BuceA.00020.n

Putative NADP-dependent glyceraldehyde-3-phosphate dehydrogenase

CENTER ID: BuceA.00020.n
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00020.n.B1.GE36445 Full length( BuceA.00020.n ) 1 484
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.4914
RefSeq: YP_002233802.1
UniProt: B4EGU1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTVLKPIPA SDVRHEALRI DGQRVWRDAV IDVRNPYDGA LVGTVPKATL DDVRRAFAVA RAYRPTLTRH DRAAILRRAA DIVRSRTAEI AALITAEAGL CIKDSTYEAG RVADVLTFGA GEVLKDDGQI FSCDLTPHGK KRRVYTQRDP LLGVISAITP FNHPMNQVAH KIVPSVATNN RIVVKPSEKV PLSCYLFADI LYEAGLPPQM LQVITGDPKE IADELITNPA IDLITFTGGV SIGKSIAARM GYRRAVLELG GNDPIIVMED ADLDEASTLA VSGSYKNSGQ RCTAIKRMLV HEAVADRFTA LVVEKTRAWS YGNPSDPSVD MGTVIDEAAA KFCEQQVNDA IARGARLLVG NVRDGALYSP TVVDRVTPDM PLVKHETFGP VSPIMRFRDI DEAIRMSNST DYALSSSVCT NRFDHITRFI TELEVGSVNV REVPGYRLEL TPFGGVKDSG LGYKEGVQEA MKSFTNVKTY SLPW
NT Sequence
ATGAGCACGG TTTTGAAGCC GATTCCCGCA TCCGACGTGC GTCACGAAGC GTTGCGCATC GACGGGCAGC GCGTGTGGCG CGACGCGGTG ATCGACGTGC GCAACCCGTA CGACGGCGCG CTGGTCGGCA CCGTGCCGAA GGCGACGCTC GATGACGTGC GCCGTGCCTT CGCGGTCGCG CGTGCGTACC GGCCGACGCT CACGCGCCAC GATCGCGCGG CGATCCTGCG CCGCGCGGCC GACATCGTGC GCTCGCGCAC CGCGGAGATC GCGGCGCTGA TCACGGCCGA GGCCGGTTTG TGCATCAAGG ATTCGACTTA CGAGGCCGGC CGCGTGGCCG ACGTGCTCAC GTTCGGCGCC GGCGAAGTGC TGAAGGACGA CGGGCAGATC TTCTCGTGCG ATCTGACGCC GCACGGCAAG AAGCGCCGCG TGTACACGCA GCGCGATCCG CTGCTCGGCG TGATTTCGGC GATCACGCCG TTCAACCACC CGATGAACCA GGTCGCGCAC AAGATCGTGC CGTCGGTCGC GACCAACAAC CGGATCGTCG TGAAGCCGTC GGAGAAGGTG CCGCTGTCGT GTTATCTGTT CGCGGACATC CTGTACGAAG CCGGCCTGCC GCCGCAGATG CTGCAGGTGA TCACCGGCGA CCCGAAGGAA ATCGCCGACG AGCTGATCAC GAACCCGGCG ATCGACCTGA TCACGTTCAC CGGCGGCGTG TCGATCGGCA AGTCGATCGC CGCGCGGATG GGCTACCGGC GCGCGGTGCT CGAACTCGGC GGCAACGATC CGATCATCGT GATGGAAGAC GCCGATCTCG ATGAAGCGAG CACGCTCGCG GTATCGGGCT CGTACAAGAA TTCGGGGCAG CGCTGCACGG CGATCAAGCG GATGCTCGTG CACGAGGCGG TGGCCGATCG CTTCACGGCG CTCGTCGTCG AGAAGACGCG CGCCTGGTCG TACGGCAACC CGTCCGATCC GTCGGTCGAC ATGGGCACGG TGATCGACGA AGCGGCGGCG AAGTTCTGCG AGCAGCAAGT CAACGACGCG ATCGCGCGCG GCGCGCGGCT GCTGGTCGGC AACGTGCGCG ACGGCGCGCT GTATTCGCCG ACCGTCGTCG ATCGCGTGAC GCCCGACATG CCGCTGGTCA AGCACGAAAC CTTCGGGCCG GTGTCGCCGA TCATGCGCTT TCGCGATATC GACGAGGCGA TCCGGATGTC GAACAGCACC GACTACGCGC TGTCGTCGTC GGTGTGCACG AACCGCTTCG ACCACATCAC GCGCTTCATC ACCGAGCTGG AAGTCGGCAG CGTGAACGTG CGCGAAGTGC CCGGCTATCG GCTCGAACTG ACGCCGTTCG GCGGCGTGAA GGATTCGGGG CTCGGCTACA AGGAGGGCGT GCAGGAGGCG ATGAAGAGCT TCACGAACGT GAAGACGTAT TCGCTGCCGT GG
Details for BuceA.00020.n.B1.GE36445
HARVESTED ON: 11/21/2012
SEQUENCED ON: 11/27/2012
EXPECTED MW: 53kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 63
Validated AA Sequence
MNQVAHKIVP SVATNNRIVV KPSEKVPLSC YLFADILYEA GLPPQMLQVI TGDPKEIADE LITNPAIDLI TFTGGVSIGK SIAARMGYRR AVLELGGNDP IIVMEDADLD EASTLAVSGS YKNSGQRCTA IKRMLVHEAV ADRFTALVVE KTRAWSYGNP SDPSVDMGTV IDEAAAKFCE QQVNDAIARG ARLLVGNVRD GALYSPTVVD RVTPDMPLVK HETFGPVSPI MRFRDIDEAI RMSNSTDYAL SSSVCTNRFD HITRFITELE VGSVNVREVP GYRLELTPFG GVKDSGLGYK EGVQEAMKSF T
Validated NT Sequence
gttcgtgaag ctcttcatcg cctcctgcac gccctccttg tagccgagcc ccgaatcctt cacgccgccg aacggcgtca gttcgagccg atagccgggc acttcgcgca cgttcacgct gccgacttcc agctcggtga tgaagcgcgt gatgtggtcg aagcggttcg tgcacaccga cgacgacagc gcgtagtcgg tgctgttcga catccggatc gcctcgtcga tatcgcgaaa gcgcatgatc ggcgacaccg gcccgaaggt ttcgtgcttg accagcggca tgtcgggcgt cacgcgatcg acgacggtcg gcgaatacag cgcgccgtcg cgcacgttgc cgaccagcag ccgcgcgccg cgcgcgatcg cgtcgttgac ttgctgctcg cagaacttcg ccgccgcttc gtcgatcacc gtgcccatgt cgaccgacgg atcggacggg ttgccgtacg accaggcgcg cgtcttctcg acgacgagcg ccgtgaagcg atcggccacc gcctcgtgca cgagcatccg cttgatcgcc gtgcagcgct gccccgaatt cttgtacgag cccgataccg cgagcgtgct cgcttcatcg agatcggcgt cttccatcac gatgatcgga tcgttgccgc cgagttcgag caccgcgcgc cggtagccca tccgcgcggc gatcgacttg ccgatcgaca cgccgccggt gaacgtgatc aggtcgatcg ccgggttcgt gatcagctcg tcggcgattt ccttcgggtc gccggtgatc acctgcagca tctgcggcgg caggccggct tcgtacagga tgtccgcgaa cagataacac gacagcggca ccttctccga cggcttcacg acgatccggt tgttggtcgc gaccgacggc acgatcttgt gcgcgacctg gttcatcggg tggttgaacg gcgtgatcgc cgaaatcacg ccgagcagcg gatcgcgctg cgtgtacacg cggcgcttct tgccgtgcgg cgtcagatcg cacgagaaga tctgcccgtc gtccttcagc acttcgccgg cgccgaacgt gagcacgtcg gccacgcggc cggcctcgta agtcgaatcc ttgatgcaca aaccggcctc ggccgtgatc agcgccgcga tctccgcggt gcgcgagcgc acgatgtcgg ccgcgcggcg caggatcgcc gcgcgatcgt ggcgcgtgag cgtcggccgg tacgcacgcg cgaccgcgaa ggcacggcgc acgtcatcga gcgtcgcctt cggcacggtg ccgaccagcg cgccgtcgta cgggttgcgc acgtcgatca ccgcgtcgcg ccacacgcgc tgcccgtcga tgcgcaacgc ttcgtgacgc acgtcggatg cgggaatcgg cttcaaaacc
Expected Protein Sequence
MAHHHHHHMS TVLKPIPASD VRHEALRIDG QRVWRDAVID VRNPYDGALV GTVPKATLDD VRRAFAVARA YRPTLTRHDR AAILRRAADI VRSRTAEIAA LITAEAGLCI KDSTYEAGRV ADVLTFGAGE VLKDDGQIFS CDLTPHGKKR RVYTQRDPLL GVISAITPFN HPMNQVAHKI VPSVATNNRI VVKPSEKVPL SCYLFADILY EAGLPPQMLQ VITGDPKEIA DELITNPAID LITFTGGVSI GKSIAARMGY RRAVLELGGN DPIIVMEDAD LDEASTLAVS GSYKNSGQRC TAIKRMLVHE AVADRFTALV VEKTRAWSYG NPSDPSVDMG TVIDEAAAKF CEQQVNDAIA RGARLLVGNV RDGALYSPTV VDRVTPDMPL VKHETFGPVS PIMRFRDIDE AIRMSNSTDY ALSSSVCTNR FDHITRFITE LEVGSVNVRE VPGYRLELTP FGGVKDSGLG YKEGVQEAMK SFTNVKTYSL PW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca cggttttgaa gccgattccc gcatccgacg tgcgtcacga agcgttgcgc atcgacgggc agcgcgtgtg gcgcgacgcg gtgatcgacg tgcgcaaccc gtacgacggc gcgctggtcg gcaccgtgcc gaaggcgacg ctcgatgacg tgcgccgtgc cttcgcggtc gcgcgtgcgt accggccgac gctcacgcgc cacgatcgcg cggcgatcct gcgccgcgcg gccgacatcg tgcgctcgcg caccgcggag atcgcggcgc tgatcacggc cgaggccggt ttgtgcatca aggattcgac ttacgaggcc ggccgcgtgg ccgacgtgct cacgttcggc gccggcgaag tgctgaagga cgacgggcag atcttctcgt gcgatctgac gccgcacggc aagaagcgcc gcgtgtacac gcagcgcgat ccgctgctcg gcgtgatttc ggcgatcacg ccgttcaacc acccgatgaa ccaggtcgcg cacaagatcg tgccgtcggt cgcgaccaac aaccggatcg tcgtgaagcc gtcggagaag gtgccgctgt cgtgttatct gttcgcggac atcctgtacg aagccggcct gccgccgcag atgctgcagg tgatcaccgg cgacccgaag gaaatcgccg acgagctgat cacgaacccg gcgatcgacc tgatcacgtt caccggcggc gtgtcgatcg gcaagtcgat cgccgcgcgg atgggctacc ggcgcgcggt gctcgaactc ggcggcaacg atccgatcat cgtgatggaa gacgccgatc tcgatgaagc gagcacgctc gcggtatcgg gctcgtacaa gaattcgggg cagcgctgca cggcgatcaa gcggatgctc gtgcacgagg cggtggccga tcgcttcacg gcgctcgtcg tcgagaagac gcgcgcctgg tcgtacggca acccgtccga tccgtcggtc gacatgggca cggtgatcga cgaagcggcg gcgaagttct gcgagcagca agtcaacgac gcgatcgcgc gcggcgcgcg gctgctggtc ggcaacgtgc gcgacggcgc gctgtattcg ccgaccgtcg tcgatcgcgt gacgcccgac atgccgctgg tcaagcacga aaccttcggg ccggtgtcgc cgatcatgcg ctttcgcgat atcgacgagg cgatccggat gtcgaacagc accgactacg cgctgtcgtc gtcggtgtgc acgaaccgct tcgaccacat cacgcgcttc atcaccgagc tggaagtcgg cagcgtgaac gtgcgcgaag tgcccggcta tcggctcgaa ctgacgccgt tcggcggcgt gaaggattcg gggctcggct acaaggaggg cgtgcaggag gcgatgaaga gcttcacgaa cgtgaagacg tattcgctgc cgtggtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat