SSGCID: Target Page

BuceA.00020.e

Aldehyde dehydrogenase family protein

CENTER ID:	BuceA.00020.e
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00020.e.B1.GE36436	Full length( BuceA.00020.e )		1	483

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00020.e.B1.PW37273	Full length( BuceA.00020.e )		1	483

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2894
RefSeq:	YP_002231897.1
UniProt:	B4E9J4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQTVAMLIGG ERRHSSGGAT FVRRNPLDGQ VASEAPACTV DDARAAADAA ARAFPDWAAL GPGARRALLL KAAAALEAKH DQFVAAMAAE TGASALWAGF NVHLAASGLV EAASLTTQVG GELIPSDVPG SLAMGVRQPA GVVLGIAPWN APVILATRAL ALPLACGNTV VLKGSELCPV THGLIVEALQ EAGLPPGVVN FVTNAPDDAG AVVDALIAHP AVRRVNFTGS TRVGRLVAQA CARHLKPSVL ELGGKAPFVV LDDADLDAAV AAAAFGAFAN SGQICMSTER IVVDAAIADA FVSKLADKAA SLPLGDPRNG PVVLGSLIDT KAVERCNALI DDALAHGATL LCGGKADTTL FPATLLDHVT PAMRIYAEES FGPVKGIVRV DGEEAAIRCA NDNAFGLASA VFSRDVARAL RVAARIESGI CHVNGPTVHD EAQMPFGGVK DSGFGHFGGK AGIAAFTDLR WITVQTAPRH YPF

NT Sequence

ATGCAAACCG TAGCGATGCT GATCGGCGGC GAACGCCGCC ACTCGTCCGG CGGCGCGACC TTCGTGCGCC GCAACCCGCT CGACGGCCAA GTCGCGTCCG AAGCGCCCGC ATGCACCGTC GACGATGCGC GCGCGGCGGC CGATGCGGCT GCCCGTGCAT TTCCCGACTG GGCCGCGCTC GGGCCCGGCG CGCGGCGCGC CTTGCTGCTG AAGGCGGCCG CCGCGCTCGA AGCGAAGCAC GACCAGTTCG TTGCGGCGAT GGCGGCCGAG ACGGGGGCGT CCGCGCTGTG GGCCGGCTTC AACGTTCACC TCGCGGCGAG CGGGCTCGTC GAGGCCGCGT CGCTCACCAC GCAGGTCGGC GGCGAGCTGA TTCCGTCCGA CGTGCCGGGC TCGCTCGCGA TGGGCGTGCG GCAACCGGCC GGCGTCGTGC TCGGCATCGC GCCGTGGAAC GCGCCGGTGA TCCTCGCGAC GCGCGCGCTC GCGCTGCCGC TCGCGTGCGG CAACACGGTC GTGCTGAAGG GCTCGGAGCT GTGTCCCGTC ACGCACGGGC TGATCGTCGA GGCGCTGCAG GAGGCCGGAC TGCCGCCGGG CGTCGTCAAC TTCGTGACGA ACGCGCCCGA CGATGCGGGC GCCGTCGTCG ATGCGCTGAT CGCGCATCCG GCCGTGCGCC GCGTGAATTT CACCGGCTCG ACGCGGGTCG GGCGGCTCGT CGCGCAAGCG TGCGCGCGTC ACCTGAAGCC GTCGGTGCTG GAACTCGGCG GCAAGGCGCC GTTCGTCGTG CTCGACGATG CCGATCTCGA CGCGGCGGTC GCCGCGGCCG CATTCGGCGC ATTCGCGAAT TCCGGGCAGA TCTGCATGTC GACCGAGCGC ATCGTCGTCG ACGCGGCGAT CGCCGATGCG TTCGTCTCGA AGCTCGCCGA CAAGGCCGCG TCGCTGCCGC TCGGCGATCC GCGCAACGGG CCCGTCGTGC TCGGTTCGCT GATCGACACG AAGGCGGTCG AGCGCTGCAA CGCGCTGATC GACGATGCGC TCGCGCACGG CGCGACGCTG CTGTGCGGCG GCAAGGCCGA CACCACGCTG TTCCCGGCGA CGCTGCTCGA CCATGTGACG CCGGCGATGC GCATCTACGC GGAAGAATCG TTCGGCCCGG TGAAGGGCAT CGTGCGCGTC GACGGCGAGG AGGCCGCGAT CCGTTGCGCG AACGACAACG CGTTCGGGCT GGCGTCGGCC GTGTTCAGCC GCGACGTCGC GCGGGCGCTG CGCGTCGCGG CGCGCATCGA ATCGGGCATC TGTCACGTGA ACGGCCCGAC CGTGCACGAC GAGGCGCAGA TGCCGTTCGG CGGCGTGAAG GACAGCGGTT TCGGCCACTT CGGCGGCAAG GCAGGCATCG CCGCGTTCAC CGACCTGCGC TGGATCACCG TGCAGACGGC GCCGCGCCAC TATCCGTTC

Details for BuceA.00020.e.B1.GE36436

HARVESTED ON:	11/21/2012
SEQUENCED ON:	11/27/2012
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

MLIGGERRHS SGGATFVRRN PLDGQVASEA PACTVDDARA AADAAARAFP DWAALGPGAR RALLLKAAAA LEAKHDQFVA AMAAETGASA LWAGFNVHLA ASGLVEAASL TTQVGGELIP SDVPGSLAMG VRQPAGVVLG IAPWNAPVIL ATRALALPLA CGNTVVLKGS ELCPVTHGLI VEALQEAGLP PGVVNFVTNA PDDAGAVVDA LIAHPAVRRV NFTGSTRVGR LVAQACARHL KPSVLELGGK APFVVLDDAD LDAAVAAAAF GAFANSGQIC MSTERIVVDA AIADAFVSKL ADKAASLPLG DPRNGPVVLG SLIDTKAVER CNALIDDALA HGATLLCGGK ADTTLFPATL LDHVTPAMRI YAEESFGPVK GIVRVDGEEA AIRCANDNAF GLASAVFSRD VARALRVAAR IESGICHVNG PTVHDEAQMP FGGVKDSGFG HFGGKAGIAA FTDLRWITVQ

Validated NT Sequence

tctgcacggt gatccagcgc aggtcggtga acgcggcgat gcctgccttg ccgccgaagt ggccgaaacc gctgtccttc acgccgccga acggcatctg cgcctcgtcg tgcacggtcg ggccgttcac gtgacagatg cccgattcga tgcgcgccgc gacgcgcagc gcccgcgcga cgtcgcggct gaacacggcc gacgccagcc cgaacgcgtt gtcgttcgcg caacggatcg cggcctcctc gccgtcgacg cgcacgatgc ccttcaccgg gccgaacgat tcttccgcgt agatgcgcat cgccggcgtc acatggtcga gcagcgtcgc cgggaacagc gtggtgtcgg ccttgccgcc gcacagcagc gtcgcgccgt gcgcgagcgc atcgtcgatc agcgcgttgc agcgctcgac cgccttcgtg tcgatcagcg aaccgagcac gacgggcccg ttgcgcggat cgccgagcgg cagcgacgcg gccttgtcgg cgagcttcga gacgaacgca tcggcgatcg ccgcgtcgac gacgatgcgc tcggtcgaca tgcagatctg cccggaattc gcgaatgcgc cgaatgcggc cgcggcgacc gccgcgtcga gatcggcatc gtcgagcacg acgaacggcg ccttgccgcc gagttccagc accgacggct tcaggtgacg cgcgcacgct tgcgcgacga gccgcccgac ccgcgtcgag ccggtgaaat tcacgcggcg cacggccgga tgcgcgatca gcgcatcgac gacggcgccc gcatcgtcgg gcgcgttcgt cacgaagttg acgacgcccg gcggcagtcc ggcctcctgc agcgcctcga cgatcagccc gtgcgtgacg ggacacagct ccgagccctt cagcacgacc gtgttgccgc acgcgagcgg cagcgcgagc gcgcgcgtcg cgaggatcac cggcgcgttc cacggcgcga tgccgagcac gacgccggcc ggttgccgca cgcccatcgc gagcgagccc ggcacgtcgg acggaatcag ctcgccgccg acctgcgtgg tgagcgacgc ggcctcgacg agcccgctcg ccgcgaggtg aacgttgaag ccggcccaca gcgcggacgc ccccgtctcg gccgccatcg ccgcaacgaa ctggtcgtgc ttcgcttcga gcgcggcggc cgccttcagc agcaaggcgc gccgcgcgcc gggcccgagc gcggcccagt cgggaaatgc acgggcagcc gcatcggccg ccgcgcgcgc atcgtcgacg gtgcatgcgg gcgcttcgga cgcgacttgg ccgtcgagcg ggttgcggcg cacgaaggtc gcgccgccgg acgagtggcg gcgttcgccg ccgatcagca tcgctacgg

Expected Protein Sequence

MAHHHHHHMQ TVAMLIGGER RHSSGGATFV RRNPLDGQVA SEAPACTVDD ARAAADAAAR AFPDWAALGP GARRALLLKA AAALEAKHDQ FVAAMAAETG ASALWAGFNV HLAASGLVEA ASLTTQVGGE LIPSDVPGSL AMGVRQPAGV VLGIAPWNAP VILATRALAL PLACGNTVVL KGSELCPVTH GLIVEALQEA GLPPGVVNFV TNAPDDAGAV VDALIAHPAV RRVNFTGSTR VGRLVAQACA RHLKPSVLEL GGKAPFVVLD DADLDAAVAA AAFGAFANSG QICMSTERIV VDAAIADAFV SKLADKAASL PLGDPRNGPV VLGSLIDTKA VERCNALIDD ALAHGATLLC GGKADTTLFP ATLLDHVTPA MRIYAEESFG PVKGIVRVDG EEAAIRCAND NAFGLASAVF SRDVARALRV AARIESGICH VNGPTVHDEA QMPFGGVKDS GFGHFGGKAG IAAFTDLRWI TVQTAPRHYP F

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa ccgtagcgat gctgatcggc ggcgaacgcc gccactcgtc cggcggcgcg accttcgtgc gccgcaaccc gctcgacggc caagtcgcgt ccgaagcgcc cgcatgcacc gtcgacgatg cgcgcgcggc ggccgatgcg gctgcccgtg catttcccga ctgggccgcg ctcgggcccg gcgcgcggcg cgccttgctg ctgaaggcgg ccgccgcgct cgaagcgaag cacgaccagt tcgttgcggc gatggcggcc gagacggggg cgtccgcgct gtgggccggc ttcaacgttc acctcgcggc gagcgggctc gtcgaggccg cgtcgctcac cacgcaggtc ggcggcgagc tgattccgtc cgacgtgccg ggctcgctcg cgatgggcgt gcggcaaccg gccggcgtcg tgctcggcat cgcgccgtgg aacgcgccgg tgatcctcgc gacgcgcgcg ctcgcgctgc cgctcgcgtg cggcaacacg gtcgtgctga agggctcgga gctgtgtccc gtcacgcacg ggctgatcgt cgaggcgctg caggaggccg gactgccgcc gggcgtcgtc aacttcgtga cgaacgcgcc cgacgatgcg ggcgccgtcg tcgatgcgct gatcgcgcat ccggccgtgc gccgcgtgaa tttcaccggc tcgacgcggg tcgggcggct cgtcgcgcaa gcgtgcgcgc gtcacctgaa gccgtcggtg ctggaactcg gcggcaaggc gccgttcgtc gtgctcgacg atgccgatct cgacgcggcg gtcgccgcgg ccgcattcgg cgcattcgcg aattccgggc agatctgcat gtcgaccgag cgcatcgtcg tcgacgcggc gatcgccgat gcgttcgtct cgaagctcgc cgacaaggcc gcgtcgctgc cgctcggcga tccgcgcaac gggcccgtcg tgctcggttc gctgatcgac acgaaggcgg tcgagcgctg caacgcgctg atcgacgatg cgctcgcgca cggcgcgacg ctgctgtgcg gcggcaaggc cgacaccacg ctgttcccgg cgacgctgct cgaccatgtg acgccggcga tgcgcatcta cgcggaagaa tcgttcggcc cggtgaaggg catcgtgcgc gtcgacggcg aggaggccgc gatccgttgc gcgaacgaca acgcgttcgg gctggcgtcg gccgtgttca gccgcgacgt cgcgcgggcg ctgcgcgtcg cggcgcgcat cgaatcgggc atctgtcacg tgaacggccc gaccgtgcac gacgaggcgc agatgccgtt cggcggcgtg aaggacagcg gtttcggcca cttcggcggc aaggcaggca tcgccgcgtt caccgacctg cgctggatca ccgtgcagac ggcgccgcgc cactatccgt tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for BuceA.00020.e.B1.PW37273

PURIFICATION DATe:	4/1/2013
CONCENTRATION:	40.4mg/ml
OBSERVED MW:	49kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	18
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAA CCGTAGCGAT GCTGATCGGC GGCGAACGCC GCCACTCGTC CGGCGGCGCG ACCTTCGTGC GCCGCAACCC GCTCGACGGC CAAGTCGCGT CCGAAGCGCC CGCATGCACC GTCGACGATG CGCGCGCGGC GGCCGATGCG GCTGCCCGTG CATTTCCCGA CTGGGCCGCG CTCGGGCCCG GCGCGCGGCG CGCCTTGCTG CTGAAGGCGG CCGCCGCGCT CGAAGCGAAG CACGACCAGT TCGTTGCGGC GATGGCGGCC GAGACGGGGG CGTCCGCGCT GTGGGCCGGC TTCAACGTTC ACCTCGCGGC GAGCGGGCTC GTCGAGGCCG CGTCGCTCAC CACGCAGGTC GGCGGCGAGC TGATTCCGTC CGACGTGCCG GGCTCGCTCG CGATGGGCGT GCGGCAACCG GCCGGCGTCG TGCTCGGCAT CGCGCCGTGG AACGCGCCGG TGATCCTCGC GACGCGCGCG CTCGCGCTGC CGCTCGCGTG CGGCAACACG GTCGTGCTGA AGGGCTCGGA GCTGTGTCCC GTCACGCACG GGCTGATCGT CGAGGCGCTG CAGGAGGCCG GACTGCCGCC GGGCGTCGTC AACTTCGTGA CGAACGCGCC CGACGATGCG GGCGCCGTCG TCGATGCGCT GATCGCGCAT CCGGCCGTGC GCCGCGTGAA TTTCACCGGC TCGACGCGGG TCGGGCGGCT CGTCGCGCAA GCGTGCGCGC GTCACCTGAA GCCGTCGGTG CTGGAACTCG GCGGCAAGGC GCCGTTCGTC GTGCTCGACG ATGCCGATCT CGACGCGGCG GTCGCCGCGG CCGCATTCGG CGCATTCGCG AATTCCGGGC AGATCTGCAT GTCGACCGAG CGCATCGTCG TCGACGCGGC GATCGCCGAT GCGTTCGTCT CGAAGCTCGC CGACAAGGCC GCGTCGCTGC CGCTCGGCGA TCCGCGCAAC GGGCCCGTCG TGCTCGGTTC GCTGATCGAC ACGAAGGCGG TCGAGCGCTG CAACGCGCTG ATCGACGATG CGCTCGCGCA CGGCGCGACG CTGCTGTGCG GCGGCAAGGC CGACACCACG CTGTTCCCGG CGACGCTGCT CGACCATGTG ACGCCGGCGA TGCGCATCTA CGCGGAAGAA TCGTTCGGCC CGGTGAAGGG CATCGTGCGC GTCGACGGCG AGGAGGCCGC GATCCGTTGC GCGAACGACA ACGCGTTCGG GCTGGCGTCG GCCGTGTTCA GCCGCGACGT CGCGCGGGCG CTGCGCGTCG CGGCGCGCAT CGAATCGGGC ATCTGTCACG TGAACGGCCC GACCGTGCAC GACGAGGCGC AGATGCCGTT CGGCGGCGTG AAGGACAGCG GTTTCGGCCA CTTCGGCGGC AAGGCAGGCA TCGCCGCGTT CACCGACCTG CGCTGGATCA CCGTGCAGAC GGCGCCGCGC CACTATCCGT TCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT