BuceA.00010.o
Putative Acetoacetyl-CoA reductase
| CENTER ID: | BuceA.00010.o |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00010.o.B1.GE36343 | Full length( BuceA.00010.o ) | 1 | 247 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4K6C
DEPOSITED: 4/15/2013
DETERMINATION: XRay
CLONE: BuceA.00010.o.B1.GE36343
PROTEIN: BuceA.00010.o.B1.PS01734
4K6F
DEPOSITED: 4/15/2013
DETERMINATION: XRay
CLONE: BuceA.00010.o.B1.GE36343
PROTEIN: BuceA.00010.o.B1.PS01734
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129142 |
| PATRIC ID: | fig|216591.5.peg.874 |
| RefSeq: | YP_002229987.1 |
| UniProt: | B4EAU4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKRIAVVTG GMGGLGEAVS IRLNDAGHRV VVTYSPNNTG ADRWLTEMHA AGREFHAYPV DVADHDSCQQ CIEKIVRDVG PVDILVNNAG ITRDMTLRKL DKVNWDAVIR TNLDSVFNMT KPVCDGMVER GWGRIVNISS VNGSKGSVGQ TNYAAAKAGM HGFTKSLALE IARKGVTVNT VSPGYLATKM VTAIPQDILD TKILPQIPAG RLGKPEEVAA LVAYLCSEEA GFVTGSNIAI NGGQHMH
NT Sequence
ATGACTAAGC GAATTGCAGT TGTGACGGGT GGGATGGGCG GCCTCGGCGA AGCGGTCAGC ATCAGGTTGA ACGACGCGGG TCATCGGGTA GTCGTCACGT ATTCGCCGAA CAACACCGGC GCGGACCGCT GGCTGACCGA GATGCATGCG GCCGGCCGCG AATTCCATGC GTATCCGGTG GACGTGGCCG ATCACGATTC ATGCCAGCAA TGCATCGAGA AGATCGTGCG GGACGTCGGC CCGGTCGACA TTCTCGTGAA CAATGCGGGC ATCACGCGCG ACATGACGCT GCGCAAGCTC GACAAGGTCA ACTGGGATGC CGTGATCCGC ACCAACCTCG ACTCCGTGTT CAACATGACG AAGCCTGTCT GCGACGGCAT GGTCGAGCGC GGCTGGGGCC GCATCGTCAA CATCTCGTCG GTGAACGGCT CGAAGGGGTC GGTCGGCCAG ACCAACTATG CGGCCGCGAA GGCCGGCATG CACGGCTTCA CGAAATCGCT CGCGCTGGAG ATCGCGCGCA AGGGCGTGAC GGTGAACACG GTGTCGCCCG GCTATCTCGC GACGAAGATG GTCACCGCGA TCCCGCAGGA CATCCTCGAC ACGAAGATCC TCCCGCAGAT TCCGGCGGGC CGGCTCGGCA AGCCCGAGGA AGTCGCGGCG CTGGTGGCCT ATCTGTGCTC GGAGGAGGCC GGCTTCGTGA CGGGCTCCAA CATCGCGATC AACGGCGGTC AGCACATGCA C
Details for BuceA.00010.o.B1.GE36343
| HARVESTED ON: | 10/23/2012 |
| SEQUENCED ON: | 10/26/2012 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT KRIAVVTGGM GGLGEAVSIR LNDAGHRVVV TYSPNNTGAD RWLTEMHAAG REFHAYPVDV ADHDSCQQCI EKIVRDVGPV DILVNNAGIT RDMTLRKLDK VNWDAVIRTN LDSVFNMTKP VCDGMVERGW GRIVNISSVN GSKGSVGQTN YAAAKAGMHG FTKSLALEIA RKGVTVNTVS PGYLATKMVT AIPQDILDTK ILPQIPAGRL GKPEEVAALV AYLCSEEAGF VTGSNIAING GQHMH
Validated NT Sequence
atcttactca cttagtgcat gtgctgaccg ccgttgatcg cgatgttgga gcccgtcacg aagccggcct cctccgagca cagataggcc accagcgccg cgacttcctc gggcttgccg agccggcccg ccggaatctg cgggaggatc ttcgtgtcga ggatgtcctg cgggatcgcg gtgaccatct tcgtcgcgag atagccgggc gacaccgtgt tcaccgtcac gcccttgcgc gcgatctcca gcgcgagcga tttcgtgaag ccgtgcatgc cggccttcgc ggccgcatag ttggtctggc cgaccgaccc cttcgagccg ttcaccgacg agatgttgac gatgcggccc cagccgcgct cgaccatgcc gtcgcagaca ggcttcgtca tgttgaacac ggagtcgagg ttggtgcgga tcacggcatc ccagttgacc ttgtcgagct tgcgcagcgt catgtcgcgc gtgatgcccg cattgttcac gagaatgtcg accgggccga cgtcccgcac gatcttctcg atgcattgct ggcatgaatc gtgatcggcc acgtccaccg gatacgcatg gaattcgcgg ccggccgcat gcatctcggt cagccagcgg tccgcgccgg tgttgttcgg cgaatacgtg acgactaccc gatgacccgc gtcgttcaac ctgatgctga ccgcttcgcc gaggccgccc atcccacccg tcacaactgc aattcgctta gtcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMT KRIAVVTGGM GGLGEAVSIR LNDAGHRVVV TYSPNNTGAD RWLTEMHAAG REFHAYPVDV ADHDSCQQCI EKIVRDVGPV DILVNNAGIT RDMTLRKLDK VNWDAVIRTN LDSVFNMTKP VCDGMVERGW GRIVNISSVN GSKGSVGQTN YAAAKAGMHG FTKSLALEIA RKGVTVNTVS PGYLATKMVT AIPQDILDTK ILPQIPAGRL GKPEEVAALV AYLCSEEAGF VTGSNIAING GQHMH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta agcgaattgc agttgtgacg ggtgggatgg gcggcctcgg cgaagcggtc agcatcaggt tgaacgacgc gggtcatcgg gtagtcgtca cgtattcgcc gaacaacacc ggcgcggacc gctggctgac cgagatgcat gcggccggcc gcgaattcca tgcgtatccg gtggacgtgg ccgatcacga ttcatgccag caatgcatcg agaagatcgt gcgggacgtc ggcccggtcg acattctcgt gaacaatgcg ggcatcacgc gcgacatgac gctgcgcaag ctcgacaagg tcaactggga tgccgtgatc cgcaccaacc tcgactccgt gttcaacatg acgaagcctg tctgcgacgg catggtcgag cgcggctggg gccgcatcgt caacatctcg tcggtgaacg gctcgaaggg gtcggtcggc cagaccaact atgcggccgc gaaggccggc atgcacggct tcacgaaatc gctcgcgctg gagatcgcgc gcaagggcgt gacggtgaac acggtgtcgc ccggctatct cgcgacgaag atggtcaccg cgatcccgca ggacatcctc gacacgaaga tcctcccgca gattccggcg ggccggctcg gcaagcccga ggaagtcgcg gcgctggtgg cctatctgtg ctcggaggag gccggcttcg tgacgggctc caacatcgcg atcaacggcg gtcagcacat gcactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat