SSGCID: Target Page

BuceA.00020.f

Putative betaine aldehyde dehydrogenase

CENTER ID:	BuceA.00020.f
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00020.f.B1.GE36437	Full length( BuceA.00020.f )		1	478

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.1902
RefSeq:	YP_002230958.1
UniProt:	B4EAD0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKIYDQFYID GAWRKPAGTG TIDVIDSGTE AVIGRIPEGV ASDAQDAIRA ARAAFDAWAA TPAATRAGYL RKIVEHLQAR SEELAQSITG EVGMPIKLSR AIQVGGPIYN WKAYAKLAES FEFEARVGNS LVVREPVGVV AAITPWNYPL NQITLKVAPA LAAGCTVVLK PSEVAPLNAF MLAEAIHEAG LPAGVFNLVC GYGPVVGEVL ATDPDVDMVS FTGSTRAGKR VAELAAAGVK RVALELGGKS ASVILDDADF ATAVKGTVNA CYLNAGQTCS AHTRMLVPEA RYDEAREIAK AVAETYVAGD PRQEATRLGA LASAVQQQRV QAYIQRGIDE GAELVTGGTG LPEGLAKGFF VKPTVFGRVD PKSTIAQEEI FGPVLSIITY RDEDEAVRIA NDSPYGLGGA VWAGSDERAM RVARRIRTGQ VDINGGTWNG AAPFGGFKQS GHGRENGVYG LEEYLEYKSM QLKPAKPA

NT Sequence

ATGAAAATCT ACGACCAGTT CTACATCGAC GGCGCATGGC GCAAACCGGC CGGCACCGGC ACGATCGACG TGATCGACTC GGGCACCGAA GCCGTGATCG GCCGGATTCC CGAGGGCGTC GCGTCCGACG CACAGGACGC GATCCGCGCG GCGCGCGCGG CCTTCGACGC GTGGGCGGCC ACGCCGGCCG CGACGCGCGC GGGTTATCTG CGCAAGATCG TCGAGCATCT GCAGGCGCGC AGCGAGGAAC TCGCGCAGTC GATCACCGGC GAAGTCGGCA TGCCGATCAA GCTGTCGCGC GCGATCCAGG TCGGCGGCCC GATCTACAAC TGGAAGGCGT ACGCGAAGCT CGCCGAATCG TTCGAATTCG AGGCCCGGGT CGGCAATTCG CTGGTGGTGC GCGAGCCGGT CGGCGTCGTC GCGGCGATCA CGCCGTGGAA CTACCCGCTC AACCAGATCA CGCTGAAGGT CGCGCCGGCG CTCGCGGCCG GCTGCACGGT GGTGCTGAAG CCGTCCGAAG TCGCGCCGCT CAACGCGTTC ATGCTCGCCG AAGCGATCCA CGAGGCCGGG CTGCCGGCCG GCGTGTTCAA CCTCGTGTGC GGGTATGGCC CGGTCGTCGG CGAGGTGTTG GCGACCGACC CGGACGTCGA CATGGTGTCG TTCACCGGCT CGACGCGCGC GGGCAAGCGC GTGGCCGAAC TGGCGGCCGC GGGCGTCAAG CGCGTCGCGC TGGAGCTCGG CGGCAAGTCG GCATCGGTGA TCCTCGACGA CGCCGATTTC GCGACGGCGG TGAAGGGCAC CGTCAACGCG TGCTACCTGA ACGCCGGCCA GACCTGCTCC GCGCATACGC GGATGCTGGT GCCGGAAGCT CGCTACGACG AAGCGCGCGA GATCGCGAAG GCCGTGGCCG AAACGTACGT CGCGGGCGAC CCGCGGCAGG AGGCGACGCG CCTCGGCGCG CTGGCGTCGG CCGTGCAGCA GCAGCGCGTG CAGGCCTACA TCCAGCGCGG GATCGACGAA GGCGCGGAAC TCGTGACGGG CGGCACCGGG TTGCCGGAAG GGCTCGCGAA GGGCTTCTTC GTGAAGCCGA CCGTGTTCGG CCGCGTCGAT CCGAAATCGA CGATCGCGCA GGAAGAGATC TTCGGGCCGG TGCTGTCGAT CATCACGTAT CGCGATGAAG ACGAAGCCGT GCGGATCGCG AACGATTCGC CGTACGGGCT CGGCGGCGCG GTGTGGGCCG GCAGCGACGA ACGCGCGATG CGCGTTGCGC GCCGCATCCG CACCGGGCAG GTCGACATCA ACGGCGGCAC GTGGAACGGC GCCGCGCCGT TCGGCGGCTT CAAGCAGTCC GGGCACGGCC GCGAGAACGG CGTGTACGGG CTCGAAGAGT ATCTCGAATA CAAGTCGATG CAGTTGAAGC CGGCCAAGCC GGCC

Details for BuceA.00020.f.B1.GE36437

HARVESTED ON:	11/21/2012
SEQUENCED ON:	11/27/2012
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	24
PERCENT COVERAGE:	78

Validated AA Sequence

MAQTGRHRHD RRDRLGHRSR DRPDSRGRRV RRTGRDPRGA RGLRRVGGHA GRDARGLSAQ DRRASAGAQR GTRAVDHRRS RHADQAVARD PGRRPDLQLE GVREARRIVR IRGPGRQFAG GARAGRRRRG DHAVELPAQP DHAEGRAGAR GRLHGGAEAV RSRAAQRVHA RRSDPRGRAA GRRVQPRVRV WPGRRRGVGD RPGRRHGVVH RLDARGQARG RTGGRGRQAR RAGARRQVGI GDPRRRRFRD GGEGHRQRVL PERRPDLLRA YADAGAGSSL RRSARDREGR GRNVRRGRPA AGGDAPRRAG VGRAAAARAG LHPARDRRRR GTRDGRHRVA GRAREGLLRE ADRVRPRRSE IDDRAGRDLR AGAVDHHVSR

Validated NT Sequence

atcgacttgt attcgagata ctcttcgagc ccgtacacgc cgttctcgcg gccgtgcccg gactgcttga agccgccgaa cggcgcggcg ccgttccacg tgccgccgtt gatgtcgacc tgcccggtgc ggatgcggcg cgcaacgcgc atcgcgcgtt cgtcgctgcc ggcccacacc gcgccgccga gcccgtacgg cgaatcgttc gcgatccgca cggcttcgtc ttcatcgcga tacgtgatga tcgacagcac cggcccgaag atctcttcct gcgcgatcgt cgatttcgga tcgacgcggc cgaacacggt cggcttcacg aagaagccct tcgcgagccc ttccggcaac ccggtgccgc ccgtcacgag ttccgcgcct tcgtcgatcc cgcgctggat gtaggcctgc acgcgctgct gctgcacggc cgacgccagc gcgccgaggc gcgtcgcctc ctgccgcggg tcgcccgcga cgtacgtttc ggccacggcc ttcgcgatct cgcgcgcttc gtcgtagcga gcttccggca ccagcatccg cgtatgcgcg gagcaggtct ggccggcgtt caggtagcac gcgttgacgg tgcccttcac cgccgtcgcg aaatcggcgt cgtcgaggat caccgatgcc gacttgccgc cgagctccag cgcgacgcgc ttgacgcccg cggccgccag ttcggccacg cgcttgcccg cgcgcgtcga gccggtgaac gacaccatgt cgacgtccgg gtcggtcgcc aacacctcgc cgacgaccgg gccatacccg cacacgaggt tgaacacgcc ggccggcagc ccggcctcgt ggatcgcttc ggcgagcatg aacgcgttga gcggcgcgac ttcggacggc ttcagcacca ccgtgcagcc ggccgcgagc gccggcgcga ccttcagcgt gatctggttg agcgggtagt tccacggcgt gatcgccgcg acgacgccga ccggctcgcg caccaccagc gaattgccga cccgggcctc gaattcgaac gattcggcga gcttcgcgta cgccttccag ttgtagatcg ggccgccgac ctggatcgcg cgcgacagct tgatcggcat gccgacttcg ccggtgatcg actgcgcgag ttcctcgctg cgcgcctgca gatgctcgac gatcttgcgc agataacccg cgcgcgtcgc ggccggcgtg gccgcccacg cgtcgaaggc cgcgcgcgcc gcgcggatcg cgtcctgtgc gtcggacgcg acgccctcgg gaatccggcc gatcacggct tcggtgcccg agtcgatcac gtcgatcgtg ccggtgccgg ccggtttgcg ccatgcgccg tcgatgtaga actggtcgta g

Expected Protein Sequence

MAHHHHHHMK IYDQFYIDGA WRKPAGTGTI DVIDSGTEAV IGRIPEGVAS DAQDAIRAAR AAFDAWAATP AATRAGYLRK IVEHLQARSE ELAQSITGEV GMPIKLSRAI QVGGPIYNWK AYAKLAESFE FEARVGNSLV VREPVGVVAA ITPWNYPLNQ ITLKVAPALA AGCTVVLKPS EVAPLNAFML AEAIHEAGLP AGVFNLVCGY GPVVGEVLAT DPDVDMVSFT GSTRAGKRVA ELAAAGVKRV ALELGGKSAS VILDDADFAT AVKGTVNACY LNAGQTCSAH TRMLVPEARY DEAREIAKAV AETYVAGDPR QEATRLGALA SAVQQQRVQA YIQRGIDEGA ELVTGGTGLP EGLAKGFFVK PTVFGRVDPK STIAQEEIFG PVLSIITYRD EDEAVRIAND SPYGLGGAVW AGSDERAMRV ARRIRTGQVD INGGTWNGAA PFGGFKQSGH GRENGVYGLE EYLEYKSMQL KPAKPA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa tctacgacca gttctacatc gacggcgcat ggcgcaaacc ggccggcacc ggcacgatcg acgtgatcga ctcgggcacc gaagccgtga tcggccggat tcccgagggc gtcgcgtccg acgcacagga cgcgatccgc gcggcgcgcg cggccttcga cgcgtgggcg gccacgccgg ccgcgacgcg cgcgggttat ctgcgcaaga tcgtcgagca tctgcaggcg cgcagcgagg aactcgcgca gtcgatcacc ggcgaagtcg gcatgccgat caagctgtcg cgcgcgatcc aggtcggcgg cccgatctac aactggaagg cgtacgcgaa gctcgccgaa tcgttcgaat tcgaggcccg ggtcggcaat tcgctggtgg tgcgcgagcc ggtcggcgtc gtcgcggcga tcacgccgtg gaactacccg ctcaaccaga tcacgctgaa ggtcgcgccg gcgctcgcgg ccggctgcac ggtggtgctg aagccgtccg aagtcgcgcc gctcaacgcg ttcatgctcg ccgaagcgat ccacgaggcc gggctgccgg ccggcgtgtt caacctcgtg tgcgggtatg gcccggtcgt cggcgaggtg ttggcgaccg acccggacgt cgacatggtg tcgttcaccg gctcgacgcg cgcgggcaag cgcgtggccg aactggcggc cgcgggcgtc aagcgcgtcg cgctggagct cggcggcaag tcggcatcgg tgatcctcga cgacgccgat ttcgcgacgg cggtgaaggg caccgtcaac gcgtgctacc tgaacgccgg ccagacctgc tccgcgcata cgcggatgct ggtgccggaa gctcgctacg acgaagcgcg cgagatcgcg aaggccgtgg ccgaaacgta cgtcgcgggc gacccgcggc aggaggcgac gcgcctcggc gcgctggcgt cggccgtgca gcagcagcgc gtgcaggcct acatccagcg cgggatcgac gaaggcgcgg aactcgtgac gggcggcacc gggttgccgg aagggctcgc gaagggcttc ttcgtgaagc cgaccgtgtt cggccgcgtc gatccgaaat cgacgatcgc gcaggaagag atcttcgggc cggtgctgtc gatcatcacg tatcgcgatg aagacgaagc cgtgcggatc gcgaacgatt cgccgtacgg gctcggcggc gcggtgtggg ccggcagcga cgaacgcgcg atgcgcgttg cgcgccgcat ccgcaccggg caggtcgaca tcaacggcgg cacgtggaac ggcgccgcgc cgttcggcgg cttcaagcag tccgggcacg gccgcgagaa cggcgtgtac gggctcgaag agtatctcga atacaagtcg atgcagttga agccggccaa gccggcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at