SSGCID: Target Page

BuceA.00010.u

Short chain dehydrogenase

CENTER ID:	BuceA.00010.u
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00010.u.B1.GE36349	Full length( BuceA.00010.u )		1	263

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2354
RefSeq:	YP_002231382.1
UniProt:	B4EDY5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLLKDKIVVI SGIGPGLGVK LAVEAAREGA RGVVVAARTM EKLDDADARI RALDVDCAVL KVQTDITDRA QCRQLATRAV ERFGRIDALV NSAFVHGTFP EPVEQADLDG WRAVFETNVF GTMALTQEVV PHMKRQQRGA IVMINTQATR KPFAGEGGYA VSKGALAVAA KYLARELGVH GIRANSIHMG WMWGVPTQTY FRQAAADYGM TEAQIVAPIA SNIALAKLPT DDDCARAALF LASDYANAVT GATLDANGGD FMP

NT Sequence

ATGTTGCTGA AGGACAAGAT CGTCGTGATT TCCGGAATCG GGCCGGGACT CGGCGTGAAG CTCGCGGTCG AAGCCGCGCG CGAGGGCGCA CGCGGCGTGG TGGTCGCGGC GCGCACGATG GAAAAGCTCG ACGACGCCGA CGCGCGAATC CGTGCACTCG ATGTCGATTG CGCGGTGCTG AAGGTGCAGA CCGACATCAC CGATCGGGCA CAATGCCGGC AGCTCGCGAC GCGGGCCGTC GAGCGCTTCG GCCGGATCGA CGCGCTCGTG AACAGCGCGT TCGTGCATGG GACGTTTCCG GAGCCGGTGG AGCAAGCCGA CCTCGACGGC TGGCGCGCGG TGTTCGAGAC CAACGTGTTC GGTACGATGG CGCTCACGCA GGAAGTCGTG CCGCACATGA AACGGCAGCA GCGCGGCGCG ATCGTGATGA TCAACACGCA GGCGACCCGC AAGCCGTTTG CAGGGGAGGG CGGCTACGCT GTATCGAAGG GCGCGCTCGC GGTTGCGGCA AAATACCTGG CGCGCGAACT CGGCGTGCAC GGCATTCGCG CGAACAGCAT CCACATGGGC TGGATGTGGG GCGTGCCGAC GCAGACCTAT TTCCGGCAGG CGGCCGCCGA CTACGGGATG ACGGAGGCGC AGATCGTTGC GCCGATCGCG TCGAACATCG CGCTCGCGAA GCTGCCGACC GACGACGATT GCGCGCGTGC GGCGCTGTTC CTCGCGTCCG ACTACGCGAA CGCGGTGACG GGCGCGACGC TCGACGCGAA CGGCGGCGAT TTCATGCCT

Details for BuceA.00010.u.B1.GE36349

HARVESTED ON:	10/23/2012
SEQUENCED ON:	10/26/2012
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML LKDKIVVISG IGPGLGVKLA VEAAREGARG VVVAARTMEK LDDADARIRA LDVDCAVLKV QTDITDRAQC RQLATRAVER FGRIDALVNS AFVHGTFPEP VEQADLDGWR AVFETNVFGT MALTQEVVPH MKRQQRGAIV MINTQATRKP FAGEGGYAVS KGALAVAAKY LARELGVHGI RANSIHMGWM WGVPTQTYFR QAAADYGMTE AQIVAPIASN IALAKLPTDD DCARAALFLA SDYANAVTGA TLDANGGDFM P

Validated NT Sequence

atcttactca cttaaggcat gaaatcgccg ccgttcgcgt cgagcgtcgc gcccgtcacc gcgttcgcgt agtcggacgc gaggaacagc gccgcacgcg cgcaatcgtc gtcggtcggc agcttcgcga gcgcgatgtt cgacgcgatc ggcgcaacga tctgcgcctc cgtcatcccg tagtcggcgg ccgcctgccg gaaataggtc tgcgtcggca cgccccacat ccagcccatg tggatgctgt tcgcgcgaat gccgtgcacg ccgagttcgc gcgccaggta ttttgccgca accgcgagcg cgcccttcga tacagcgtag ccgccctccc ctgcaaacgg cttgcgggtc gcctgcgtgt tgatcatcac gatcgcgccg cgctgctgcc gtttcatgtg cggcacgact tcctgcgtga gcgccatcgt accgaacacg ttggtctcga acaccgcgcg ccagccgtcg aggtcggctt gctccaccgg ctccggaaac gtcccatgca cgaacgcgct gttcacgagc gcgtcgatcc ggccgaagcg ctcgacggcc cgcgtcgcga gctgccggca ttgtgcccga tcggtgatgt cggtctgcac cttcagcacc gcgcaatcga catcgagtgc acggattcgc gcgtcggcgt cgtcgagctt ttccatcgtg cgcgccgcga ccaccacgcc gcgtgcgccc tcgcgcgcgg cttcgaccgc gagcttcacg ccgagtcccg gcccgattcc ggaaatcacg acgatcttgt ccttcagcaa catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgc tgaaggacaa gatcgtcgtg atttccggaa tcgggccggg actcggcgtg aagctcgcgg tcgaagccgc gcgcgagggc gcacgcggcg tggtggtcgc ggcgcgcacg atggaaaagc tcgacgacgc cgacgcgcga atccgtgcac tcgatgtcga ttgcgcggtg ctgaaggtgc agaccgacat caccgatcgg gcacaatgcc ggcagctcgc gacgcgggcc gtcgagcgct tcggccggat cgacgcgctc gtgaacagcg cgttcgtgca tgggacgttt ccggagccgg tggagcaagc cgacctcgac ggctggcgcg cggtgttcga gaccaacgtg ttcggtacga tggcgctcac gcaggaagtc gtgccgcaca tgaaacggca gcagcgcggc gcgatcgtga tgatcaacac gcaggcgacc cgcaagccgt ttgcagggga gggcggctac gctgtatcga agggcgcgct cgcggttgcg gcaaaatacc tggcgcgcga actcggcgtg cacggcattc gcgcgaacag catccacatg ggctggatgt ggggcgtgcc gacgcagacc tatttccggc aggcggccgc cgactacggg atgacggagg cgcagatcgt tgcgccgatc gcgtcgaaca tcgcgctcgc gaagctgccg accgacgacg attgcgcgcg tgcggcgctg ttcctcgcgt ccgactacgc gaacgcggtg acgggcgcga cgctcgacgc gaacggcggc gatttcatgc cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat