BuceA.00010.y
Putative short chain dehydrogenase
| CENTER ID: | BuceA.00010.y |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00010.y.B1.GE36353 | Full length( BuceA.00010.y ) | 1 | 237 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00010.y.B1.PS01748 | Full length( BuceA.00010.y ) | 1 | 237 |
Structures
5U4S
DEPOSITED: 12/6/2016
DETERMINATION: XRay
CLONE: BuceA.00010.y.B1.GE36353
PROTEIN: BuceA.00010.y.B1.PS01748
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_159159 |
| PATRIC ID: | fig|216591.5.peg.5899 |
| RefSeq: | YP_002234728.1 |
| UniProt: | B4EFS5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQIEGCVACV TGADRGLGAG LLEALLERGA RKVYAGVRKK ECLSDVGPRV VPVEIDITNV EQVARAASRA KDITLLINNA GLNRMQPVLE AHDPEAARAE MEVNYFGTLN MMRAFSPALK SNGGAIINVL SILARVALPS MASLSASKAA ALRMTEGARA ELAPHRVRVI SVLPGPIDTE MSRNVPPPKI AVREAVDAVL AALEGGADEV YMGAMAQEIA QGLAADRQAL HARLLTP
NT Sequence
ATGCAAATTG AAGGCTGCGT GGCATGTGTG ACGGGTGCCG ACCGTGGGCT AGGTGCGGGC TTGCTTGAAG CGTTGCTGGA GCGGGGCGCG CGCAAGGTCT ATGCGGGCGT CCGGAAAAAG GAATGTCTTT CGGACGTTGG TCCGCGCGTC GTGCCGGTTG AAATAGACAT CACGAACGTC GAGCAGGTGG CGAGGGCGGC GTCGCGTGCC AAGGACATCA CGCTCCTGAT CAACAACGCG GGGCTGAACC GGATGCAACC GGTACTGGAA GCGCATGACC CGGAAGCGGC GCGTGCCGAA ATGGAAGTGA ACTACTTCGG GACGTTGAAC ATGATGCGTG CATTTTCACC GGCACTCAAA TCGAATGGCG GCGCCATCAT CAACGTTCTT TCGATACTCG CGCGCGTCGC GCTGCCGTCC ATGGCGTCGT TGAGTGCATC GAAGGCTGCG GCGCTTCGAA TGACGGAAGG CGCACGCGCC GAACTCGCGC CGCATCGCGT TCGGGTCATC TCCGTGCTGC CCGGACCGAT CGACACGGAA ATGAGCCGGA ACGTTCCCCC TCCGAAGATT GCGGTCCGAG AAGCAGTCGA CGCCGTGCTG GCCGCATTGG AAGGCGGAGC GGACGAGGTC TACATGGGCG CGATGGCACA AGAAATCGCG CAGGGACTCG CCGCGGATCG CCAGGCGCTG CACGCGCGCC TTTTGACGCC T
Details for BuceA.00010.y.B1.GE36353
| HARVESTED ON: | 10/23/2012 |
| SEQUENCED ON: | 10/26/2012 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHHM QIEGCVACVT GADRGLGAGL LEALLERGAR KVYAGVRKKE CLSDVGPRVV PVEIDITNVE QVARAASRAK DITLLINNAG LNRMQPVLEA HDPEAARAEM EVNYFGTLNM MRAFSPALKS NGGAIINVLS ILARVALPSM ASLSASKAAA LRMTEGARAE LAPHRVRVIS VLPGPIDTEM SRNVPPPKIA VREAVDAVLA ALEGGADEVY MGAMAQEIAQ GLAADRQALH ARLLTP
Validated NT Sequence
atcttactca cttaaggcgt caaaaggcgc gcgtgcagcg cctggcgatc cgcggcgagt ccctgcgcga tttcttgtgc catcgcgccc atgtagacct cgtccgctcc gccttccaat gcggccagca cggcgtcgac tgcttctcgg accgcaatct tcggaggggg aacgttccgg ctcatttccg tgtcgatcgg tccgggcagc acggagatga cccgaacgcg atgcggcgcg agttcggcgc gtgcgccttc cgtcattcga agcgccgcag ccttcgatgc actcaacgac gccatggacg gcagcgcgac gcgcgcgagt atcgaaagaa cgttgatgat ggcgccgcca ttcgatttga gtgccggtga aaatgcacgc atcatgttca acgtcccgaa gtagttcact tccatttcgg cacgcgccgc ttccgggtca tgcgcttcca gtaccggttg catccggttc agccccgcgt tgttgatcag gagcgtgatg tccttggcac gcgacgccgc cctcgccacc tgctcgacgt tcgtgatgtc tatttcaacc ggcacgacgc gcggaccaac gtccgaaaga cattcctttt tccggacgcc cgcatagacc ttgcgcgcgc cccgctccag caacgcttca agcaagcccg cacctagccc acggtcggca cccgtcacac atgccacgca gccttcaatt tgcatatggt ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMQ IEGCVACVTG ADRGLGAGLL EALLERGARK VYAGVRKKEC LSDVGPRVVP VEIDITNVEQ VARAASRAKD ITLLINNAGL NRMQPVLEAH DPEAARAEME VNYFGTLNMM RAFSPALKSN GGAIINVLSI LARVALPSMA SLSASKAAAL RMTEGARAEL APHRVRVISV LPGPIDTEMS RNVPPPKIAV REAVDAVLAA LEGGADEVYM GAMAQEIAQG LAADRQALHA RLLTP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa ttgaaggctg cgtggcatgt gtgacgggtg ccgaccgtgg gctaggtgcg ggcttgcttg aagcgttgct ggagcggggc gcgcgcaagg tctatgcggg cgtccggaaa aaggaatgtc tttcggacgt tggtccgcgc gtcgtgccgg ttgaaataga catcacgaac gtcgagcagg tggcgagggc ggcgtcgcgt gccaaggaca tcacgctcct gatcaacaac gcggggctga accggatgca accggtactg gaagcgcatg acccggaagc ggcgcgtgcc gaaatggaag tgaactactt cgggacgttg aacatgatgc gtgcattttc accggcactc aaatcgaatg gcggcgccat catcaacgtt ctttcgatac tcgcgcgcgt cgcgctgccg tccatggcgt cgttgagtgc atcgaaggct gcggcgcttc gaatgacgga aggcgcacgc gccgaactcg cgccgcatcg cgttcgggtc atctccgtgc tgcccggacc gatcgacacg gaaatgagcc ggaacgttcc ccctccgaag attgcggtcc gagaagcagt cgacgccgtg ctggccgcat tggaaggcgg agcggacgag gtctacatgg gcgcgatggc acaagaaatc gcgcagggac tcgccgcgga tcgccaggcg ctgcacgcgc gccttttgac gccttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BuceA.00010.y.B1.PS01748
| PURIFICATION DATe: | 2/14/2013 |
| CONCENTRATION: | 46.9mg/ml |
| OBSERVED MW: | 26kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHHM QIEGCVACVT GADRGLGAGL LEALLERGAR KVYAGVRKKE CLSDVGPRVV PVEIDITNVE QVARAASRAK DITLLINNAG LNRMQPVLEA HDPEAARAEM EVNYFGTLNM MRAFSPALKS NGGAIINVLS ILARVALPSM ASLSASKAAA LRMTEGARAE LAPHRVRVIS VLPGPIDTEM SRNVPPPKIA VREAVDAVLA ALEGGADEVY MGAMAQEIAQ GLAADRQALH ARLLTP
Validated NT Sequence
atcttactca cttaaggcgt caaaaggcgc gcgtgcagcg cctggcgatc cgcggcgagt ccctgcgcga tttcttgtgc catcgcgccc atgtagacct cgtccgctcc gccttccaat gcggccagca cggcgtcgac tgcttctcgg accgcaatct tcggaggggg aacgttccgg ctcatttccg tgtcgatcgg tccgggcagc acggagatga cccgaacgcg atgcggcgcg agttcggcgc gtgcgccttc cgtcattcga agcgccgcag ccttcgatgc actcaacgac gccatggacg gcagcgcgac gcgcgcgagt atcgaaagaa cgttgatgat ggcgccgcca ttcgatttga gtgccggtga aaatgcacgc atcatgttca acgtcccgaa gtagttcact tccatttcgg cacgcgccgc ttccgggtca tgcgcttcca gtaccggttg catccggttc agccccgcgt tgttgatcag gagcgtgatg tccttggcac gcgacgccgc cctcgccacc tgctcgacgt tcgtgatgtc tatttcaacc ggcacgacgc gcggaccaac gtccgaaaga cattcctttt tccggacgcc cgcatagacc ttgcgcgcgc cccgctccag caacgcttca agcaagcccg cacctagccc acggtcggca cccgtcacac atgccacgca gccttcaatt tgcatatggt ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMQ IEGCVACVTG ADRGLGAGLL EALLERGARK VYAGVRKKEC LSDVGPRVVP VEIDITNVEQ VARAASRAKD ITLLINNAGL NRMQPVLEAH DPEAARAEME VNYFGTLNMM RAFSPALKSN GGAIINVLSI LARVALPSMA SLSASKAAAL RMTEGARAEL APHRVRVISV LPGPIDTEMS RNVPPPKIAV REAVDAVLAA LEGGADEVYM GAMAQEIAQG LAADRQALHA RLLTP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAA TTGAAGGCTG CGTGGCATGT GTGACGGGTG CCGACCGTGG GCTAGGTGCG GGCTTGCTTG AAGCGTTGCT GGAGCGGGGC GCGCGCAAGG TCTATGCGGG CGTCCGGAAA AAGGAATGTC TTTCGGACGT TGGTCCGCGC GTCGTGCCGG TTGAAATAGA CATCACGAAC GTCGAGCAGG TGGCGAGGGC GGCGTCGCGT GCCAAGGACA TCACGCTCCT GATCAACAAC GCGGGGCTGA ACCGGATGCA ACCGGTACTG GAAGCGCATG ACCCGGAAGC GGCGCGTGCC GAAATGGAAG TGAACTACTT CGGGACGTTG AACATGATGC GTGCATTTTC ACCGGCACTC AAATCGAATG GCGGCGCCAT CATCAACGTT CTTTCGATAC TCGCGCGCGT CGCGCTGCCG TCCATGGCGT CGTTGAGTGC ATCGAAGGCT GCGGCGCTTC GAATGACGGA AGGCGCACGC GCCGAACTCG CGCCGCATCG CGTTCGGGTC ATCTCCGTGC TGCCCGGACC GATCGACACG GAAATGAGCC GGAACGTTCC CCCTCCGAAG ATTGCGGTCC GAGAAGCAGT CGACGCCGTG CTGGCCGCAT TGGAAGGCGG AGCGGACGAG GTCTACATGG GCGCGATGGC ACAAGAAATC GCGCAGGGAC TCGCCGCGGA TCGCCAGGCG CTGCACGCGC GCCTTTTGAC GCCTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT