BuceA.00010.x
Gluconate 5-dehydrogenase
| CENTER ID: | BuceA.00010.x |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00010.x.B1.GE36352 | Full length( BuceA.00010.x ) | 1 | 257 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00010.x.B1.PW37241 | Full length( BuceA.00010.x ) | 1 | 257 |
Structures
5U9P
DEPOSITED: 12/17/2016
DETERMINATION: XRay
CLONE: BuceA.00010.x.B1.GE36352
PROTEIN: BuceA.00010.x.B1.PW37241
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126618 |
| PATRIC ID: | fig|216591.5.peg.3513 |
| RefSeq: | YP_002232488.1 |
| UniProt: | B4EEX4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTHALDRFRL DGRRALITGS GRGIGLTLAR GLAEAGAAIV INDRNEEKAA TLARRFRDEG FAADHAVFDV AEHAQVRAAI DEFEARVGAI DILVNNAGIQ RRAPLDAFEP DDWHALMRVN LDGVFNVAQA VARHMIARGH GKIINICSVQ SELARPTIAP YAATKGAVRM LTKGMCADWA RYGIQANGLA PGYFETELNR ALVDDAAFSD WLCKRTPAGR WGQVDELCGA AIFLASAASD FVNGQTLFVD GGLTSAV
NT Sequence
ATGACCCATG CGCTTGACCG GTTCCGCCTC GACGGCCGCC GCGCGCTGAT TACCGGTTCC GGCCGCGGGA TCGGACTGAC GCTCGCCCGC GGGCTCGCGG AAGCCGGCGC GGCGATCGTC ATCAACGATC GCAATGAAGA GAAGGCCGCG ACGCTCGCGC GCCGGTTTCG CGACGAAGGC TTCGCGGCCG ACCATGCGGT GTTCGACGTG GCCGAGCACG CGCAGGTCCG TGCGGCGATC GACGAGTTCG AGGCGCGCGT CGGCGCGATC GACATCCTCG TGAACAACGC GGGCATCCAG CGCCGCGCGC CGCTCGACGC TTTCGAGCCG GACGACTGGC ATGCGCTGAT GCGCGTGAAT CTCGACGGCG TGTTCAACGT CGCGCAGGCC GTCGCGCGGC ACATGATCGC GCGTGGCCAC GGCAAGATCA TCAACATCTG CTCGGTGCAG AGCGAGCTCG CGCGGCCCAC GATCGCGCCG TATGCGGCGA CCAAGGGCGC GGTGCGGATG CTGACCAAAG GGATGTGCGC CGACTGGGCG CGCTACGGCA TCCAGGCGAA CGGCCTCGCA CCCGGCTATT TCGAAACCGA ACTGAATCGC GCGCTGGTCG ACGACGCGGC GTTTTCTGAC TGGCTGTGCA AGCGTACGCC CGCCGGCCGC TGGGGGCAGG TCGACGAGCT GTGCGGCGCG GCGATCTTCC TCGCGTCGGC CGCGTCCGAT TTCGTGAACG GCCAGACGCT GTTCGTCGAC GGCGGGCTGA CCAGTGCCGT C
Details for BuceA.00010.x.B1.GE36352
| HARVESTED ON: | 10/23/2012 |
| SEQUENCED ON: | 10/26/2012 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT HALDRFRLDG RRALITGSGR GIGLTLARGL AEAGAAIVIN DRNEEKAATL ARRFRDEGFA ADHAVFDVAE HAQVRAAIDE FEARVGAIDI LVNNAGIQRR APLDAFEPDD WHALMRVNLD GVFNVAQAVA RHMIARGHGK IINICSVQSE LARPTIAPYA ATKGAVRMLT KGMCADWARY GIQANGLAPG YFETELNRAL VDDAAFSDWL CKRTPAGRWG QVDELCGAAI FLASAASDFV NGQTLFVDGG LTSAV
Validated NT Sequence
atcttactca cttagacggc actggtcagc ccgccgtcga cgaacagcgt ctggccgttc acgaaatcgg acgcggccga cgcgaggaag atcgccgcgc cgcacagctc gtcgacctgc ccccagcggc cggcgggcgt acgcttgcac agccagtcag aaaacgccgc gtcgtcgacc agcgcgcgat tcagttcggt ttcgaaatag ccgggtgcga ggccgttcgc ctggatgccg tagcgcgccc agtcggcgca catccctttg gtcagcatcc gcaccgcgcc cttggtcgcc gcatacggcg cgatcgtggg ccgcgcgagc tcgctctgca ccgagcagat gttgatgatc ttgccgtggc cacgcgcgat catgtgccgc gcgacggcct gcgcgacgtt gaacacgccg tcgagattca cgcgcatcag cgcatgccag tcgtccggct cgaaagcgtc gagcggcgcg cggcgctgga tgcccgcgtt gttcacgagg atgtcgatcg cgccgacgcg cgcctcgaac tcgtcgatcg ccgcacggac ctgcgcgtgc tcggccacgt cgaacaccgc atggtcggcc gcgaagcctt cgtcgcgaaa ccggcgcgcg agcgtcgcgg ccttctcttc attgcgatcg ttgatgacga tcgccgcgcc ggcttccgcg agcccgcggg cgagcgtcag tccgatcccg cggccggaac cggtaatcag cgcgcggcgg ccgtcgaggc ggaaccggtc aagcgcatgg gtcatatggt ggtggtggtg gtgagccatg gta
Expected Protein Sequence
MAHHHHHHMT HALDRFRLDG RRALITGSGR GIGLTLARGL AEAGAAIVIN DRNEEKAATL ARRFRDEGFA ADHAVFDVAE HAQVRAAIDE FEARVGAIDI LVNNAGIQRR APLDAFEPDD WHALMRVNLD GVFNVAQAVA RHMIARGHGK IINICSVQSE LARPTIAPYA ATKGAVRMLT KGMCADWARY GIQANGLAPG YFETELNRAL VDDAAFSDWL CKRTPAGRWG QVDELCGAAI FLASAASDFV NGQTLFVDGG LTSAV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccc atgcgcttga ccggttccgc ctcgacggcc gccgcgcgct gattaccggt tccggccgcg ggatcggact gacgctcgcc cgcgggctcg cggaagccgg cgcggcgatc gtcatcaacg atcgcaatga agagaaggcc gcgacgctcg cgcgccggtt tcgcgacgaa ggcttcgcgg ccgaccatgc ggtgttcgac gtggccgagc acgcgcaggt ccgtgcggcg atcgacgagt tcgaggcgcg cgtcggcgcg atcgacatcc tcgtgaacaa cgcgggcatc cagcgccgcg cgccgctcga cgctttcgag ccggacgact ggcatgcgct gatgcgcgtg aatctcgacg gcgtgttcaa cgtcgcgcag gccgtcgcgc ggcacatgat cgcgcgtggc cacggcaaga tcatcaacat ctgctcggtg cagagcgagc tcgcgcggcc cacgatcgcg ccgtatgcgg cgaccaaggg cgcggtgcgg atgctgacca aagggatgtg cgccgactgg gcgcgctacg gcatccaggc gaacggcctc gcacccggct atttcgaaac cgaactgaat cgcgcgctgg tcgacgacgc ggcgttttct gactggctgt gcaagcgtac gcccgccggc cgctgggggc aggtcgacga gctgtgcggc gcggcgatct tcctcgcgtc ggccgcgtcc gatttcgtga acggccagac gctgttcgtc gacggcgggc tgaccagtgc cgtctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BuceA.00010.x.B1.PW37241
| PURIFICATION DATe: | 2/4/2013 |
| CONCENTRATION: | 47.7mg/ml |
| OBSERVED MW: | 30kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT HALDRFRLDG RRALITGSGR GIGLTLARGL AEAGAAIVIN DRNEEKAATL ARRFRDEGFA ADHAVFDVAE HAQVRAAIDE FEARVGAIDI LVNNAGIQRR APLDAFEPDD WHALMRVNLD GVFNVAQAVA RHMIARGHGK IINICSVQSE LARPTIAPYA ATKGAVRMLT KGMCADWARY GIQANGLAPG YFETELNRAL VDDAAFSDWL CKRTPAGRWG QVDELCGAAI FLASAASDFV NGQTLFVDGG LTSAV
Validated NT Sequence
atcttactca cttagacggc actggtcagc ccgccgtcga cgaacagcgt ctggccgttc acgaaatcgg acgcggccga cgcgaggaag atcgccgcgc cgcacagctc gtcgacctgc ccccagcggc cggcgggcgt acgcttgcac agccagtcag aaaacgccgc gtcgtcgacc agcgcgcgat tcagttcggt ttcgaaatag ccgggtgcga ggccgttcgc ctggatgccg tagcgcgccc agtcggcgca catccctttg gtcagcatcc gcaccgcgcc cttggtcgcc gcatacggcg cgatcgtggg ccgcgcgagc tcgctctgca ccgagcagat gttgatgatc ttgccgtggc cacgcgcgat catgtgccgc gcgacggcct gcgcgacgtt gaacacgccg tcgagattca cgcgcatcag cgcatgccag tcgtccggct cgaaagcgtc gagcggcgcg cggcgctgga tgcccgcgtt gttcacgagg atgtcgatcg cgccgacgcg cgcctcgaac tcgtcgatcg ccgcacggac ctgcgcgtgc tcggccacgt cgaacaccgc atggtcggcc gcgaagcctt cgtcgcgaaa ccggcgcgcg agcgtcgcgg ccttctcttc attgcgatcg ttgatgacga tcgccgcgcc ggcttccgcg agcccgcggg cgagcgtcag tccgatcccg cggccggaac cggtaatcag cgcgcggcgg ccgtcgaggc ggaaccggtc aagcgcatgg gtcatatggt ggtggtggtg gtgagccatg gta
Expressed Protein Sequence
MAHHHHHHMT HALDRFRLDG RRALITGSGR GIGLTLARGL AEAGAAIVIN DRNEEKAATL ARRFRDEGFA ADHAVFDVAE HAQVRAAIDE FEARVGAIDI LVNNAGIQRR APLDAFEPDD WHALMRVNLD GVFNVAQAVA RHMIARGHGK IINICSVQSE LARPTIAPYA ATKGAVRMLT KGMCADWARY GIQANGLAPG YFETELNRAL VDDAAFSDWL CKRTPAGRWG QVDELCGAAI FLASAASDFV NGQTLFVDGG LTSAV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCC ATGCGCTTGA CCGGTTCCGC CTCGACGGCC GCCGCGCGCT GATTACCGGT TCCGGCCGCG GGATCGGACT GACGCTCGCC CGCGGGCTCG CGGAAGCCGG CGCGGCGATC GTCATCAACG ATCGCAATGA AGAGAAGGCC GCGACGCTCG CGCGCCGGTT TCGCGACGAA GGCTTCGCGG CCGACCATGC GGTGTTCGAC GTGGCCGAGC ACGCGCAGGT CCGTGCGGCG ATCGACGAGT TCGAGGCGCG CGTCGGCGCG ATCGACATCC TCGTGAACAA CGCGGGCATC CAGCGCCGCG CGCCGCTCGA CGCTTTCGAG CCGGACGACT GGCATGCGCT GATGCGCGTG AATCTCGACG GCGTGTTCAA CGTCGCGCAG GCCGTCGCGC GGCACATGAT CGCGCGTGGC CACGGCAAGA TCATCAACAT CTGCTCGGTG CAGAGCGAGC TCGCGCGGCC CACGATCGCG CCGTATGCGG CGACCAAGGG CGCGGTGCGG ATGCTGACCA AAGGGATGTG CGCCGACTGG GCGCGCTACG GCATCCAGGC GAACGGCCTC GCACCCGGCT ATTTCGAAAC CGAACTGAAT CGCGCGCTGG TCGACGACGC GGCGTTTTCT GACTGGCTGT GCAAGCGTAC GCCCGCCGGC CGCTGGGGGC AGGTCGACGA GCTGTGCGGC GCGGCGATCT TCCTCGCGTC GGCCGCGTCC GATTTCGTGA ACGGCCAGAC GCTGTTCGTC GACGGCGGGC TGACCAGTGC CGTCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT