BuceA.00020.m

Methylmalonate-semialdehyde dehydrogenase

CENTER ID: BuceA.00020.m
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BuceA.00020.m.B1.GE36444 Full length( BuceA.00020.m ) 1 508
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.4870
RefSeq: YP_002233767.1
UniProt: B4EGQ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNAAVPQTTL ALPTAKLLID GAFVDSRSAE WGDIVNPATQ EVIGRVPYAT LDEVDAAIAS AQRAFQTWKT TPIGARLRIM LKFQDLVRRN LERIAHTLTA EQGKTLPDAQ GDIFRGLEVV EHACSIGTLQ QGEFAENVAG GVDTYTLRQP IGVCAGITPF NFPGMIPLWM FPMAIVCGNT FVLKPSEQDP LSTMQLVELA IEAGVPPGVL NVVHGGKTVV DRLCTHPDIK AISFVGSTRV GTHVYNLGSQ HGKRVQSMMG AKNHAVVLPD AHREQSINAL VGAGFGAAGQ RCMATSVVVL VGQARDWLPD LVEKAKALKV NAGAEAGTDV GPVVSKAAKA RILSLIDAGV KEGATLLLDG RDVKVPGYEH GNFVGPTIFS GVTTDMSIYQ EEIFGPVVVV MEAGTLDDAI ALVNRNPMGN GVGLFTQSGA AARKFQSEID VGQVGINIPI PVPVPYFSFT GSRGSKLGDL GPYGKQVVQF YTQTKTVTAR WFDDDATAGG VNTTIALR
NT Sequence
ATGAACGCCG CAGTTCCCCA GACCACCCTC GCCCTGCCCA CCGCCAAGCT GCTGATCGAC GGCGCGTTCG TCGATTCGCG CAGTGCCGAA TGGGGCGACA TCGTCAACCC CGCGACGCAG GAAGTCATCG GCCGCGTGCC GTACGCGACG CTCGACGAGG TCGATGCCGC GATCGCCTCC GCGCAGCGCG CGTTCCAGAC CTGGAAGACG ACGCCGATCG GCGCGCGGCT GCGCATCATG CTGAAGTTCC AGGATCTCGT GCGCCGCAAT CTCGAACGCA TCGCGCACAC GCTGACGGCC GAGCAAGGCA AGACGCTGCC CGACGCGCAG GGCGACATCT TCCGCGGCCT CGAGGTGGTC GAGCACGCGT GCTCGATCGG CACGCTGCAG CAAGGCGAAT TCGCGGAGAA CGTCGCGGGC GGCGTCGATA CCTACACGCT GCGCCAGCCC ATCGGCGTCT GCGCGGGCAT CACGCCGTTC AATTTCCCCG GGATGATTCC GCTGTGGATG TTCCCGATGG CGATCGTGTG CGGCAACACG TTCGTGCTGA AGCCGTCCGA GCAGGACCCG CTGTCGACGA TGCAGCTCGT CGAACTCGCG ATCGAAGCCG GCGTGCCGCC AGGCGTGCTG AACGTCGTGC ACGGCGGCAA GACGGTGGTC GACCGCCTGT GCACGCACCC GGACATCAAG GCGATCTCGT TCGTCGGCTC GACGCGCGTC GGCACGCACG TGTACAACCT CGGCAGCCAG CACGGCAAGC GCGTGCAGTC GATGATGGGC GCGAAGAACC ACGCGGTCGT GCTGCCCGAT GCGCATCGCG AGCAGTCGAT CAACGCACTC GTCGGCGCGG GCTTCGGCGC GGCCGGCCAG CGCTGCATGG CGACGTCGGT CGTCGTGCTG GTCGGCCAGG CGCGCGACTG GCTGCCCGAT CTCGTCGAAA AGGCGAAGGC GTTGAAGGTC AATGCGGGCG CGGAAGCCGG CACCGACGTC GGCCCGGTCG TGTCGAAGGC GGCGAAGGCG CGCATCCTGT CGCTGATCGA CGCCGGCGTG AAGGAAGGCG CGACGCTGCT GCTCGACGGC CGCGACGTGA AGGTGCCCGG CTACGAGCAC GGCAACTTCG TCGGCCCGAC GATCTTCTCC GGCGTGACGA CCGACATGTC GATCTACCAG GAAGAAATCT TCGGGCCGGT GGTGGTCGTG ATGGAAGCCG GCACGCTCGA CGACGCGATC GCGCTCGTCA ACCGCAACCC GATGGGCAAC GGCGTGGGCC TGTTCACGCA GAGCGGCGCG GCCGCGCGCA AGTTCCAGAG CGAGATCGAC GTCGGCCAGG TCGGCATCAA CATCCCGATT CCGGTGCCGG TGCCCTACTT CAGCTTCACC GGTTCGCGCG GCTCGAAGCT CGGCGATCTC GGCCCGTACG GCAAGCAGGT CGTGCAGTTC TACACGCAGA CCAAGACGGT CACCGCGCGC TGGTTCGACG ACGATGCGAC GGCCGGCGGC GTGAACACGA CGATCGCGCT GCGT
Details for BuceA.00020.m.B1.GE36444
HARVESTED ON: 11/21/2012
SEQUENCED ON: 11/27/2012
EXPECTED MW: 54kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 20
PERCENT COVERAGE: 90
Validated AA Sequence
MGRHRQPRDA GSHRPRAVRD ARRGRCRDRL RAARVPDLED DADRRAAAHH AEVPGSRAPQ SRTHRAHADG RARQDAARRA GRHLPRPRGG RARVLDRHAA ARRIRGERRG RRRYLHAAPA HRRLRGHHAV QFPRDDSAVD VPDGDRVRQH VRAEAVRAGP AVDDAARRTR DRSRRAARRA ERRARRQDGG RPPVHAPGHQ GDLVRRLDAR RHARVQPRQP ARQARAVDDG REEPRGRAAR CASRAVDQRT RRRGLRRGRP ALHGDVGRRA GRPGARLAAR SRRKGEGVEG QCGRGSRHRR RPGRVEGGEG AHPVADRRRR EGRRDAAARR PRREGARLRA RQLRRPDDLL RRDDRHVDLP GRNLRAGGGR DGSRHARRRD RARQPQPDGQ RRGPVHAERR GRAQVPERDR RRPGRHQHPD SGAGALLQLH RFARLEARRS RPVRQAGRAV LHADQDGHRA LVRRRCDGR
Validated NT Sequence
ccgccggccg tcgcatcgtc gtcgaaccag cgcgcggtga ccgtcttggt ctgcgtgtag aactgcacga cctgcttgcc gtacgggccg agatcgccga gcttcgagcc gcgcgaaccg gtgaagctga agtagggcac cggcaccgga atcgggatgt tgatgccgac ctggccgacg tcgatctcgc tctggaactt gcgcgcggcc gcgccgctct gcgtgaacag gcccacgccg ttgcccatcg ggttgcggtt gacgagcgcg atcgcgtcgt cgagcgtgcc ggcttccatc acgaccacca ccggcccgaa gatttcttcc tggtagatcg acatgtcggt cgtcacgccg gagaagatcg tcgggccgac gaagttgccg tgctcgtagc cgggcacctt cacgtcgcgg ccgtcgagca gcagcgtcgc gccttccttc acgccggcgt cgatcagcga caggatgcgc gccttcgccg ccttcnacac gaccgggccg acgtcggtgc cggcttccgc gcccgcattg accttcaacg ccttcgcctt ttcgacgaga tcgggcagcc agtcgcgcgc ctggccgacc agcacgacga ccgacgtcgc catgcagcgc tggccggccg cgccgaagcc cgcgccgacg agtgcgttga tcgactgctc gcgatgcgca tcgggcagca cgaccgcgtg gttcttcgcg cccatcatcg actgcacgcg cttgccgtgc tggctgccga ggttgtacac gtgcgtgccg acgcgcgtcg agccgacgaa cgagatcgcc ttgatgtccg ggtgcgtgca caggcggtcg accaccgtct tgccgccgtg cacgacgttc agcacgcctg gcggcacgcc ggcttcgatc gcgagttcga cgagctgcat cgtcgacagc gggtcctgct cggacggctt cagcacgaac gtgttgccgc acacgatcgc catcgggaac atccacagcg gaatcatccc ggggaaattg aacggcgtga tgcccgcgca gacgccgatg ggctggcgca gcgtgtaggt atcgacgccg cccgcgacgt tctccgcgaa ttcgccttgc tgcagcgtgc cgatcgagca cgcgtgctcg accacctcga ggccgcggaa gatgtcgccc tgcgcgtcgg gcagcgtctt gccttgctcg gccgtcagcg tgtgcgcgat gcgttcgaga ttgcggcgca cgagatcctg gaacttcagc atgatgcgca gccgcgcgcc gatcggcgtc gtcttccagg tctggaacgc gcgctgcgcg gaggcgatcg cggcatcgac ctcgtcgagc gtcgcgtacg gcacgcggcc gatgacttcc tgcgtcgcgg ggttgacgat gtcgccccat tcggcactgc gcgaatcgac gaacgcgccg tcgatcagca gcttggcggt gggcagggcg agggtggtct ggggaactgc gg
Expected Protein Sequence
MAHHHHHHMN AAVPQTTLAL PTAKLLIDGA FVDSRSAEWG DIVNPATQEV IGRVPYATLD EVDAAIASAQ RAFQTWKTTP IGARLRIMLK FQDLVRRNLE RIAHTLTAEQ GKTLPDAQGD IFRGLEVVEH ACSIGTLQQG EFAENVAGGV DTYTLRQPIG VCAGITPFNF PGMIPLWMFP MAIVCGNTFV LKPSEQDPLS TMQLVELAIE AGVPPGVLNV VHGGKTVVDR LCTHPDIKAI SFVGSTRVGT HVYNLGSQHG KRVQSMMGAK NHAVVLPDAH REQSINALVG AGFGAAGQRC MATSVVVLVG QARDWLPDLV EKAKALKVNA GAEAGTDVGP VVSKAAKARI LSLIDAGVKE GATLLLDGRD VKVPGYEHGN FVGPTIFSGV TTDMSIYQEE IFGPVVVVME AGTLDDAIAL VNRNPMGNGV GLFTQSGAAA RKFQSEIDVG QVGINIPIPV PVPYFSFTGS RGSKLGDLGP YGKQVVQFYT QTKTVTARWF DDDATAGGVN TTIALR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg ccgcagttcc ccagaccacc ctcgccctgc ccaccgccaa gctgctgatc gacggcgcgt tcgtcgattc gcgcagtgcc gaatggggcg acatcgtcaa ccccgcgacg caggaagtca tcggccgcgt gccgtacgcg acgctcgacg aggtcgatgc cgcgatcgcc tccgcgcagc gcgcgttcca gacctggaag acgacgccga tcggcgcgcg gctgcgcatc atgctgaagt tccaggatct cgtgcgccgc aatctcgaac gcatcgcgca cacgctgacg gccgagcaag gcaagacgct gcccgacgcg cagggcgaca tcttccgcgg cctcgaggtg gtcgagcacg cgtgctcgat cggcacgctg cagcaaggcg aattcgcgga gaacgtcgcg ggcggcgtcg atacctacac gctgcgccag cccatcggcg tctgcgcggg catcacgccg ttcaatttcc ccgggatgat tccgctgtgg atgttcccga tggcgatcgt gtgcggcaac acgttcgtgc tgaagccgtc cgagcaggac ccgctgtcga cgatgcagct cgtcgaactc gcgatcgaag ccggcgtgcc gccaggcgtg ctgaacgtcg tgcacggcgg caagacggtg gtcgaccgcc tgtgcacgca cccggacatc aaggcgatct cgttcgtcgg ctcgacgcgc gtcggcacgc acgtgtacaa cctcggcagc cagcacggca agcgcgtgca gtcgatgatg ggcgcgaaga accacgcggt cgtgctgccc gatgcgcatc gcgagcagtc gatcaacgca ctcgtcggcg cgggcttcgg cgcggccggc cagcgctgca tggcgacgtc ggtcgtcgtg ctggtcggcc aggcgcgcga ctggctgccc gatctcgtcg aaaaggcgaa ggcgttgaag gtcaatgcgg gcgcggaagc cggcaccgac gtcggcccgg tcgtgtcgaa ggcggcgaag gcgcgcatcc tgtcgctgat cgacgccggc gtgaaggaag gcgcgacgct gctgctcgac ggccgcgacg tgaaggtgcc cggctacgag cacggcaact tcgtcggccc gacgatcttc tccggcgtga cgaccgacat gtcgatctac caggaagaaa tcttcgggcc ggtggtggtc gtgatggaag ccggcacgct cgacgacgcg atcgcgctcg tcaaccgcaa cccgatgggc aacggcgtgg gcctgttcac gcagagcggc gcggccgcgc gcaagttcca gagcgagatc gacgtcggcc aggtcggcat caacatcccg attccggtgc cggtgcccta cttcagcttc accggttcgc gcggctcgaa gctcggcgat ctcggcccgt acggcaagca ggtcgtgcag ttctacacgc agaccaagac ggtcaccgcg cgctggttcg acgacgatgc gacggccggc ggcgtgaaca cgacgatcgc gctgcgttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at