BuceA.00020.h

Putative salicylaldehyde dehydrogenase

CENTER ID: BuceA.00020.h
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00020.h.B1.GE36439 Full length( BuceA.00020.h ) 1 509
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00020.h.B1.PW37264 Full length( BuceA.00020.h ) 1 509

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1200
RefSeq: YP_002230292.1
UniProt: B4EDN3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTATTPFHAP AGAGFLHEYG QFIDGKWTPG DTGKTIDLHN PATGDVLACI AAGNGKDAER AIDAAARAFP GWARTQPAAR QEILYEIMRR LKARQQYYAL MDTLNNGKPI SESMHFDMPM AIEQFAIFSG APWQLHGESM RHTSSLGTDS IGIVHREPLG VVVQIIPWNV PMIMLACKIA PALAAGNTIV LKPSEIVCLS VLEFAREMAD LLPPGVLNIV TGYGPDLGEA LVTDTRVRKV GFTGSRPTAR KLVQYAAANI IPQTMELGGK SANIVCEDAD LDAAAEGAAM STVLNKGEVC LAGSRLFVHE KVRDDFLDKL QRILAGIRIG DPTSPDTQLG PQASRMQMDK ILGYLEEGPR EGARVLTGGG RARVPGYDNG LFIQPTVFVD VKNSMKIAQE EIFGPVTSVL TWKDDDEVVR LANASVYGLG GGIWTANLAR AHRMACALET GTVWINRYYN FLGGMPIGGY KQSGFGREFA HEVLNHYTLT KSVIVDLQPG ALGIFGARQ
NT Sequence
ATGACCGCGA CCACCCCCTT CCACGCCCCT GCCGGCGCAG GCTTTCTTCA CGAATATGGC CAGTTCATCG ACGGCAAGTG GACGCCGGGC GATACCGGCA AAACGATCGA TCTGCACAAT CCGGCGACGG GCGACGTCCT CGCTTGCATC GCGGCCGGAA ACGGCAAGGA TGCCGAACGG GCCATCGATG CGGCTGCGCG CGCGTTCCCG GGCTGGGCGC GCACGCAGCC CGCCGCGCGT CAGGAGATCC TGTACGAGAT CATGCGCCGC CTCAAGGCCC GGCAGCAGTA CTACGCGCTC ATGGATACGC TGAACAATGG CAAGCCGATC AGTGAATCGA TGCACTTCGA CATGCCGATG GCGATCGAGC AGTTCGCGAT CTTCTCCGGC GCGCCGTGGC AACTGCACGG CGAATCGATG CGCCACACGT CGTCGCTCGG CACGGACTCG ATCGGCATCG TGCATCGCGA GCCGCTCGGC GTGGTCGTGC AGATCATTCC GTGGAACGTG CCGATGATCA TGCTCGCGTG CAAGATCGCG CCAGCCCTCG CCGCCGGTAA CACGATCGTG CTGAAGCCGT CCGAGATCGT ATGCCTGTCG GTGCTCGAAT TCGCTCGCGA GATGGCGGAC CTGCTGCCGC CCGGCGTCCT GAACATCGTG ACGGGCTACG GTCCGGATCT CGGCGAGGCG CTGGTGACCG ACACGCGCGT GCGCAAGGTC GGCTTCACCG GTTCGCGGCC GACCGCGCGC AAGCTCGTCC AGTACGCTGC GGCCAACATC ATCCCGCAAA CGATGGAACT CGGCGGAAAA TCGGCCAACA TCGTCTGCGA GGATGCCGAC CTCGACGCCG CCGCCGAAGG CGCGGCCATG TCGACGGTAC TGAACAAGGG CGAAGTCTGC CTCGCGGGCT CGCGCCTGTT CGTCCACGAA AAGGTGCGCG ATGATTTCCT CGACAAGCTC CAGCGCATTC TCGCCGGCAT TCGGATCGGT GACCCGACGT CGCCGGACAC GCAGCTCGGC CCCCAGGCAT CGCGGATGCA GATGGACAAA ATCCTCGGCT ATCTGGAAGA AGGCCCGCGC GAAGGCGCCC GGGTGCTGAC GGGCGGTGGC CGCGCCCGCG TGCCCGGCTA CGACAACGGA CTCTTCATTC AACCGACCGT GTTCGTCGAC GTGAAGAACT CGATGAAGAT CGCGCAGGAG GAAATCTTCG GCCCCGTCAC GAGCGTGCTC ACCTGGAAGG ACGACGACGA GGTCGTACGG TTGGCCAATG CATCCGTCTA CGGCCTGGGC GGCGGCATCT GGACCGCCAA CCTCGCGCGC GCCCATCGCA TGGCCTGCGC GCTGGAAACC GGCACGGTGT GGATCAACCG CTACTACAAC TTTCTCGGCG GCATGCCGAT CGGCGGCTAC AAGCAAAGCG GCTTCGGCCG CGAATTCGCG CACGAGGTGC TGAACCACTA CACGTTGACG AAGAGCGTGA TCGTCGACCT GCAGCCCGGC GCACTGGGCA TCTTCGGCGC ACGGCAA
Details for BuceA.00020.h.B1.GE36439
HARVESTED ON: 11/21/2012
SEQUENCED ON: 11/27/2012
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 19
PERCENT COVERAGE: 84
Validated AA Sequence
MGAREVGGPD AAAQAVDGCI GQPYDLVVVL PGEHARDGAE XFLLRDLHRV LHVDEHGRLN EESVVVAGHA GAATARQHPG AFARAFFQIA EDFVHLHPRC LGAELRVRRR RVTDPNAGEN ALELVEEIIX HLFVDEQARA READFALVXX XRHGRAFGGG VXVGILADDV GRFSAEFHRL RDDVGRSVLD ELARGRPRTG EADLAHARVG HQRLAEIRTV ARHDVQDAGR QQVRHLASEF EHRQAYDLGR LQHDRVTGGE GWRDLAREHD HRHVPRNDLH DHAERLAMHD ADRVRAERRR VAHRFAVQLP RRAGEDRELL DRHRHVEVHR FTDRLAIVQR IHERVVLLPG LEAAHDLVQD LLTRGGLRAR PARERARSRI DGPFGILAVS GRDASEDVAR RRIVQIDRFA GIARRPLAVD ELAIFVKKAC AGRGVEGGG
Validated NT Sequence
gcgccgggct gcaggtcgac gatcacgctc ttcgtcaacg tgtagtggtt cagcacctcg tgcgcgaatt cgcggccgaa gccgctttgc ttgtagccgc cgatcggcat gccgccgaga aagttgtagt agcggttgat ccacaccgtg ccggtttcca gcgcgcaggc catgcgatgg gcgcgcgcga ggttggcggt ccagatgccg ccgcccaggc cgtagacgga tgcattggcc aaccgtacga cctcgtcgtc gtccttccag gtgagcacgc tcgtgacggg gccgaanatt tcctcctgcg cgatcttcat cgagttcttc acgtcgacga acacggtcgg ttgaatgaag agtccgttgt cgtagccggg cacgcgggcg cggccaccgc ccgtcagcac ccgggcgcct tcgcgcgggc cttcttccag atagccgagg attttgtcca tctgcatccg cgatgcctgg gggccgagct gcgtgtccgg cgacgtcggg tcaccgatcc naatgccggc gagaatgcgc tggagcttgt cgaggaaatc atcnngcacc ttttcgtgga cgaacaggcg cgagcccgcg aggcagactt cgcccttgtt cantanngtc gacatggccg cgccttcggc ggcggcgtcn aggtcggcat cctcgcagac gatgttggnc gattttccgc cgagttccat cgtttgcggg atgatgttgg ccgcagcgta ctggacgagc ttgcgcgcgg tcggccgcga accggtgaag ccgaccttgc gcacgcgcgt gtcggtcacc agcgcctcgc cgagatccgg accgtagccc gtcacgatgt tcaggacgcc gggcggcagc aggtccgcca tctcgcgagc gaattcgagc accgacaggc atacgatctc ggacggcttc agcacgatcg tgttaccggc ggcgagggct ggcgcgatct tgcacgcgag catgatcatc ggcacgttcc acggaatgat ctgcacgacc acgccgagcg gctcgcgatg cacgatgccg atcgagtccg tgccgagcga cgacgtgtgg cgcatcgatt cgccgtgcag ttgccacggc gcgccggaga agatcgcgaa ctgctcgatc gccatcggca tgtcgaagtg catcgattca ctgatcggct tgccattgtt cagcgtatcc atgagcgcgt agtactgctg ccgggccttg aggcggcgca tgatctcgta caggatctcc tgacgcgcgg cgggctgcgt gcgcgcccag cccgggaacg cgcgcgcagc cgcatcgatg gcccgttcgg catccttgcc gtttccggcc gcgatgcaag cgaggacgtc gcccgtcgcc ggattgtgca gatcgatcgt tttgccggta tcgcccggcg tccacttgcc gtcgatgaac tggccatatt cgtgaagaaa gcctgcgccg gcaggggcgt ggaagggggt ggtc
Expected Protein Sequence
MAHHHHHHMT ATTPFHAPAG AGFLHEYGQF IDGKWTPGDT GKTIDLHNPA TGDVLACIAA GNGKDAERAI DAAARAFPGW ARTQPAARQE ILYEIMRRLK ARQQYYALMD TLNNGKPISE SMHFDMPMAI EQFAIFSGAP WQLHGESMRH TSSLGTDSIG IVHREPLGVV VQIIPWNVPM IMLACKIAPA LAAGNTIVLK PSEIVCLSVL EFAREMADLL PPGVLNIVTG YGPDLGEALV TDTRVRKVGF TGSRPTARKL VQYAAANIIP QTMELGGKSA NIVCEDADLD AAAEGAAMST VLNKGEVCLA GSRLFVHEKV RDDFLDKLQR ILAGIRIGDP TSPDTQLGPQ ASRMQMDKIL GYLEEGPREG ARVLTGGGRA RVPGYDNGLF IQPTVFVDVK NSMKIAQEEI FGPVTSVLTW KDDDEVVRLA NASVYGLGGG IWTANLARAH RMACALETGT VWINRYYNFL GGMPIGGYKQ SGFGREFAHE VLNHYTLTKS VIVDLQPGAL GIFGARQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg cgaccacccc cttccacgcc cctgccggcg caggctttct tcacgaatat ggccagttca tcgacggcaa gtggacgccg ggcgataccg gcaaaacgat cgatctgcac aatccggcga cgggcgacgt cctcgcttgc atcgcggccg gaaacggcaa ggatgccgaa cgggccatcg atgcggctgc gcgcgcgttc ccgggctggg cgcgcacgca gcccgccgcg cgtcaggaga tcctgtacga gatcatgcgc cgcctcaagg cccggcagca gtactacgcg ctcatggata cgctgaacaa tggcaagccg atcagtgaat cgatgcactt cgacatgccg atggcgatcg agcagttcgc gatcttctcc ggcgcgccgt ggcaactgca cggcgaatcg atgcgccaca cgtcgtcgct cggcacggac tcgatcggca tcgtgcatcg cgagccgctc ggcgtggtcg tgcagatcat tccgtggaac gtgccgatga tcatgctcgc gtgcaagatc gcgccagccc tcgccgccgg taacacgatc gtgctgaagc cgtccgagat cgtatgcctg tcggtgctcg aattcgctcg cgagatggcg gacctgctgc cgcccggcgt cctgaacatc gtgacgggct acggtccgga tctcggcgag gcgctggtga ccgacacgcg cgtgcgcaag gtcggcttca ccggttcgcg gccgaccgcg cgcaagctcg tccagtacgc tgcggccaac atcatcccgc aaacgatgga actcggcgga aaatcggcca acatcgtctg cgaggatgcc gacctcgacg ccgccgccga aggcgcggcc atgtcgacgg tactgaacaa gggcgaagtc tgcctcgcgg gctcgcgcct gttcgtccac gaaaaggtgc gcgatgattt cctcgacaag ctccagcgca ttctcgccgg cattcggatc ggtgacccga cgtcgccgga cacgcagctc ggcccccagg catcgcggat gcagatggac aaaatcctcg gctatctgga agaaggcccg cgcgaaggcg cccgggtgct gacgggcggt ggccgcgccc gcgtgcccgg ctacgacaac ggactcttca ttcaaccgac cgtgttcgtc gacgtgaaga actcgatgaa gatcgcgcag gaggaaatct tcggccccgt cacgagcgtg ctcacctgga aggacgacga cgaggtcgta cggttggcca atgcatccgt ctacggcctg ggcggcggca tctggaccgc caacctcgcg cgcgcccatc gcatggcctg cgcgctggaa accggcacgg tgtggatcaa ccgctactac aactttctcg gcggcatgcc gatcggcggc tacaagcaaa gcggcttcgg ccgcgaattc gcgcacgagg tgctgaacca ctacacgttg acgaagagcg tgatcgtcga cctgcagccc ggcgcactgg gcatcttcgg cgcacggcaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuceA.00020.h.B1.PW37264
PURIFICATION DATe: 3/21/2013
CONCENTRATION: 27mg/ml
OBSERVED MW: 56kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 110µl
PERCENT IDENTITY: 19
PERCENT COVERAGE: 84
Protocol Notes
notes unavailable
Validated AA Sequence
MGAREVGGPD AAAQAVDGCI GQPYDLVVVL PGEHARDGAE XFLLRDLHRV LHVDEHGRLN EESVVVAGHA GAATARQHPG AFARAFFQIA EDFVHLHPRC LGAELRVRRR RVTDPNAGEN ALELVEEIIX HLFVDEQARA READFALVXX XRHGRAFGGG VXVGILADDV GRFSAEFHRL RDDVGRSVLD ELARGRPRTG EADLAHARVG HQRLAEIRTV ARHDVQDAGR QQVRHLASEF EHRQAYDLGR LQHDRVTGGE GWRDLAREHD HRHVPRNDLH DHAERLAMHD ADRVRAERRR VAHRFAVQLP RRAGEDRELL DRHRHVEVHR FTDRLAIVQR IHERVVLLPG LEAAHDLVQD LLTRGGLRAR PARERARSRI DGPFGILAVS GRDASEDVAR RRIVQIDRFA GIARRPLAVD ELAIFVKKAC AGRGVEGGG
Validated NT Sequence
gcgccgggct gcaggtcgac gatcacgctc ttcgtcaacg tgtagtggtt cagcacctcg tgcgcgaatt cgcggccgaa gccgctttgc ttgtagccgc cgatcggcat gccgccgaga aagttgtagt agcggttgat ccacaccgtg ccggtttcca gcgcgcaggc catgcgatgg gcgcgcgcga ggttggcggt ccagatgccg ccgcccaggc cgtagacgga tgcattggcc aaccgtacga cctcgtcgtc gtccttccag gtgagcacgc tcgtgacggg gccgaanatt tcctcctgcg cgatcttcat cgagttcttc acgtcgacga acacggtcgg ttgaatgaag agtccgttgt cgtagccggg cacgcgggcg cggccaccgc ccgtcagcac ccgggcgcct tcgcgcgggc cttcttccag atagccgagg attttgtcca tctgcatccg cgatgcctgg gggccgagct gcgtgtccgg cgacgtcggg tcaccgatcc naatgccggc gagaatgcgc tggagcttgt cgaggaaatc atcnngcacc ttttcgtgga cgaacaggcg cgagcccgcg aggcagactt cgcccttgtt cantanngtc gacatggccg cgccttcggc ggcggcgtcn aggtcggcat cctcgcagac gatgttggnc gattttccgc cgagttccat cgtttgcggg atgatgttgg ccgcagcgta ctggacgagc ttgcgcgcgg tcggccgcga accggtgaag ccgaccttgc gcacgcgcgt gtcggtcacc agcgcctcgc cgagatccgg accgtagccc gtcacgatgt tcaggacgcc gggcggcagc aggtccgcca tctcgcgagc gaattcgagc accgacaggc atacgatctc ggacggcttc agcacgatcg tgttaccggc ggcgagggct ggcgcgatct tgcacgcgag catgatcatc ggcacgttcc acggaatgat ctgcacgacc acgccgagcg gctcgcgatg cacgatgccg atcgagtccg tgccgagcga cgacgtgtgg cgcatcgatt cgccgtgcag ttgccacggc gcgccggaga agatcgcgaa ctgctcgatc gccatcggca tgtcgaagtg catcgattca ctgatcggct tgccattgtt cagcgtatcc atgagcgcgt agtactgctg ccgggccttg aggcggcgca tgatctcgta caggatctcc tgacgcgcgg cgggctgcgt gcgcgcccag cccgggaacg cgcgcgcagc cgcatcgatg gcccgttcgg catccttgcc gtttccggcc gcgatgcaag cgaggacgtc gcccgtcgcc ggattgtgca gatcgatcgt tttgccggta tcgcccggcg tccacttgcc gtcgatgaac tggccatatt cgtgaagaaa gcctgcgccg gcaggggcgt ggaagggggt ggtc
Expressed Protein Sequence
MAHHHHHHMT ATTPFHAPAG AGFLHEYGQF IDGKWTPGDT GKTIDLHNPA TGDVLACIAA GNGKDAERAI DAAARAFPGW ARTQPAARQE ILYEIMRRLK ARQQYYALMD TLNNGKPISE SMHFDMPMAI EQFAIFSGAP WQLHGESMRH TSSLGTDSIG IVHREPLGVV VQIIPWNVPM IMLACKIAPA LAAGNTIVLK PSEIVCLSVL EFAREMADLL PPGVLNIVTG YGPDLGEALV TDTRVRKVGF TGSRPTARKL VQYAAANIIP QTMELGGKSA NIVCEDADLD AAAEGAAMST VLNKGEVCLA GSRLFVHEKV RDDFLDKLQR ILAGIRIGDP TSPDTQLGPQ ASRMQMDKIL GYLEEGPREG ARVLTGGGRA RVPGYDNGLF IQPTVFVDVK NSMKIAQEEI FGPVTSVLTW KDDDEVVRLA NASVYGLGGG IWTANLARAH RMACALETGT VWINRYYNFL GGMPIGGYKQ SGFGREFAHE VLNHYTLTKS VIVDLQPGAL GIFGARQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCG CGACCACCCC CTTCCACGCC CCTGCCGGCG CAGGCTTTCT TCACGAATAT GGCCAGTTCA TCGACGGCAA GTGGACGCCG GGCGATACCG GCAAAACGAT CGATCTGCAC AATCCGGCGA CGGGCGACGT CCTCGCTTGC ATCGCGGCCG GAAACGGCAA GGATGCCGAA CGGGCCATCG ATGCGGCTGC GCGCGCGTTC CCGGGCTGGG CGCGCACGCA GCCCGCCGCG CGTCAGGAGA TCCTGTACGA GATCATGCGC CGCCTCAAGG CCCGGCAGCA GTACTACGCG CTCATGGATA CGCTGAACAA TGGCAAGCCG ATCAGTGAAT CGATGCACTT CGACATGCCG ATGGCGATCG AGCAGTTCGC GATCTTCTCC GGCGCGCCGT GGCAACTGCA CGGCGAATCG ATGCGCCACA CGTCGTCGCT CGGCACGGAC TCGATCGGCA TCGTGCATCG CGAGCCGCTC GGCGTGGTCG TGCAGATCAT TCCGTGGAAC GTGCCGATGA TCATGCTCGC GTGCAAGATC GCGCCAGCCC TCGCCGCCGG TAACACGATC GTGCTGAAGC CGTCCGAGAT CGTATGCCTG TCGGTGCTCG AATTCGCTCG CGAGATGGCG GACCTGCTGC CGCCCGGCGT CCTGAACATC GTGACGGGCT ACGGTCCGGA TCTCGGCGAG GCGCTGGTGA CCGACACGCG CGTGCGCAAG GTCGGCTTCA CCGGTTCGCG GCCGACCGCG CGCAAGCTCG TCCAGTACGC TGCGGCCAAC ATCATCCCGC AAACGATGGA ACTCGGCGGA AAATCGGCCA ACATCGTCTG CGAGGATGCC GACCTCGACG CCGCCGCCGA AGGCGCGGCC ATGTCGACGG TACTGAACAA GGGCGAAGTC TGCCTCGCGG GCTCGCGCCT GTTCGTCCAC GAAAAGGTGC GCGATGATTT CCTCGACAAG CTCCAGCGCA TTCTCGCCGG CATTCGGATC GGTGACCCGA CGTCGCCGGA CACGCAGCTC GGCCCCCAGG CATCGCGGAT GCAGATGGAC AAAATCCTCG GCTATCTGGA AGAAGGCCCG CGCGAAGGCG CCCGGGTGCT GACGGGCGGT GGCCGCGCCC GCGTGCCCGG CTACGACAAC GGACTCTTCA TTCAACCGAC CGTGTTCGTC GACGTGAAGA ACTCGATGAA GATCGCGCAG GAGGAAATCT TCGGCCCCGT CACGAGCGTG CTCACCTGGA AGGACGACGA CGAGGTCGTA CGGTTGGCCA ATGCATCCGT CTACGGCCTG GGCGGCGGCA TCTGGACCGC CAACCTCGCG CGCGCCCATC GCATGGCCTG CGCGCTGGAA ACCGGCACGG TGTGGATCAA CCGCTACTAC AACTTTCTCG GCGGCATGCC GATCGGCGGC TACAAGCAAA GCGGCTTCGG CCGCGAATTC GCGCACGAGG TGCTGAACCA CTACACGTTG ACGAAGAGCG TGATCGTCGA CCTGCAGCCC GGCGCACTGG GCATCTTCGG CGCACGGCAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT