SSGCID: Target Page

BuceA.00010.q

Putative acetoin(Diacetyl) reductase

CENTER ID:	BuceA.00010.q
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00010.q.B1.GE36345	Full length( BuceA.00010.q )		1	258

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

4WEO

DEPOSITED: 9/10/2014

DETERMINATION: XRay

Diffraction Image

CLONE: BuceA.00010.q.B1.GE36345

PROTEIN: BuceA.00010.q.B1.PS01731

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126618
PATRIC ID:	fig\|216591.5.peg.1986
RefSeq:	YP_002231040.1
UniProt:	B4EAZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTAPLEGQVA IVTGGARGIG RGIALTLAGA GANILLADLL DDALDATARE VRALGRRAAI AKVDVTQAAQ VDAMVAQALA DLGGLDILVN CAGVISIHPV AELTERDWDF VMNVNAKGTF LGCRAALAHL KAQGRGRIIN VASIAGKEGF PNLAHYSASK FAVVGFTNAL AKELARDGVT VNAICPGIVR TYMWDRLSDE WKTDGESVEQ SWQRHQLTLI PQGRAQTPED MGRLALFFAT MDNVTGQAVN VDGGFTFH

NT Sequence

ATGACAGCCC CTCTCGAAGG ACAGGTCGCC ATCGTCACCG GCGGCGCGCG CGGCATCGGC CGTGGCATCG CGCTGACGCT CGCCGGCGCC GGCGCCAACA TCCTGCTGGC CGACCTGCTC GACGACGCGC TCGACGCCAC CGCGCGCGAG GTGCGTGCGC TCGGCCGCCG CGCGGCGATC GCGAAGGTCG ACGTCACGCA GGCCGCGCAG GTCGACGCGA TGGTCGCGCA GGCGCTCGCC GACCTCGGCG GGCTCGACAT CCTCGTGAAC TGCGCGGGCG TGATCAGCAT TCATCCGGTC GCCGAGCTGA CCGAGCGCGA CTGGGATTTC GTGATGAACG TGAACGCGAA GGGCACGTTC CTCGGCTGCC GGGCGGCGCT TGCCCACCTG AAGGCGCAGG GGCGCGGCCG GATCATCAAC GTCGCGTCGA TCGCGGGCAA GGAAGGCTTT CCGAATCTCG CGCATTACAG CGCATCGAAA TTCGCGGTCG TGGGCTTCAC CAACGCGCTG GCGAAGGAAC TCGCGCGCGA CGGCGTGACC GTCAACGCGA TCTGCCCCGG CATCGTCCGT ACCTACATGT GGGACCGGCT GTCCGACGAA TGGAAGACCG ACGGCGAATC GGTCGAGCAG TCGTGGCAGC GGCACCAGTT GACGCTGATT CCGCAGGGCC GAGCGCAGAC GCCGGAGGAC ATGGGCCGGC TCGCGCTGTT CTTCGCGACG ATGGACAACG TGACGGGCCA GGCCGTGAAC GTCGACGGCG GCTTCACGTT CCAC

Details for BuceA.00010.q.B1.GE36345

HARVESTED ON:	10/23/2012
SEQUENCED ON:	10/26/2012
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT APLEGQVAIV TGGARGIGRG IALTLAGAGA NILLADLLDD ALDATAREVR ALGRRAAIAK VDVTQAAQVD AMVAQALADL GGLDILVNCA GVISIHPVAE LTERDWDFVM NVNAKGTFLG CRAALAHLKA QGRGRIINVA SIAGKEGFPN LAHYSASKFA VVGFTNALAK ELARDGVTVN AICPGIVRTY MWDRLSDEWK TDGESVEQSW QRHQLTLIPQ GRAQTPEDMG RLALFFATMD NVTGQAVNVD GGFTFH

Validated NT Sequence

atcttactca cttagtggaa cgtgaagccg ccgtcgacgt tcacggcctg gcccgtcacg ttgtccatcg tcgcgaagaa cagcgcgagc cggcccatgt cctccggcgt ctgcgctcgg ccctgcggaa tcagcgtcaa ctggtgccgc tgccacgact gctcgaccga ttcgccgtcg gtcttccatt cgtcggacag ccggtcccac atgtaggtac ggacgatgcc ggggcagatc gcgttgacgg tcacgccgtc gcgcgcgagt tccttcgcca gcgcgttggt gaagcccacg accgcgaatt tcgatgcgct gtaatgcgcg agattcggaa agccttcctt gcccgcgatc gacgcgacgt tgatgatccg gccgcgcccc tgcgccttca ggtgggcaag cgccgcccgg cagccgagga acgtgccctt cgcgttcacg ttcatcacga aatcccagtc gcgctcggtc agctcggcga ccggatgaat gctgatcacg cccgcgcagt tcacgaggat gtcgagcccg ccgaggtcgg cgagcgcctg cgcgaccatc gcgtcgacct gcgcggcctg cgtgacgtcg accttcgcga tcgccgcgcg gcggccgagc gcacgcacct cgcgcgcggt ggcgtcgagc gcgtcgtcga gcaggtcggc cagcaggatg ttggcgccgg cgccggcgag cgtcagcgcg atgccacggc cgatgccgcg cgcgccgccg gtgacgatgg cgacctgtcc ttcgagaggg gctgtcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacag cccctctcga aggacaggtc gccatcgtca ccggcggcgc gcgcggcatc ggccgtggca tcgcgctgac gctcgccggc gccggcgcca acatcctgct ggccgacctg ctcgacgacg cgctcgacgc caccgcgcgc gaggtgcgtg cgctcggccg ccgcgcggcg atcgcgaagg tcgacgtcac gcaggccgcg caggtcgacg cgatggtcgc gcaggcgctc gccgacctcg gcgggctcga catcctcgtg aactgcgcgg gcgtgatcag cattcatccg gtcgccgagc tgaccgagcg cgactgggat ttcgtgatga acgtgaacgc gaagggcacg ttcctcggct gccgggcggc gcttgcccac ctgaaggcgc aggggcgcgg ccggatcatc aacgtcgcgt cgatcgcggg caaggaaggc tttccgaatc tcgcgcatta cagcgcatcg aaattcgcgg tcgtgggctt caccaacgcg ctggcgaagg aactcgcgcg cgacggcgtg accgtcaacg cgatctgccc cggcatcgtc cgtacctaca tgtgggaccg gctgtccgac gaatggaaga ccgacggcga atcggtcgag cagtcgtggc agcggcacca gttgacgctg attccgcagg gccgagcgca gacgccggag gacatgggcc ggctcgcgct gttcttcgcg acgatggaca acgtgacggg ccaggccgtg aacgtcgacg gcggcttcac gttccactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at