BuceA.00014.a
Putative lyase/mutase
| CENTER ID: | BuceA.00014.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00014.a.B1.GE36428 | Full length( BuceA.00014.a ) | 1 | 286 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00014.a.B1.PS01746 | Full length( BuceA.00014.a ) | 1 | 286 |
Structures
4LSB
DEPOSITED: 7/22/2013
DETERMINATION: XRay
CLONE: BuceA.00014.a.B1.GE36428
PROTEIN: BuceA.00014.a.B1.PS01746
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|216591.5.peg.5931 |
| RefSeq: | YP_002234755.1 |
| UniProt: | B4EFV2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIRSDLQARH AEAFRALHTR PGAFIIPNPW DAGTARLLAM AGFEALATTS AGYAFSKGQP DNAIDRDAML DHIADLVAAG GLPVSADLEN GFGDAPGTVA ETIRLAAEAG AVGGSIEDAT GRADTPIYAR DASVERIAAA VDAARALPFP FTLTARCENY LHGRRDLDDT IARLVAYRDA GADVLYAPGI TDADEIAAVT RAVGAPVNVV MGLQGGLLSL DELAALGVKR VSVGGALARA ALGAFLRAAT EMRRDGTFTF TQAAVPGRDI NRWFAAPDNS PILFDE
NT Sequence
ATGATCCGTT CCGACCTGCA AGCCCGCCAC GCCGAAGCTT TCCGTGCGCT GCATACGCGC CCCGGCGCGT TCATCATCCC GAACCCGTGG GACGCCGGCA CCGCGCGCCT GCTCGCGATG GCCGGCTTCG AGGCGCTCGC CACGACCAGC GCCGGCTACG CGTTTTCGAA AGGGCAGCCC GACAACGCGA TTGACCGCGA CGCGATGCTC GATCACATCG CCGATCTCGT CGCCGCGGGG GGCCTGCCGG TGAGCGCCGA TCTCGAGAAC GGCTTCGGCG ACGCGCCCGG CACGGTGGCC GAAACGATCC GGCTGGCGGC CGAAGCCGGC GCGGTCGGTG GCTCGATCGA GGATGCGACC GGTCGCGCCG ATACGCCGAT CTATGCGCGC GACGCGTCGG TCGAGCGCAT CGCGGCGGCC GTCGACGCGG CGCGCGCGCT GCCGTTTCCG TTCACGCTGA CCGCCCGCTG CGAAAACTAC CTGCACGGCC GCCGCGACCT CGACGACACG ATCGCGCGGC TCGTCGCCTA TCGCGACGCC GGCGCCGACG TGCTGTACGC GCCCGGCATC ACCGACGCCG ACGAGATCGC GGCGGTGACG CGGGCGGTCG GCGCGCCGGT CAATGTCGTC ATGGGCCTGC AGGGCGGGCT GCTGAGCCTC GACGAACTGG CGGCGCTCGG CGTGAAGCGT GTCAGCGTCG GCGGCGCGCT GGCGCGGGCC GCGCTCGGCG CGTTCCTGCG CGCGGCGACG GAGATGAGGC GCGACGGCAC CTTCACCTTT ACCCAGGCGG CCGTGCCGGG GCGCGATATC AACCGCTGGT TTGCGGCGCC GGATAATTCG CCGATTTTGT TCGACGAA
Details for BuceA.00014.a.B1.GE36428
| HARVESTED ON: | 11/21/2012 |
| SEQUENCED ON: | 11/27/2012 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMI RSDLQARHAE AFRALHTRPG AFIIPNPWDA GTARLLAMAG FEALATTSAG YAFSKGQPDN AIDRDAMLDH IADLVAAGGL PVSADLENGF GDAPGTVAET IRLAAEAGAV GGSIEDATGR ADTPIYARDA SVERIAAAVD AARALPFPFT LTARCENYLH GRRDLDDTIA RLVAYRDAGA DVLYAPGITD ADEIAAVTRA VGAPVNVVMG LQGGLLSLDE LAALGVKRVS VGGALARAAL GAFLRAATEM RRDGTFTFTQ AAVPGRDINR WFAAPDNSPI LFDE
Validated NT Sequence
tcttactcac ttattcgtcg aacaaaatcg gcgaattatc cggcgccgca aaccagcggt tgatatcgcg ccccggcacg gccgcctggg taaaggtgaa ggtgccgtcg cgcctcatct ccgtcgccgc gcgcaggaac gcgccgagcg cggcccgcgc cagcgcgccg ccgacgctga cacgcttcac gccgagcgcc gccagttcgt cgaggctcag cagcccgccc tgcaggccca tgacgacatt gaccggcgcg ccgaccgccc gcgtcaccgc cgcgatctcg tcggcgtcgg tgatgccggg cgcgtacagc acgtcggcgc cggcgtcgcg ataggcgacg agccgcgcga tcgtgtcgtc gaggtcgcgg cggccgtgca ggtagttttc gcagcgggcg gtcagcgtga acggaaacgg cagcgcgcgc gccgcgtcga cggccgccgc gatgcgctcg accgacgcgt cgcgcgcata gatcggcgta tcggcgcgac cggtcgcatc ctcgatcgag ccaccgaccg cgccggcttc ggccgccagc cggatcgttt cggccaccgt gccgggcgcg tcgccgaagc cgttctcgag atcggcgctc accggcaggc cccccgcggc gacgagatcg gcgatgtgat cgagcatcgc gtcgcggtca atcgcgttgt cgggctgccc tttcgaaaac gcgtagccgg cgctggtcgt ggcgagcgcc tcgaagccgg ccatcgcgag caggcgcgcg gtgccggcgt cccacgggtt cgggatgatg aacgcgccgg ggcgcgtatg cagcgcacgg aaagcttcgg cgtggcgggc ttgcaggtcg gaacggatca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMI RSDLQARHAE AFRALHTRPG AFIIPNPWDA GTARLLAMAG FEALATTSAG YAFSKGQPDN AIDRDAMLDH IADLVAAGGL PVSADLENGF GDAPGTVAET IRLAAEAGAV GGSIEDATGR ADTPIYARDA SVERIAAAVD AARALPFPFT LTARCENYLH GRRDLDDTIA RLVAYRDAGA DVLYAPGITD ADEIAAVTRA VGAPVNVVMG LQGGLLSLDE LAALGVKRVS VGGALARAAL GAFLRAATEM RRDGTFTFTQ AAVPGRDINR WFAAPDNSPI LFDE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcc gttccgacct gcaagcccgc cacgccgaag ctttccgtgc gctgcatacg cgccccggcg cgttcatcat cccgaacccg tgggacgccg gcaccgcgcg cctgctcgcg atggccggct tcgaggcgct cgccacgacc agcgccggct acgcgttttc gaaagggcag cccgacaacg cgattgaccg cgacgcgatg ctcgatcaca tcgccgatct cgtcgccgcg gggggcctgc cggtgagcgc cgatctcgag aacggcttcg gcgacgcgcc cggcacggtg gccgaaacga tccggctggc ggccgaagcc ggcgcggtcg gtggctcgat cgaggatgcg accggtcgcg ccgatacgcc gatctatgcg cgcgacgcgt cggtcgagcg catcgcggcg gccgtcgacg cggcgcgcgc gctgccgttt ccgttcacgc tgaccgcccg ctgcgaaaac tacctgcacg gccgccgcga cctcgacgac acgatcgcgc ggctcgtcgc ctatcgcgac gccggcgccg acgtgctgta cgcgcccggc atcaccgacg ccgacgagat cgcggcggtg acgcgggcgg tcggcgcgcc ggtcaatgtc gtcatgggcc tgcagggcgg gctgctgagc ctcgacgaac tggcggcgct cggcgtgaag cgtgtcagcg tcggcggcgc gctggcgcgg gccgcgctcg gcgcgttcct gcgcgcggcg acggagatga ggcgcgacgg caccttcacc tttacccagg cggccgtgcc ggggcgcgat atcaaccgct ggtttgcggc gccggataat tcgccgattt tgttcgacga atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BuceA.00014.a.B1.PS01746
| PURIFICATION DATe: | 2/14/2013 |
| CONCENTRATION: | 47mg/ml |
| OBSERVED MW: | 31kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI RSDLQARHAE AFRALHTRPG AFIIPNPWDA GTARLLAMAG FEALATTSAG YAFSKGQPDN AIDRDAMLDH IADLVAAGGL PVSADLENGF GDAPGTVAET IRLAAEAGAV GGSIEDATGR ADTPIYARDA SVERIAAAVD AARALPFPFT LTARCENYLH GRRDLDDTIA RLVAYRDAGA DVLYAPGITD ADEIAAVTRA VGAPVNVVMG LQGGLLSLDE LAALGVKRVS VGGALARAAL GAFLRAATEM RRDGTFTFTQ AAVPGRDINR WFAAPDNSPI LFDE
Validated NT Sequence
tcttactcac ttattcgtcg aacaaaatcg gcgaattatc cggcgccgca aaccagcggt tgatatcgcg ccccggcacg gccgcctggg taaaggtgaa ggtgccgtcg cgcctcatct ccgtcgccgc gcgcaggaac gcgccgagcg cggcccgcgc cagcgcgccg ccgacgctga cacgcttcac gccgagcgcc gccagttcgt cgaggctcag cagcccgccc tgcaggccca tgacgacatt gaccggcgcg ccgaccgccc gcgtcaccgc cgcgatctcg tcggcgtcgg tgatgccggg cgcgtacagc acgtcggcgc cggcgtcgcg ataggcgacg agccgcgcga tcgtgtcgtc gaggtcgcgg cggccgtgca ggtagttttc gcagcgggcg gtcagcgtga acggaaacgg cagcgcgcgc gccgcgtcga cggccgccgc gatgcgctcg accgacgcgt cgcgcgcata gatcggcgta tcggcgcgac cggtcgcatc ctcgatcgag ccaccgaccg cgccggcttc ggccgccagc cggatcgttt cggccaccgt gccgggcgcg tcgccgaagc cgttctcgag atcggcgctc accggcaggc cccccgcggc gacgagatcg gcgatgtgat cgagcatcgc gtcgcggtca atcgcgttgt cgggctgccc tttcgaaaac gcgtagccgg cgctggtcgt ggcgagcgcc tcgaagccgg ccatcgcgag caggcgcgcg gtgccggcgt cccacgggtt cgggatgatg aacgcgccgg ggcgcgtatg cagcgcacgg aaagcttcgg cgtggcgggc ttgcaggtcg gaacggatca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMI RSDLQARHAE AFRALHTRPG AFIIPNPWDA GTARLLAMAG FEALATTSAG YAFSKGQPDN AIDRDAMLDH IADLVAAGGL PVSADLENGF GDAPGTVAET IRLAAEAGAV GGSIEDATGR ADTPIYARDA SVERIAAAVD AARALPFPFT LTARCENYLH GRRDLDDTIA RLVAYRDAGA DVLYAPGITD ADEIAAVTRA VGAPVNVVMG LQGGLLSLDE LAALGVKRVS VGGALARAAL GAFLRAATEM RRDGTFTFTQ AAVPGRDINR WFAAPDNSPI LFDE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCC GTTCCGACCT GCAAGCCCGC CACGCCGAAG CTTTCCGTGC GCTGCATACG CGCCCCGGCG CGTTCATCAT CCCGAACCCG TGGGACGCCG GCACCGCGCG CCTGCTCGCG ATGGCCGGCT TCGAGGCGCT CGCCACGACC AGCGCCGGCT ACGCGTTTTC GAAAGGGCAG CCCGACAACG CGATTGACCG CGACGCGATG CTCGATCACA TCGCCGATCT CGTCGCCGCG GGGGGCCTGC CGGTGAGCGC CGATCTCGAG AACGGCTTCG GCGACGCGCC CGGCACGGTG GCCGAAACGA TCCGGCTGGC GGCCGAAGCC GGCGCGGTCG GTGGCTCGAT CGAGGATGCG ACCGGTCGCG CCGATACGCC GATCTATGCG CGCGACGCGT CGGTCGAGCG CATCGCGGCG GCCGTCGACG CGGCGCGCGC GCTGCCGTTT CCGTTCACGC TGACCGCCCG CTGCGAAAAC TACCTGCACG GCCGCCGCGA CCTCGACGAC ACGATCGCGC GGCTCGTCGC CTATCGCGAC GCCGGCGCCG ACGTGCTGTA CGCGCCCGGC ATCACCGACG CCGACGAGAT CGCGGCGGTG ACGCGGGCGG TCGGCGCGCC GGTCAATGTC GTCATGGGCC TGCAGGGCGG GCTGCTGAGC CTCGACGAAC TGGCGGCGCT CGGCGTGAAG CGTGTCAGCG TCGGCGGCGC GCTGGCGCGG GCCGCGCTCG GCGCGTTCCT GCGCGCGGCG ACGGAGATGA GGCGCGACGG CACCTTCACC TTTACCCAGG CGGCCGTGCC GGGGCGCGAT ATCAACCGCT GGTTTGCGGC GCCGGATAAT TCGCCGATTT TGTTCGACGA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT