SSGCID: Target Page

BuceA.00010.p

Putative acyl-carrier protein reductase

CENTER ID:	BuceA.00010.p
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00010.p.B2.GE36344	Mature protein( BuceA.00010.p )		21	238

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.888
RefSeq:	YP_002230000.1
UniProt:	B4EAV7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRALVTGGSG ALGQAICTAL AQAGHEVWVH ANRHLAQAEA VAQQIVAAGG AAHAIAFDVT DADATLAALQ PFIDDAPVQI LVNNAGIHDD APMAGMSRRQ WHSVIDVTLN GFFNVTQPLL LPMIRTRRGR IVNIASVAGV TGNRGQANYA AAKAGLIGAT KSLSLELASR GITVNAVAPG IIASPMAEQA FPAERIKQLV PAQRAGRPDE VAAMVAYLVS DAAAYVTGQV LSVNGGLA

NT Sequence

ATGCGGGCGC TCGTGACGGG CGGCAGCGGT GCGCTGGGGC AGGCGATCTG CACGGCGCTC GCGCAGGCCG GCCATGAAGT GTGGGTGCAT GCGAACCGCC ATCTCGCGCA GGCGGAGGCG GTCGCGCAGC AGATCGTCGC GGCCGGCGGC GCCGCGCACG CGATCGCGTT CGACGTGACC GACGCCGACG CGACGCTCGC CGCGCTGCAG CCGTTCATCG ACGATGCGCC GGTGCAGATC CTCGTCAACA ACGCGGGCAT CCACGACGAC GCGCCGATGG CCGGCATGTC GCGCCGGCAA TGGCACAGCG TGATCGACGT GACGCTCAAC GGCTTTTTCA ACGTCACGCA GCCGTTGCTG CTGCCGATGA TCCGCACGCG GCGCGGACGG ATCGTCAACA TCGCATCGGT GGCCGGTGTG ACCGGCAATC GCGGGCAGGC CAACTACGCG GCCGCGAAGG CGGGGCTGAT CGGCGCGACG AAGTCGCTGT CGCTGGAGCT CGCGTCGCGC GGCATCACCG TGAACGCGGT GGCGCCGGGC ATCATCGCGT CGCCGATGGC CGAACAGGCG TTTCCCGCCG AACGCATCAA GCAGCTCGTG CCCGCGCAGC GCGCGGGCCG GCCCGACGAA GTCGCGGCGA TGGTCGCGTA TCTCGTGTCC GACGCCGCGG CCTATGTGAC GGGGCAGGTG TTGTCCGTCA ACGGCGGGCT CGCA

Details for BuceA.00010.p.B2.GE36344

HARVESTED ON:	10/23/2012
SEQUENCED ON:	10/26/2012
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA QAGHEVWVHA NRHLAQAEAV AQQIVAAGGA AHAIAFDVTD ADATLAALQP FIDDAPVQIL VNNAGIHDDA PMAGMSRRQW HSVIDVTLNG FFNVTQPLLL PMIRTRRGRI VNIASVAGVT GNRGQANYAA AKAGLIGATK SLSLELASRG ITVNAVAPGI IASPMAEQAF PAERIKQLVP AQRAGRPDEV AAMVAYLVSX AAAYVTGQVL SVNGGLA

Validated NT Sequence

ttttgtttna ctttaagaag gagatatacc atggctcacc accaccacca ccatatggcg caggccggcc atgaagtgtg ggtgcatgcg aaccgccatc tcgcgcaggc ggaggcggtc gcgcagcaga tcgtcgcggc cggcggcgcc gcgcacgcga tcgcgttcga cgtgaccgac gccgacgcga cgctcgccgc gctgcagccg ttcatcgacg atgcgccggt gcagatcctc gtcaacaacg cgggcatcca cgacgacgcg ccgatggccg gcatgtcgcg ccggcaatgg cacagcgtga tcgacgtgac gctcaacggc tttttcaacg tcacgcagcc gttgctgctg ccgatgatcc gcacgcggcg cggacggatc gtcaacatcg catcggtggc cggtgtgacc ggcaatcgcg ggcaggccaa ctacgcggcc gcgaaggcgg ggctgatcgg cgcgacgaag tcgctgtcgc tggagctcgc gtcgcgcggc atcaccgtga acgcggtggc gccgggcatc atcgcgtcgc cgatggccga acaggcgttt cccgccgaac gcatcaagca gctcgtgccc gcgcagcgcg cgggccggcc cgacgaagtc gcggcgatgg tcgcgtatct cgtgtccnac gccgcggcct atgtgacggg gcaggtgttg tccgtcaacg gcgggctcgc ataagtgagt aagataggat ccggctgcta acnaancccc gaangana

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc aggccggcca tgaagtgtgg gtgcatgcga accgccatct cgcgcaggcg gaggcggtcg cgcagcagat cgtcgcggcc ggcggcgccg cgcacgcgat cgcgttcgac gtgaccgacg ccgacgcgac gctcgccgcg ctgcagccgt tcatcgacga tgcgccggtg cagatcctcg tcaacaacgc gggcatccac gacgacgcgc cgatggccgg catgtcgcgc cggcaatggc acagcgtgat cgacgtgacg ctcaacggct ttttcaacgt cacgcagccg ttgctgctgc cgatgatccg cacgcggcgc ggacggatcg tcaacatcgc atcggtggcc ggtgtgaccg gcaatcgcgg gcaggccaac tacgcggccg cgaaggcggg gctgatcggc gcgacgaagt cgctgtcgct ggagctcgcg tcgcgcggca tcaccgtgaa cgcggtggcg ccgggcatca tcgcgtcgcc gatggccgaa caggcgtttc ccgccgaacg catcaagcag ctcgtgcccg cgcagcgcgc gggccggccc gacgaagtcg cggcgatggt cgcgtatctc gtgtccgacg ccgcggccta tgtgacgggg caggtgttgt ccgtcaacgg cgggctcgca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat