatgtag gcgcgcgagc cggccgtgca cacctgcccc tggttgaaga agatcgcgtt cgcgacgccc tgggcggcct tgtcgagatc gacgtcgggc agcacgatca ccggtgactt gccgccgagt tccagcgaca tccgcgtcat gttgtcgagc gccgcgtggc cgatcgtctt gcccgtctgc gtcgatccgg tgaacgcgat cttgtcgatg cgcggatcgc gcgacaacgc ggcgcccgcc gtgtgaccgt agccggtgac gatattgacg acaccgtccg gaaacccggc ggcctggatc agctcgccca gccgcagcgc gctcaacggc gtatcctcgg caggcttcag cacgacggtg cagccggtcg cgagcgccgg cgcgattttc cacgcggcca tcagcagcgg gaaattccac gggatgatcg cgccgaccac gcccacgggt tcctttctcg tataggcgaa gatctcgctg tccggcaggt acggcatgcc ggcatcgatc acgctgcctt cgatcttcgt cgcccagccg gccatgtaac ggaagcactg cgcggccatc gcgacgtcga gcccctgcgc gaccatcacc ggcttgccgt tgtcgagcga ttcgatctcg gccagctcgc gcgcgttggc ttcgatcagg tcggccagca cgagcatcag gcgctcgcgg tcggtggtct tcgcggtgcg ccacgggccc gcatcgaagg cgcggcgcgc ggcggcgacc gcctgctgca cgtcgcgctc gtccgcttcg ggcacgcgcg caatcacggt gccgtcggcc gggttcacca catcgagctg gcggcccgac gcggcatcgc tccattcggc gccgatcagc atctgcttcg gcttcgcgag aaacgcgcgc gtcgcgtcga gcagcgcaaa ggtttcgttc gtctccatgt tc
Expected Protein Sequence
MAHHHHHHMV NMETNETFAL LDATRAFLAK PKQMLIGAEW SDAASGRQLD VVNPADGTVI ARVPEADERD VQQAVAAARR AFDAGPWRTA KTTDRERLML VLADLIEANA RELAEIESLD NGKPVMVAQG LDVAMAAQCF RYMAGWATKI EGSVIDAGMP YLPDSEIFAY TRKEPVGVVG AIIPWNFPLL MAAWKIAPAL ATGCTVVLKP AEDTPLSALR LGELIQAAGF PDGVVNIVTG YGHTAGAALS RDPRIDKIAF TGSTQTGKTI GHAALDNMTR MSLELGGKSP VIVLPDVDLD KAAQGVANAI FFNQGQVCTA GSRAYIHSKV FDGVIERVAK IAASLKIGPG MDPATQIGPL VSAKQRERVC GYIDSGFGEG ARAAAGGRAI DGPGFFVEPT VLVDTTQAMR VVREEIFGPV LVAMPFDDVD TAVQLANDTP YGLGASIWSN DLSAIHKLVP RIAAGTVWVN CHSLLDNALP FGGMKQSGFG RELGRAVIDQ YTESKSVMMN YA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga acatggagac gaacgaaacc tttgcgctgc tcgacgcgac gcgcgcgttt ctcgcgaagc cgaagcagat gctgatcggc gccgaatgga gcgatgccgc gtcgggccgc cagctcgatg tggtgaaccc ggccgacggc accgtgattg cgcgcgtgcc cgaagcggac gagcgcgacg tgcagcaggc ggtcgccgcc gcgcgccgcg ccttcgatgc gggcccgtgg cgcaccgcga agaccaccga ccgcgagcgc ctgatgctcg tgctggccga cctgatcgaa gccaacgcgc gcgagctggc cgagatcgaa tcgctcgaca acggcaagcc ggtgatggtc gcgcaggggc tcgacgtcgc gatggccgcg cagtgcttcc gttacatggc cggctgggcg acgaagatcg aaggcagcgt gatcgatgcc ggcatgccgt acctgccgga cagcgagatc ttcgcctata cgagaaagga acccgtgggc gtggtcggcg cgatcatccc gtggaatttc ccgctgctga tggccgcgtg gaaaatcgcg ccggcgctcg cgaccggctg caccgtcgtg ctgaagcctg ccgaggatac gccgttgagc gcgctgcggc tgggcgagct gatccaggcc gccgggtttc cggacggtgt cgtcaatatc gtcaccggct acggtcacac ggcgggcgcc gcgttgtcgc gcgatccgcg catcgacaag atcgcgttca ccggatcgac gcagacgggc aagacgatcg gccacgcggc gctcgacaac atgacgcgga tgtcgctgga actcggcggc aagtcaccgg tgatcgtgct gcccgacgtc gatctcgaca aggccgccca gggcgtcgcg aacgcgatct tcttcaacca ggggcaggtg tgcacggccg gctcgcgcgc ctacatccat tcgaaggtgt tcgacggcgt gatcgagcgc gtcgcgaaga tcgccgcgag cctgaagatc ggcccgggga tggacccggc aacgcagatc ggcccgctcg tctcggcgaa gcagcgcgag cgcgtgtgcg gttacatcga ctcggggttc ggcgagggcg cgcgtgccgc tgcgggcggc cgcgcgatcg acggccccgg cttcttcgtc gaaccgacgg tgctggtgga tacgacgcag gcgatgcgcg tggtgcgcga ggagatcttc gggccggtgc tcgtcgcgat gccgttcgac gacgtcgata cggccgtgca gctcgcgaac gacacgccgt acggcctcgg cgcgagcatc tggtcgaacg atctgtccgc gatccacaag ctggtcccgc gtattgccgc gggcaccgtg tgggtcaact gccactcgct gctcgacaac gcgctgccgt tcggcgggat gaagcagtcg ggcttcggcc gcgaactcgg tcgcgcggtg atcgaccagt acacggaaag caagtcggtg atgatgaact acgcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BuceA.00020.o.B1.PS01777
| PURIFICATION DATe: | 3/27/2013 |
| CONCENTRATION: | 37mg/ml |
| OBSERVED MW: | 53kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
METNETFALL DATRAFLAKP KQMLIGAEWS DAASGRQLDV VNPADGTVIA RVPEADERDV QQAVAAARRA FDAGPWRTAK TTDRERLMLV LADLIEANAR ELAEIESLDN GKPVMVAQGL DVAMAAQCFR YMAGWATKIE GSVIDAGMPY LPDSEIFAYT RKEPVGVVGA IIPWNFPLLM AAWKIAPALA TGCTVVLKPA EDTPLSALRL GELIQAAGFP DGVVNIVTGY GHTAGAALSR DPRIDKIAFT GSTQTGKTIG HAALDNMTRM SLELGGKSPV IVLPDVDLDK AAQGVANAIF FNQGQVCTAG SRAYIHSKVF DGVIERVAKI AASLKIGPGM DPATQIGPLV SAKQRERVCG YIDSGFGEGA RAAAGGRAID GPGFFVEPTV LVDTTQAMRV VREEIFGPVL VAMPFDDVDT AVQLANDTPY GLGASIWSND LSAIHKLVPR IAAGTVWVNC HSLLDNALPF GGMKQSGFGR ELGRAVIDQY TE
Validated NT Sequence
ctttccgtgt actggtcgat caccgcgcga ccgagttcgc ggccgaagcc cgactgcttc atcccgccga acggcagcgc gttgtcgagc agcgagtggc agttgaccca cacggtgccc gcggcaatac gcgggaccag cttgtggatc gcggacagat cgttcgacca gatgctcgcg ccgaggccgt acggcgtgtc gttcgcgagc tgcacggccg tatcgacgtc gtcgaacggc atcgcgacga gcaccggccc gaagatctcc tcgcgcacca cgcgcatcgc ctgcgtcgta tccaccagca ccgtcggttc gacgaagaag ccggggccgt cgatcgcgcg gccgcccgca gcggcacgcg cgccctcgcc gaaccccgag tcgatgtaac cgcacacgcg ctcgcgctgc ttcgccgaga cgagcgggcc gatctgcgtt gccgggtcca tccccgggcc gatcttcagg ctcgcggcga tcttcgcgac gcgctcgatc acgccgtcga acaccttcga atggatgtag gcgcgcgagc cggccgtgca cacctgcccc tggttgaaga agatcgcgtt cgcgacgccc tgggcggcct tgtcgagatc gacgtcgggc agcacgatca ccggtgactt gccgccgagt tccagcgaca tccgcgtcat gttgtcgagc gccgcgtggc cgatcgtctt gcccgtctgc gtcgatccgg tgaacgcgat cttgtcgatg cgcggatcgc gcgacaacgc ggcgcccgcc gtgtgaccgt agccggtgac gatattgacg acaccgtccg gaaacccggc ggcctggatc agctcgccca gccgcagcgc gctcaacggc gtatcctcgg caggcttcag cacgacggtg cagccggtcg cgagcgccgg cgcgattttc cacgcggcca tcagcagcgg gaaattccac gggatgatcg cgccgaccac gcccacgggt tcctttctcg tataggcgaa gatctcgctg tccggcaggt acggcatgcc ggcatcgatc acgctgcctt cgatcttcgt cgcccagccg gccatgtaac ggaagcactg cgcggccatc gcgacgtcga gcccctgcgc gaccatcacc ggcttgccgt tgtcgagcga ttcgatctcg gccagctcgc gcgcgttggc ttcgatcagg tcggccagca cgagcatcag gcgctcgcgg tcggtggtct tcgcggtgcg ccacgggccc gcatcgaagg cgcggcgcgc ggcggcgacc gcctgctgca cgtcgcgctc gtccgcttcg ggcacgcgcg caatcacggt gccgtcggcc gggttcacca catcgagctg gcggcccgac gcggcatcgc tccattcggc gccgatcagc atctgcttcg gcttcgcgag aaacgcgcgc gtcgcgtcga gcagcgcaaa ggtttcgttc gtctccatgt tc
Expressed Protein Sequence
MAHHHHHHMV NMETNETFAL LDATRAFLAK PKQMLIGAEW SDAASGRQLD VVNPADGTVI ARVPEADERD VQQAVAAARR AFDAGPWRTA KTTDRERLML VLADLIEANA RELAEIESLD NGKPVMVAQG LDVAMAAQCF RYMAGWATKI EGSVIDAGMP YLPDSEIFAY TRKEPVGVVG AIIPWNFPLL MAAWKIAPAL ATGCTVVLKP AEDTPLSALR LGELIQAAGF PDGVVNIVTG YGHTAGAALS RDPRIDKIAF TGSTQTGKTI GHAALDNMTR MSLELGGKSP VIVLPDVDLD KAAQGVANAI FFNQGQVCTA GSRAYIHSKV FDGVIERVAK IAASLKIGPG MDPATQIGPL VSAKQRERVC GYIDSGFGEG ARAAAGGRAI DGPGFFVEPT VLVDTTQAMR VVREEIFGPV LVAMPFDDVD TAVQLANDTP YGLGASIWSN DLSAIHKLVP RIAAGTVWVN CHSLLDNALP FGGMKQSGFG RELGRAVIDQ YTESKSVMMN YA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTGA ACATGGAGAC GAACGAAACC TTTGCGCTGC TCGACGCGAC GCGCGCGTTT CTCGCGAAGC CGAAGCAGAT GCTGATCGGC GCCGAATGGA GCGATGCCGC GTCGGGCCGC CAGCTCGATG TGGTGAACCC GGCCGACGGC ACCGTGATTG CGCGCGTGCC CGAAGCGGAC GAGCGCGACG TGCAGCAGGC GGTCGCCGCC GCGCGCCGCG CCTTCGATGC GGGCCCGTGG CGCACCGCGA AGACCACCGA CCGCGAGCGC CTGATGCTCG TGCTGGCCGA CCTGATCGAA GCCAACGCGC GCGAGCTGGC CGAGATCGAA TCGCTCGACA ACGGCAAGCC GGTGATGGTC GCGCAGGGGC TCGACGTCGC GATGGCCGCG CAGTGCTTCC GTTACATGGC CGGCTGGGCG ACGAAGATCG AAGGCAGCGT GATCGATGCC GGCATGCCGT ACCTGCCGGA CAGCGAGATC TTCGCCTATA CGAGAAAGGA ACCCGTGGGC GTGGTCGGCG CGATCATCCC GTGGAATTTC CCGCTGCTGA TGGCCGCGTG GAAAATCGCG CCGGCGCTCG CGACCGGCTG CACCGTCGTG CTGAAGCCTG CCGAGGATAC GCCGTTGAGC GCGCTGCGGC TGGGCGAGCT GATCCAGGCC GCCGGGTTTC CGGACGGTGT CGTCAATATC GTCACCGGCT ACGGTCACAC GGCGGGCGCC GCGTTGTCGC GCGATCCGCG CATCGACAAG ATCGCGTTCA CCGGATCGAC GCAGACGGGC AAGACGATCG GCCACGCGGC GCTCGACAAC ATGACGCGGA TGTCGCTGGA ACTCGGCGGC AAGTCACCGG TGATCGTGCT GCCCGACGTC GATCTCGACA AGGCCGCCCA GGGCGTCGCG AACGCGATCT TCTTCAACCA GGGGCAGGTG TGCACGGCCG GCTCGCGCGC CTACATCCAT TCGAAGGTGT TCGACGGCGT GATCGAGCGC GTCGCGAAGA TCGCCGCGAG CCTGAAGATC GGCCCGGGGA TGGACCCGGC AACGCAGATC GGCCCGCTCG TCTCGGCGAA GCAGCGCGAG CGCGTGTGCG GTTACATCGA CTCGGGGTTC GGCGAGGGCG CGCGTGCCGC TGCGGGCGGC CGCGCGATCG ACGGCCCCGG CTTCTTCGTC GAACCGACGG TGCTGGTGGA TACGACGCAG GCGATGCGCG TGGTGCGCGA GGAGATCTTC GGGCCGGTGC TCGTCGCGAT GCCGTTCGAC GACGTCGATA CGGCCGTGCA GCTCGCGAAC GACACGCCGT ACGGCCTCGG CGCGAGCATC TGGTCGAACG ATCTGTCCGC GATCCACAAG CTGGTCCCGC GTATTGCCGC GGGCACCGTG TGGGTCAACT GCCACTCGCT GCTCGACAAC GCGCTGCCGT TCGGCGGGAT GAAGCAGTCG GGCTTCGGCC GCGAACTCGG TCGCGCGGTG ATCGACCAGT ACACGGAAAG CAAGTCGGTG ATGATGAACT ACGCGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT