BuceA.00010.v
Short chain dehydrogenase
| CENTER ID: | BuceA.00010.v |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00010.v.B2.GE36350 | Mature protein( BuceA.00010.v ) | 28 | 328 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5JYD
DEPOSITED: 5/13/2016
DETERMINATION: XRay
CLONE: BuceA.00010.v.B2.GE36350
PROTEIN: BuceA.00010.v.B2.PS01749
5U8P
DEPOSITED: 12/14/2016
DETERMINATION: XRay
CLONE: BuceA.00010.v.B2.GE36350
PROTEIN: BuceA.00010.v.B2.PS01749
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128170 |
| PATRIC ID: | fig|216591.5.peg.1247 |
| RefSeq: | YP_002230344.1 |
| UniProt: | B4EEE3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVSRRQVIG NLGALAAMSA LGPSTAHAVD APPAAQGLTN PVDLYPKPPF PHQVQAPPGL ASRMQPRPDH GEQSYRGRGR LVGRKTLVTG GDSGIGRAAA IAFAREGADV AIGYLPVEES DAREVVALIR AAGRQAVALP GDIRDETFCQ RLVARAAEAL GGLDILVNNA ARQQALDSIG EMTTEHFDAT VKTNLYGMFW ITKAAIPHLP PGASIINTTS VQAVRASANL LDYATTKAGI IAFTRSLAKQ LGPRGIRVNA VAPGPYWTPL QSSGGQPPET VVNYAAGSPY GRPGQPAEIA PLYVALAASE TSYANGQVWG ADGGLGIF
NT Sequence
ATGCGCGTGT CGCGCCGACA GGTCATCGGC AATCTTGGCG CACTGGCCGC GATGTCCGCA CTCGGCCCGT CCACCGCGCA TGCCGTCGAC GCGCCGCCCG CCGCGCAGGG CCTGACGAAT CCGGTCGATC TGTACCCGAA GCCGCCGTTC CCGCATCAGG TCCAGGCGCC GCCGGGACTC GCGAGCCGGA TGCAGCCGCG CCCCGACCAC GGCGAGCAAA GCTATCGCGG CCGTGGCCGG CTCGTCGGCC GCAAGACGCT CGTCACCGGC GGCGACTCCG GCATCGGCCG GGCAGCGGCC ATCGCGTTCG CACGCGAAGG GGCGGATGTC GCGATCGGCT ACCTGCCTGT GGAGGAATCC GACGCGCGGG AAGTCGTTGC GCTGATCCGG GCAGCGGGAC GCCAAGCCGT CGCGCTGCCG GGCGACATCC GCGACGAAAC GTTCTGCCAG CGACTGGTCG CACGCGCGGC GGAAGCACTC GGCGGCCTCG ACATTCTCGT CAACAACGCC GCCCGACAAC AGGCGCTCGA TTCGATCGGC GAGATGACGA CCGAGCACTT CGATGCGACG GTCAAGACCA ATCTTTACGG GATGTTCTGG ATCACGAAGG CGGCGATCCC GCATCTTCCG CCCGGCGCGT CGATCATCAA CACCACGTCG GTCCAGGCGG TCAGGGCGAG CGCCAACCTG CTCGACTATG CGACGACGAA AGCCGGCATC ATCGCGTTCA CGCGATCGCT CGCGAAACAG CTCGGGCCGC GCGGCATTCG CGTCAATGCG GTCGCGCCCG GCCCTTACTG GACGCCGCTG CAATCGAGCG GCGGTCAGCC ACCGGAGACG GTCGTCAACT ATGCGGCGGG CTCGCCGTAC GGCAGGCCCG GGCAACCGGC GGAAATCGCC CCGCTGTATG TCGCGCTCGC GGCGTCGGAA ACGAGCTATG CGAACGGGCA GGTCTGGGGC GCGGACGGCG GGCTGGGGAT TTTC
Details for BuceA.00010.v.B2.GE36350
| HARVESTED ON: | 10/23/2012 |
| SEQUENCED ON: | 10/26/2012 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA VDAPPAAQGL TNPVDLYPKP PFPHQVQAPP GLASRMQPRP DHGEQSYRGR GRLVGRKTLV TGGDSGIGRA AAIAFAREGA DVAIGYLPVE ESDAREVVAL IRAAGRQAVA LPGDIRDETF CQRLVARAAE ALGGLDILVN NAARQQALDS IGEMTTEHFD ATVKTNLYGM FWITKAAIPH LPPGASIINT TSVQAVRASA NLLDYATTKA GIIAFTRSLA KQLGPRGIRV NAVAPGPYWT PLQSSGGQPP ETVVNYAAGS PYGRPGQPAE IAPLYVALAA SETSYANGQV WGADGGLGIF
Validated NT Sequence
tactcactta gaaaatcccc agcccgccgt ccgcgcccca gacctgcccg ttcgcatagc tcgtttccga cgccgcgagc gcgacataca gcggggcgat ttccgccggt tgcccgggcc tgccgtacgg cgagcccgcc gcatagttga cgaccgtctc cggtggctga ccgccgctcg attgcagcgg cgtccagtaa gggccgggcg cgaccgcatt gacgcgaatg ccgcgcggcc cgagctgttt cgcgagcgat cgcgtgaacg cgatgatgcc ggctttcgtc gtcgcatagt cgagcaggtt ggcgctcgcc ctgaccgcct ggaccgacgt ggtgttgatg atcgacgcgc cgggcggaag atgcgggatc gccgccttcg tgatccagaa catcccgtaa agattggtct tgaccgtcgc atcgaagtgc tcggtcgtca tctcgccgat cgaatcgagc gcctgttgtc gggcggcgtt gttgacgaga atgtcgaggc cgccgagtgc ttccgccgcg cgtgcgacca gtcgctggca gaacgtttcg tcgcggatgt cgcccggcag cgcgacggct tggcgtcccg ctgcccggat cagcgcaacg acttcccgcg cgtcggattc ctccacaggc aggtagccga tcgcgacatc cgccccttcg cgtgcgaacg cgatggccgc tgcccggccg atgccggagt cgccgccggt gacgagcgtc ttgcggccga cgagccggcc acggccgcga tagctttgct cgccgtggtc ggggcgcggc tgcatccggc tcgcgagtcc cggcggcgcc tggacctgat gcgggaacgg cggcttcggg tacagatcga ccggattcgt caggccctgc gcggcgggcg gcgcgtcgac ggccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMA VDAPPAAQGL TNPVDLYPKP PFPHQVQAPP GLASRMQPRP DHGEQSYRGR GRLVGRKTLV TGGDSGIGRA AAIAFAREGA DVAIGYLPVE ESDAREVVAL IRAAGRQAVA LPGDIRDETF CQRLVARAAE ALGGLDILVN NAARQQALDS IGEMTTEHFD ATVKTNLYGM FWITKAAIPH LPPGASIINT TSVQAVRASA NLLDYATTKA GIIAFTRSLA KQLGPRGIRV NAVAPGPYWT PLQSSGGQPP ETVVNYAAGS PYGRPGQPAE IAPLYVALAA SETSYANGQV WGADGGLGIF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg tcgacgcgcc gcccgccgcg cagggcctga cgaatccggt cgatctgtac ccgaagccgc cgttcccgca tcaggtccag gcgccgccgg gactcgcgag ccggatgcag ccgcgccccg accacggcga gcaaagctat cgcggccgtg gccggctcgt cggccgcaag acgctcgtca ccggcggcga ctccggcatc ggccgggcag cggccatcgc gttcgcacgc gaaggggcgg atgtcgcgat cggctacctg cctgtggagg aatccgacgc gcgggaagtc gttgcgctga tccgggcagc gggacgccaa gccgtcgcgc tgccgggcga catccgcgac gaaacgttct gccagcgact ggtcgcacgc gcggcggaag cactcggcgg cctcgacatt ctcgtcaaca acgccgcccg acaacaggcg ctcgattcga tcggcgagat gacgaccgag cacttcgatg cgacggtcaa gaccaatctt tacgggatgt tctggatcac gaaggcggcg atcccgcatc ttccgcccgg cgcgtcgatc atcaacacca cgtcggtcca ggcggtcagg gcgagcgcca acctgctcga ctatgcgacg acgaaagccg gcatcatcgc gttcacgcga tcgctcgcga aacagctcgg gccgcgcggc attcgcgtca atgcggtcgc gcccggccct tactggacgc cgctgcaatc gagcggcggt cagccaccgg agacggtcgt caactatgcg gcgggctcgc cgtacggcag gcccgggcaa ccggcggaaa tcgccccgct gtatgtcgcg ctcgcggcgt cggaaacgag ctatgcgaac gggcaggtct ggggcgcgga cggcgggctg gggattttct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat