BuceA.00020.k
2-aminomuconate 6-semialdehyde dehydrogenase
| CENTER ID: | BuceA.00020.k |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00020.k.B1.GE36442 | Full length( BuceA.00020.k ) | 1 | 496 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4U3W
DEPOSITED: 7/23/2014
DETERMINATION: XRay
CLONE: BuceA.00020.k.B1.GE36442
PROTEIN: BuceA.00020.k.B1.PW37245
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_132910 |
| PATRIC ID: | fig|216591.5.peg.5903 |
| RefSeq: | YP_002234732.1 |
| UniProt: | B4EFS9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTQLNSTSR DRQLLRHYIN GEFVASGTTF PNLSPVDGRK LADVCEADAA LVDSAVQAAH AAQKAGWRDT TPAQRAAWLH KIADGIEARF DEFVAAEVAD TGRPVAQART LDIARGIANF RTFADLVRTA SGEYFETHAA DGSELINYVT RKPLGVIGII SPWNLPLLLF TWKVAPALAM GNCVVAKPSE ETPSSATLLA EVMHDVGLPP GVFNLIHGHG QNAAGEFLTR HPDISAITFT GESRTGSTIM KAVADGVKEV SFELGGKNAA VVFADADFDA AVAGVLRSSF TNAGQVCLCS ERVYVERPIF ERFVAALKEQ AQALRVGAPE DPATTMGPLI SRGHRDKVLS YFRLAVEEGA TVVTGGGAPS FGDARDDGAF VMPTIWTGLP DSARCVREEI FGPVCHIAPF DDEAEVVKRV NDSAYGLAAS IWTTQLARGH RVAKQIETGI VWVNAWFVRD LRTPFGGTKL SGLGREGGRH SLDFYSELTN VCVRIA
NT Sequence
GTGACAACTC AATTGAATTC GACCTCGCGC GACCGGCAGT TGTTGCGCCA TTACATCAAC GGTGAGTTTG TGGCGAGCGG CACAACCTTT CCAAACCTCA GTCCGGTCGA CGGTCGCAAG CTTGCCGACG TTTGCGAAGC AGATGCGGCG CTCGTGGATA GCGCCGTACA GGCGGCTCAC GCGGCGCAGA AGGCCGGCTG GCGCGACACG ACGCCCGCGC AACGGGCGGC ATGGCTGCAT AAGATCGCGG ACGGCATCGA GGCCCGCTTC GACGAATTCG TGGCGGCGGA AGTCGCCGAT ACCGGTCGGC CTGTCGCGCA GGCCCGCACC CTCGACATCG CACGTGGCAT TGCCAATTTC CGGACCTTTG CCGACCTTGT CCGCACCGCG AGCGGTGAGT ACTTCGAAAC GCATGCGGCG GACGGCAGCG AGCTGATCAA CTACGTGACG CGCAAGCCGC TCGGCGTGAT CGGCATCATA TCGCCGTGGA ACCTGCCTCT GCTGTTGTTC ACGTGGAAGG TGGCGCCGGC GCTGGCAATG GGCAACTGTG TGGTCGCCAA GCCCTCCGAG GAGACACCCA GCTCCGCAAC GTTGCTCGCC GAAGTCATGC ACGACGTCGG CTTGCCACCC GGCGTCTTCA ATCTGATTCA TGGGCACGGC CAGAACGCGG CCGGCGAATT CCTGACGCGT CATCCGGATA TCAGTGCAAT CACGTTCACG GGCGAGTCGC GCACCGGCAG CACGATCATG AAGGCCGTTG CGGACGGCGT GAAAGAGGTT TCGTTCGAGC TGGGCGGCAA GAACGCGGCC GTCGTGTTCG CGGACGCGGA CTTCGATGCC GCGGTTGCCG GCGTCCTTCG GTCCAGTTTC ACGAACGCCG GCCAGGTCTG CCTGTGCAGT GAGCGGGTGT ATGTGGAGCG GCCGATCTTC GAGCGCTTCG TCGCGGCGCT CAAGGAACAG GCGCAAGCAT TGCGCGTTGG CGCGCCGGAA GATCCCGCCA CGACGATGGG ACCGCTCATT TCGCGCGGCC ATCGCGACAA GGTGCTGTCG TATTTTCGTC TTGCCGTCGA AGAAGGCGCG ACGGTCGTGA CCGGTGGTGG CGCCCCGAGC TTCGGCGATG CACGGGACGA CGGCGCGTTT GTGATGCCGA CGATCTGGAC GGGCTTGCCG GATTCCGCCC GATGCGTACG GGAAGAGATT TTCGGACCGG TGTGCCACAT CGCGCCGTTC GACGACGAAG CGGAGGTCGT GAAGCGCGTC AACGACAGTG CTTATGGTCT TGCCGCAAGC ATCTGGACGA CGCAGCTTGC GCGCGGCCAT CGCGTCGCGA AGCAGATCGA GACGGGCATC GTGTGGGTCA ATGCGTGGTT CGTGCGCGAT CTGCGCACAC CCTTCGGTGG CACCAAACTG TCAGGACTGG GACGCGAGGG GGGCCGCCAT TCGCTCGACT TCTATTCCGA ATTGACGAAC GTTTGCGTGA GGATTGCA
Details for BuceA.00020.k.B1.GE36442
| HARVESTED ON: | 11/21/2012 |
| SEQUENCED ON: | 11/27/2012 |
| EXPECTED MW: | 54kDa |
| OBSERVED MW: | 54kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 21 |
| PERCENT COVERAGE: | 86 |
Validated AA Sequence
MAARKLRRPD ACGKTISTVV DALHDLRFVV ERRDVAHRSE NLFPYASGGI RQARPDRRHH KRAVVPCIAE ARGATTGHDR RAFFDGKTKI RQHLVAMAAR NERSHRRGGI FRRANAQCLR LFLERRDEAL EDRPLHIHPL TAQADLAGVR ETGPKDAGNR GIEVRVREHD GRVLAAQLER NLFHAVRNGL HDRAAGARLA RERDCTDIRM TRQEFAGRVL AVPMNQIEDA GWQADVVHDF GEQRCGAGCL LGGLGDHTVA HCQRRRHLPR EQQQRQVPRR YDADHAERLA RHVVDQLAAV RRMRFEVLTA RGADKVGKGP EIGNATCDVE GAGLRDRPTG IGDFRRHEFV EAGLDAVRDL MQPCRPLRGR RVAPAGLLRR VSRLYGAIHE RRICFANVGK LATVDRTEVW KGCAARHKLT VDVMAQQLPV ARGRIQL
Validated NT Sequence
tcaattcgga atagaagtcg agcgaatggc ggcccccctc gcgtcccagt cctgacagtt tggtgccacc gaagggtgtg cgcagatcgc gcacgaacca cgcattgacc cacacgatgc ccgtctcgat ctgcttcgcg acgcgatggc cgcgcgcaag ctgcgtcgtc cagatgcttg cggcaagacc ataagcactg tcgttgacgc gcttcacgac ctccgcttcg tcgtcgaacg gcgcgatgtg gcacaccggt ccgaaaatct cttcccgtac gcatcgggcg gaatccggca agcccgtcca gatcgtcggc atcacaaacg cgccgtcgtc ccgtgcatcg ccgaagctcg gggcgccacc accggtcacg accgtcgcgc cttcttcgac ggcaagacga aaatacgaca gcaccttgtc gcgatggccg cgcgaaatga gcggtcccat cgtcgtggcg ggatcttccg gcgcgccaac gcgcaatgct tgcgcctgtt ccttgagcgc cgcgacgaag cgctcgaaga tcggccgctc cacatacacc cgctcactgc acaggcagac ctggccggcg ttcgtgaaac tggaccgaag gacgccggca accgcggcat cgaagtccgc gtccgcgaac acgacggccg cgttcttgcc gcccagctcg aacgaaacct ctttcacgcc gtccgcaacg gccttcatga tcgtgctgcc ggtgcgcgac tcgcccgtga acgtgattgc actgatatcc ggatgacgcg tcaggaattc gccggccgcg ttctggccgt gcccatgaat cagattgaag acgccgggtg gcaagcngac gtcgtgcatg acttcggcga gcaacgttgc ggagctgggt gtctcctcgg agggcttggc gaccacacag ttgcccattg ccagcgccgg cgccaccttc cacgtgaaca acagcagagg caggttccac ggcgatatga tgccgatcac gccgagcggc ttgcgcgtca cgtagttgat cagctcgctg ccgtccgccg catgcgtttc gaagtactca ccgctcgcgg tgcggacaag gtcggcaaag gtccggaaat tggcaatgcc acgtgcgatg tcgagggtgc gggcctgcgc gacaggccga ccggtatcgg cgacttccgc cgccacgaat tcgtcgaagc gggcctcgat gccgtccgcg atcttatgca gccatgccgc ccgttgcgcg ggcgtcgtgt cgcgccagcc ggccttctgc gccgcgtgag ccgcctgtac ggcgctatcc acgagcgccg catctgcttc gcaaacgtcg gcaagcttgc gaccgtcgac cggactgagg tttggaaagg ttgtgccgct cgccacaaac tcaccgttga tgtaatggcg caacaactgc cggtcgcgcg aggtcgaatt caattgagt
Expected Protein Sequence
MAHHHHHHMV TTQLNSTSRD RQLLRHYING EFVASGTTFP NLSPVDGRKL ADVCEADAAL VDSAVQAAHA AQKAGWRDTT PAQRAAWLHK IADGIEARFD EFVAAEVADT GRPVAQARTL DIARGIANFR TFADLVRTAS GEYFETHAAD GSELINYVTR KPLGVIGIIS PWNLPLLLFT WKVAPALAMG NCVVAKPSEE TPSSATLLAE VMHDVGLPPG VFNLIHGHGQ NAAGEFLTRH PDISAITFTG ESRTGSTIMK AVADGVKEVS FELGGKNAAV VFADADFDAA VAGVLRSSFT NAGQVCLCSE RVYVERPIFE RFVAALKEQA QALRVGAPED PATTMGPLIS RGHRDKVLSY FRLAVEEGAT VVTGGGAPSF GDARDDGAFV MPTIWTGLPD SARCVREEIF GPVCHIAPFD DEAEVVKRVN DSAYGLAASI WTTQLARGHR VAKQIETGIV WVNAWFVRDL RTPFGGTKLS GLGREGGRHS LDFYSELTNV CVRIA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga caactcaatt gaattcgacc tcgcgcgacc ggcagttgtt gcgccattac atcaacggtg agtttgtggc gagcggcaca acctttccaa acctcagtcc ggtcgacggt cgcaagcttg ccgacgtttg cgaagcagat gcggcgctcg tggatagcgc cgtacaggcg gctcacgcgg cgcagaaggc cggctggcgc gacacgacgc ccgcgcaacg ggcggcatgg ctgcataaga tcgcggacgg catcgaggcc cgcttcgacg aattcgtggc ggcggaagtc gccgataccg gtcggcctgt cgcgcaggcc cgcaccctcg acatcgcacg tggcattgcc aatttccgga cctttgccga ccttgtccgc accgcgagcg gtgagtactt cgaaacgcat gcggcggacg gcagcgagct gatcaactac gtgacgcgca agccgctcgg cgtgatcggc atcatatcgc cgtggaacct gcctctgctg ttgttcacgt ggaaggtggc gccggcgctg gcaatgggca actgtgtggt cgccaagccc tccgaggaga cacccagctc cgcaacgttg ctcgccgaag tcatgcacga cgtcggcttg ccacccggcg tcttcaatct gattcatggg cacggccaga acgcggccgg cgaattcctg acgcgtcatc cggatatcag tgcaatcacg ttcacgggcg agtcgcgcac cggcagcacg atcatgaagg ccgttgcgga cggcgtgaaa gaggtttcgt tcgagctggg cggcaagaac gcggccgtcg tgttcgcgga cgcggacttc gatgccgcgg ttgccggcgt ccttcggtcc agtttcacga acgccggcca ggtctgcctg tgcagtgagc gggtgtatgt ggagcggccg atcttcgagc gcttcgtcgc ggcgctcaag gaacaggcgc aagcattgcg cgttggcgcg ccggaagatc ccgccacgac gatgggaccg ctcatttcgc gcggccatcg cgacaaggtg ctgtcgtatt ttcgtcttgc cgtcgaagaa ggcgcgacgg tcgtgaccgg tggtggcgcc ccgagcttcg gcgatgcacg ggacgacggc gcgtttgtga tgccgacgat ctggacgggc ttgccggatt ccgcccgatg cgtacgggaa gagattttcg gaccggtgtg ccacatcgcg ccgttcgacg acgaagcgga ggtcgtgaag cgcgtcaacg acagtgctta tggtcttgcc gcaagcatct ggacgacgca gcttgcgcgc ggccatcgcg tcgcgaagca gatcgagacg ggcatcgtgt gggtcaatgc gtggttcgtg cgcgatctgc gcacaccctt cggtggcacc aaactgtcag gactgggacg cgaggggggc cgccattcgc tcgacttcta ttccgaattg acgaacgttt gcgtgaggat tgcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat