BuceA.00014.c
Putative uncharacterized protein
| CENTER ID: | BuceA.00014.c |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00014.c.B1.GE36429 | Full length( BuceA.00014.c ) | 1 | 253 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00014.c.B1.PS01770 | Full length( BuceA.00014.c ) | 1 | 253 |
Structures
4MG4
DEPOSITED: 8/28/2013
DETERMINATION: XRay
CLONE: BuceA.00014.c.B1.GE36429
PROTEIN: BuceA.00014.c.B1.PS01770
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|216591.5.peg.4280 |
| RefSeq: | YP_002233211.1 |
| UniProt: | B4EL08 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGNNEKGAIF RSLHRAGQPL ALFNVWDAGS ARVVADAGAV ALATGSWSVA AANGFVDGEQ MPRALMMEVL ERIVRATDLP VTVDLESGYG ERPEDVAETI AMSIRAGAIG CNLEDSFPST GELRDVDEAA ARIAAARQAA DRAGVDYFIN ARTDVFFKAA TETHDERLLD ATLARARAYA AAGADGLFVP GLRSPALIRA LTAASPLPVN VMRVAETPTL AELAEYGVAR ISHGPYPYLQ AMKTLAALVK QGG
NT Sequence
ATGGGCAACA ACGAGAAGGG CGCGATTTTC CGGTCGCTTC ACCGCGCGGG CCAGCCGCTG GCGCTGTTCA ACGTGTGGGA CGCCGGCAGT GCGCGCGTGG TGGCCGACGC CGGCGCCGTC GCGCTGGCCA CCGGCAGCTG GTCGGTCGCG GCAGCCAACG GTTTCGTCGA CGGCGAGCAG ATGCCGCGCG CGCTGATGAT GGAGGTGCTG GAGCGGATCG TGCGTGCGAC GGACTTGCCC GTCACCGTCG ATCTCGAAAG CGGCTACGGC GAGCGGCCGG AAGATGTCGC CGAGACGATC GCGATGAGCA TCCGGGCCGG CGCGATCGGC TGCAATCTCG AAGACAGTTT TCCGTCGACG GGCGAACTGC GCGACGTCGA CGAAGCGGCG GCCCGTATCG CGGCGGCCCG GCAGGCGGCC GATCGCGCGG GCGTCGACTA TTTCATCAAC GCGCGCACCG ACGTGTTCTT CAAGGCGGCA ACCGAGACGC ACGACGAACG ATTGCTCGAC GCGACGCTGG CGCGCGCCCG CGCGTACGCG GCGGCCGGCG CCGACGGCCT GTTCGTGCCC GGCCTGCGCT CGCCGGCGCT CATTCGCGCG CTGACGGCCG CGTCGCCGCT GCCGGTGAAC GTGATGCGCG TCGCGGAAAC GCCGACGCTC GCGGAACTCG CGGAGTACGG CGTGGCGCGC ATCAGCCACG GGCCGTATCC GTATCTGCAG GCAATGAAGA CGCTGGCGGC GCTGGTCAAG CAAGGCGGC
Details for BuceA.00014.c.B1.GE36429
| HARVESTED ON: | 11/21/2012 |
| SEQUENCED ON: | 11/27/2012 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG NNEKGAIFRS LHRAGQPLAL FNVWDAGSAR VVADAGAVAL ATGSWSVAAA NGFVDGEQMP RALMMEVLER IVRATDLPVT VDLESGYGER PEDVAETIAM SIRAGAIGCN LEDSFPSTGE LRDVDEAAAR IAAARQAADR AGVDYFINAR TDVFFKAATE THDERLLDAT LARARAYAAA GADGLFVPGL RSPALIRALT AASPLPVNVM RVAETPTLAE LAEYGVARIS HGPYPYLQAM KTLAALVKQG G
Validated NT Sequence
atcttactca cttagccgcc ttgcttgacc agcgccgcca gcgtcttcat tgcctgcaga tacggatacg gcccgtggct gatgcgcgcc acgccgtact ccgcgagttc cgcgagcgtc ggcgtttccg cgacgcgcat cacgttcacc ggcagcggcg acgcggccgt cagcgcgcga atgagcgccg gcgagcgcag gccgggcacg aacaggccgt cggcgccggc cgccgcgtac gcgcgggcgc gcgccagcgt cgcgtcgagc aatcgttcgt cgtgcgtctc ggttgccgcc ttgaagaaca cgtcggtgcg cgcgttgatg aaatagtcga cgcccgcgcg atcggccgcc tgccgggccg ccgcgatacg ggccgccgct tcgtcgacgt cgcgcagttc gcccgtcgac ggaaaactgt cttcgagatt gcagccgatc gcgccggccc ggatgctcat cgcgatcgtc tcggcgacat cttccggccg ctcgccgtag ccgctttcga gatcgacggt gacgggcaag tccgtcgcac gcacgatccg ctccagcacc tccatcatca gcgcgcgcgg catctgctcg ccgtcgacga aaccgttggc tgccgcgacc gaccagctgc cggtggccag cgcgacggcg ccggcgtcgg ccaccacgcg cgcactgccg gcgtcccaca cgttgaacag cgccagcggc tggcccgcgc ggtgaagcga ccggaaaatc gcgcccttct cgttgttgcc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMG NNEKGAIFRS LHRAGQPLAL FNVWDAGSAR VVADAGAVAL ATGSWSVAAA NGFVDGEQMP RALMMEVLER IVRATDLPVT VDLESGYGER PEDVAETIAM SIRAGAIGCN LEDSFPSTGE LRDVDEAAAR IAAARQAADR AGVDYFINAR TDVFFKAATE THDERLLDAT LARARAYAAA GADGLFVPGL RSPALIRALT AASPLPVNVM RVAETPTLAE LAEYGVARIS HGPYPYLQAM KTLAALVKQG G
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggca acaacgagaa gggcgcgatt ttccggtcgc ttcaccgcgc gggccagccg ctggcgctgt tcaacgtgtg ggacgccggc agtgcgcgcg tggtggccga cgccggcgcc gtcgcgctgg ccaccggcag ctggtcggtc gcggcagcca acggtttcgt cgacggcgag cagatgccgc gcgcgctgat gatggaggtg ctggagcgga tcgtgcgtgc gacggacttg cccgtcaccg tcgatctcga aagcggctac ggcgagcggc cggaagatgt cgccgagacg atcgcgatga gcatccgggc cggcgcgatc ggctgcaatc tcgaagacag ttttccgtcg acgggcgaac tgcgcgacgt cgacgaagcg gcggcccgta tcgcggcggc ccggcaggcg gccgatcgcg cgggcgtcga ctatttcatc aacgcgcgca ccgacgtgtt cttcaaggcg gcaaccgaga cgcacgacga acgattgctc gacgcgacgc tggcgcgcgc ccgcgcgtac gcggcggccg gcgccgacgg cctgttcgtg cccggcctgc gctcgccggc gctcattcgc gcgctgacgg ccgcgtcgcc gctgccggtg aacgtgatgc gcgtcgcgga aacgccgacg ctcgcggaac tcgcggagta cggcgtggcg cgcatcagcc acgggccgta tccgtatctg caggcaatga agacgctggc ggcgctggtc aagcaaggcg gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BuceA.00014.c.B1.PS01770
| PURIFICATION DATe: | 3/12/2013 |
| CONCENTRATION: | 60mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG NNEKGAIFRS LHRAGQPLAL FNVWDAGSAR VVADAGAVAL ATGSWSVAAA NGFVDGEQMP RALMMEVLER IVRATDLPVT VDLESGYGER PEDVAETIAM SIRAGAIGCN LEDSFPSTGE LRDVDEAAAR IAAARQAADR AGVDYFINAR TDVFFKAATE THDERLLDAT LARARAYAAA GADGLFVPGL RSPALIRALT AASPLPVNVM RVAETPTLAE LAEYGVARIS HGPYPYLQAM KTLAALVKQG G
Validated NT Sequence
atcttactca cttagccgcc ttgcttgacc agcgccgcca gcgtcttcat tgcctgcaga tacggatacg gcccgtggct gatgcgcgcc acgccgtact ccgcgagttc cgcgagcgtc ggcgtttccg cgacgcgcat cacgttcacc ggcagcggcg acgcggccgt cagcgcgcga atgagcgccg gcgagcgcag gccgggcacg aacaggccgt cggcgccggc cgccgcgtac gcgcgggcgc gcgccagcgt cgcgtcgagc aatcgttcgt cgtgcgtctc ggttgccgcc ttgaagaaca cgtcggtgcg cgcgttgatg aaatagtcga cgcccgcgcg atcggccgcc tgccgggccg ccgcgatacg ggccgccgct tcgtcgacgt cgcgcagttc gcccgtcgac ggaaaactgt cttcgagatt gcagccgatc gcgccggccc ggatgctcat cgcgatcgtc tcggcgacat cttccggccg ctcgccgtag ccgctttcga gatcgacggt gacgggcaag tccgtcgcac gcacgatccg ctccagcacc tccatcatca gcgcgcgcgg catctgctcg ccgtcgacga aaccgttggc tgccgcgacc gaccagctgc cggtggccag cgcgacggcg ccggcgtcgg ccaccacgcg cgcactgccg gcgtcccaca cgttgaacag cgccagcggc tggcccgcgc ggtgaagcga ccggaaaatc gcgcccttct cgttgttgcc catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMG NNEKGAIFRS LHRAGQPLAL FNVWDAGSAR VVADAGAVAL ATGSWSVAAA NGFVDGEQMP RALMMEVLER IVRATDLPVT VDLESGYGER PEDVAETIAM SIRAGAIGCN LEDSFPSTGE LRDVDEAAAR IAAARQAADR AGVDYFINAR TDVFFKAATE THDERLLDAT LARARAYAAA GADGLFVPGL RSPALIRALT AASPLPVNVM RVAETPTLAE LAEYGVARIS HGPYPYLQAM KTLAALVKQG G
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGCA ACAACGAGAA GGGCGCGATT TTCCGGTCGC TTCACCGCGC GGGCCAGCCG CTGGCGCTGT TCAACGTGTG GGACGCCGGC AGTGCGCGCG TGGTGGCCGA CGCCGGCGCC GTCGCGCTGG CCACCGGCAG CTGGTCGGTC GCGGCAGCCA ACGGTTTCGT CGACGGCGAG CAGATGCCGC GCGCGCTGAT GATGGAGGTG CTGGAGCGGA TCGTGCGTGC GACGGACTTG CCCGTCACCG TCGATCTCGA AAGCGGCTAC GGCGAGCGGC CGGAAGATGT CGCCGAGACG ATCGCGATGA GCATCCGGGC CGGCGCGATC GGCTGCAATC TCGAAGACAG TTTTCCGTCG ACGGGCGAAC TGCGCGACGT CGACGAAGCG GCGGCCCGTA TCGCGGCGGC CCGGCAGGCG GCCGATCGCG CGGGCGTCGA CTATTTCATC AACGCGCGCA CCGACGTGTT CTTCAAGGCG GCAACCGAGA CGCACGACGA ACGATTGCTC GACGCGACGC TGGCGCGCGC CCGCGCGTAC GCGGCGGCCG GCGCCGACGG CCTGTTCGTG CCCGGCCTGC GCTCGCCGGC GCTCATTCGC GCGCTGACGG CCGCGTCGCC GCTGCCGGTG AACGTGATGC GCGTCGCGGA AACGCCGACG CTCGCGGAAC TCGCGGAGTA CGGCGTGGCG CGCATCAGCC ACGGGCCGTA TCCGTATCTG CAGGCAATGA AGACGCTGGC GGCGCTGGTC AAGCAAGGCG GCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT