BuceA.00020.i

Aldehyde dehydrogenase family protein

CENTER ID: BuceA.00020.i
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00020.i.B1.GE36440 Full length( BuceA.00020.i ) 1 452
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1230
RefSeq: YP_002230322.1
UniProt: B4EDR4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHFQSRNPAT GELLEAFTEH SADDIESRLM LSFAAWKRWS RASLQQRTEV LRKLGALLEE RADRYGRLIT QEMGKPLVEA VLEVRKAAAG ARHFAEHGAG YLAPMPIEGM NAKVVYESLG PVFGVMPWNL PFWQVLRFFI PTAMAGNTVL VKHAETVQGC AHALEQLILD AGAPEGLYLN LAIRRGGVAR VVRDPRVRCV TVTGSTQAGR AVAQEAGAYG KKAVLELGGS DPFIVLEDAD LDRAVQLGVT SRFSNNAQSC IAAKRFLVAE PIAEVFQKKF VEKAAALRMG DPMSVDTQLG PLARADLRDE IHRQVLSALK QGGTLLTGGA PVNGPGNFYP PTVITGLSSD APIAQEEIFG PVAMLFTFRT DEEAIALANA TEFGLGATVC SADVQRANKI ASALEVGAVF INDFVRSDPR APFGGVKGSG FGRELGALGA RELANAKLVV GG
NT Sequence
ATGCACTTTC AATCGCGAAA CCCCGCAACC GGTGAACTGC TCGAGGCCTT CACCGAACAT TCGGCCGACG ATATCGAGTC GCGGCTTATG CTGTCATTTG CAGCCTGGAA GCGCTGGTCG CGGGCTTCGC TGCAGCAGCG CACCGAGGTG CTGCGCAAGC TCGGTGCGCT GCTCGAAGAG CGCGCCGACC GGTATGGCCG CCTCATCACA CAGGAAATGG GCAAGCCGCT CGTTGAAGCC GTTCTGGAGG TCAGGAAGGC GGCCGCGGGG GCGCGCCATT TCGCGGAACA TGGCGCCGGC TATCTCGCGC CGATGCCTAT CGAAGGCATG AACGCGAAGG TCGTCTATGA GTCGCTCGGC CCTGTGTTTG GTGTGATGCC GTGGAATTTG CCTTTCTGGC AAGTGCTGCG CTTTTTCATT CCGACGGCCA TGGCCGGGAA CACAGTGCTG GTCAAGCATG CTGAAACAGT GCAAGGCTGT GCGCACGCGC TCGAGCAATT GATCCTCGAC GCGGGTGCGC CTGAAGGCCT GTACCTGAAT CTCGCCATTC GCCGCGGCGG CGTGGCGCGC GTGGTGCGCG ATCCCCGCGT GCGATGCGTC ACCGTAACCG GCAGCACGCA GGCGGGGCGC GCCGTGGCCC AGGAAGCCGG TGCGTACGGA AAGAAAGCCG TTCTGGAGCT GGGCGGATCT GACCCGTTCA TCGTGCTGGA AGATGCGGAT CTGGACCGGG CAGTTCAGTT GGGGGTTACC TCGCGCTTTT CGAATAACGC GCAGAGCTGT ATCGCGGCCA AACGCTTTCT GGTTGCGGAG CCGATCGCTG AAGTGTTCCA GAAGAAGTTC GTGGAAAAGG CTGCGGCGCT GCGCATGGGC GATCCGATGA GCGTCGACAC GCAACTCGGG CCGCTTGCCA GGGCGGACCT GCGCGACGAA ATCCATCGGC AGGTACTTTC CGCGCTCAAG CAGGGTGGCA CGCTACTGAC CGGCGGTGCA CCGGTCAATG GTCCCGGCAA CTTCTACCCG CCAACCGTTA TCACTGGGCT GAGTTCCGAC GCCCCCATTG CGCAGGAGGA AATCTTCGGT CCGGTAGCCA TGCTGTTCAC GTTCAGGACC GACGAAGAGG CGATCGCGCT GGCTAATGCG ACCGAATTCG GTTTGGGTGC GACCGTTTGC TCCGCCGACG TGCAACGCGC AAACAAGATC GCGTCCGCTC TCGAAGTGGG AGCGGTATTC ATCAACGACT TCGTGCGCTC GGACCCTCGG GCGCCTTTTG GTGGCGTAAA AGGCTCGGGA TTCGGGCGGG AGCTGGGTGC ACTCGGCGCA CGAGAACTCG CAAACGCAAA GCTGGTAGTG GGTGGC
Details for BuceA.00020.i.B1.GE36440
HARVESTED ON: 11/21/2012
SEQUENCED ON: 11/27/2012
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 18
PERCENT COVERAGE: 37
Validated AA Sequence
MNGSDPPSSR TAFFPYAPAS WATARPACVL PVTVTHRTRG SRTTRATPPR RMARFXYRPS XAPASRXNCS SACAQPCTVS XXLTSTVFPX XXXRNEKXXT CXXANSTXSX XXXXXLXXDX ASCXXXASXX XXXXXXXKXX XPXXXXFXXX XXXXXXXGXX XXXXXXXXXX XXXX
Validated NT Sequence
cnttcgggct ttgttagcag ccggatccta tcttactcac ttagccaccc actaccagct ttgcgtttgc gagttctcgt gcgccgagtg cacccagctc ccgcccgaat cccgagcctt ttacgccacc aaaaggcgcc cgagggtccg agcgcacgaa gtcgttgatg aataccgctc ccacttcgag agcggacgcg atcttgtttg cgcgttgcac gtcggcggag caaacggtcg cacccaaacc gaattcggtc gcattagcca gcgcgatcgc ctcttcgtcg gtcctgaacg tgaacagcat ggctaccgga ccgaagattt cctcctgcgc aatgggggcg tcggaactca gcccagtgat aacggttggc gggtagaagt tgccgggacc attgaccggt gcaccgccgg tcagtagcgt gccaccctgc ttgagcgcgg aaagtacctg ccgatggatt tcgtcgcgca ggtccgccct ggcaagcggc ccgagttgcg tgtcgacgct catcggatcg cccatgcgca gcgccgcagc cttttccacg aacttcttct ggaacacttc agcgatcggc tccgcaacca gaaagcgttt ggccgcgata cagctctgcg cgttattcga aaagcgcgag gtaaccccca actgaactgc ccggtccaga tccgcatctt ccagcacgat gaacgggtca gatccgccca gctccagaac ggctttcttt ccgtacgcac cggcttcctg ggccacggcg cgccccgcct gcgtgctgcc ggttacggtg acgcatcgca cgcggggatc gcgcaccacg cgcgccacgc cgccgcggcg aatggcgaga ttcangtaca ggccttcnng cgcacccgcg tcgagganca attgctcgag cgcgtgcgca cagccttgca ctgtttcanc atgnttgacc agcactgtgt tcccngncnt ggncngtcgg aatgaaaagc ncancacttg ccanaangca aattccacgn catcacancn nnnacnnnan ngactcanan acgacnncgc gtcatgcntt cnnnnngcat cngncnnnnn nnnngngncn tgnnnnnnna aatgnnnnnn cccgannncn nnangnttca nnnnnnnnnn nnnnnttnnn nnnnnnnnng gcnnncngnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngnnnnn a
Expected Protein Sequence
MAHHHHHHMH FQSRNPATGE LLEAFTEHSA DDIESRLMLS FAAWKRWSRA SLQQRTEVLR KLGALLEERA DRYGRLITQE MGKPLVEAVL EVRKAAAGAR HFAEHGAGYL APMPIEGMNA KVVYESLGPV FGVMPWNLPF WQVLRFFIPT AMAGNTVLVK HAETVQGCAH ALEQLILDAG APEGLYLNLA IRRGGVARVV RDPRVRCVTV TGSTQAGRAV AQEAGAYGKK AVLELGGSDP FIVLEDADLD RAVQLGVTSR FSNNAQSCIA AKRFLVAEPI AEVFQKKFVE KAAALRMGDP MSVDTQLGPL ARADLRDEIH RQVLSALKQG GTLLTGGAPV NGPGNFYPPT VITGLSSDAP IAQEEIFGPV AMLFTFRTDE EAIALANATE FGLGATVCSA DVQRANKIAS ALEVGAVFIN DFVRSDPRAP FGGVKGSGFG RELGALGARE LANAKLVVGG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcact ttcaatcgcg aaaccccgca accggtgaac tgctcgaggc cttcaccgaa cattcggccg acgatatcga gtcgcggctt atgctgtcat ttgcagcctg gaagcgctgg tcgcgggctt cgctgcagca gcgcaccgag gtgctgcgca agctcggtgc gctgctcgaa gagcgcgccg accggtatgg ccgcctcatc acacaggaaa tgggcaagcc gctcgttgaa gccgttctgg aggtcaggaa ggcggccgcg ggggcgcgcc atttcgcgga acatggcgcc ggctatctcg cgccgatgcc tatcgaaggc atgaacgcga aggtcgtcta tgagtcgctc ggccctgtgt ttggtgtgat gccgtggaat ttgcctttct ggcaagtgct gcgctttttc attccgacgg ccatggccgg gaacacagtg ctggtcaagc atgctgaaac agtgcaaggc tgtgcgcacg cgctcgagca attgatcctc gacgcgggtg cgcctgaagg cctgtacctg aatctcgcca ttcgccgcgg cggcgtggcg cgcgtggtgc gcgatccccg cgtgcgatgc gtcaccgtaa ccggcagcac gcaggcgggg cgcgccgtgg cccaggaagc cggtgcgtac ggaaagaaag ccgttctgga gctgggcgga tctgacccgt tcatcgtgct ggaagatgcg gatctggacc gggcagttca gttgggggtt acctcgcgct tttcgaataa cgcgcagagc tgtatcgcgg ccaaacgctt tctggttgcg gagccgatcg ctgaagtgtt ccagaagaag ttcgtggaaa aggctgcggc gctgcgcatg ggcgatccga tgagcgtcga cacgcaactc gggccgcttg ccagggcgga cctgcgcgac gaaatccatc ggcaggtact ttccgcgctc aagcagggtg gcacgctact gaccggcggt gcaccggtca atggtcccgg caacttctac ccgccaaccg ttatcactgg gctgagttcc gacgccccca ttgcgcagga ggaaatcttc ggtccggtag ccatgctgtt cacgttcagg accgacgaag aggcgatcgc gctggctaat gcgaccgaat tcggtttggg tgcgaccgtt tgctccgccg acgtgcaacg cgcaaacaag atcgcgtccg ctctcgaagt gggagcggta ttcatcaacg acttcgtgcg ctcggaccct cgggcgcctt ttggtggcgt aaaaggctcg ggattcgggc gggagctggg tgcactcggc gcacgagaac tcgcaaacgc aaagctggta gtgggtggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat