BuceA.00020.l

Methylmalonate-semialdehyde dehydrogenase

CENTER ID: BuceA.00020.l
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00020.l.B1.GE36443 Full length( BuceA.00020.l ) 1 509
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00020.l.B1.PS01828 Full length( BuceA.00020.l ) 1 509

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.5973
RefSeq: YP_002234794.1
UniProt: B4EGC6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNANPTPRTG QDVPTVKLLI DGAFVESATN EWRDIVNPAT QQVLARVPFA TVAEVDAAVQ AAHAAYATWK NTPIAARMRI MLKFQDLVRA NQQRIAKTLT AEQGKTIPDA EGDIFRGLEV VEHACSIGTL QLGEFAENVA GGVDTYTLRQ PLGVCAGITP FNFPAMIPLW MFPMAIVCGN TFVLKPSEQD PMSTMELVEL ALEAGVPKGV LNVVHGGKEV VDAICTHPLV KAISFVGSTA VGTHVYNLGS QHGKRVQSMM GAKNHAIVLP DANREQTINA LVGAGFGAAG QRCMATSVAV LVGNAREWLP DIVAKAKTLK VNAGSEAGTD VGPVVSRGAK ARILSLIDMG IKEGAKLELD GRDVKVAGFE EGNFIGPTIF SGVTTDMTVY THEIFGPVLV VMEVDTLDEA IALVNANPMG NGVGIFTQSG AAARKFQSEI DVGQVGINIP IPVPVPFFSF TGSRGSKLGD LGPYGKQVVQ FYTQTKTVTA RWFDDDTTAG PVNTTIRLH
NT Sequence
ATGAACGCGA ATCCGACGCC CCGCACGGGG CAAGACGTGC CGACGGTCAA GCTGCTGATC GACGGCGCCT TCGTCGAGTC CGCCACGAAC GAGTGGCGCG ACATCGTCAA CCCGGCGACG CAGCAGGTGC TCGCACGCGT GCCGTTCGCG ACCGTCGCGG AAGTCGACGC GGCCGTGCAG GCCGCGCACG CCGCGTATGC GACGTGGAAG AACACGCCGA TCGCCGCGCG CATGCGCATC ATGCTGAAGT TCCAGGATCT GGTGCGCGCG AACCAGCAGC GCATCGCGAA GACGCTGACG GCCGAGCAGG GCAAGACGAT TCCCGATGCC GAAGGCGACA TCTTCCGCGG CCTCGAAGTG GTCGAGCACG CATGCTCGAT CGGCACGCTG CAGCTCGGCG AATTCGCGGA GAACGTCGCG GGCGGCGTCG ACACCTACAC GCTGCGCCAG CCGCTCGGCG TGTGCGCGGG CATCACGCCG TTCAATTTTC CCGCGATGAT TCCGCTGTGG ATGTTCCCGA TGGCGATCGT GTGCGGCAAC ACGTTCGTGC TGAAGCCGTC CGAGCAGGAC CCGATGTCGA CGATGGAGCT CGTCGAGCTC GCGCTGGAAG CCGGCGTACC GAAAGGCGTG CTGAACGTCG TGCACGGCGG CAAGGAAGTC GTCGATGCGA TCTGCACGCA TCCGCTCGTG AAGGCGATCT CGTTCGTCGG CTCGACCGCC GTCGGCACGC ACGTGTACAA CCTCGGCAGC CAGCACGGCA AGCGCGTGCA GTCGATGATG GGCGCGAAGA ACCACGCGAT CGTGCTGCCC GACGCGAACC GCGAGCAGAC GATCAACGCG CTCGTCGGCG CGGGCTTCGG CGCGGCCGGC CAGCGCTGCA TGGCGACTTC CGTCGCGGTG CTGGTGGGCA ACGCGCGCGA ATGGCTGCCC GACATCGTCG CGAAGGCGAA GACGCTGAAG GTCAACGCGG GCTCGGAAGC GGGCACCGAC GTCGGGCCGG TCGTGTCGCG CGGCGCGAAG GCGCGGATTC TGTCGCTGAT CGACATGGGC ATCAAGGAAG GCGCGAAGCT CGAACTCGAC GGCCGCGACG TGAAGGTCGC CGGCTTCGAG GAAGGCAACT TCATCGGCCC GACGATCTTC TCCGGCGTGA CGACCGACAT GACGGTCTAC ACGCATGAAA TCTTCGGACC GGTGCTGGTC GTGATGGAAG TCGACACGCT CGACGAAGCG ATCGCGCTGG TCAACGCGAA CCCGATGGGC AACGGCGTGG GCATCTTCAC GCAGAGCGGC GCGGCGGCAC GCAAGTTCCA GAGCGAGATC GACGTCGGCC AGGTTGGCAT CAACATCCCG ATCCCGGTGC CCGTGCCGTT CTTCAGCTTC ACCGGTTCGC GCGGCTCGAA GCTCGGCGAT CTCGGCCCGT ACGGCAAGCA GGTCGTGCAG TTCTACACGC AGACCAAGAC GGTCACCGCG CGCTGGTTCG ACGACGACAC GACGGCCGGC CCGGTGAATA CGACGATCCG GCTGCAC
Details for BuceA.00020.l.B1.GE36443
HARVESTED ON: 11/21/2012
SEQUENCED ON: 11/27/2012
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 82
Validated AA Sequence
MRIMLKFQDL VRANQQRIAK TLTAEQGKTI PDAEGDIFRG LEVVEHACSI GTLQLGEFAE NVAGGVDTYT LRQPLGVCAG ITPFNFPAMI PLWMFPMAIV CGNTFVLKPS EQDPMSTMEL VELALEAGVP KGVLNVVHGG KEVVXAICTH PLVKAISFVG STAVGTHVYN LGSQHGKRVQ SMMGAKNHAI VLPDANREQT INALVGAGFG AAGQRCMATS VAVLVGNARE WLPDIVAKAK TLKVNAGSEA GTDVGPVVSR GAKARILSLI DMGIKEGAKL ELDGRDVKVA GFEEGNFIGP TIFSGVTTDM TVYTHEIFGP VLVVMEVDTL DEAIALVNAN PMGNGVGIFT QSGAAARKFQ SEIDVGQVGI NIPIPVPVPF FSFTGSRGSK LGDLGPYGKQ VVQFYTQTKT VTARWFDDDT TAGP
Validated NT Sequence
ccgggccggc cgtcgtgtcg tcgtcgaacc agcgcgcggt gaccgtcttg gtctgcgtgt agaactgcac gacctgcttg ccgtacgggc cgagatcgcc gagcttcgag ccgcgcgaac cggtgaagct gaagaacggc acgggcaccg ggatcgggat gttgatgcca acctggccga cgtcgatctc gctctggaac ttgcgtgccg ccgcgccgct ctgcgtgaag atgcccacgc cgttgcccat cgggttcgcg ttgaccagcg cgatcgcttc gtcgagcgtg tcgacttcca tcacgaccag caccggtccg aagatttcat gcgtgtagac cgtcatgtcg gtcgtcacgc cggagaagat cgtcgggccg atgaagttgc cttcctcgaa gccggcgacc ttcacgtcgc ggccgtcgag ttcgagcttc gcgccttcct tgatgcccat gtcgatcagc gacagaatcc gcgccttcgc gccgcgcgac acgaccggcc cgacgtcggt gcccgcttcc gagcccgcgt tgaccttcag cgtcttcgcc ttcgcgacga tgtcgggcag ccattcgcgc gcgttgccca ccagcaccgc gacggaagtc gccatgcagc gctggccggc cgcgccgaan cccgcgccga cgagcgcgtt gatcgtctgc tcgcggttcg cgtcgggcag cacgatcgcg tggttcttcg cgcccatcat cgactgcacg cgcttgccgt gctggctgcc gaggttgtac acgtgcgtgc cgacggcggt cgagccgacg aacgagatcg ccttcacgag cggatgcgtg cagatcgcat ngacgacttc cttgccgccg tgcacgacgt tcagcacgcc tttcggtacg ccggcttcca gcgcgagctc gacgagctcc atcgtcgaca tcgggtcctg ctcggacggc ttcagcacga acgtgttgcc gcacacgatc gccatcggga acatccacag cggaatcatc gcgggaaaat tgaacggcgt gatgcccgcg cacacgccga gcggctggcg cagcgtgtag gtgtcgacgc cgcccgcgac gttctccgcg aattcgccga gctgcagcgt gccgatcgag catgcgtgct cgaccacttc gaggccgcgg aagatgtcgc cttcggcatc gggaatcgtc ttgccctgct cggccgtcag cgtcttcgcg atgcgctgct ggttcgcgcg caccagatcc tggaacttca gcatgatgcg catgcgcgcg gcgatcggcg tgttcttcca cgtcgcatac gcggcgtgcg cggcctgcac ggccgcgtcg acttccgcga cggtcgcgaa cggcacgcgt gcgagcacct gctgcgtcgc cgggttgacg atgtcgcgcc actcgttcgt ggcggactcg acgaaggcgc cgtcgatcag cagcttgacc gtcggcacgt cttgccccgt gcggggcgtc ggattc
Expected Protein Sequence
MAHHHHHHMN ANPTPRTGQD VPTVKLLIDG AFVESATNEW RDIVNPATQQ VLARVPFATV AEVDAAVQAA HAAYATWKNT PIAARMRIML KFQDLVRANQ QRIAKTLTAE QGKTIPDAEG DIFRGLEVVE HACSIGTLQL GEFAENVAGG VDTYTLRQPL GVCAGITPFN FPAMIPLWMF PMAIVCGNTF VLKPSEQDPM STMELVELAL EAGVPKGVLN VVHGGKEVVD AICTHPLVKA ISFVGSTAVG THVYNLGSQH GKRVQSMMGA KNHAIVLPDA NREQTINALV GAGFGAAGQR CMATSVAVLV GNAREWLPDI VAKAKTLKVN AGSEAGTDVG PVVSRGAKAR ILSLIDMGIK EGAKLELDGR DVKVAGFEEG NFIGPTIFSG VTTDMTVYTH EIFGPVLVVM EVDTLDEAIA LVNANPMGNG VGIFTQSGAA ARKFQSEIDV GQVGINIPIP VPVPFFSFTG SRGSKLGDLG PYGKQVVQFY TQTKTVTARW FDDDTTAGPV NTTIRLH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg cgaatccgac gccccgcacg gggcaagacg tgccgacggt caagctgctg atcgacggcg ccttcgtcga gtccgccacg aacgagtggc gcgacatcgt caacccggcg acgcagcagg tgctcgcacg cgtgccgttc gcgaccgtcg cggaagtcga cgcggccgtg caggccgcgc acgccgcgta tgcgacgtgg aagaacacgc cgatcgccgc gcgcatgcgc atcatgctga agttccagga tctggtgcgc gcgaaccagc agcgcatcgc gaagacgctg acggccgagc agggcaagac gattcccgat gccgaaggcg acatcttccg cggcctcgaa gtggtcgagc acgcatgctc gatcggcacg ctgcagctcg gcgaattcgc ggagaacgtc gcgggcggcg tcgacaccta cacgctgcgc cagccgctcg gcgtgtgcgc gggcatcacg ccgttcaatt ttcccgcgat gattccgctg tggatgttcc cgatggcgat cgtgtgcggc aacacgttcg tgctgaagcc gtccgagcag gacccgatgt cgacgatgga gctcgtcgag ctcgcgctgg aagccggcgt accgaaaggc gtgctgaacg tcgtgcacgg cggcaaggaa gtcgtcgatg cgatctgcac gcatccgctc gtgaaggcga tctcgttcgt cggctcgacc gccgtcggca cgcacgtgta caacctcggc agccagcacg gcaagcgcgt gcagtcgatg atgggcgcga agaaccacgc gatcgtgctg cccgacgcga accgcgagca gacgatcaac gcgctcgtcg gcgcgggctt cggcgcggcc ggccagcgct gcatggcgac ttccgtcgcg gtgctggtgg gcaacgcgcg cgaatggctg cccgacatcg tcgcgaaggc gaagacgctg aaggtcaacg cgggctcgga agcgggcacc gacgtcgggc cggtcgtgtc gcgcggcgcg aaggcgcgga ttctgtcgct gatcgacatg ggcatcaagg aaggcgcgaa gctcgaactc gacggccgcg acgtgaaggt cgccggcttc gaggaaggca acttcatcgg cccgacgatc ttctccggcg tgacgaccga catgacggtc tacacgcatg aaatcttcgg accggtgctg gtcgtgatgg aagtcgacac gctcgacgaa gcgatcgcgc tggtcaacgc gaacccgatg ggcaacggcg tgggcatctt cacgcagagc ggcgcggcgg cacgcaagtt ccagagcgag atcgacgtcg gccaggttgg catcaacatc ccgatcccgg tgcccgtgcc gttcttcagc ttcaccggtt cgcgcggctc gaagctcggc gatctcggcc cgtacggcaa gcaggtcgtg cagttctaca cgcagaccaa gacggtcacc gcgcgctggt tcgacgacga cacgacggcc ggcccggtga atacgacgat ccggctgcac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuceA.00020.l.B1.PS01828
PURIFICATION DATe: 5/22/2013
CONCENTRATION: 44mg/ml
OBSERVED MW: 57kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 82
Protocol Notes
notes unavailable
Validated AA Sequence
MRIMLKFQDL VRANQQRIAK TLTAEQGKTI PDAEGDIFRG LEVVEHACSI GTLQLGEFAE NVAGGVDTYT LRQPLGVCAG ITPFNFPAMI PLWMFPMAIV CGNTFVLKPS EQDPMSTMEL VELALEAGVP KGVLNVVHGG KEVVXAICTH PLVKAISFVG STAVGTHVYN LGSQHGKRVQ SMMGAKNHAI VLPDANREQT INALVGAGFG AAGQRCMATS VAVLVGNARE WLPDIVAKAK TLKVNAGSEA GTDVGPVVSR GAKARILSLI DMGIKEGAKL ELDGRDVKVA GFEEGNFIGP TIFSGVTTDM TVYTHEIFGP VLVVMEVDTL DEAIALVNAN PMGNGVGIFT QSGAAARKFQ SEIDVGQVGI NIPIPVPVPF FSFTGSRGSK LGDLGPYGKQ VVQFYTQTKT VTARWFDDDT TAGP
Validated NT Sequence
ccgggccggc cgtcgtgtcg tcgtcgaacc agcgcgcggt gaccgtcttg gtctgcgtgt agaactgcac gacctgcttg ccgtacgggc cgagatcgcc gagcttcgag ccgcgcgaac cggtgaagct gaagaacggc acgggcaccg ggatcgggat gttgatgcca acctggccga cgtcgatctc gctctggaac ttgcgtgccg ccgcgccgct ctgcgtgaag atgcccacgc cgttgcccat cgggttcgcg ttgaccagcg cgatcgcttc gtcgagcgtg tcgacttcca tcacgaccag caccggtccg aagatttcat gcgtgtagac cgtcatgtcg gtcgtcacgc cggagaagat cgtcgggccg atgaagttgc cttcctcgaa gccggcgacc ttcacgtcgc ggccgtcgag ttcgagcttc gcgccttcct tgatgcccat gtcgatcagc gacagaatcc gcgccttcgc gccgcgcgac acgaccggcc cgacgtcggt gcccgcttcc gagcccgcgt tgaccttcag cgtcttcgcc ttcgcgacga tgtcgggcag ccattcgcgc gcgttgccca ccagcaccgc gacggaagtc gccatgcagc gctggccggc cgcgccgaan cccgcgccga cgagcgcgtt gatcgtctgc tcgcggttcg cgtcgggcag cacgatcgcg tggttcttcg cgcccatcat cgactgcacg cgcttgccgt gctggctgcc gaggttgtac acgtgcgtgc cgacggcggt cgagccgacg aacgagatcg ccttcacgag cggatgcgtg cagatcgcat ngacgacttc cttgccgccg tgcacgacgt tcagcacgcc tttcggtacg ccggcttcca gcgcgagctc gacgagctcc atcgtcgaca tcgggtcctg ctcggacggc ttcagcacga acgtgttgcc gcacacgatc gccatcggga acatccacag cggaatcatc gcgggaaaat tgaacggcgt gatgcccgcg cacacgccga gcggctggcg cagcgtgtag gtgtcgacgc cgcccgcgac gttctccgcg aattcgccga gctgcagcgt gccgatcgag catgcgtgct cgaccacttc gaggccgcgg aagatgtcgc cttcggcatc gggaatcgtc ttgccctgct cggccgtcag cgtcttcgcg atgcgctgct ggttcgcgcg caccagatcc tggaacttca gcatgatgcg catgcgcgcg gcgatcggcg tgttcttcca cgtcgcatac gcggcgtgcg cggcctgcac ggccgcgtcg acttccgcga cggtcgcgaa cggcacgcgt gcgagcacct gctgcgtcgc cgggttgacg atgtcgcgcc actcgttcgt ggcggactcg acgaaggcgc cgtcgatcag cagcttgacc gtcggcacgt cttgccccgt gcggggcgtc ggattc
Expressed Protein Sequence
MAHHHHHHMN ANPTPRTGQD VPTVKLLIDG AFVESATNEW RDIVNPATQQ VLARVPFATV AEVDAAVQAA HAAYATWKNT PIAARMRIML KFQDLVRANQ QRIAKTLTAE QGKTIPDAEG DIFRGLEVVE HACSIGTLQL GEFAENVAGG VDTYTLRQPL GVCAGITPFN FPAMIPLWMF PMAIVCGNTF VLKPSEQDPM STMELVELAL EAGVPKGVLN VVHGGKEVVD AICTHPLVKA ISFVGSTAVG THVYNLGSQH GKRVQSMMGA KNHAIVLPDA NREQTINALV GAGFGAAGQR CMATSVAVLV GNAREWLPDI VAKAKTLKVN AGSEAGTDVG PVVSRGAKAR ILSLIDMGIK EGAKLELDGR DVKVAGFEEG NFIGPTIFSG VTTDMTVYTH EIFGPVLVVM EVDTLDEAIA LVNANPMGNG VGIFTQSGAA ARKFQSEIDV GQVGINIPIP VPVPFFSFTG SRGSKLGDLG PYGKQVVQFY TQTKTVTARW FDDDTTAGPV NTTIRLH
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACG CGAATCCGAC GCCCCGCACG GGGCAAGACG TGCCGACGGT CAAGCTGCTG ATCGACGGCG CCTTCGTCGA GTCCGCCACG AACGAGTGGC GCGACATCGT CAACCCGGCG ACGCAGCAGG TGCTCGCACG CGTGCCGTTC GCGACCGTCG CGGAAGTCGA CGCGGCCGTG CAGGCCGCGC ACGCCGCGTA TGCGACGTGG AAGAACACGC CGATCGCCGC GCGCATGCGC ATCATGCTGA AGTTCCAGGA TCTGGTGCGC GCGAACCAGC AGCGCATCGC GAAGACGCTG ACGGCCGAGC AGGGCAAGAC GATTCCCGAT GCCGAAGGCG ACATCTTCCG CGGCCTCGAA GTGGTCGAGC ACGCATGCTC GATCGGCACG CTGCAGCTCG GCGAATTCGC GGAGAACGTC GCGGGCGGCG TCGACACCTA CACGCTGCGC CAGCCGCTCG GCGTGTGCGC GGGCATCACG CCGTTCAATT TTCCCGCGAT GATTCCGCTG TGGATGTTCC CGATGGCGAT CGTGTGCGGC AACACGTTCG TGCTGAAGCC GTCCGAGCAG GACCCGATGT CGACGATGGA GCTCGTCGAG CTCGCGCTGG AAGCCGGCGT ACCGAAAGGC GTGCTGAACG TCGTGCACGG CGGCAAGGAA GTCGTCGATG CGATCTGCAC GCATCCGCTC GTGAAGGCGA TCTCGTTCGT CGGCTCGACC GCCGTCGGCA CGCACGTGTA CAACCTCGGC AGCCAGCACG GCAAGCGCGT GCAGTCGATG ATGGGCGCGA AGAACCACGC GATCGTGCTG CCCGACGCGA ACCGCGAGCA GACGATCAAC GCGCTCGTCG GCGCGGGCTT CGGCGCGGCC GGCCAGCGCT GCATGGCGAC TTCCGTCGCG GTGCTGGTGG GCAACGCGCG CGAATGGCTG CCCGACATCG TCGCGAAGGC GAAGACGCTG AAGGTCAACG CGGGCTCGGA AGCGGGCACC GACGTCGGGC CGGTCGTGTC GCGCGGCGCG AAGGCGCGGA TTCTGTCGCT GATCGACATG GGCATCAAGG AAGGCGCGAA GCTCGAACTC GACGGCCGCG ACGTGAAGGT CGCCGGCTTC GAGGAAGGCA ACTTCATCGG CCCGACGATC TTCTCCGGCG TGACGACCGA CATGACGGTC TACACGCATG AAATCTTCGG ACCGGTGCTG GTCGTGATGG AAGTCGACAC GCTCGACGAA GCGATCGCGC TGGTCAACGC GAACCCGATG GGCAACGGCG TGGGCATCTT CACGCAGAGC GGCGCGGCGG CACGCAAGTT CCAGAGCGAG ATCGACGTCG GCCAGGTTGG CATCAACATC CCGATCCCGG TGCCCGTGCC GTTCTTCAGC TTCACCGGTT CGCGCGGCTC GAAGCTCGGC GATCTCGGCC CGTACGGCAA GCAGGTCGTG CAGTTCTACA CGCAGACCAA GACGGTCACC GCGCGCTGGT TCGACGACGA CACGACGGCC GGCCCGGTGA ATACGACGAT CCGGCTGCAC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT