BuceA.00020.c
Putative aldehyde dehydrogenase
| CENTER ID: | BuceA.00020.c |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00020.c.B1.GE36434 | Full length( BuceA.00020.c ) | 1 | 479 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00020.c.B1.PS01773 | Full length( BuceA.00020.c ) | 1 | 479 |
Structures
4YWE
DEPOSITED: 3/20/2015
DETERMINATION: XRay
CLONE: BuceA.00020.c.B1.GE36434
PROTEIN: BuceA.00020.c.B1.PS01773
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|216591.5.peg.2715 |
| RefSeq: | YP_002231727.1 |
| UniProt: | B4E8B7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEEAKHFIAG EWTLPAQLET IPVVDPSDGQ PFATIARGTA PDIERAVAAA RDAFAGPWGA ASAAERGRVL MRLSARVTDS IEELAAIEAR DTGKPLKQAR ADAAALARYF EFYAGAADKL HGETLPYQAG YTVLTVREPH GVTGHIVPWN YPMQIFGRSV GAALAAGNAC VVKPAEDACL SVLRVAELAA EAGLPAGALN IVTGYGHEAG AALARHPGID HISFTGSPAT GKLVTQMAAE NHVPVTLELG GKSPQIVFAD ADLDAALPVL VSAIVQNGGQ TCSAGSRVLI ERAVYEPLVE RLATAFNGLR VGPSRADLDC GPLINAKQQQ RVWDFLSDAQ HDGIPMAAHG QVVADAPESG FYQAPALLRD VPPSHRLAQE EVFGPVLAAM RFVDEDEAVA LANGTPYGLV AGIWTRDGAR QMRLARRLRA GQVFINNYGA GGGVELPFGG VGHSGHGREK GFEALYGFTA LKTIAIRHG
NT Sequence
ATGGAAGAAG CGAAGCACTT CATCGCGGGC GAATGGACGT TGCCCGCGCA ACTGGAAACC ATCCCGGTCG TCGATCCGTC CGACGGCCAG CCGTTCGCGA CGATCGCGCG CGGCACCGCG CCCGACATCG AGCGCGCGGT CGCCGCGGCC CGCGACGCAT TCGCAGGCCC ATGGGGCGCC GCGAGCGCCG CCGAGCGCGG CCGCGTGCTG ATGCGGCTGT CGGCGCGCGT GACCGATTCG ATCGAGGAAC TCGCCGCGAT CGAGGCGCGC GATACCGGCA AGCCGCTGAA GCAGGCGCGC GCCGATGCAG CCGCGCTCGC ACGCTACTTC GAGTTCTACG CGGGCGCGGC CGACAAGCTG CACGGCGAAA CCCTCCCCTA CCAGGCCGGC TACACGGTGC TGACGGTGCG CGAACCGCAC GGCGTCACGG GCCACATCGT GCCGTGGAAC TACCCGATGC AGATCTTCGG GCGCAGCGTC GGCGCGGCGC TCGCGGCCGG CAACGCGTGC GTGGTCAAGC CGGCCGAGGA CGCGTGCCTG TCCGTGCTGC GCGTCGCCGA ACTGGCCGCC GAGGCCGGGC TGCCGGCCGG CGCGCTCAAC ATCGTCACCG GCTACGGCCA TGAAGCGGGC GCCGCGCTCG CCCGCCATCC GGGCATCGAC CACATCTCGT TTACGGGTTC GCCGGCCACC GGCAAGCTGG TCACGCAGAT GGCCGCCGAG AACCACGTGC CGGTCACGCT CGAACTCGGT GGCAAGTCGC CGCAAATCGT GTTCGCCGAT GCCGACCTCG ACGCCGCGCT GCCCGTGCTC GTGTCCGCGA TCGTGCAGAA CGGCGGGCAG ACCTGCTCGG CCGGCAGCCG CGTGCTGATC GAGCGCGCGG TGTACGAGCC GCTCGTCGAG CGTCTCGCCA CCGCGTTCAA CGGGCTGCGC GTCGGCCCGA GCCGCGCCGA TCTCGATTGC GGCCCGCTGA TCAACGCGAA GCAGCAGCAA CGCGTGTGGG ATTTCCTGTC CGACGCGCAG CACGACGGCA TTCCGATGGC CGCGCACGGG CAGGTCGTCG CCGATGCGCC CGAAAGCGGC TTCTATCAGG CGCCCGCGCT GTTGCGCGAC GTGCCGCCGT CGCACCGGCT CGCGCAGGAG GAAGTGTTCG GCCCGGTGCT CGCGGCGATG CGTTTCGTCG ACGAGGACGA AGCCGTCGCG CTCGCGAACG GCACGCCGTA CGGCCTCGTC GCGGGCATCT GGACGCGCGA CGGCGCGCGC CAGATGCGTC TTGCGCGGCG CCTGCGCGCG GGTCAGGTGT TCATCAACAA CTATGGCGCG GGCGGCGGCG TCGAGCTGCC GTTCGGCGGC GTCGGCCATT CGGGCCATGG CCGCGAGAAG GGCTTCGAGG CGCTGTACGG CTTCACCGCG CTGAAGACGA TCGCGATCCG GCACGGC
Details for BuceA.00020.c.B1.GE36434
| HARVESTED ON: | 11/21/2012 |
| SEQUENCED ON: | 11/27/2012 |
| EXPECTED MW: | 51kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 25 |
| PERCENT COVERAGE: | 93 |
Validated AA Sequence
MARMADAAER QLDAAARAIV VDEHLTRAQA PRKTHLARAV ARPDARDEAV RRAVRERDGF VLVDETHRRE HRAEHFLLRE PVRRRHVAQQ RGRLIEAAFG RIGDDLPVRG HRNAVVLRVG QEIPHALLLL XVXXRAAIEI GAARADAQPV ERGGETLDER LXHRALDQHA AAGRAGLPAV LHDRGHEHGQ RGVEVGIGEH DLRRLATEFE RDRHVVLGGH LRDQLAGGRR TRKRDVVDAR MAGERGARFM AVAGDDVERA GRQPGLGGQF GDAQHGQARV LGRLDHARVA GRERRADAAP EDLHRVVPRH DVARDAVRFA HRQHRVAGLV GEGFAVQLVG RARVELEVAC ERGCIGARLL QRLAGIARLD RGEFLDRIGH ARRQPHQHAA ALGGARGAPW ACECVAGRGD RALDVGRGAA RDRRERLAVG RIDDRDGFQL RGQRPFARDE VLRF
Validated NT Sequence
cagcgcggtg aagccgtaca gcgcctcgaa gcccttctcg cggccatggc ccgaatggcc gacgccgccg aacggcagct cgacgccgcc gcccgcgcca tagttgttga tgaacacctg acccgcgcgc aggcgccgcg caagacgcat ctggcgcgcg ccgtcgcgcg tccagatgcc cgcgacgagg ccgtacggcg tgccgttcgc gagcgcgacg gcttcgtcct cgtcgacgaa acgcatcgcc gcgagcaccg ggccgaacac ttcctcctgc gcgagccggt gcgacggcgg cacgtcgcgc aacagcgcgg gcgcctgata gaagccgctt tcgggcgcat cggcgacgac ctgcccgtgc gcggccatcg gaatgccgtc gtgctgcgcg tcggacagga aatcccacac gcgttgctgc tgcttcnngt tnatcancgg gccgcaatcg agatcggcgc ggctcgggcc gacgcgcagc ccgttgaacg cggtggcgag acgctcgacg agcggctcgn acaccgcgcg ctcgatcagc acgcggctgc cggccgagca ggtctgcccg ccgttctgca cgatcgcgga cacgagcacg ggcagcgcgg cgtcgaggtc ggcatcggcg aacacgattt gcggcgactt gccaccgagt tcgagcgtga ccggcacgtg gttctcggcg gccatctgcg tgaccagctt gccggtggcc ggcgaacccg taaacgagat gtggtcgatg cccggatggc gggcgagcgc ggcgcccgct tcatggccgt agccggtgac gatgttgagc gcgccggccg gcagcccggc ctcggcggcc agttcggcga cgcgcagcac ggacaggcac gcgtcctcgg ccggcttgac cacgcacgcg ttgccggccg cgagcgccgc gccgacgctg cgcccgaaga tctgcatcgg gtagttccac ggcacgatgt ggcccgtgac gccgtgcggt tcgcgcaccg tcagcaccgt gtagccggcc tggtagggga gggtttcgcc gtgcagcttg tcggccgcgc ccgcgtagaa ctcgaagtag cgtgcgagcg cggctgcatc ggcgcgcgcc tgcttcagcg gcttgccggt atcgcgcgcc tcgatcgcgg cgagttcctc gatcgaatcg gtcacgcgcg ccgacagccg catcagcacg cggccgcgct cggcggcgct cgcggcgccc catgggcctg cgaatgcgtc gcgggccgcg gcgaccgcgc gctcgatgtc gggcgcggtg ccgcgcgcga tcgtcgcgaa cggctggccg tcggacggat cgacgaccgg gatggtttcc agttgcgcgg gcaacgtcca ttcgcccgcg atgaagtgct tcgcttctt
Expected Protein Sequence
MAHHHHHHME EAKHFIAGEW TLPAQLETIP VVDPSDGQPF ATIARGTAPD IERAVAAARD AFAGPWGAAS AAERGRVLMR LSARVTDSIE ELAAIEARDT GKPLKQARAD AAALARYFEF YAGAADKLHG ETLPYQAGYT VLTVREPHGV TGHIVPWNYP MQIFGRSVGA ALAAGNACVV KPAEDACLSV LRVAELAAEA GLPAGALNIV TGYGHEAGAA LARHPGIDHI SFTGSPATGK LVTQMAAENH VPVTLELGGK SPQIVFADAD LDAALPVLVS AIVQNGGQTC SAGSRVLIER AVYEPLVERL ATAFNGLRVG PSRADLDCGP LINAKQQQRV WDFLSDAQHD GIPMAAHGQV VADAPESGFY QAPALLRDVP PSHRLAQEEV FGPVLAAMRF VDEDEAVALA NGTPYGLVAG IWTRDGARQM RLARRLRAGQ VFINNYGAGG GVELPFGGVG HSGHGREKGF EALYGFTALK TIAIRHG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag aagcgaagca cttcatcgcg ggcgaatgga cgttgcccgc gcaactggaa accatcccgg tcgtcgatcc gtccgacggc cagccgttcg cgacgatcgc gcgcggcacc gcgcccgaca tcgagcgcgc ggtcgccgcg gcccgcgacg cattcgcagg cccatggggc gccgcgagcg ccgccgagcg cggccgcgtg ctgatgcggc tgtcggcgcg cgtgaccgat tcgatcgagg aactcgccgc gatcgaggcg cgcgataccg gcaagccgct gaagcaggcg cgcgccgatg cagccgcgct cgcacgctac ttcgagttct acgcgggcgc ggccgacaag ctgcacggcg aaaccctccc ctaccaggcc ggctacacgg tgctgacggt gcgcgaaccg cacggcgtca cgggccacat cgtgccgtgg aactacccga tgcagatctt cgggcgcagc gtcggcgcgg cgctcgcggc cggcaacgcg tgcgtggtca agccggccga ggacgcgtgc ctgtccgtgc tgcgcgtcgc cgaactggcc gccgaggccg ggctgccggc cggcgcgctc aacatcgtca ccggctacgg ccatgaagcg ggcgccgcgc tcgcccgcca tccgggcatc gaccacatct cgtttacggg ttcgccggcc accggcaagc tggtcacgca gatggccgcc gagaaccacg tgccggtcac gctcgaactc ggtggcaagt cgccgcaaat cgtgttcgcc gatgccgacc tcgacgccgc gctgcccgtg ctcgtgtccg cgatcgtgca gaacggcggg cagacctgct cggccggcag ccgcgtgctg atcgagcgcg cggtgtacga gccgctcgtc gagcgtctcg ccaccgcgtt caacgggctg cgcgtcggcc cgagccgcgc cgatctcgat tgcggcccgc tgatcaacgc gaagcagcag caacgcgtgt gggatttcct gtccgacgcg cagcacgacg gcattccgat ggccgcgcac gggcaggtcg tcgccgatgc gcccgaaagc ggcttctatc aggcgcccgc gctgttgcgc gacgtgccgc cgtcgcaccg gctcgcgcag gaggaagtgt tcggcccggt gctcgcggcg atgcgtttcg tcgacgagga cgaagccgtc gcgctcgcga acggcacgcc gtacggcctc gtcgcgggca tctggacgcg cgacggcgcg cgccagatgc gtcttgcgcg gcgcctgcgc gcgggtcagg tgttcatcaa caactatggc gcgggcggcg gcgtcgagct gccgttcggc ggcgtcggcc attcgggcca tggccgcgag aagggcttcg aggcgctgta cggcttcacc gcgctgaaga cgatcgcgat ccggcacggc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuceA.00020.c.B1.PS01773
| PURIFICATION DATe: | 3/7/2013 |
| CONCENTRATION: | 74mg/ml |
| OBSERVED MW: | 51kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 25 |
| PERCENT COVERAGE: | 93 |
Protocol Notes
notes unavailable
Validated AA Sequence
MARMADAAER QLDAAARAIV VDEHLTRAQA PRKTHLARAV ARPDARDEAV RRAVRERDGF VLVDETHRRE HRAEHFLLRE PVRRRHVAQQ RGRLIEAAFG RIGDDLPVRG HRNAVVLRVG QEIPHALLLL XVXXRAAIEI GAARADAQPV ERGGETLDER LXHRALDQHA AAGRAGLPAV LHDRGHEHGQ RGVEVGIGEH DLRRLATEFE RDRHVVLGGH LRDQLAGGRR TRKRDVVDAR MAGERGARFM AVAGDDVERA GRQPGLGGQF GDAQHGQARV LGRLDHARVA GRERRADAAP EDLHRVVPRH DVARDAVRFA HRQHRVAGLV GEGFAVQLVG RARVELEVAC ERGCIGARLL QRLAGIARLD RGEFLDRIGH ARRQPHQHAA ALGGARGAPW ACECVAGRGD RALDVGRGAA RDRRERLAVG RIDDRDGFQL RGQRPFARDE VLRF
Validated NT Sequence
cagcgcggtg aagccgtaca gcgcctcgaa gcccttctcg cggccatggc ccgaatggcc gacgccgccg aacggcagct cgacgccgcc gcccgcgcca tagttgttga tgaacacctg acccgcgcgc aggcgccgcg caagacgcat ctggcgcgcg ccgtcgcgcg tccagatgcc cgcgacgagg ccgtacggcg tgccgttcgc gagcgcgacg gcttcgtcct cgtcgacgaa acgcatcgcc gcgagcaccg ggccgaacac ttcctcctgc gcgagccggt gcgacggcgg cacgtcgcgc aacagcgcgg gcgcctgata gaagccgctt tcgggcgcat cggcgacgac ctgcccgtgc gcggccatcg gaatgccgtc gtgctgcgcg tcggacagga aatcccacac gcgttgctgc tgcttcnngt tnatcancgg gccgcaatcg agatcggcgc ggctcgggcc gacgcgcagc ccgttgaacg cggtggcgag acgctcgacg agcggctcgn acaccgcgcg ctcgatcagc acgcggctgc cggccgagca ggtctgcccg ccgttctgca cgatcgcgga cacgagcacg ggcagcgcgg cgtcgaggtc ggcatcggcg aacacgattt gcggcgactt gccaccgagt tcgagcgtga ccggcacgtg gttctcggcg gccatctgcg tgaccagctt gccggtggcc ggcgaacccg taaacgagat gtggtcgatg cccggatggc gggcgagcgc ggcgcccgct tcatggccgt agccggtgac gatgttgagc gcgccggccg gcagcccggc ctcggcggcc agttcggcga cgcgcagcac ggacaggcac gcgtcctcgg ccggcttgac cacgcacgcg ttgccggccg cgagcgccgc gccgacgctg cgcccgaaga tctgcatcgg gtagttccac ggcacgatgt ggcccgtgac gccgtgcggt tcgcgcaccg tcagcaccgt gtagccggcc tggtagggga gggtttcgcc gtgcagcttg tcggccgcgc ccgcgtagaa ctcgaagtag cgtgcgagcg cggctgcatc ggcgcgcgcc tgcttcagcg gcttgccggt atcgcgcgcc tcgatcgcgg cgagttcctc gatcgaatcg gtcacgcgcg ccgacagccg catcagcacg cggccgcgct cggcggcgct cgcggcgccc catgggcctg cgaatgcgtc gcgggccgcg gcgaccgcgc gctcgatgtc gggcgcggtg ccgcgcgcga tcgtcgcgaa cggctggccg tcggacggat cgacgaccgg gatggtttcc agttgcgcgg gcaacgtcca ttcgcccgcg atgaagtgct tcgcttctt
Expressed Protein Sequence
MAHHHHHHME EAKHFIAGEW TLPAQLETIP VVDPSDGQPF ATIARGTAPD IERAVAAARD AFAGPWGAAS AAERGRVLMR LSARVTDSIE ELAAIEARDT GKPLKQARAD AAALARYFEF YAGAADKLHG ETLPYQAGYT VLTVREPHGV TGHIVPWNYP MQIFGRSVGA ALAAGNACVV KPAEDACLSV LRVAELAAEA GLPAGALNIV TGYGHEAGAA LARHPGIDHI SFTGSPATGK LVTQMAAENH VPVTLELGGK SPQIVFADAD LDAALPVLVS AIVQNGGQTC SAGSRVLIER AVYEPLVERL ATAFNGLRVG PSRADLDCGP LINAKQQQRV WDFLSDAQHD GIPMAAHGQV VADAPESGFY QAPALLRDVP PSHRLAQEEV FGPVLAAMRF VDEDEAVALA NGTPYGLVAG IWTRDGARQM RLARRLRAGQ VFINNYGAGG GVELPFGGVG HSGHGREKGF EALYGFTALK TIAIRHG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAG AAGCGAAGCA CTTCATCGCG GGCGAATGGA CGTTGCCCGC GCAACTGGAA ACCATCCCGG TCGTCGATCC GTCCGACGGC CAGCCGTTCG CGACGATCGC GCGCGGCACC GCGCCCGACA TCGAGCGCGC GGTCGCCGCG GCCCGCGACG CATTCGCAGG CCCATGGGGC GCCGCGAGCG CCGCCGAGCG CGGCCGCGTG CTGATGCGGC TGTCGGCGCG CGTGACCGAT TCGATCGAGG AACTCGCCGC GATCGAGGCG CGCGATACCG GCAAGCCGCT GAAGCAGGCG CGCGCCGATG CAGCCGCGCT CGCACGCTAC TTCGAGTTCT ACGCGGGCGC GGCCGACAAG CTGCACGGCG AAACCCTCCC CTACCAGGCC GGCTACACGG TGCTGACGGT GCGCGAACCG CACGGCGTCA CGGGCCACAT CGTGCCGTGG AACTACCCGA TGCAGATCTT CGGGCGCAGC GTCGGCGCGG CGCTCGCGGC CGGCAACGCG TGCGTGGTCA AGCCGGCCGA GGACGCGTGC CTGTCCGTGC TGCGCGTCGC CGAACTGGCC GCCGAGGCCG GGCTGCCGGC CGGCGCGCTC AACATCGTCA CCGGCTACGG CCATGAAGCG GGCGCCGCGC TCGCCCGCCA TCCGGGCATC GACCACATCT CGTTTACGGG TTCGCCGGCC ACCGGCAAGC TGGTCACGCA GATGGCCGCC GAGAACCACG TGCCGGTCAC GCTCGAACTC GGTGGCAAGT CGCCGCAAAT CGTGTTCGCC GATGCCGACC TCGACGCCGC GCTGCCCGTG CTCGTGTCCG CGATCGTGCA GAACGGCGGG CAGACCTGCT CGGCCGGCAG CCGCGTGCTG ATCGAGCGCG CGGTGTACGA GCCGCTCGTC GAGCGTCTCG CCACCGCGTT CAACGGGCTG CGCGTCGGCC CGAGCCGCGC CGATCTCGAT TGCGGCCCGC TGATCAACGC GAAGCAGCAG CAACGCGTGT GGGATTTCCT GTCCGACGCG CAGCACGACG GCATTCCGAT GGCCGCGCAC GGGCAGGTCG TCGCCGATGC GCCCGAAAGC GGCTTCTATC AGGCGCCCGC GCTGTTGCGC GACGTGCCGC CGTCGCACCG GCTCGCGCAG GAGGAAGTGT TCGGCCCGGT GCTCGCGGCG ATGCGTTTCG TCGACGAGGA CGAAGCCGTC GCGCTCGCGA ACGGCACGCC GTACGGCCTC GTCGCGGGCA TCTGGACGCG CGACGGCGCG CGCCAGATGC GTCTTGCGCG GCGCCTGCGC GCGGGTCAGG TGTTCATCAA CAACTATGGC GCGGGCGGCG GCGTCGAGCT GCCGTTCGGC GGCGTCGGCC ATTCGGGCCA TGGCCGCGAG AAGGGCTTCG AGGCGCTGTA CGGCTTCACC GCGCTGAAGA CGATCGCGAT CCGGCACGGC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT