BuceA.00010.z
Putative D-beta-hydroxybutyrate dehydrogenase
| CENTER ID: | BuceA.00010.z |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00010.z.B1.GE36354 | Full length( BuceA.00010.z ) | 1 | 261 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4TRR
DEPOSITED: 6/17/2014
DETERMINATION: XRay
CLONE: BuceA.00010.z.B1.GE36354
PROTEIN: BuceA.00010.z.B1.PS01812
8DT1
DEPOSITED: 7/25/2022
DETERMINATION: XRay
CLONE: BuceA.00010.z.B1.GE36354
PROTEIN: BuceA.00010.z.B1.PS01812
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126618 |
| PATRIC ID: | fig|216591.5.peg.3694 |
| RefSeq: | YP_002232655.1 |
| UniProt: | B4EG08 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNLNGKTAV VTGAASGIGK EIALELAKAG AAVAIADLNQ DGANAVADEI NKAGGKAIGV AMDVTNEEAV NTGIDKVAEA FGSVDILVSN AGIQIVNPIE NYSFADWKKM QAIHVDGAFL TTKAALKHMY KDDRGGVVIY MGSVHSHEAS PLKSAYVTAK HGLLGLARVL AKEGAKHNVR SHVVCPGFVR TPLVDKQIPE QAKELGISEE EVIKKVMLGN TVDGVFTTVQ DVAQTVLFLS AFPSAALTGQ SFIVSHGWFM Q
NT Sequence
ATGAGCAACC TGAATGGCAA GACCGCAGTC GTCACGGGCG CCGCGAGCGG CATCGGCAAG GAAATCGCAC TGGAGCTCGC CAAGGCAGGC GCGGCCGTCG CGATCGCCGA CCTGAACCAG GACGGCGCGA ACGCGGTCGC CGACGAGATC AACAAGGCAG GCGGCAAGGC GATCGGCGTC GCGATGGACG TGACCAACGA GGAAGCCGTA AACACCGGCA TCGACAAGGT GGCCGAAGCG TTCGGCTCGG TCGACATCCT CGTGTCGAAC GCGGGCATCC AGATCGTCAA CCCGATCGAG AACTACTCGT TCGCCGACTG GAAGAAGATG CAGGCGATCC ACGTGGACGG CGCGTTCCTG ACAACGAAGG CCGCGCTCAA GCACATGTAC AAGGACGATC GCGGCGGCGT CGTGATCTAC ATGGGTTCGG TGCACTCGCA CGAAGCGTCG CCGCTGAAGT CGGCGTACGT GACGGCCAAG CACGGTCTGC TCGGCCTGGC GCGCGTGCTG GCAAAGGAAG GCGCGAAGCA CAACGTGCGC TCGCACGTCG TGTGTCCGGG CTTCGTGCGC ACGCCGCTGG TCGACAAGCA GATTCCGGAG CAGGCGAAGG AGCTCGGCAT CAGCGAAGAG GAAGTGATCA AGAAGGTGAT GCTCGGCAAC ACGGTCGACG GCGTGTTCAC GACGGTGCAG GACGTCGCGC AGACGGTGCT GTTCCTGTCG GCGTTCCCGA GCGCCGCGCT CACGGGTCAG TCGTTCATCG TCAGCCACGG CTGGTTCATG CAA
Details for BuceA.00010.z.B1.GE36354
| HARVESTED ON: | 10/23/2012 |
| SEQUENCED ON: | 7/19/2017 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS NLNGKTAVVT GAASGIGKEI ALELAKAGAA VAIADLNQDG ANAVADEINK AGGKAIGVAM DVTNEEAVNT GIDKVAEAFG SVDILVSNAG IQIVNPIENY SFADWKKMQA IHVDGAFLTT KAALKHMYKD DRGGVVIYMG SVHSHEASPL KSAYVTAKHG LLGLARVLAK EGAKHNVRSH VVCPGFVRTP LVDKQIPEQA KELGISEEEV IKKVMLGNTV DGVFTTVQDV AQTVLFLSAF PSAALTGQSF IVSHGWFMQ
Validated NT Sequence
atcttactca cttattgcat gaaccagccg tggctgacga tgaacgactg acccgtgagc gcggcgctcg ggaacgccga caggaacagc accgtctgcg cgacgtcctg caccgtcgtg aacacgccgt cgaccgtgtt gccgagcatc accttcttga tcacttcctc ttcgctgatg ccgagctcct tcgcctgctc cggaatctgc ttgtcgacca gcggcgtgcg cacgaagccc ggacacacga cgtgcgagcg cacgttgtgc ttcgcgcctt cctttgccag cacgcgcgcc aggccgagca gaccgtgctt ggccgtcacg tacgccgact tcagcggcga cgcttcgtgc gagtgcaccg aacccatgta gatcacgacg ccgccgcgat cgtccttgta catgtgcttg agcgcggcct tcgttgtcag gaacgcgccg tccacgtgga tcgcctgcat cttcttccag tcggcgaacg agtagttctc gatcgggttg acgatctgga tgcccgcgtt cgacacgagg atgtcgaccg agccgaacgc ttcggccacc ttgtcgatgc cggtgtttac ggcttcctcg ttggtcacgt ccatcgcgac gccgatcgcc ttgccgcctg ccttgttgat ctcgtcggcg accgcgttcg cgccgtcctg gttcaggtcg gcgatcgcga cggccgcgcc tgccttggcg agctccagtg cgatttcctt gccgatgccg ctcgcggcgc ccgtgacgac tgcggtcttg ccattcaggt tgctcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMS NLNGKTAVVT GAASGIGKEI ALELAKAGAA VAIADLNQDG ANAVADEINK AGGKAIGVAM DVTNEEAVNT GIDKVAEAFG SVDILVSNAG IQIVNPIENY SFADWKKMQA IHVDGAFLTT KAALKHMYKD DRGGVVIYMG SVHSHEASPL KSAYVTAKHG LLGLARVLAK EGAKHNVRSH VVCPGFVRTP LVDKQIPEQA KELGISEEEV IKKVMLGNTV DGVFTTVQDV AQTVLFLSAF PSAALTGQSF IVSHGWFMQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca acctgaatgg caagaccgca gtcgtcacgg gcgccgcgag cggcatcggc aaggaaatcg cactggagct cgccaaggca ggcgcggccg tcgcgatcgc cgacctgaac caggacggcg cgaacgcggt cgccgacgag atcaacaagg caggcggcaa ggcgatcggc gtcgcgatgg acgtgaccaa cgaggaagcc gtaaacaccg gcatcgacaa ggtggccgaa gcgttcggct cggtcgacat cctcgtgtcg aacgcgggca tccagatcgt caacccgatc gagaactact cgttcgccga ctggaagaag atgcaggcga tccacgtgga cggcgcgttc ctgacaacga aggccgcgct caagcacatg tacaaggacg atcgcggcgg cgtcgtgatc tacatgggtt cggtgcactc gcacgaagcg tcgccgctga agtcggcgta cgtgacggcc aagcacggtc tgctcggcct ggcgcgcgtg ctggcaaagg aaggcgcgaa gcacaacgtg cgctcgcacg tcgtgtgtcc gggcttcgtg cgcacgccgc tggtcgacaa gcagattccg gagcaggcga aggagctcgg catcagcgaa gaggaagtga tcaagaaggt gatgctcggc aacacggtcg acggcgtgtt cacgacggtg caggacgtcg cgcagacggt gctgttcctg tcggcgttcc cgagcgccgc gctcacgggt cagtcgttca tcgtcagcca cggctggttc atgcaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t