SSGCID: Target Page

BuceA.00010.s

Putative capsular polysaccharide biosynthesis dehydrogenase/reductase protein

CENTER ID:	BuceA.00010.s
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00010.s.B1.GE36347	Full length( BuceA.00010.s )		1	264

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00010.s.B1.PS01769	Full length( BuceA.00010.s )		1	264

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.3347
RefSeq:	YP_002232333.1
UniProt:	B4EDE1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGSLAQPRNV VITGASAGIG RALALAYARP GVVLGLVGRD IERLDASASE CRTRGAVVVT GQFDVRDADS AKVWLDGFDD AHPIDLLIAN AGVASTLATA NDWEGLERTA RVVDTNFYGA LHAVLPVVDR MRGRQQGHVV MVASLAALRG MAISPAYCAS KSAIKAYADS VRPLLKRNGI RMSVVLPGFV ETSMSDVFPS DKPFLWTANK AASYIRRKLA AGRAEVAFPG LLAFGMRLLP LLPSSLADAI LDKLSYFPRE EDIK

NT Sequence

ATGGGTAGCC TTGCCCAACC ACGTAATGTC GTTATCACTG GCGCAAGCGC CGGTATCGGC CGTGCGCTTG CGCTGGCTTA CGCCCGGCCG GGTGTCGTGC TCGGTCTCGT CGGCCGCGAT ATCGAGCGGC TCGACGCAAG TGCCTCGGAA TGCCGTACTC GCGGTGCTGT TGTTGTTACG GGGCAGTTTG ACGTGCGCGA TGCCGACAGT GCAAAAGTGT GGCTTGACGG CTTCGACGAC GCCCATCCGA TTGATCTGCT GATCGCGAAC GCGGGAGTTG CGAGTACGCT TGCCACGGCG AACGACTGGG AGGGGCTGGA GCGCACGGCG CGGGTGGTCG ACACGAATTT TTATGGCGCC CTGCATGCCG TGCTGCCTGT CGTCGACCGG ATGCGCGGAC GACAGCAGGG CCATGTCGTT ATGGTCGCGT CGCTGGCCGC GCTGCGCGGG ATGGCGATCT CGCCGGCCTA TTGTGCGAGC AAGTCGGCGA TCAAGGCGTA TGCCGATTCC GTCCGGCCGT TGCTCAAGCG CAATGGCATC CGGATGTCGG TGGTCTTGCC CGGATTCGTT GAAACGTCAA TGAGCGACGT ATTTCCCAGC GACAAGCCAT TTCTCTGGAC AGCGAACAAG GCGGCGTCAT ATATCCGTCG CAAACTCGCA GCCGGGCGCG CCGAAGTTGC GTTTCCCGGT CTGCTGGCGT TCGGAATGCG CTTGTTGCCG CTGTTGCCGT CGTCACTCGC TGACGCCATT CTCGACAAGC TGTCTTATTT TCCCAGGGAG GAAGACATCA AG

Details for BuceA.00010.s.B1.GE36347

HARVESTED ON:	10/23/2012
SEQUENCED ON:	10/26/2012
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	84
PERCENT COVERAGE:	104

Validated AA Sequence

MAHHHHHHMG SLAQPRNVVI TGASAGIGRA LALAYARPGV VLGLVGRDIE RLDASASECR TRGAVVVTGQ FDVRDADSAK VWLDGFDDAH PIDLLIANAG VASTLATAND WEGLERTARV VDTNFYGALH AVLPVVDRMR GRQQGHVVMV ASLAALRGMA ISPAYCASKS AIKAYADSVR PLLKRNGIRM SVXLPGFXET SMSDVFPSDK PFLWTAXKXA SYXRRXXQPG APNCVSXSAG VRNALVAXVA VXTXXXXXXX XLIXPGRKTS XXSXXGXXLX XAPX

Validated NT Sequence

ttntnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgggtagc cttgcccaac cacgtaatgt cgttatcact ggcgcaagcg ccggtatcgg ccgtgcgctt gcgctggctt acgcccggcc gggtgtcgtg ctcggtctcg tcggccgcga tatcgagcgg ctcgacgcaa gtgcctcgga atgccgtact cgcggtgctg ttgttgttac ggggcagttt gacgtgcgcg atgccgacag tgcaaaagtg tggcttgacg gcttcgacga cgcccatccg attgatctgc tgatcgcgaa cgcgggagtt gcgagtacgc ttgccacggc gaacgactgg gaggggctgg agcgcacggc gcgggtggtc gacacgaatt tttatggcgc cctgcatgcc gtgctgcctg tcgtcgaccg gatgcgcgga cgacagcagg gccatgtcgt tatggtcgcg tcgctggccg cgctgcgcgg gatggcgatc tcgccggcct attgtgcgag caagtcggcg atcaaggcgt atgccgattc cgtccggccg ttgctcaagc gcaatggcat ccggatgtcg gtggncttgc ccggattcnt tgaaacgtcn atgagcgacg tatttcccag cgacaagcca tttctctgga cagcgaanaa ggnggcgtca tatatncgtc gcanctnnca gccnggcgcg ccgaattgcg tttcccngtc tgctggcgtt cggaatgcnc ttgttgccnc tgttgccgtc gncactcnnt gnnncnttnn nnanannngt cttatttnnc cngggaggaa gacatcanna nngagtanan nagganncnn nctgcnanna gccccnnnng

Expected Protein Sequence

MAHHHHHHMG SLAQPRNVVI TGASAGIGRA LALAYARPGV VLGLVGRDIE RLDASASECR TRGAVVVTGQ FDVRDADSAK VWLDGFDDAH PIDLLIANAG VASTLATAND WEGLERTARV VDTNFYGALH AVLPVVDRMR GRQQGHVVMV ASLAALRGMA ISPAYCASKS AIKAYADSVR PLLKRNGIRM SVVLPGFVET SMSDVFPSDK PFLWTANKAA SYIRRKLAAG RAEVAFPGLL AFGMRLLPLL PSSLADAILD KLSYFPREED IK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggta gccttgccca accacgtaat gtcgttatca ctggcgcaag cgccggtatc ggccgtgcgc ttgcgctggc ttacgcccgg ccgggtgtcg tgctcggtct cgtcggccgc gatatcgagc ggctcgacgc aagtgcctcg gaatgccgta ctcgcggtgc tgttgttgtt acggggcagt ttgacgtgcg cgatgccgac agtgcaaaag tgtggcttga cggcttcgac gacgcccatc cgattgatct gctgatcgcg aacgcgggag ttgcgagtac gcttgccacg gcgaacgact gggaggggct ggagcgcacg gcgcgggtgg tcgacacgaa tttttatggc gccctgcatg ccgtgctgcc tgtcgtcgac cggatgcgcg gacgacagca gggccatgtc gttatggtcg cgtcgctggc cgcgctgcgc gggatggcga tctcgccggc ctattgtgcg agcaagtcgg cgatcaaggc gtatgccgat tccgtccggc cgttgctcaa gcgcaatggc atccggatgt cggtggtctt gcccggattc gttgaaacgt caatgagcga cgtatttccc agcgacaagc catttctctg gacagcgaac aaggcggcgt catatatccg tcgcaaactc gcagccgggc gcgccgaagt tgcgtttccc ggtctgctgg cgttcggaat gcgcttgttg ccgctgttgc cgtcgtcact cgctgacgcc attctcgaca agctgtctta ttttcccagg gaggaagaca tcaagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for BuceA.00010.s.B1.PS01769

PURIFICATION DATe:	3/13/2013
CONCENTRATION:	31mg/ml
OBSERVED MW:	30kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	84
PERCENT COVERAGE:	104

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG SLAQPRNVVI TGASAGIGRA LALAYARPGV VLGLVGRDIE RLDASASECR TRGAVVVTGQ FDVRDADSAK VWLDGFDDAH PIDLLIANAG VASTLATAND WEGLERTARV VDTNFYGALH AVLPVVDRMR GRQQGHVVMV ASLAALRGMA ISPAYCASKS AIKAYADSVR PLLKRNGIRM SVVLPGFVET SMSDVFPSDK PFLWTANKAA SYIRRKLAAG RAEVAFPGLL AFGMRLLPLL PSSLADAILD KLSYFPREED IK

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGTA GCCTTGCCCA ACCACGTAAT GTCGTTATCA CTGGCGCAAG CGCCGGTATC GGCCGTGCGC TTGCGCTGGC TTACGCCCGG CCGGGTGTCG TGCTCGGTCT CGTCGGCCGC GATATCGAGC GGCTCGACGC AAGTGCCTCG GAATGCCGTA CTCGCGGTGC TGTTGTTGTT ACGGGGCAGT TTGACGTGCG CGATGCCGAC AGTGCAAAAG TGTGGCTTGA CGGCTTCGAC GACGCCCATC CGATTGATCT GCTGATCGCG AACGCGGGAG TTGCGAGTAC GCTTGCCACG GCGAACGACT GGGAGGGGCT GGAGCGCACG GCGCGGGTGG TCGACACGAA TTTTTATGGC GCCCTGCATG CCGTGCTGCC TGTCGTCGAC CGGATGCGCG GACGACAGCA GGGCCATGTC GTTATGGTCG CGTCGCTGGC CGCGCTGCGC GGGATGGCGA TCTCGCCGGC CTATTGTGCG AGCAAGTCGG CGATCAAGGC GTATGCCGAT TCCGTCCGGC CGTTGCTCAA GCGCAATGGC ATCCGGATGT CGGTGGTCTT GCCCGGATTC GTTGAAACGT CAATGAGCGA CGTATTTCCC AGCGACAAGC CATTTCTCTG GACAGCGAAC AAGGCGGCGT CATATATCCG TCGCAAACTC GCAGCCGGGC GCGCCGAAGT TGCGTTTCCC GGTCTGCTGG CGTTCGGAAT GCGCTTGTTG CCGCTGTTGC CGTCGTCACT CGCTGACGCC ATTCTCGACA AGCTGTCTTA TTTTCCCAGG GAGGAAGACA TCAAGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT