SSGCID: Target Page

BuceA.00020.j

Acetaldehyde dehydrogenase

CENTER ID:	BuceA.00020.j
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00020.j.B1.GE36441	Full length( BuceA.00020.j )		1	508

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00020.j.B1.PS01742	Full length( BuceA.00020.j )		1	508

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.73
RefSeq:	YP_002229228.1
UniProt:	B4EF17

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNPFDLKQLG LDVEYPYRQQ YDNYIGGKWV APVKGEYFEN VSPINGQPFC RIPRSGADDI ELALDAAHAA RRKWGKTSVA ERANLLLAAA DRMEKNLKLL AVAETIDNGK PLRETMAADL PLAIDHFRYF AGCIRAQEGG ISEIDDNTVA YHFHEPLGVV GQIIPWNFPL LMAAWKLAPA LAAGCCVVMK PAEQTPASVL VLMELIGDLF PAGTINIVNG FGKEAGEALA TSKRIAKIAF TGSTPVGKHI LRAAADSLIP STVELGGKSP NIFFADVLDQ DDAFLDKALE GLAMFALNQG EVCTCPSRIL IQESIYERFI EKAVARVERI KSGHPLDLQT MIGAQASQQQ LDKILSYIDI GRGEGAQCLT GGERAAPAAD LGTGYYVKPT MLLGNNKMRV FQEEIFGPVA SVMTFKDEQE AIELANDTFY GLGAGVWTRN GTRAYRMGRE VEAGRVWTNC YHLYPAHAAF GGYKQSGIGR ETHKMALSNY QQTKCLLVSY QAEALGFF

NT Sequence

ATGAACCCGT TTGACCTGAA GCAACTGGGC CTCGACGTCG AATACCCGTA CCGTCAGCAA TACGACAACT ACATCGGCGG CAAGTGGGTG GCGCCCGTGA AGGGCGAGTA TTTCGAGAAC GTGTCGCCGA TCAACGGCCA GCCGTTCTGC CGCATCCCGC GCTCCGGCGC CGACGACATC GAGCTGGCGC TCGACGCCGC GCATGCCGCG CGCCGCAAGT GGGGCAAGAC GTCCGTCGCC GAGCGCGCGA ACTTGCTGCT CGCCGCCGCC GACCGGATGG AAAAGAACCT GAAGCTGCTG GCCGTGGCCG AGACGATCGA CAACGGCAAG CCGCTGCGCG AGACGATGGC GGCCGACCTG CCGCTCGCGA TCGACCACTT CCGCTATTTC GCGGGCTGCA TCCGCGCGCA GGAAGGCGGC ATCTCCGAAA TCGACGACAA CACCGTCGCG TACCATTTCC ACGAGCCGCT CGGCGTGGTC GGCCAGATCA TCCCGTGGAA CTTCCCGCTG CTGATGGCCG CGTGGAAGCT CGCGCCGGCG CTCGCGGCCG GCTGCTGCGT GGTGATGAAG CCGGCCGAGC AGACGCCCGC GTCGGTGCTC GTGCTGATGG AGCTGATCGG CGACCTGTTC CCGGCCGGCA CGATCAACAT CGTGAACGGC TTCGGCAAGG AAGCGGGCGA AGCGCTCGCG ACCAGCAAGC GGATCGCGAA GATCGCGTTC ACGGGCTCGA CGCCGGTCGG CAAGCACATC CTGCGCGCGG CGGCCGACAG CCTGATCCCG TCGACGGTCG AGCTGGGCGG CAAGAGCCCG AACATCTTCT TCGCCGACGT GCTCGACCAG GACGACGCGT TCCTCGACAA GGCGCTCGAA GGGCTCGCGA TGTTCGCGCT GAACCAGGGC GAGGTGTGCA CGTGCCCGTC GCGGATCCTG ATCCAGGAAT CGATCTACGA ACGCTTCATC GAGAAGGCCG TCGCGCGCGT CGAGCGCATC AAGTCCGGCC ACCCGCTCGA CCTGCAGACG ATGATCGGCG CGCAGGCGTC GCAGCAGCAG CTCGACAAGA TCCTGTCATA CATCGACATC GGCCGCGGCG AAGGCGCGCA ATGCCTGACG GGCGGCGAGC GCGCGGCGCC GGCCGCCGAC CTCGGCACGG GCTACTACGT GAAGCCGACG ATGCTGCTCG GCAACAACAA GATGCGCGTG TTCCAGGAGG AGATCTTCGG GCCGGTCGCG TCGGTGATGA CGTTCAAGGA CGAGCAGGAA GCGATCGAAC TCGCGAACGA CACGTTCTAC GGGCTCGGCG CCGGCGTGTG GACGCGCAAC GGCACGCGTG CGTACCGGAT GGGCCGCGAG GTCGAGGCCG GCCGCGTGTG GACCAACTGC TACCACCTGT ACCCCGCGCA TGCGGCGTTC GGCGGCTACA AGCAGTCGGG GATCGGCCGC GAGACGCACA AGATGGCGCT GTCGAACTAT CAGCAGACGA AGTGCCTGCT GGTCAGCTAC CAGGCCGAGG CGCTCGGGTT CTTC

Details for BuceA.00020.j.B1.GE36441

HARVESTED ON:	11/21/2012
SEQUENCED ON:	11/27/2012
EXPECTED MW:	56kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	78

Validated AA Sequence

MEKNLKLLAV AETIDNGKPL RETMAADLPL AIDHFRYFAG CIRAQEGGIS EIDDNTVAYH FHEPLGVVGQ IIPWNFPLLM AAWKLAPALA AGCCVVMKPA EQTPASVLVL MELIGDLFPA GTINIVNGFG KEAGEALATS KRIAKIAFTG STPVGKHILR AAADSLIPST VELGGKSPNI FFADVLDQDD AFLDKALEGL AMFALNQGEV CTCPSRILIQ ESIYERFIEK AVARVERIKS GHPLDLQTMI GAQASQQQLD KILSYIDIGR GEGAQCLTGG ERAAPAADLG TGYYVKPTML LGNNKMRVFQ EEIFGPVASV MTFKDEQEAI ELANDTFYGL GAGVWTRNGT RAYRMGREVE AGRVWTNCYH LYPAHAAFGG YKQSGIGRET HKMALSNYQQ TKCLLVS

Validated NT Sequence

tagctgacca gcaggcactt cgtctgctga tagttcgaca gcgccatctt gtgcgtctcg cggccgatcc ccgactgctt gtagccgccg aacgccgcat gcgcggggta caggtggtag cagttggtcc acacgcggcc ggcctcgacc tcgcggccca tccggtacgc acgcgtgccg ttgcgcgtcc acacgccggc gccgagcccg tagaacgtgt cgttcgcgag ttcgatcgct tcctgctcgt ccttgaacgt catcaccgac gcgaccggcc cgaagatctc ctcctggaac acgcgcatct tgttgttgcc gagcagcatc gtcggcttca cgtagtagcc cgtgccgagg tcggcggccg gcgccgcgcg ctcgccgccc gtcaggcatt gcgcgccttc gccgcggccg atgtcgatgt atgacaggat cttgtcgagc tgctgctgcg acgcctgcgc gccgatcatc gtctgcaggt cgagcgggtg gccggacttg atgcgctcga cgcgcgcgac ggccttctcg atgaagcgtt cgtagatcga ttcctggatc aggatccgcg acgggcacgt gcacacctcg ccctggttca gcgcgaacat cgcgagccct tcgagcgcct tgtcgaggaa cgcgtcgtcc tggtcgagca cgtcggcgaa gaagatgttc gggctcttgc cgcccagctc gaccgtcgac gggatcaggc tgtcggccgc cgcgcgcagg atgtgcttgc cgaccggcgt cgagcccgtg aacgcgatct tcgcgatccg cttgctggtc gcgagcgctt cgcccgcttc cttgccgaag ccgttcacga tgttgatcgt gccggccggg aacaggtcgc cgatcagctc catcagcacg agcaccgacg cgggcgtctg ctcggccggc ttcatcacca cgcagcagcc ggccgcgagc gccggcgcga gcttccacgc ggccatcagc agcgggaagt tccacgggat gatctggccg accacgccga gcggctcgtg gaaatggtac gcgacggtgt tgtcgtcgat ttcggagatg ccgccttcct gcgcgcggat gcagcccgcg aaatagcgga agtggtcgat cgcgagcggc aggtcggccg ccatcgtctc gcgcagcggc ttgccgttgt cgatcgtctc ggccacggcc agcagcttca ggttcttttc catccggtcg gcggcggcga gcagcaagtt cgcgcgctcg gcgacggacg tcttgcccca cttgcggcgc gcggcatgcg cggcgtcgag cgccagctcg atgtcgtcgg cgccggagcg cgggatgcgg cagaacggct ggccgttgat cggcgacacg ttctcgaaat actcgccctt cacgggcgcc acccacttgc cgccgatgta gttgtcgtat tgctgacggt acgggtattc gacgtcgagg cccagttgct tcaggtcaaa cgg

Expected Protein Sequence

MAHHHHHHMN PFDLKQLGLD VEYPYRQQYD NYIGGKWVAP VKGEYFENVS PINGQPFCRI PRSGADDIEL ALDAAHAARR KWGKTSVAER ANLLLAAADR MEKNLKLLAV AETIDNGKPL RETMAADLPL AIDHFRYFAG CIRAQEGGIS EIDDNTVAYH FHEPLGVVGQ IIPWNFPLLM AAWKLAPALA AGCCVVMKPA EQTPASVLVL MELIGDLFPA GTINIVNGFG KEAGEALATS KRIAKIAFTG STPVGKHILR AAADSLIPST VELGGKSPNI FFADVLDQDD AFLDKALEGL AMFALNQGEV CTCPSRILIQ ESIYERFIEK AVARVERIKS GHPLDLQTMI GAQASQQQLD KILSYIDIGR GEGAQCLTGG ERAAPAADLG TGYYVKPTML LGNNKMRVFQ EEIFGPVASV MTFKDEQEAI ELANDTFYGL GAGVWTRNGT RAYRMGREVE AGRVWTNCYH LYPAHAAFGG YKQSGIGRET HKMALSNYQQ TKCLLVSYQA EALGFF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc cgtttgacct gaagcaactg ggcctcgacg tcgaataccc gtaccgtcag caatacgaca actacatcgg cggcaagtgg gtggcgcccg tgaagggcga gtatttcgag aacgtgtcgc cgatcaacgg ccagccgttc tgccgcatcc cgcgctccgg cgccgacgac atcgagctgg cgctcgacgc cgcgcatgcc gcgcgccgca agtggggcaa gacgtccgtc gccgagcgcg cgaacttgct gctcgccgcc gccgaccgga tggaaaagaa cctgaagctg ctggccgtgg ccgagacgat cgacaacggc aagccgctgc gcgagacgat ggcggccgac ctgccgctcg cgatcgacca cttccgctat ttcgcgggct gcatccgcgc gcaggaaggc ggcatctccg aaatcgacga caacaccgtc gcgtaccatt tccacgagcc gctcggcgtg gtcggccaga tcatcccgtg gaacttcccg ctgctgatgg ccgcgtggaa gctcgcgccg gcgctcgcgg ccggctgctg cgtggtgatg aagccggccg agcagacgcc cgcgtcggtg ctcgtgctga tggagctgat cggcgacctg ttcccggccg gcacgatcaa catcgtgaac ggcttcggca aggaagcggg cgaagcgctc gcgaccagca agcggatcgc gaagatcgcg ttcacgggct cgacgccggt cggcaagcac atcctgcgcg cggcggccga cagcctgatc ccgtcgacgg tcgagctggg cggcaagagc ccgaacatct tcttcgccga cgtgctcgac caggacgacg cgttcctcga caaggcgctc gaagggctcg cgatgttcgc gctgaaccag ggcgaggtgt gcacgtgccc gtcgcggatc ctgatccagg aatcgatcta cgaacgcttc atcgagaagg ccgtcgcgcg cgtcgagcgc atcaagtccg gccacccgct cgacctgcag acgatgatcg gcgcgcaggc gtcgcagcag cagctcgaca agatcctgtc atacatcgac atcggccgcg gcgaaggcgc gcaatgcctg acgggcggcg agcgcgcggc gccggccgcc gacctcggca cgggctacta cgtgaagccg acgatgctgc tcggcaacaa caagatgcgc gtgttccagg aggagatctt cgggccggtc gcgtcggtga tgacgttcaa ggacgagcag gaagcgatcg aactcgcgaa cgacacgttc tacgggctcg gcgccggcgt gtggacgcgc aacggcacgc gtgcgtaccg gatgggccgc gaggtcgagg ccggccgcgt gtggaccaac tgctaccacc tgtaccccgc gcatgcggcg ttcggcggct acaagcagtc ggggatcggc cgcgagacgc acaagatggc gctgtcgaac tatcagcaga cgaagtgcct gctggtcagc taccaggccg aggcgctcgg gttcttctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BuceA.00020.j.B1.PS01742

PURIFICATION DATe:	2/7/2013
CONCENTRATION:	28mg/ml
OBSERVED MW:	54kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	78

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACC CGTTTGACCT GAAGCAACTG GGCCTCGACG TCGAATACCC GTACCGTCAG CAATACGACA ACTACATCGG CGGCAAGTGG GTGGCGCCCG TGAAGGGCGA GTATTTCGAG AACGTGTCGC CGATCAACGG CCAGCCGTTC TGCCGCATCC CGCGCTCCGG CGCCGACGAC ATCGAGCTGG CGCTCGACGC CGCGCATGCC GCGCGCCGCA AGTGGGGCAA GACGTCCGTC GCCGAGCGCG CGAACTTGCT GCTCGCCGCC GCCGACCGGA TGGAAAAGAA CCTGAAGCTG CTGGCCGTGG CCGAGACGAT CGACAACGGC AAGCCGCTGC GCGAGACGAT GGCGGCCGAC CTGCCGCTCG CGATCGACCA CTTCCGCTAT TTCGCGGGCT GCATCCGCGC GCAGGAAGGC GGCATCTCCG AAATCGACGA CAACACCGTC GCGTACCATT TCCACGAGCC GCTCGGCGTG GTCGGCCAGA TCATCCCGTG GAACTTCCCG CTGCTGATGG CCGCGTGGAA GCTCGCGCCG GCGCTCGCGG CCGGCTGCTG CGTGGTGATG AAGCCGGCCG AGCAGACGCC CGCGTCGGTG CTCGTGCTGA TGGAGCTGAT CGGCGACCTG TTCCCGGCCG GCACGATCAA CATCGTGAAC GGCTTCGGCA AGGAAGCGGG CGAAGCGCTC GCGACCAGCA AGCGGATCGC GAAGATCGCG TTCACGGGCT CGACGCCGGT CGGCAAGCAC ATCCTGCGCG CGGCGGCCGA CAGCCTGATC CCGTCGACGG TCGAGCTGGG CGGCAAGAGC CCGAACATCT TCTTCGCCGA CGTGCTCGAC CAGGACGACG CGTTCCTCGA CAAGGCGCTC GAAGGGCTCG CGATGTTCGC GCTGAACCAG GGCGAGGTGT GCACGTGCCC GTCGCGGATC CTGATCCAGG AATCGATCTA CGAACGCTTC ATCGAGAAGG CCGTCGCGCG CGTCGAGCGC ATCAAGTCCG GCCACCCGCT CGACCTGCAG ACGATGATCG GCGCGCAGGC GTCGCAGCAG CAGCTCGACA AGATCCTGTC ATACATCGAC ATCGGCCGCG GCGAAGGCGC GCAATGCCTG ACGGGCGGCG AGCGCGCGGC GCCGGCCGCC GACCTCGGCA CGGGCTACTA CGTGAAGCCG ACGATGCTGC TCGGCAACAA CAAGATGCGC GTGTTCCAGG AGGAGATCTT CGGGCCGGTC GCGTCGGTGA TGACGTTCAA GGACGAGCAG GAAGCGATCG AACTCGCGAA CGACACGTTC TACGGGCTCG GCGCCGGCGT GTGGACGCGC AACGGCACGC GTGCGTACCG GATGGGCCGC GAGGTCGAGG CCGGCCGCGT GTGGACCAAC TGCTACCACC TGTACCCCGC GCATGCGGCG TTCGGCGGCT ACAAGCAGTC GGGGATCGGC CGCGAGACGC ACAAGATGGC GCTGTCGAAC TATCAGCAGA CGAAGTGCCT GCTGGTCAGC TACCAGGCCG AGGCGCTCGG GTTCTTCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT