BuceA.00020.a

Methylmalonic acid semialdehyde dehydrogenase

CENTER ID:	BuceA.00020.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystal structure
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00020.a.B1.GE36432	Full length( BuceA.00020.a )		1	506

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00020.a.B1.PS01755	Full length( BuceA.00020.a )		1	506

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_128625
PATRIC ID:	fig\|216591.5.peg.1495
RefSeq:	YP_002230570.1
UniProt:	B4E705

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTVPAYTSD ADVGHYLDGA PVAGRSGRFQ DVFNPALGRA VRRVALAGND EVERAVASAR AAFPAWADTP PIRRARVLHR FLQLMNEHRD TLAAIITAEH GKVFSDAQGE VARGIDIIEF ACGVPQLLKG DFTDQVSTGI DNWTMRQPLG VVAGITPFNF PCMVPCWMFP VALATGNTFV LKPSERDPSA ALFIADLLTQ AGLPAGVFNV VQGDKGAVDA LLDHPDVQAV SFVGSTPIAA YVQQRAVQSG KRVQALGGAK NHLVVMPDAN IDQAVDALIG AAYGSAGERC MAISIAVLVG DVADKIVPAV AERARKLVIG DGMSPEVEMG PIVTGEALKR IEGYIEQGVN EGAQLVVDGR GLRVPGREAG FFTGGTLFDH VKPDMRIYKE EIFGPVLGCV RVKDFGEAVD LINAHEFGNG VSCFTSDGGI AREFARRIQV GMVGINVPIP VPMAWHGFGG WKKSLFGDMH AYGEEGVRFY TRQKSVMQRW SSSIGKGAEF AMPTAK

NT Sequence

ATGACCACGG TTCCCGCCTA TACGTCCGAC GCCGACGTCG GCCACTACCT CGACGGCGCG CCCGTCGCCG GCCGCAGCGG CCGCTTCCAG GACGTCTTCA ATCCCGCTCT CGGTCGCGCG GTGCGCCGCG TCGCGCTCGC CGGCAACGAC GAGGTCGAAC GGGCCGTCGC GTCGGCCCGT GCCGCGTTCC CGGCCTGGGC CGACACGCCG CCGATCCGCC GCGCGCGCGT GCTGCACCGC TTCCTGCAGC TGATGAACGA GCACCGCGAC ACGCTCGCGG CGATCATCAC CGCCGAGCAC GGCAAGGTGT TCTCGGACGC GCAGGGTGAA GTCGCACGCG GCATCGACAT CATCGAATTC GCGTGCGGCG TGCCGCAACT GCTGAAAGGC GACTTCACCG ACCAGGTCAG CACCGGCATC GACAACTGGA CGATGCGCCA GCCGCTCGGC GTCGTCGCGG GCATCACGCC GTTCAACTTC CCGTGCATGG TGCCGTGCTG GATGTTCCCG GTCGCGCTCG CGACGGGCAA CACGTTCGTG CTGAAGCCGA GCGAGCGCGA CCCGTCGGCC GCGCTGTTCA TCGCCGACCT GCTCACGCAG GCAGGCCTGC CCGCCGGCGT GTTCAACGTC GTGCAGGGCG ACAAGGGCGC GGTCGATGCC CTCCTCGACC ACCCGGACGT GCAGGCCGTC AGCTTCGTCG GCTCGACGCC GATCGCGGCC TACGTGCAGC AGCGCGCGGT GCAGTCGGGC AAGCGCGTGC AGGCGCTCGG CGGCGCGAAG AACCACCTCG TCGTGATGCC CGATGCGAAC ATCGACCAGG CCGTCGACGC GCTGATCGGC GCCGCGTACG GCTCGGCCGG CGAGCGCTGC ATGGCGATCA GCATCGCGGT GCTGGTCGGC GACGTGGCCG ACAAGATCGT GCCGGCCGTC GCCGAGCGCG CGCGCAAGCT GGTGATCGGC GACGGGATGT CGCCGGAAGT CGAGATGGGC CCGATCGTCA CCGGCGAAGC GTTGAAGCGC ATCGAAGGCT ATATCGAGCA AGGCGTGAAC GAAGGCGCGC AACTGGTGGT CGACGGCCGC GGGCTGCGCG TGCCGGGCCG CGAGGCAGGC TTCTTCACCG GCGGCACGCT GTTCGATCAC GTGAAACCCG ACATGCGGAT CTACAAGGAA GAGATCTTCG GGCCGGTGCT CGGCTGCGTG CGCGTGAAGG ATTTCGGCGA AGCGGTCGAC CTGATCAACG CGCACGAGTT CGGCAACGGC GTGTCGTGCT TCACGAGCGA CGGCGGGATC GCGCGCGAAT TCGCGCGGCG CATCCAGGTG GGGATGGTCG GCATCAACGT GCCGATTCCG GTGCCGATGG CATGGCACGG CTTCGGCGGC TGGAAGAAGA GCCTGTTCGG CGACATGCAC GCGTACGGCG AGGAAGGCGT GCGCTTCTAC ACGCGCCAGA AGTCGGTGAT GCAGCGCTGG TCGTCGAGCA TCGGCAAGGG CGCCGAATTC GCGATGCCGA CCGCGAAG

Details for BuceA.00020.a.B1.GE36432

HARVESTED ON:	11/21/2012
SEQUENCED ON:	11/27/2012
EXPECTED MW:	55kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	80

Validated AA Sequence

MNEHRDTLAA IITAEHGKVF SDAQGEVARG IDIIEFACGV PQLLKGDFTD QVSTGIDNWT MRQPLGVVAG ITPFNFPCMV PCWMFPVALA TGNTFVLKPS ERDPSAALFI ADLLTQAGLP AGVFNVVQGD XGAVDALLDH PDVXAVSFVG STPIAAYVQQ RAVQSGKRVQ ALGGAKNHLV VMPDANIDQA VDALIGXAYG SAGERCMXIS IAVLVGDVAD KIVPAVAERA RKXVIGDGMS PEVEMGPXVT XEALKRIEGY IEQGVNEGAQ LVVDGRGLRV PGREAGFFTG GTLFDHVKPD MRIYKEEIFG PVLGCVRVKD FGEAVDLINA HEFGNGVSCF TSDGGIAREF ARRIQVGMVG INVPIPVPMA WHGFGGWKKS LFGDMHAYGE EGVRFYTRQK SVMQRWSSSI GKG

Validated NT Sequence

gcgcccttgc cgatgctcga cgaccagcgc tgcatcaccg acttctggcg cgtgtagaag cgcacgcctt cctcgccgta cgcgtgcatg tcgccgaaca ggctcttctt ccagccgccg aagccgtgcc atgccatcgg caccggaatc ggcacgttga tgccgaccat ccccacctgg atgcgccgcg cgaattcgcg cgcgatcccg ccgtcgctcg tgaagcacga cacgccgttg ccgaactcgt gcgcgttgat caggtcgacc gcttcgccga aatccttcac gcgcacgcag ccgagcaccg gcccgaagat ctcttccttg tagatccgca tgtcgggttt cacgtgatcg aacagcgtgc cgccggtgaa gaagcctgcc tcgcggcccg gcacgcgcag cccgcggccg tcgaccacca gttgcgcgcc ttcgttcacg ccttgctcga tatanccttc gatgcgcttc aacgcttcnn cggtgacnat cgggcccatc tcgacttccg gcgacatccc gtcgccgatc accancttgc gcgcgcgctc ggcgacggcc ggcacgatct tgtcngccac gtcgccgacc agcaccgcga tgctgatcgn catgcagcgc tcgccggccg agccgtacgc gncgccgatc agcgcgtcga cggcctggtc gatgttcgca tcgggcatca cgacgaggtg gttcttcgcg ccgccgagcg cctgcacgcg cttgcccgac tgcaccgcgc gctgctgcac gtaggccgcg atnggcgtcg agccgacgaa gctgacggcn tgcacgtccg ggtggtcgag gagggcatcg accgcgccct ngtcgccctg cacgacgttg aacacgccgg cgggcaggcc tgcctgcgtg agcaggtcgg cgatgaacag cgcggccgac gggtcgcgct cgctcggctt cagcacgaac gtgttgcccg tcgcgagcgc gaccgggaac atccagcacg gcaccatgca cgggaagttg aacggcgtga tgcccgcgac gacgccgagc ggctggcgca tcgtccagtt gtcgatgccg gtgctgacct ggtcggtgaa gtcgcctttc agcagttgcg gcacgccgca cgcgaattcg atgatgtcga tgccgcgtgc gacttcaccc tgcgcgtccg agaacacctt gccgtgctcg gcggtgatga tcgccgcgag cgtgtcgcgg tgctcgttca tcagctgcag gaagcggtgc agcacgcgcg cgcggcggat cggcggcgtg tcggcccagg ccgggaacgc ggcacgggcc gacgcgacgg cccgttcgac ctcgtcgttg ccggcgagcg cgacgcggcg caccgcgcga ccgagagcgg gattgaagac gtcctggaag cggccgctgc ggccggcgac gggcgcgccg tcgaggtagt ggccgacgtc ggcgtcggac gtataggcgg gaaccg

Expected Protein Sequence

MAHHHHHHMT TVPAYTSDAD VGHYLDGAPV AGRSGRFQDV FNPALGRAVR RVALAGNDEV ERAVASARAA FPAWADTPPI RRARVLHRFL QLMNEHRDTL AAIITAEHGK VFSDAQGEVA RGIDIIEFAC GVPQLLKGDF TDQVSTGIDN WTMRQPLGVV AGITPFNFPC MVPCWMFPVA LATGNTFVLK PSERDPSAAL FIADLLTQAG LPAGVFNVVQ GDKGAVDALL DHPDVQAVSF VGSTPIAAYV QQRAVQSGKR VQALGGAKNH LVVMPDANID QAVDALIGAA YGSAGERCMA ISIAVLVGDV ADKIVPAVAE RARKLVIGDG MSPEVEMGPI VTGEALKRIE GYIEQGVNEG AQLVVDGRGL RVPGREAGFF TGGTLFDHVK PDMRIYKEEI FGPVLGCVRV KDFGEAVDLI NAHEFGNGVS CFTSDGGIAR EFARRIQVGM VGINVPIPVP MAWHGFGGWK KSLFGDMHAY GEEGVRFYTR QKSVMQRWSS SIGKGAEFAM PTAK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacca cggttcccgc ctatacgtcc gacgccgacg tcggccacta cctcgacggc gcgcccgtcg ccggccgcag cggccgcttc caggacgtct tcaatcccgc tctcggtcgc gcggtgcgcc gcgtcgcgct cgccggcaac gacgaggtcg aacgggccgt cgcgtcggcc cgtgccgcgt tcccggcctg ggccgacacg ccgccgatcc gccgcgcgcg cgtgctgcac cgcttcctgc agctgatgaa cgagcaccgc gacacgctcg cggcgatcat caccgccgag cacggcaagg tgttctcgga cgcgcagggt gaagtcgcac gcggcatcga catcatcgaa ttcgcgtgcg gcgtgccgca actgctgaaa ggcgacttca ccgaccaggt cagcaccggc atcgacaact ggacgatgcg ccagccgctc ggcgtcgtcg cgggcatcac gccgttcaac ttcccgtgca tggtgccgtg ctggatgttc ccggtcgcgc tcgcgacggg caacacgttc gtgctgaagc cgagcgagcg cgacccgtcg gccgcgctgt tcatcgccga cctgctcacg caggcaggcc tgcccgccgg cgtgttcaac gtcgtgcagg gcgacaaggg cgcggtcgat gccctcctcg accacccgga cgtgcaggcc gtcagcttcg tcggctcgac gccgatcgcg gcctacgtgc agcagcgcgc ggtgcagtcg ggcaagcgcg tgcaggcgct cggcggcgcg aagaaccacc tcgtcgtgat gcccgatgcg aacatcgacc aggccgtcga cgcgctgatc ggcgccgcgt acggctcggc cggcgagcgc tgcatggcga tcagcatcgc ggtgctggtc ggcgacgtgg ccgacaagat cgtgccggcc gtcgccgagc gcgcgcgcaa gctggtgatc ggcgacggga tgtcgccgga agtcgagatg ggcccgatcg tcaccggcga agcgttgaag cgcatcgaag gctatatcga gcaaggcgtg aacgaaggcg cgcaactggt ggtcgacggc cgcgggctgc gcgtgccggg ccgcgaggca ggcttcttca ccggcggcac gctgttcgat cacgtgaaac ccgacatgcg gatctacaag gaagagatct tcgggccggt gctcggctgc gtgcgcgtga aggatttcgg cgaagcggtc gacctgatca acgcgcacga gttcggcaac ggcgtgtcgt gcttcacgag cgacggcggg atcgcgcgcg aattcgcgcg gcgcatccag gtggggatgg tcggcatcaa cgtgccgatt ccggtgccga tggcatggca cggcttcggc ggctggaaga agagcctgtt cggcgacatg cacgcgtacg gcgaggaagg cgtgcgcttc tacacgcgcc agaagtcggt gatgcagcgc tggtcgtcga gcatcggcaa gggcgccgaa ttcgcgatgc cgaccgcgaa gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BuceA.00020.a.B1.PS01755

PURIFICATION DATe:	2/20/2013
CONCENTRATION:	48mg/ml
OBSERVED MW:	53kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	80

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCA CGGTTCCCGC CTATACGTCC GACGCCGACG TCGGCCACTA CCTCGACGGC GCGCCCGTCG CCGGCCGCAG CGGCCGCTTC CAGGACGTCT TCAATCCCGC TCTCGGTCGC GCGGTGCGCC GCGTCGCGCT CGCCGGCAAC GACGAGGTCG AACGGGCCGT CGCGTCGGCC CGTGCCGCGT TCCCGGCCTG GGCCGACACG CCGCCGATCC GCCGCGCGCG CGTGCTGCAC CGCTTCCTGC AGCTGATGAA CGAGCACCGC GACACGCTCG CGGCGATCAT CACCGCCGAG CACGGCAAGG TGTTCTCGGA CGCGCAGGGT GAAGTCGCAC GCGGCATCGA CATCATCGAA TTCGCGTGCG GCGTGCCGCA ACTGCTGAAA GGCGACTTCA CCGACCAGGT CAGCACCGGC ATCGACAACT GGACGATGCG CCAGCCGCTC GGCGTCGTCG CGGGCATCAC GCCGTTCAAC TTCCCGTGCA TGGTGCCGTG CTGGATGTTC CCGGTCGCGC TCGCGACGGG CAACACGTTC GTGCTGAAGC CGAGCGAGCG CGACCCGTCG GCCGCGCTGT TCATCGCCGA CCTGCTCACG CAGGCAGGCC TGCCCGCCGG CGTGTTCAAC GTCGTGCAGG GCGACAAGGG CGCGGTCGAT GCCCTCCTCG ACCACCCGGA CGTGCAGGCC GTCAGCTTCG TCGGCTCGAC GCCGATCGCG GCCTACGTGC AGCAGCGCGC GGTGCAGTCG GGCAAGCGCG TGCAGGCGCT CGGCGGCGCG AAGAACCACC TCGTCGTGAT GCCCGATGCG AACATCGACC AGGCCGTCGA CGCGCTGATC GGCGCCGCGT ACGGCTCGGC CGGCGAGCGC TGCATGGCGA TCAGCATCGC GGTGCTGGTC GGCGACGTGG CCGACAAGAT CGTGCCGGCC GTCGCCGAGC GCGCGCGCAA GCTGGTGATC GGCGACGGGA TGTCGCCGGA AGTCGAGATG GGCCCGATCG TCACCGGCGA AGCGTTGAAG CGCATCGAAG GCTATATCGA GCAAGGCGTG AACGAAGGCG CGCAACTGGT GGTCGACGGC CGCGGGCTGC GCGTGCCGGG CCGCGAGGCA GGCTTCTTCA CCGGCGGCAC GCTGTTCGAT CACGTGAAAC CCGACATGCG GATCTACAAG GAAGAGATCT TCGGGCCGGT GCTCGGCTGC GTGCGCGTGA AGGATTTCGG CGAAGCGGTC GACCTGATCA ACGCGCACGA GTTCGGCAAC GGCGTGTCGT GCTTCACGAG CGACGGCGGG ATCGCGCGCG AATTCGCGCG GCGCATCCAG GTGGGGATGG TCGGCATCAA CGTGCCGATT CCGGTGCCGA TGGCATGGCA CGGCTTCGGC GGCTGGAAGA AGAGCCTGTT CGGCGACATG CACGCGTACG GCGAGGAAGG CGTGCGCTTC TACACGCGCC AGAAGTCGGT GATGCAGCGC TGGTCGTCGA GCATCGGCAA GGGCGCCGAA TTCGCGATGC CGACCGCGAA GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT