BuceA.00010.k
Putative dehydrogenase
| CENTER ID: | BuceA.00010.k |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00010.k.B1.GE36339 | Full length( BuceA.00010.k ) | 1 | 257 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5THK
DEPOSITED: 9/29/2016
DETERMINATION: XRay
CLONE: BuceA.00010.k.B1.GE36339
PROTEIN: BuceA.00010.k.B1.PW37237
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|216591.5.peg.2788 |
| RefSeq: | YP_002231797.1 |
| UniProt: | B4E8W7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATHTLADKV VLIAGGAKNL GGLIARDLAG HGAKAVAIHY NSAASQAQAE ETAAAVRAAG AEAATFQADL TTAAAVEKLF DDAKQRFGKI DIAINTVGKV LKKPFTEISE AEYDEMFAVN SKSAFFFIKE AGRHLEDHGK LVTLVTSLLG AFTPFYAAYE GSKAPVEHFT RAASKEYGAR GISVTAVGPG PMDTPFFYPA EGADAVAYHK TAAALSPFSK TGLTDIEDVV PFIRHLVTDG WWITGQTILI NGGYTTK
NT Sequence
GTGGCAACTC ATACGCTCGC AGACAAGGTC GTCCTGATCG CAGGCGGGGC AAAGAATCTC GGTGGCCTGA TCGCCCGGGA CCTGGCGGGC CACGGCGCGA AGGCGGTGGC GATTCACTAC AACAGCGCGG CATCGCAGGC GCAGGCCGAG GAGACCGCCG CGGCGGTCCG TGCGGCTGGC GCGGAAGCCG CGACGTTCCA GGCGGACCTG ACGACGGCCG CCGCGGTCGA GAAGCTGTTC GACGACGCGA AGCAGCGCTT CGGCAAGATC GACATCGCGA TCAACACGGT CGGCAAGGTG CTGAAGAAGC CGTTCACCGA GATCAGCGAG GCCGAGTACG ACGAGATGTT CGCGGTCAAC AGCAAGTCGG CGTTCTTCTT CATCAAGGAA GCAGGCCGGC ATCTCGAGGA TCACGGCAAG CTCGTCACGC TCGTGACGTC GCTGCTCGGC GCGTTCACGC CGTTCTACGC GGCGTACGAA GGGTCGAAGG CGCCGGTCGA GCACTTCACG CGCGCGGCGT CGAAGGAGTA CGGCGCGCGC GGGATCTCGG TGACGGCCGT CGGGCCGGGC CCGATGGACA CGCCGTTCTT CTATCCGGCC GAAGGCGCCG ACGCGGTCGC GTATCACAAG ACGGCCGCCG CGCTGTCGCC GTTCAGCAAG ACGGGGCTGA CCGACATCGA GGACGTCGTC CCGTTCATCC GCCATCTGGT GACCGACGGC TGGTGGATCA CCGGCCAGAC GATCCTGATC AACGGCGGCT ACACGACCAA G
Details for BuceA.00010.k.B1.GE36339
| HARVESTED ON: | 10/23/2012 |
| SEQUENCED ON: | 10/26/2012 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV ATHTLADKVV LIAGGAKNLG GLIARDLAGH GAKAVAIHYN SAASQAQAEE TAAAVRAAGA EAATFQADLT TAAAVEKLFD DAKQRFGKID IAINTVGKVL KKPFTEISEA EYDEMFAVNS KSAFFFIKEA GRHLEDHGKL VTLVTSLLGA FTPFYAAYEG SKAPVEHFTR AASKEYGARG ISVTAVGPGP MDTPFFYPAE GADAVAYHKT AAALSPFSKT GLTDIEDVVP FIRHLVTDGW WITGQTILIN GGYTTK
Validated NT Sequence
atcttactca cttacttggt cgtgtagccg ccgttgatca ggatcgtctg gccggtgatc caccagccgt cggtcaccag atggcggatg aacgggacga cgtcctcgat gtcggtcagc cccgtcttgc tgaacggcga cagcgcggcg gccgtcttgt gatacgcgac cgcgtcggcg ccttcggccg gatagaagaa cggcgtgtcc atcgggcccg gcccgacggc cgtcaccgag atcccgcgcg cgccgtactc cttcgacgcc gcgcgcgtga agtgctcgac cggcgccttc gacccttcgt acgccgcgta gaacggcgtg aacgcgccga gcagcgacgt cacgagcgtg acgagcttgc cgtgatcctc gagatgccgg cctgcttcct tgatgaagaa gaacgccgac ttgctgttga ccgcgaacat ctcgtcgtac tcggcctcgc tgatctcggt gaacggcttc ttcagcacct tgccgaccgt gttgatcgcg atgtcgatct tgccgaagcg ctgcttcgcg tcgtcgaaca gcttctcgac cgcggcggcc gtcgtcaggt ccgcctggaa cgtcgcggct tccgcgccag ccgcacggac cgccgcggcg gtctcctcgg cctgcgcctg cgatgccgcg ctgttgtagt gaatcgccac cgccttcgcg ccgtggcccg ccaggtcccg ggcgatcagg ccaccgagat tctttgcccc gcctgcgatc aggacgacct tgtctgcgag cgtatgagtt gccaccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMV ATHTLADKVV LIAGGAKNLG GLIARDLAGH GAKAVAIHYN SAASQAQAEE TAAAVRAAGA EAATFQADLT TAAAVEKLFD DAKQRFGKID IAINTVGKVL KKPFTEISEA EYDEMFAVNS KSAFFFIKEA GRHLEDHGKL VTLVTSLLGA FTPFYAAYEG SKAPVEHFTR AASKEYGARG ISVTAVGPGP MDTPFFYPAE GADAVAYHKT AAALSPFSKT GLTDIEDVVP FIRHLVTDGW WITGQTILIN GGYTTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgg caactcatac gctcgcagac aaggtcgtcc tgatcgcagg cggggcaaag aatctcggtg gcctgatcgc ccgggacctg gcgggccacg gcgcgaaggc ggtggcgatt cactacaaca gcgcggcatc gcaggcgcag gccgaggaga ccgccgcggc ggtccgtgcg gctggcgcgg aagccgcgac gttccaggcg gacctgacga cggccgccgc ggtcgagaag ctgttcgacg acgcgaagca gcgcttcggc aagatcgaca tcgcgatcaa cacggtcggc aaggtgctga agaagccgtt caccgagatc agcgaggccg agtacgacga gatgttcgcg gtcaacagca agtcggcgtt cttcttcatc aaggaagcag gccggcatct cgaggatcac ggcaagctcg tcacgctcgt gacgtcgctg ctcggcgcgt tcacgccgtt ctacgcggcg tacgaagggt cgaaggcgcc ggtcgagcac ttcacgcgcg cggcgtcgaa ggagtacggc gcgcgcggga tctcggtgac ggccgtcggg ccgggcccga tggacacgcc gttcttctat ccggccgaag gcgccgacgc ggtcgcgtat cacaagacgg ccgccgcgct gtcgccgttc agcaagacgg ggctgaccga catcgaggac gtcgtcccgt tcatccgcca tctggtgacc gacggctggt ggatcaccgg ccagacgatc ctgatcaacg gcggctacac gaccaagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at